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        검색결과 242

        62.
        2019.04 구독 인증기관·개인회원 무료
        Melon thrips, Thrips palmi Karny (Thysanoptera: Thripidae) is a worldwide polyphagous pest. The management of this pest have mainly relied on chemical agents. However, the overuse is harmful to the environment and results in insects resistance. Therefore, alternative eco-friendly control methods that have different mode of action, such as biological control, are necessary to overcome the current issue. In this study, we isolated entomopathogenic fungi from Korean soil, and characterized them via morphological and molecular techniques and pathogenicity assay against Tenebrio molitor larvae. The isolated fungi were screened for virulence against T. palmi under laboratory conditions and the results were used to establish a thrips-pathogenic fungal library. The highly virulent isolates were selected and further characterized for optimum culture conditions and application as biopesticide in the field.
        63.
        2019.04 구독 인증기관·개인회원 무료
        Developmental characteristics of immature stages of Ostrinia scapulalis (Lepidoptera: Crambidae), which is a main insect pest against legume crops, were investigated at constant temperatures to prepare fundamental data for integrated pest management. Egg, larva and pupa could survive at temperatures from 16oC to 36oC. Developmental periods of those stages became shorter with increasing temperature between 16 and 31oC, 16 and 36oC, and 16 and 34oC, respectively. The lower developmental thresholds and degree-days(DD) were determined as 13.0oC and 56.9DD, 13.8oC and 277.5DD, and 14.2oC and 90.8DD using a linear regression model between temperature and development rate. Other parameters for explaining development of this species were estimated using a few non-linear models for developmental rate, distribution, and survivorship.
        64.
        2019.04 구독 인증기관·개인회원 무료
        A species of Beauveria bassiana is widely used for biological pest management in many countries. Many efforts have been given to figure out the clear fungal mode of action to enhance the insecticidal activity. Homologous recombination (knock-out) or hairpin RNA (knock-down) is popularly used in fungal gene function study, but gene cloning and generation of knock-out or -down mutants takes long time or temporarily knock-downed. Here in this work, we used previously generated egfp-expressing B. bassiana strain (Bb-egfp #3) and integrated dsegfp to the Bb-egfp #3 using a protoplast integration method. This work suggests that protoplast integration with dsRNA possibly generate significantly reduced gene expression in B. bassiana and the reduction is quite stable over generations which provide easy of functional study for fungal mode of action.
        65.
        2019.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        고온기 화색발현이 우수하고 연중생산이 가능한 수출용 스프레이국화 신품종을 육성하기 위하여 충남농업기술원 화훼 연구소에서 2010년 분홍색의 모본 ‘Borami’를 방임수분하였다. 2011년에 종자를 파종하였고, 이중 화형과 화색이 우수한 개체를 선발하여 ‘SP11-148-01’로 계통명을 부여하였다. 2011년부터 2013년까지 주년 생산성을 위해 전조, 자연, 차광재배로 특성을 각각 검정하였고, 생육 및 개화특성은 화형과 화색이 비슷한 자주색 스프레이국화인 ‘Kingfisher’를 대조품종으로 조사하였으며, 2013년 ‘Yes Ruby’로 품종등록 출원하였다. ‘Yes Ruby’는 자주색의 설상화와 연녹색의 통상화로 가을 작형 개화기는 10월 24일로, ‘Kingfisher’의 10월 29일에 비해 빨랐다. ‘Yes Ruby’의 초장과 줄기굵기는 각각 94.9cm와 7.7mm로 ‘Kingfisher’의 89.2cm와 6.4mm보다 컸다. ‘Yes Ruby’의 꽃 직경은 6.2cm로 ‘Kingfisher’의 5.0cm보다 컸으며, 꽃잎수도 ‘Yes Ruby’가 25.7개로 ‘Kingfisher’의 23.3개보다 많았다. 착화수는 두 품종 모두 비슷하였으며, 흰녹병 저항성은 ‘Yes Ruby’가 2단계, ‘Kingfisher’는 3단계의 감염 정도를 나타내어 흰녹병에 대한 저항성이 높은 것으로 나타났다. 재배상 유의사항은 ‘Yes Ruby’는 겨울철 균일한 개화가 이뤄지지 않아 겨울철 야간온도를 18℃ 이상으로 관리해줌으로써 균일개화를 유도할 수 있다. 또한 생장억제제인 Daminozide을 처리함으로써 설상화수를 늘려 볼륨감 높은 꽃봉오리를 형성할 수 있어 고온기에도 화색발현이 우수하고 연중생산이 가능하여 안정적 수출을 통한 농가소득 증대에 기여할 수 있을 것으로 기대된다.
