본 연구는 제주지역에서 초식동물의 방목을 위해 주로 이용되고 있는 한지형 목초의 경우에 여름철 하고기에 생산성이 저하되고 있어 여름철에 생산성을 높일 수 있는 난지형 목초를 도입하여 이용하기 위한 목적으로 수행되었다. 시험에 사용된 난지형목초는 Bermudagrass 품종(Cynodon dactylon (L.) Pers. : Giant, Cheyenne, Mohawk, Panchero Frio, Common, Tifton 85)과 Bahiagrass 품종(Paspalum notatum : TifQuik, Tifton 9)을 이용하였으며, 해발 450m고지에서 월동 후에 수확시기별 생산성 및 영양소 함량의 변화를 조사하였다. 시험구는 품종별로 8처리 3반복 난괴법으로 배치하여 수행하였다. 파종방법은 산파와 조파형태로 파종되었으며, Tifton 85 품종은 줄기 파종되었다. 시험결과를 보면 월동 후에 Bermudagrass와 Bahiagrass 품종 모두에서 동해에 의한 피해로 봄철에 재생이 대체로 늦었으며, 이중 줄기 파종되었던 Bermudagrass의 Tifton 85와 TifQuik, Tifton 9 품종이 다른 품종에 비해 동해에 비교적 강한 것으로 평가되었다. 생초와 건물수량은 품종에 따라 유의성 있는 차이를 보여 주었는데(p<0.05). Tifton 85, TifQuik 및 Tifton 9 품종의 생초 수량이 다른 품종에 비하여 우수하였으며, 건물수량은 Tifton 85와 Tifton 9 품종이 다른 품종에 비하여 우수하였다. 건물 함량에 있어서도 품종에 따라서 유의있는 차이를 보여주었고(p<0.05), Giant 품종이 다른 품종에 비하여 높은 결과를 나타내었다. 수확시기별 건물 함량은 Bermudagrass의 모든 품종에서 수확시기에 따른 차이를 보였다(p<0.05). 조단백질 함량도 품종에 따라 유의있는 차이를 나타내었고(p<0.05), Cheyenne, Mohawk, Panchero Frio와 Common 품종이 다른 품종에 비하여 높은 결과를 보여주었으며, Giant 품종에서는 파종방법과 수확시기에 따른 차이를 나타내었다(p<0.05). 조섬유 함량도 품종에 따라 차이를 나타내었으며(p<0.05), Tifton9 품종이 다른 품종에 비하여 유의있게 높은 함량을 보여주었고, 수확시기별 조섬유 함량은 Mohawk과 Tifton 85 품종에서 유의있는 결과를 나타내었다(p<0.05). 그리고 조회분 함량에 있어서도 품종에 따른 유의있는 차이를 보였으며(p<0.05), Cheyenne이 다른 품종에 비하여 유의있게 높은 함량을 보였다. 수확시기별 조회분 함량은 Cheyenne, Panchero Frio와 Tifton 85 품종에서 유의있는 결과를 나타내었고(p<0.05), 파종방법에 따른 차이는 Giant 품종에서 유의있는 결과를 보였다(p<0.05). 이러한 결과에 따르면 난지형 목초류는 품종에 따라 동해에 따른 재생능력, 생산성, 영양소 함량이 차이가 크게 나타나고 있어 이용목적에 따라 선택할 필요가 있으며, 이용을 확대하기 위하여서는 다양한 해발고도에서의 평가가 필요한 것으로 사료되었다.

