A long term monitoring was carried out to compare altitudinal effects on insect community structures on high mountains and evaluate responses the monitoring and changed of insect communities induced by the climate change. These mountains were choose for Mt. Jeombong for northern part, Mt. Ilwol for middle and Mt. Beakun for southern. Each mountain was divided into three altitudinal gradients. They were collected three times a season from spring to autumn, using pitfall traps for ground beetles and UV light trap for moths. The present study presents preliminary results of analysis for the first year monitoring. In total 41beetle species and 326 moths were collected from the monitoring sites in 2012. abundance of ground beetles and moths were the highest in Mt. Baekun followed by Mt. Ilwol and lowest in Mt. Jeombong. Analysis of variance (ANOVA) results showed statistically significant differences among sampling area, species evenness and Shannon’s diversity index with altitude in species abundance as a response variable. Also we found statistically significant differences to three species of ground beetle and six species of moths with altitude. Although we expected a distinct cluster with the difference of altitude at each study site. one of ordination analysis, nonmetric multidimensional scaling (NMS), showed distinct clusters with the ground beetles assemblage at some altitude and moths assemblage at sampling date.

The objective of this study was to examine the effects of auditory biofeedback training and kicking training on walking times in patients with hemiplegia to determine if the effects of such training would be maintained even after stopping the intervention. Thirty hemiplegia patients were selected and randomly assigned to a control group of 10 patients to receive general exercise treatment; an auditory biofeedback training group of 10 patients to receive auditory biofeedback training, along with general exercise; and a kicking training group to receive kicking training, along with general exercise. All the patients received their respective training 12 times in total, three times per week for four weeks. In addition, all those in the control and experimental groups received the same general exercise treatment 12 times in total, three times per week for four weeks, and underwent follow-up tests thereafter. The patients’10m walking times were measured using a stopwatch. The significance was analyzed using repeated-ANOVAs. In cases where there were interactions between measuring times and groups, in each group were examined using repeated-ANOVAs. In cases where there were differences, post-hoc tests were conducted using repeated of contrast test. The 10m walking times of the control and experimental groups were significant differences in 10m walking times were shown between measurement times(p<.05), and significant differences in the interactions between measuring times and groups were shown between the groups(p <.05). However, no significant differences in 10m walking times were shown between the groups(p>.05). The auditory biofeedback training group showed significant decreases in walking times four weeks after the beginning of the intervention(p<.05) and significant increases eight weeks after the beginning of the intervention(p<.05). The kicking training group showed significant decreases in walking time four weeks after the beginning of the intervention(p<.05) and maintained the walking times without showing any significant differences eight weeks after the beginning of the intervention(p>.05). The walking speeds of only the kicking training group were maintained until eight weeks after the beginning of the intervention.

The objective of this study was to examine the effects of auditory biofeedback training and kicking training on walking times in patients with hemiplegia to determine if the effects of such training would be maintained even after stopping the intervention. Thirty hemiplegia patients were selected and randomly assigned to a control group of 10 patients to receive general exercise treatment; an auditory biofeedback training group of 10 patients to receive auditory biofeedback training, along with general exercise; and a kicking training group to receive kicking training, along with general exercise. All the patients received their respective training 12 times in total, three times per week for four weeks. In addition, all those in the control and experimental groups received the same general exercise treatment 12 times in total, three times per week for four weeks, and underwent follow-up tests thereafter. The patients’10m walking times were measured using a stopwatch. The significance was analyzed using repeated-ANOVAs. In cases where there were interactions between measuring times and groups, in each group were examined using repeated-ANOVAs. In cases where there were differences, post-hoc tests were conducted using repeated of contrast test. The 10m walking times of the control and experimental groups were significant differences in 10m walking times were shown between measurement times(p<.05), and significant differences in the interactions between measuring times and groups were shown between the groups(p <.05). However, no significant differences in 10m walking times were shown between the groups(p>.05). The auditory biofeedback training group showed significant decreases in walking times four weeks after the beginning of the intervention(p<.05) and significant increases eight weeks after the beginning of the intervention(p<.05). The kicking training group showed significant decreases in walking time four weeks after the beginning of the intervention(p<.05) and maintained the walking times without showing any significant differences eight weeks after the beginning of the intervention(p>.05). The walking speeds of only the kicking training group were maintained until eight weeks after the beginning of the intervention.