        4,000원
        67.
        2018.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 국내 플라워샵에서의 절화보존제 사용현황 및 인식을 알아보고자 국내 성인남녀 120명의 플로리스트들을 상대로 설문조사를 진행하였다. 국내 플라워샵에서 일하는 플로리스트들 중 19.8%만이 절화보존제를 사용하는 것으로 나타난 반면, 전혀 사용하지 않은 플라워샵은 45.5%로, 국내 플라워샵에서의 절화보존제 이용이 매우 낮은 것으로 나타났다. 국내 플로리스트들의 절화보존제 주 사용 시기와 목적은 ‘화훼 상품 보관 시(39.6%)’에 ‘균 번식 억제를 위해(36.7%)’ 가장 많이 사용하는 것으로 나타났으며 절화보존제를 사용하지 않는 이유에 대해서는 필요성을 느끼지 못한다는 응답(51.4%)이 가장 높게 나타나, 절화보존제에 대한 인식이 부족한 것으로 나타났다. 그러나, 절화보존제 사용 경험이 있는 플로리스트들에게서는 절화보존제의 이용 만족도가 높게 나타났으며, 50.5%의 절화보존제 이용 경험자는 재구매 의사가 매우 높은 것으로 나타났다. 일반적으로 국내 플로리스트들의 경우 절화보존제에 대한 관심이 적지는 않으나(Mean=3.22), 절화보존제의 적은 이용 빈도와 낮은 인지도는 국내 절화보존제 시장이 작고 홍보가 부족하여 나타난 것으로 판단된다. 절화보존제 사용 후 플로리스트들의 높은 만족도와 재구매의사를 보았을 때, 절화보존제에 대한 홍보와 교육을 통하여 국내 플라워샵에서의 절화수명제의 접근성을 높이고 절화 품질 관리에 대한 인식을 고양시킬 수 있을 것이며, 이를 통해 소비자들에게도 고품질의 관상기간이 긴 절화 상품 제공을 통하여 국내 절화산업의 발전을 가져올 수 있을 것이다.
        4,000원
        70.
        2018.11 구독 인증기관·개인회원 무료
        Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Recently, polymorphisms reported to be significant association with sperm MOT. This study was conducted to evaluate the SNP in the coding region of ESR1 (g.672C>T inexon 1) as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results,The g.672C>T was significantly associated with frozen semen motility and kinematic characteristics. g.158 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP Also, the SNP was low significantly associated with ALH.Therefore, we suggest that theSNP in the coding region of ESR1 (g.672C>T in exon 1) may be used as a molecular marker for Duroc boar Post-thawed semen quality.
        71.
        2018.11 구독 인증기관·개인회원 무료
        Estrogen receptor 2 (ESR2) is involved in oestrogen-related apoptosis in cell cycle spermatogenesis but their effects have not yet confirmed in pig. Therefore, this study was performed to investigatetheir association with semen motility and kinematic characteristics. DNA samples from 126 Duroc pigs with records of sperm motility and kinematic characteristics [Total motile spermatozoa (MOT), Curvilinear velocity(VCL), Straight-line velocity(VSL), the ratio between VSL and VCL(LIN), Amplitude of Lateral Head displacement(ALH)] were used in present study. A single nucleotide polymorphism (g.35547A>G) was associated with MOT, VCL, VAP and ALH in Duroc population (p < 0.05). Therefore, we suggest that the porcine ESR2 may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not clear yet. These results will improve the understanding of the functions of the ESR2 in spermatogenesis within the reproductive tracts, and will shed light on ESR2 as a candidate gene in the selection of good sperm quality boars.
        72.
        2018.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The purpose of this study is to promote the elderly apparel industry for the increasing numbers of elderly obese male population. In the study, a total number of 249 males between the ages of 60 to 85 were studied to analyze their body types and differences. The group had a Rohrer Index of 1.6 or higher and BMI of 25 or higher. The noticeable physical differences in the group were shorter waist front length, bigger waist and hip circumferences with increasing age and slimmer limbs that are associated with the natural aging process with or without obesity. The obese body types have been classified in the following 3 different categories. Type 1 is the group that has lower body obesity with broad shoulders and relatively slimmer abdomen than a heavy bottom. A total number of 84 people belonged to the type 1 obesity category which makes up 33.8% of the total. Type 2 is the group that has upper body obesity with especially large abdominal obesity. A total number of 76 people, 30.5% of the total, were classified as type 2. Type 3 is the group that has whole body obesity with balanced obesity in the whole body. A total number of 89 people, 35.7% of the total, made up type 3.