The objective of this study was to determine the growth characteristics of cool (C1) and warm season grasses (C2) in pastures mixed with C1 and C2 at an altitude of 400 m in Jeju island to establishing pasture suitable for grazing horses and to evaluate the effect of pastures mixed with tall and short type grasses on the intake characteristics of horses. C1 used in this study was Kentucky bluegrass, redtop (short type grass) and tall type grasses were orchardgrass and tall fescue, respectively. Treatments of this study were consisted of four groups and the short type grass used in pastures mixed with C1 and C2 was mainly bermudagrass. Four treatment groups were follow as; Treatment 1 (bermudagrass + Kentucky bluegrass + redtop) 2) Treatment 2 (bermudagrass + tall fescue + orchardgrass) 3) Treatment 3 (Kentucky bluegrass + redtop) 4) Treatment 4 (tall fescue + orchardgrass). Bermudagrass was a little winter killing and inhibition of plant growth at an altitude of 400 m. Plant heights in pastures mixed with C1 and C2 were grown better than that in pastures mixed with C1. Especially, plant height in Treatment 4 was higher than other treatments. Dry matter yield was in the following order: Treatment 4> Treatment 3> Treatment 2> Treatment 1. Dry matter yield in pastures mixed with C1 increased as compared with pastures mixed with C1 and C2. Dry matter yield in Treatment 3 was higher than other treatments. In the first investigation regarding vegetation distribution, bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 11.7 and 13.3%, respectively. The growth of bermudagrass in winter was low due to the cold damage. However the growth of Kentucky bluegrass, redtop, tall fescue and orchardgrass was good. In the second investigation, bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 5.0 and 11.7%, respectively. Growth of forage in the second investigation was poor as compared to the first investigation. nutritive values(crude protein content, neutral detergent fiber content, acid detergent fiber content, digestibility) were good in pastures mixed with C1 Especially, nutritive values in pastures mixed with tall was higher than those of pastures mixed short grasses. P content among minerals in Treatment 1 was higher than other groups. However, the content of Ca, Mg and Mn were lower. The contents of Ca, K, Mg, Na, Cu, Zn and Fe in Treatment 2 were higher. However, the contents of K, Mg, Na, Cu, Zn and Fe in Treatment 3 were lower. Therefore, we suggest that cool season grasses with short grasses were sowed to establishing pasture suitable for grazing horses at an altitude of 400 m in Jeju island.

본 연구는 2014년 7월부터 2015년 9월까지 제주 한국마 사회 제주육성목장(해발 450m) 시험포장에서 여름 동안 생산성이 유지될 수 있는 남방형 목초를 도입하여 재배에 이용하기 위하여 Bermudagrass 품종(Cynodon dactylon (L.) Pers. : Giant, Cheyene, Mohawk, Panchero Frio, Common) 과 Bahiagrass 품종(Paspalum notatum : TifQuik, Tifton 9, Argentine), 그리고 Teff grass(Eragrostis tef : Tiffany) 품종 의 생육특성 및 건물 수량을 평가하기 위하여 실시하였다. 실험설계는 품종을 처리로 10처리 3반복 난괴법으로 배치 하였다. 1차년도 1차 수확은 8월 31일, 2차 수확은 9월 21일이며 2차년도 1차 수확은 7월 22일, 2차 수확은 8월 14 일, 3차 수확은 9월 11일로 성장단계를 고려하여 수확하였 다. 1차년도 남방형 품종의 건물 수량은 Teff grass의 Tiffany가 산파와 조파 각각에서 2.59와 2.00Ton/ha로 가 장 높은 건물수량을 나타내었으며, Bermudagrass의 Common이 산파에서 1.21Ton/ha로 다년생 남방형 목초 중 가장 높은 수량을 나타내었다. 2차년도 남방형 품종의 건물 수량은 Bahiagrass의 Tifton9이 산파에서 15.06Ton/ ha로 가장 높은 수량을 나타내었으며 Bahiagrass의 Tifquik이 산파에서 14.68Ton/ha로 두 번째로 높은 수량을 나타내었다. 조단백질 함량은 Bahiagrass의 Argentine이 19.75%로 가장 높게 나타났으며 NDF 함량은 Bermudagrass 의 common이 61.69%로 가장 높은 것으로 나타났다. 한해 에 의한 피해의 경우 대부분의 Bermudagrass 품종의 경우 에 동절기 적응성이 떨어졌으나 Bahiagrass의 Tifton 9 품 종의 경우 한해에 대한 피해를 거의 받지 않았으며 출현, 내병성, 내습성, 도복성은 품종 간 차이가 거의 없었으며 Teff grass의 Tiffany이 도복과 습해에 의한 피해가 나타났다. 이상의 결과를 종합해 볼 때 제주지역에서 Bermudagrass와 Bahiagrass의 일부 품종은 월동할 수 있어 여름철 조사료자 원으로 활용이 가능하지만, 품종 간 내한성 능력과 재생능 력이 다르므로 이를 고려하여 품종을 선택할 필요가 있다.

