목적 : 본 연구에서는 HMD VR을 사용해 발생한 사이버 멀미와 양안시 기능이 상관관계를 보이는지 알아보고 자 하였다. 방법 : 근거리 시력에 무리가 없는 20~40대 성인 52명을 대상으로 VR 콘텐츠 이용 전 양안시 기능을 측정하기 위해 수평사위도, 조절 근점, 폭주 근점, 양안조절용이성, 입체시, 상대조절력검사를 시행하였다. 광학 흐름 요소로 구분한 세 가지 VR 콘텐츠를 선정하여 대상자에게 사용하도록 하고 Virtual reality sickness questionnaire을 통해 VR 콘텐츠 이용 전과 후의 사이버 멀미를 측정하였다. 양안시 기능과 사이버 멀미에 대한 상관분석을 실시하 였다. 결과 : VR 콘텐츠 사용 후 콘텐츠 T와 R은 모든 항목에서 사이버 멀미(p<0.001)는 유의하게 증가하였다. 콘텐츠 B의 안구운동에서는 유의한 차이가 없었다. 상관분석에서는 근거리 사위가 콘텐츠 T의 방향감각상실(p< 0.050), 전체 VRSQ(p<0.050)와 음의 상관관계를 보였으며, 콘텐츠 R의 안구운동불편(p<0.050)과 유의한 상관관 계가 나타났다. 양안조절용이성은 콘텐츠 T의 전체 VRSQ(p<0.050)와 양의 상관관계를 보였다. 이 외에 다른 양안 시 기능은 사이버 멀미 요인과 유의한 상관관계를 보이지 않았다. 결론 : 양안시 기능은 사이버 멀미와의 결과로 사용자의 개인적 특성이나 양안시 기능 자체가 사이버 멀미에 미 치는 영향보다 3D를 구현하는 콘텐츠의 광학 흐름 요소가 미치는 영향이 더 높다는 것으로 사료된다. 향후 연구에 서는 VR 콘텐츠의 시각 요소와 시지각에 대한 요소가 고려되어야 할 필요성이 있는 것으로 사료된다.

The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

Extensive research and testing continue to be conducted for the development of vaccines targeting zoonotic diseases such as brucellosis. In this study, the potential of the DapB as a recombinant protein vaccine to effectively combat Brucella abortus 544 infection in BALB/c mice was evaluated. Western blotting assay results showed that recombinant protein DapB reacted with Brucella-positive serum, indicating its potential immunoreactivity. In vivo results showed that the peripheral blood CD4+ and CD8+ T cell population significantly increased in the DapB-immunized mice group after the first, second and third blood collection, compared to the control group that received PBS. Additionally, at the fourth blood collection, an increase in CD4+ T cell activation was observed in three vaccination groups compared to PBS negative control group. These results indicate the potential of DapB in stimulating cellular immunity. Fourteen days after infection, the bacterial load in the spleen was evaluated. The reduction in bacterial replication in the spleen by both DapB and RB51 highlights their protective efficacy against Brucella infection. These findings contribute to the ongoing efforts in developing effective vaccines against brucellosis and provide valuable insights for further research in this field.

Involving issues of environmental, consumer safety and health, and/or social concerns, a morally controversial action refers to an activity that does not have a single standard of ethics for all people, making it challenging to draw a clear line between what is ethical versus unethical. Despite its ambiguity, any firm can be involved in it, especially when the firm operates in the global marketplace where a variety of social, health, and environmental consequences surrounding a given action are questioned by consumers. If consumers come to know of a familiar brand’s morally controversial behavior, it can create approach-avoidance conflicts in their minds; they may see the brand’s action as mismatched with their own moral standards (“avoidance” manifested through a measurable construct––moral incongruence), while being tempted to justify the action (“approach” manifested through moral rationalization).