        4,600원
        73.
        2018.10 구독 인증기관·개인회원 무료
        Insect-killing fungi have high potential in pest management. A deeper insight into the fungal genes at the whole genome level is necessary to understand the inter-species or intra-species genetic diversity of fungal genes, and to select excellent isolates. In this work, we conducted a whole genome sequencing of Beauveria bassiana (Bb) JEF-007 and characterized pathogenesis-related features and compared with other isolates including Bb ARSEF2860. A large number of Bb JEF-007 genes showed high identity with Bb ARSEF2860, but some genes showed moderate or low identity. The two Bb isolates showed a significant difference in vegetative growth, antibiotic-susceptibility, and virulence against Tenebrio molitor larvae. When highly identical genes between the two Bb isolates were subjected to real-time PCR, their transcription levels were different, particularly in heat shock protein 30 (hsp30) gene which is related to conidial thermotolerance. In several B. bassiana isolates, chitinases and trypsin-like protease genes involved in pathogenesis were highly conserved, but other genes showed noticeable sequence variation within the same species. Given the transcriptional and genetic diversity in B. bassiana, a selection of virulent isolates with industrial advantages is a pre-requisite, and this genetic approach could support the development of excellent biopesticides with intellectual property protection.
        74.
        2018.10 구독 인증기관·개인회원 무료
        소나무재선충병의 나무주사는 살선충제를 중심으로 실시되어 왔으며, 매개충인 솔수염하늘소와 북방수염하늘소의 방제는 주로 항공방제나 지상방제를 통하여 이루어졌다. 본 연구에서는 나무주사를 통하여 매개충 (솔수염하늘소)을 방제하기 위한 연구로 Abamectin+Acetamiprid ME, Thiamethoxam DC 약제의 나무주사시 솔수염하늘소의 약효발현농도, 처리 후 기간별 효과(2017년~2018년)를 검토하였다. 실내 발현농도 시험에서는 Acetamiprid, Thiamethoxam LC50 value는 각각 0.102ppm, 0.083ppm 으로 나타 났으며, 야산(포장)에서 Abamectin+Acetamiprid ME, Thiamethoxam DC 처리구의 100.0% 치사 소요일 수는 처리 90일 후 11.0일, 9.4일, 처리 360일 후 11.6일, 10.0일로 나타나서 두 약제 모두 3월 처리시 다음해에 발생하는 매개충 (솔수염하늘소) 방제까지 가능함을 확인 할 수 있었다.
        75.
        2018.10 구독 인증기관·개인회원 무료
        The Asian corn borer, O. furnacalis (Crambidae) and the old world bollworm, H. armigera (Noctuidae) simultaneously cause ear damage in corn in Suwon area. It has been assumed that the second generation of larvae of both insects is responsible for the damage. We, therefore, studied proper spray timing of insecticide in order to reduce the damage. A waxy corn hybrid cultivar, Ilmichal, was sown directly on four plots of a field at April 10, April 20, May 4, and May 18, 2018, respectively. A sodium channel blocker, indoxacarb, was sprayed eight times at 3- or 4-day intervals around the silking stage of corn in each sowing plot. At ear harvest time of each plot, we investigated damaged ratio and length of ear, and the numbers of both insect species. The significantly effective spray timings on reduction of ear damage were the sprays at June 29 and July 2 in the plot sown at April 10, the sprays from July 2 to 13 in the April 20-sown plot, the sprays from July 2 to 19 in the May 4-sown plot, and the sprays at July 19 in the May 18-sown plot, respectively. The damage level was positively related to the insect number. The result indicated that the spray at the silking stage of corn was effective on reduction of ear damage. It was assumed that occurrence time of neonate larvae of the insects at silking stage cause the damage, based on the experimental results using screened pots inoculated with the two species.
        76.
        2018.10 구독 인증기관·개인회원 무료
        The genus Nipponopsyche Yazaki is reported from Korea with an unrecorded species, N. fuscescens Yazaki for the first time. Adult, larva, pupa and genitalia of the species are redescribed with SEM illustrations, and DNA barcode for precise identification of the species is also provided.
        77.