본 연구는 농후사료의 급여수준을 달리하여 제주산마 에게 급여하는 경우에 체중 변화, 장내 미생물 조성 및 혈액성상의 변화에 미치는 효과를 구명하기 위하여 수행 되었다. 공시축으로 24-36개월의 제주산마(제주마×더러브 렛) 14두를 공시하였으며, 농후사료의 급여수준을 체중의 1.5%(5두), 2%(4두)와 2.5%(5두)로 급여하여 봄과 겨울 증 체율 및 혈액 특성과 생리적 변화를 조사하였다. 봄에 총 증체량은 1.5%, 2%, 2.5% 급여한 처리구에서 12.6kg, 21.5kg, 29.0kg, 겨울에 총 증체량은 21.6kg, 28.0kg, 42.6kg을 나타냈고, 일당 증체량은 봄에는 0.23kg, 0.39kg, 0.54kg, 겨울에는 0.40kg, 0.52kg, 0.79kg을 나타내어 체중의 2.5%를 섭취한 구에서 높은 경향을 나타내었다. 내독 소는 농후사료 과다 섭취에 의해 그람음성균의 세포벽에 서 방출되는 물질로 봄에 시행한 시험에서 3주차 때 농 후사료를 체중의 2%와 2.5%를 급여한 처리구에서 유의 적으로 낮은 결과를 나타내었으나(p<0.05) 이후에는 점차 모든 처리구에서 수치가 비슷해져 적응하는 경향을 보여 모든 처리구가 농후사료 급여에 적응하는 경향을 보였다. 봄에 실시한 실험에서 8주차에 분 pH는 농후사료를 체 중의 1.5%, 2%, 2.5% 급여한 처리구에서 각각 6.2, 5.8, 5.7로 나타나 농후사료를 체중의 2%와 2.5%를 급여한 처 리구에서 유의적으로 낮은 결과를 나타내었다(p<0.05). 직장온도는 겨울에 실시한 실험에서 8주차에 농후사료를 1.5%, 2%, 2.5%. 급여구에서 각각 37.8℃, 37.9℃, 38.4℃ 를 나타내어 농후사료 2.5% 급여구에서 직장온도가 유의 적으로 높은 수치를 나타내었다(p<0.05). 분내 미생물에 서는 셀룰로오스와 탄수화물(구조 탄수화물) 분해에 관여 하는 미생물로 알려진 Fibrobacter가 체중의 1.5% 급여했 을 경우 총 미생물군에서 2.49%를 차지하였고, 2%와 2.5% 급여구에서는 각각 1.42%와 1.09%를 차지하여 농후 사료 급여량이 증가할수록 유의적으로 감소하는 경향을 보였다. 결론적으로 농후사료 2.5%의 급여가 비육마 사육 방법으로 활용할 수 있을 것으로 사료된다.

The objective of this study was to determine the growth characteristics of cool (C1) and warm season grasses (C2) in pastures mixed with C1 and C2 suitable for grazing horses and effect of pastures mixed with tall and short grasses on the intake characteristics of horses. C1 used in this study was Kentucky bluegrass, Redtop (short type grass) and tall type grasses were orchardgrass and tall fescue, respectively. The short type grass used as C2 was Bermudagrass. This study had the following four treatment groups: 1) Treatment 1 (Bermudagrass + Kentucky bluegrass + Redtop) 2) Treatment 2 (Bermudagrass + tall fescue + orchardgrass) 3) Treatment 3 (Kentucky bluegrass + Redtop) 4) Treatment 4 (tall fescue + orchardgrass). There was no winterkilling or lodging problem at an altitude of 200 m. Plant heights in mixed pasture of Treatment 1, 2, 3, and 4 were 53.9, 58.2, 57.5, and 78.1 cm, respectively. Plant height was the highest in Treatment 4. Dry matter yield was in the following order : Treatment 4 > Treatment 3 > Treatment 2 > Treatment 1. In the first investigation regarding vegetation distribution, Bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 70% and 66.7%, respectively. Overall, other grasses showed poor growth. In the second investigation of vegetation distribution, Bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 80% and 60.7%, respectively. Crude protein content, neutral detergent fiber content, acid detergent fiber content, digestibility value, and nutritive values were the lowest in Treatment 4, followed by those in Treatment 1, Treatment 2 and Treatment 3.