Subunit vaccines are being developed as a potential therapy for preventing microbial pathogen infection. In this study, the immunogenicity of recombinant Brucella (B.) abortus Fe/Mn superoxide dismutase (rFe/Mn SOD) protein as a subunit vaccine against B. abortus was investigated in BALB/c mice model. Brucella Fe/Mn SOD gene was cloned into a pcold-TF DNA vector. The bacterial recombinant protein was expressed using the Escherichia coli DH5α strain with a size of 82.50 kDa. The western blotting assay showed that rFe/Mn SOD reacted with Brucella-positive serum, indicating the potential immunoreactivity of this recombinant protein. After the second and third vaccinations, the peripheral CD4+ T cell population was increased significantly in the rFe/Mn SOD-immunized mice group compared to the PBS control group. Moreover, immunization of this recombinant protein increased the CD4+ T cell population from the first vaccination to the third vaccination. Meanwhile, the CD8+ T cells were slightly enhanced after the second vaccination compared to the first vaccination and compared to control groups. Fourteen days after the bacterial infection, the splenomegaly and the number of bacteria in the spleen were evaluated. The result showed that both rFe/Mn SOD and positive control RB51 decreased the bacterial replication in the spleen and the splenomegaly compared to control groups. Altogether, these results suggested that rFe/Mn SOD could induce host immunity against B. abortus infection.

MRI는 인체에 수소 밀도에 따른 재현성의 차이가 상대적으로 기존의 영상 장비들에 비교하여 큰 차이가 있으므로 임상 에서 이를 증명하고 문제 발견 시 이를 보완하는 것이 딥러닝 알고리즘은 매우 중요하다. 따라서 본 연구에서는 현재 특수 의료장비에서 권하는 미국 방사선 의학회(American College of Radiology, ACR)의 두부 전용 MRI 팬텀을 사용하여 영상 품질기준에 현재 임상 적용되고 있는 딥러닝 알고리즘 방법을 적용하여 딥러닝 알고리즘 적용 전후 변화를 평가해 보고자 하였다. 연구 결과 분해능을 측정하는 항목인 고대조도 공간 분해능과 같이 해상도와 관련된 영상 품질은 분해능은 개선되었음을 알 수 있었고, 그뿐만 아니라 위치의 정확도 역시도 기존에 딥러닝 알고리즘의 적용 전 영상과 통계적으로 차이가 있었다. 또한 딥러닝 알고리즘의 강도 차이에도 영상 간 차이는 없었다. 이러한 결과는 특수의료장비 영상품질관리 규정에 적용되고 있는 ACR 팬텀의 평가 기준에 부합 하나, 딥러닝 알고리즘 적용 전후 차이가 통계적으로 있었으며, 이러 한 차이가 재현성과 관련하여 추후에 조금 더 관련된 연구기 필요할 것으로 사료된다.

In operating or permanently shut down nuclear power plants which were built between 1970s and 1990s, asbestos was widely used for ceiling materials, wall materials, and gaskets. Furthermore, it was mainly treated as a heat-resistant material like insulation. In Kori Unit 1, radioactive asbestos was replaced or removed through maintenance and repair in the containment building during the operation period of about 40 years, but radioactive asbestos still remains that need to be partially dismantled. Generally, it is more difficult to handle because it belongs to two different waste categories, radioactive waste and hazardous waste. In addition, the risk increases further due to radioactivity with the asbestos hazards itself. Therefore, it is very important to accurately determine the amount of radioactive asbestos waste and to evaluate the treatment method and disposal reduction rate before the decommissioning is started. According to the Korean Waste Management Act, three methods are recommended for the asbestos (hazardous waste) treatment: landfill, solidification, and high-temperature melting. Landfill is commonly used in Korea and the United States while high-temperature melting and solidification are additionally recommended only in Korea. Considering the situation in Korea, landfill is not appropriate due to the limitations of landfill capacity and potential risks (hazards still remain). Therefore, the other two methods can be considered sufficiently in terms of safety, detoxification, and reduction rate. This paper evaluates the amount of radioactive asbestos waste at Kori Unit 1 based on the actual asbestos building material data (as of February 2022) of the Asbestos Management Comprehensive Information Network. Vitrification is considered as a sufficient alternative for treating radioactive asbestos waste. And, it is checked whether the vitrified waste through the high-temperature melting method, plasma torch, meets the requirements such as detoxification, compressive strength and leachability for storage and disposal stability. It is expected to be useful to prepare a radioactive mixed waste management standard and to reduce the disposal cost through the reduction of final waste.