The purpose of this study was to implement a program of combined muscle stregth and proprioceptive exercises and to examine the impacts of these exercises on functional ankle instability(FAI). Experiments were conducted with 30 adult males and females in their 20s, and the exercise programs were implemented three days per week for four weeks. FAI was defined as a feeling of giving way after an ankle sprain and having a Cumberland ankle instability tool score of 24 points or less. The study subjects were randomly assigned to either a control group, a muscle-strengthening exercise group, or a combined muscle-strengthening and proprioceptive exercise group consisting of 10 subjects each. A Biodex isokinetic dynamometer was used to assess the subjects’ankle strength at selected speeds of 60°/sec and 120°/sec. The peak torque % body weight showed significant differences in plantar flexion, dorsiflexion, inversion, and eversion. There were also significant differences in proprioception. The results suggest that applying combined muscle-strength and proprioceptive exercises to subjects with FAI is a more effective intervention than applying only muscle-strengthening exercises.

Mealworms, Tenebrio molitor (L.) is used as an important animal feed additive for growth promotion and health management, but potentially exposes to fungal infection. In this work, virulence of two species of entomopathogenic fungi against the insect, and the relationship between abiotic features and virulence were investigated. Secondly our consideration was also given to the effect of chemical fungicides on conidial germination for risk control. Between Beauveria bassiana (Bb) and Metarhizium roberstii (Mr) (previously M. anisopliae), Bb isolates had much higher virulence (~100% mortality in 3~4 days after the treatment), rather than Mr isolates in laboratory assays. Next, fungus-treated mealworms were kept at wheat bran at 20, 25, 30 and 35℃ with 3, 6, 9 times of water spray to the feeds for set-up of different humidity conditions. Inoculation of fungi to mealworms was conducted by fungal spray and feeding methods, which resulted in higher virulence in feeding method. In the feeding method, all temperature treatments except 35℃ showed high virulence against mealworms, but any significant relationship between virulence and humidity was not observed. In the chemical fungicide screening, fluazinam (CAS No. 79622-59-6) and mancozeb (8018-01-7) significantly inhibited the germination of Bb and Mr conidia. This work suggest that contamination of wheat bran with fungal pathogens, particularly B. bassiana may induce mycosis of mealworms, but introduction of effective fungicides possibly reduce fungal infection.

Methanol as a carbon source in chemical vapor deposition (CVD) graphene has an advantage over methane and hydrogen in that we can avoid optimizing an etching reagent condition. Since methanol itself can easily decompose into hydrocarbon and water (an etching reagent) at high temperatures [1], the pressure and the temperature of methanol are the only parameters we have to handle. In this study, synthetic conditions for highly crystalline and large area graphene have been optimized by adjusting pressure and temperature; the effect of each parameter was analyzed systematically by Raman, scanning electron microscope, transmission electron microscope, atomic force microscope, four-point-probe measurement, and UV-Vis. Defect density of graphene, represented by D/G ratio in Raman, decreased with increasing temperature and decreasing pressure; it negatively affected electrical conductivity. From our process and various analyses, methanol CVD growth for graphene has been found to be a safe, cheap, easy, and simple method to produce high quality, large area, and continuous graphene films.

Acteoside acts as an anti-oxidative activity and anti-apoptosis in the cells. But, it has been not studied on maturation and development of porcine oocytes. The aims of the present study were to examine the effects of acteoside on the morphological progress of meiosis, developmental competence, and ROS in porcine oocytes. Oocytes were matured in tissue culture medium-199, supplemented with acteoside at various concentrations: 0 (control), 10, 30 and 50 μM. The oocytes maturation rates of groups supplemented with acteoside were no significantly different (81.13, 85.96, 82.95 and 83.68%, respectively). Level of ROS was significantly decreased in acteoside treated group. Furthermore, the parthenogenetic blastocyst rate was significantly improved in 10 μM acteoside treated group compared with control group (44.83 vs. 27.75%). And we investigated effect of acteoside on the oocytes condition represented by cytoplasmic maturation by homogeneous distribution and formation of cytoplasmic organelles and regulation of apoptosis-related genes. In the results. during IVM, 10 μM acteoside treated oocytes showed that the mitochondria and lipid droplet were smaller and homogeneous distribution in cytoplasm compare with control oocytes. And reverse transcription polymerase chain reaction (RTPCR) of parthenogenetic blstocysts revealed that acteoside increased the anti-apoptotic genes (Mcl-1, Bcl-2 and Bcl-xL), whereas reduced the expression of pro-apoptotic genes (Bax and Bak). In conclusion, based on the results, the effect of acteoside on IVM was not attractive. However, in acteoside treated group, cytoplasmic maturation seemed to be improved with morphologically uniform distribution of cytoplasmic organelles. Furthermore, embryonic development in acteoside treated group was significantly highly increased than that of non-treated group. Our results represents that addition of acteoside to the IVM medium has a beneficial effect in physiology of porcine oocytes, providing a improved method for porcine oocytes in vitro. * This work was supported by a grant (Code# PJ008148) from BioGreen21 Program, Rural Development Administration, Republic of Korea.