        2018.10 구독 인증기관·개인회원 무료
        흑백알락나비 (Hestina persimilis (Westwood, 1850))는 한반도의 평안남도 이남의 내륙지역에 넓게 분포하고, 국외의 경우 일본, 중국, 히말라야 일대에 서식하는 것으로 알려져 있다. 본 종에 대한 생활사 연구는 김과 서(2011)에 의해 월동유충, 종령, 번데기 등에 대한 일부 보고가 있을 뿐 자세한 생활사에 대한 기록은 전무하다. 본 연구를 통해 한국산 흑백알락나비의 생태적 습성 및 미성숙단계 (알, 유충, 번데기)의 형태적 특징 등 자세한 생활사 정보를 보고하고자 한다.
        78.
        2018.10 구독 인증기관·개인회원 무료
        The continuous emergence of chemical-mediated residual toxicity and insect resistance have enforced the regulation of synthetic pesticides. Synthetic pesticides with novel mode of actions could be developed to overcome these issues, but as an alternative biopesticides with more efficacious control activity could be developed by the advanced technology. In pest management, entomopathogenic fungi have high potential in reducing pest population to an economic threshold, and some of isolates have been commercialized. However still a novel application strategy needs to be considered for successful industrialization. An insight on fungal genes in whole genome and transcriptome levels is necessary to understand the role of genes in pathogenesis and genetic diversity of fungal genes. Herein, we have identified the genetic differences of entomopathogenic fungi using whole genome sequencing of Beauveria bassiana (Bb) and tried to understand the interaction between fungus and insect using RNA-seq. We have obtained the whole genome sequence of Bb JEF-007 using PacBio sequencing technology and compared the transcriptomes of Bb JEF-007 and bean bug, Riptortus pedestris before and after the fungal infection using Hiseq 2000 system.
        79.
        2018.10 구독 인증기관·개인회원 무료
        Entomopathogenic fungi have been known as promising candidates for biological control of insect pests. Recently, researchers consider the fungal thermotolerance in formulations and field applications. In this study, we investigated the production of thermotolerant Isaria javanica and I.fumosorosea conidia through grain-based solid cultures and exposure to light stress. As results, of the ten grain substrates, Italian millet, rice, perilla seed and sesame, rice, sorghum produced highly thermotolerant conidia in the strains. The two strains were exposed to a light stress and showed enhanced thermal stability compared to control, when exposed to 45°C for 2 hours. This work suggests that heatresistant entomopathogenic fungal conidia can be produced by grainbased solid cultures and exposure to light stress.
        80.
        2018.10 구독 인증기관·개인회원 무료
        과일이나 농작물의 부패 및 발효 환경에서는 Methanol, Ethanol, Acetic acid을 비롯한 다양한 화학물질들이 생산된다. Drosophila melanogaster는 이러한 발효·부패 환경에 서식하면서 일정 농도 이상의 다양한 화학물질에 지속적으로 노출되어 생존하도록 적응되어온 것으로 생각된다. 다양한 화학물질이 포함한 환경에 안정적으로 서식하기 위해서는 D. melanogaster는 화학물질에 능동적으로 반응하여 해독 유전자나 대사 관련 유전자의 발현량을 변화 시킴으로써 발효·부패 환경에서 생성되는 화학물질에 대한 높은 내성을 가지고 있을 것으로 판단된다. 현재까지 유전자의 발현량 측정을 위해 real-time PCR를 이용하여 reference gene의 발현량을 기준으로 정량화하는 방법이 가장 널리 사용되고 있다. 그러나 조직별, 환경별, 발달단계를 비롯한 다양한 조건에서 안정적으로 발현되는 reference 유전자 선정이 필수적으로 선행되어야 하므로 본 연구에서는 발효·부패 환경에서 생산되는 두 화학물질인 Methanol과 Ethyl Acetate에 노출된 D. melanogaster에서 안정적으로 발현되는 reference gene을 찾는 연구를 실시하였다. 본 연구에서는 다양한 농도의 Methanol과 Ethyl Acetate을 D. melanogaster에 노출시킨 후 RNA 추출과 cDNA 합성을 실시였고, 5가지 후보 reference gene (hsp22, nd, rpL18, tbp and ef-1b)의 안정적 발현 여부를 qRT-PCR을 통해 조사하였으며, 유전자 발현의 안정성을 측정하는 3가지 프로그램(geNorm, NormFinder, BestKeeper)을 이용해 비교·분석하였다. 본 학회에서는 연구의 과정과 그 결과를 발표하고자 한다.
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