Nuclear power plants, like other national critical infrastructures, could be under the threat of terrorism or other malicious action. Thus, a nuclear power plant has a robust security system that includes security guards, sensors, barriers, access control systems, lights, and alarm stations with security procedures. However, an effective security system is hard to design because a chain is only as strong as its weakest link, and there could be a vulnerable hole even in the robust security system. Thus, an effective security system requires the evaluation of all possible scenarios. Evaluation software for security system effectiveness assists in systematically assessing all the possible attack scenarios. Many countries developed security effectiveness evaluation software. The first software was developed by the U.S. Sandia National Laboratories in the 1980s. Now there are several commercially available software packages with a function to simulate limited-scope combat between security guards and attacking enemies. However, academic communication is comparatively weak because it may contain sensitive information on the vulnerability of nuclear power plants. We developed original software called Tools for Evaluating Security Systems (TESS) to identify the most vulnerable path to the designated target and model the security systems of all South Korean nuclear power plants. We also used commercial security effectiveness evaluation software, AVERT, to model the same nuclear power plants. TESS was developed to verify the results of commercial security effectiveness evaluation software for the purpose of regulatory use. For the feasibility test, we compared the results of two software with those of force-on-force (FoF) exercises in nuclear power plants. According to the relevant Act, every nuclear power plant site should perform the FoF exercises every year. KINAC was in charge of evaluating the FoF exercise and used several of its results for the study. In the results, even in some differences in detail, the two software and FoF exercises showed qualitative similarity. Conclusively, evaluation software is a useful tool to design and/or assess the security systems of nuclear power plants. We modeled the security systems of all South Korean nuclear power plants, and compared the developed software, a commercial software and FoF exercises. The results showed qualitative similarity. We provided the results of evaluation to nuclear operators for the better security of nuclear power plants.

Chlorine dioxide (ClO2) has recently emerged as an ideal disinfectant and has shown a wide range of antimicrobial activities in various pathogenic microorganisms. In this study, the virucidal effect of ClO2 at low concentration (0.02 ppm) and higher concentration (0.06 – 0.09 ppm) against Adenovirus and Herpesvirus was evaluated based on the NF T 72-281 and ASTM 1053-11 standard methods at different exposure times. The virus suspension was dried onto the carrier and then exposed to gaseous ClO2 (gClO2) at 22 ± 2∘C. For Adenovirus, exposure at a low concentration of ClO2 at the middle height resulted in the average log10 reduction of 0.95, 2.65, and 5.30 after 1, 3, and 6 h post-exposure (pe), respectively. Moreover, more than 4-log10 reduction was achieved at 4 and 6 h pe with higher concentrations of ClO2. On the other hand, the antiviral activity of gClO2 at the middle height was also effective against Herpesvirus. In particular, at 1 h pe, a less than 4-log10 reduction was observed at all examined concentrations of ClO2, whereas exposure for 3 and 6 h (with low concentration) or 2 h (with higher concentration) inactivated completely viruses attached to the carrier. These results suggested that ClO2 fumigation is a potential alternative method for disinfecting healthcare facilities, high-containment laboratories, and households with a safe concentration for human health.

The purpose of this paper was to introduce positive psychology, to the fields of SLA and English education in Korea. Positive psychology investigates how people flourish and seeks the virtues and strengths of humans. It focuses on the factors enabling people and their communities to thrive, instead of focusing on psychological disorders and abnormalities, longstanding issues of general psychology. Its three main research topics include positive emotions, traits, and institutions, all of which have relevance to SLA and L2 education. This paper examined how these topics have been approached in SLA. Much attention has focused on positive emotions, particularly, enjoyment, which has gained momentum in recent years with publications. Empirical studies of the impacts of positive emotions on L2 learning were reviewed with discussion of enjoyment. The paper concludes by discussing directions for future research, applying positive psychology to Korean EFL contexts.

We investigated the effect of a synthetic complement peptide C3a on the outcome of Brucella abortus 544 infection in a murine macrophage cell line RAW264.7 cell. First, we determined the highest non-cytotoxic concentration of the peptide in the cell line. We also found that the peptide significantly increased the growth of the bacteria at 8 and 24 h. Although the number of bacterial CFU was also elevated at 48 and 72 h, the increases were not significant as compared to controls. We further investigated the effect of C3a peptide on the growth of Brucella by pre-incubating the peptide at various temperatures and found that the effect was reversed at 24 h post-incubation suggesting that incubation of peptide at high temperatures including 65°C or 95°C could inactivate its action. This also could indicate the beneficial effect of high temperature during infection. Although several studies reported the inhibitory effect of different antimicrobial peptides including C3a, the present study preliminarily revealed that it had no positive contribution on the control of B. abortus 544 infection in vitro and indirectly to its receptor, CD88, which belongs to GPCR. Moreover, the encouraged further exploration of the effect of other similar peptides would be performed for the purpose of finding Brucella-host cell interaction for the control of disease progression.