The Samia cynthia ricini (Lepidoptera: Saturniidae) is a commercial silk-producing insect belonging to an insect family Saturniidae in Bombycoidea. The species that has presumably been originated in India, is distributed in India, China, and Japan. Unlikely domestic silkworm the prime host plant for the species is a castor-oil plant (Ricinus communis in Euphorbiaceae). Recently, the eri-silkworm also is reared in Korea and is expected to be utilized for a diverse purpose. In this report, we present the complete mitochondrial genome of the species with the emphasis of a few major characteristics. The 15,384-bp long S. cynthia ricini (Lepidoptera: Saturniidae) mitochondrial genome was amplified into three long overlapping fragments (from COI ~ ND4, ND5 ~ lrRNA, and lrRNA ~ COI) and subsequent several short fragments using the long fragments as temperate. The primers for both long and short fragments were designed solely for lepidopteran genomes, without any species-specific primers. As a usual the genome is composed of 37 genes: 13 protein-coding genes (PCGs), two rRNA genes, and 22 tRNA genes, and one large non-coding region termed the A+T-rich region. Arrangement of the genome is identical to those of other lepidopteran mitochondrial genome, but this differs from the common arrangement found in a diverse insect order, by the movement of tRNAMet to a position 5’- up stream of tRNAIle. Unlikely previous report on the start codon for COI gene in Lepidoptera S. cynthia ricini COI gene starts with typical ATT codon located between tRNATyr and the beginning region of COI gene. The 22 tRNAs that are interspersed throughout the mitogenome ranged in length from 62 to 71 bp. All tRNAs but tRNASer(AGN) were shown to be folded into the expected cloverleaf secondary structures. More detailed structural and phylogenetic analyses among Bombycidae and Saturniidae in connection with other families in the Bombycoidea will be performed soon

Field survey was conducted to determine the differences of spider community between traditional farming and environmental friendly farming at 17 localities, Korea in 2008. In guild structure, The occupancy rate of pest group, general group and natural enemy group was 37.3%, 41.0% and 21.6% respectively in environmental friendly farming, and 36.9%, 35.6% and 27.5% respectively in traditional farming. Among natural enemy group, parasitoids and predators were 15.8% and 84.2% respectively in environmental friendly farming, and 28.1% and 71.9% respectively in traditional farming. Among natural enemy group, rice field spiders were 74.4% and 62.2% in environmental friendly farming and traditional farming respectively. Among predators, rice field spiders were 86.7% in environmental friendly farming and 84.9% in traditional farming. Rice field spider community comprises 28 species of 24 genera belonging to 10 families and 30 species of 24 genera belonging to 10 families in environmental friendly farming and traditional farming respectively. Dominant families were Lycosidae, Linyphiidae and Tetragnathidae and dominant species were Pirata subpiraticus, Ummeliata insecticeps, Tetragnatha maxillosa, Gnathonarium dentatum and Packygnatha clercki in both agricultural practices. The ratio of hunting spiders and webbing spiders was similar between agricultural practices.

The devastating insect pests of the tomato (Lycopersicon esculentum) were surveyed in environmentally friendly (no-pesticide and organic) greenhouses located in the provinces of Gyeonggi, Gyeongsang, Gangwon, Chungcheong and Jeju. Whitefly, leaf miner, aphid and moth were recorded as the major pests in these surveys. Among them, whitefly was the most serious pest of greenhouse cultivated tomato and major species was Trialeurodes vaporariorum. The population densities of T. vaporariorum was 0~36 nymph per leaf. Population of moths Spodoptera litura were the highest in summer season. Population of the whitefly was considered to be influenced by the previous cultural methods. Farmers in organic cultivation, used insect natural enemy, commercial EFAM (Environment Friendly Agricultural Material), yellow sticky trap or home made pesticides. Commercial microbial pesticides were applied to evaluate their insecticidal activities against whitefly. The results revealed that microbial pesticides exhibited 65.3 to 100% whitefly nymph mortality. Further, pesticidal activities of Cooking Oil and Yolk (COY), plant extracts and neem oil were evaluated against other tomato pests as well.

The main nutritional problem of sorghum×sudangrass (sudangrass) silage is low quality and dry matter (DM) contents. This experiment was conducted to evaluate whether agricultural byproducts addition of sorghum×sudangrass silage increases DM content and forage quality of the silage. Sorghum×sudangrass silage with added byproducts had low pH values, however sorghum×sudangrass silage (control) had a high pH value because of its high moisture content. Silage added with byproduct had lower acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents than control silages, while its non-fiber carbohydrate (NFC), and total digestible nutrients (TDN) and lactic acid contents showed the opposite results. Silages added with crushed rice and green grain of rice were higher in lactic acid content than othor treatments as well as high DM, NFC, and TDN contents. Silage added with crushed rice and green grain of rice can be recommended as the most effective treatment for increasing forage quality and DM content of sorghum×sudangrass silage.

We present an optical-infrared photometric study of galaxies in six nearby clusters of galaxies at z=0.041∼0.098 (A1436, A1773, A1809, A2048, A2142, and A2152). Using BV I photometry obtained at the Bohyunsan Optical Astronomical Observatory and JHKS photometry extracted from the 2-Micron All-Sky Survey catalog, we investigate the colors of galaxies in the clusters. Using the (B − V ) versus (I −KS) color-color diagrams in comparison with the simple stellar population model, we estimate the ages and metallicities of bright early-type member galaxies. Early-type galaxies in each cluster show the color-magnitude relation. Ages and metallicities of early-type members show little dependence on their velocity dispersions. Mean ages of early-types in the clusters range from 3 Gyr to 20 Gyr, showing a large dispersion, and mean metallicities range from Z = 0.03 to 0.05 above the solar value, showing a negligible dispersion.

In this paper we develop an efficient procedure for solving the tactical-level room allocation problem for hotel industry by incorporating other sources of revenue into overall Revenue Management (RM) system. To maximize profits and allocate wisely available resources, first, determine how different market segments contributed. And apply this to an efficient procedure for solving tactical-level room allocation problem for the hotel industry by combining the dynamic model for handling single-night reservation requests and the static model for handling multi-night reservation requests.

Cloned calves derived from somatic cell nuclear transfer (SCNT) have been frequently lost by sudden death at 1 to 3 month following healthy birth. To address whether placental anomalies are responsible for the sudden death of cloned calves, we compared protein patterns of 2 placentae derived from SCNT of Korean Native calves died suddenly at two months after birth and those of 2 normal placentae obtained from AI fetuses. Placental proteins were separated using 2-Dimensional gel electrophoresis. Approximately 800 spots were detected in placental 2-D gel stained with coomassie-blue. Then, image analysis of Malanie III (Swiss Institute for Bioinformatics) was performed to detect variations in protein spots between normal and SCNT placentae. In the comparison of normal and SCNT samples, 8 spots were identified to be up-regulated proteins and 24 spots to be down-regulated proteins in SCNT placentae, among which proteins were high mobility group protein HMG1, apolipoprotein A-1 precursor, bactenecin 1, tropomyosin beta chain, H+-transporting ATPase, carbonic anhydrase II, peroxiredoxin 2, tyrosine-rich acidic matrix protein, serum albumin precursor and cathepsin D. These results suggested that the sudden death of cloned calves might be related to abnormal protein expression in placenta.

This paper is for applying interactive fuzzy linear programming for the problem of product mix planning, which is one of the aggregate planning problem. We developed a modified algorithm, which has two-phase approach for interactive fuzzy linear programming to get a better solution. Adding two-phase method, we expect to obtain not only the highest membership degree, but also a better utilization of each constrained resource.