본 연구는 오차드그라스와 톨 페스큐 혼파초지에서 비축 시기별(8월 1일, 15일, 30일) 으로 처리하여 11월 중순경(11.10∼11.12)에 수확할 때 목초의 비축시기에 따른 생산량과 목초의 사료 가치 등을 비교하기 위해 수행하였다. 목초 비축시기에 따른 오차드그라스와 톨 페스큐 혼파초지(동량 혼파초지)의 건물수량은 8월하순구가 2,983.0kg/ha으로 가장 높은 경향을 보였다. 목초의 비축시기가 늦어짐에 따라 목초의 생산량도 증가하였으나 유의성이 인정되지는 않았다. 조사료의 조단백질은 16.5∼18.16%으로 목초 비축시기별로 일정한 경향을 나타내지 않았다. NDF와 TDN는 비축시기가 늦을수록 증가하는 경향을 나타내었으나 유의성이 인정되지는 않았다. 반면에 ADF 비율은 목초 비축시기가 감소함에 따라 점차 감소하는 것으로 나타내었으나 유의성이 인정되지는 않았다. 무기물의 변화는 비축 기간이 증가할수록 P, Ca, Mg, Na, Zn이 증가하였고 반면에 Mn, Cu는 감소하는 경향을 나타내었다. 결과적으로 8월 초순부터 초지를 비축할 경우 고온으로 인한 생육 위축으로 목초의 수량증가는 기대하기 어렵다. 따라서 제주도에서 가을철에 활용하기 위한 초지를 비축할 경우에는 8월 하순 이후에 시작하는 것이 바람직할 것으로 사료된다.

We investigated the effect of glutathione supplementation on feed intake, body weight loss, behavior change and economic analysis of elk bull in breeding season. Sixteen elk bulls (5-year-old, average weight; 330 kg) after antler cutting were divided into 2 groups. Eight bulls in each group (control and glutathione group) were fed experimental diet at a level of 0.85 percent of body weight and 6 mg glutathione per kg body weight. As a result, weight-loss of control animal during experimental study (from September to November) was 42.6±19.2 kg while that of glutathione-supplemented group was 20.6 ±19.9 kg. Compared to control group, glutathione-fed group had prevented the body weight loss by 5.2% (p<0.05). Although the feed intake of elk voluntarily decreases during breeding season, daily DM intake per head was 5.59 and 5.80kg in control and glutathione-fed group, respectively. While the statistical difference in feed intake between two groups was not observed, feed intake tended to increase in glutathione-fed group. In economic analysis, additional cost of 99,000 KRW per head was spent due to the cost of glutathione because of its import. Changes in behavior such as crying, deer whistles, whistling intensity and frequency of accident were lower in glutathione-fed group compared to control. During breeding season, use of glutathione as feed supplement could suppress body weight loss and accidents in deer farm.

The objective of this study was to compare the accuracy analysis and the effect of field application of a newly developed automatic heat detector in dairy cows. From 2009 to 2010, we used 48 Holstein cows (mounting cows : 38 heads, standing cows : 10 heads) raised in experimental barn of National Livestock Research Institute (RDA) for the accuracy analysis of automatic heat detector, and 14 Holstein cows raised in three commercial dairy farms of Cheonan and Pochun area for comparison of the effect of field application. The accuracy of response in cows attached with automatic heat detector was 86.8% (33/38) displayed on board when mounting activity observed, and 100% (10/10) when standing activity observed, and on average, 90.0% (43/48) displayed on board. The accuracy of automatic heat detector in on-farm test was 85.7% (12/14), and conception rate was 75.0% (9/12).

We investigated the effect of glutathione supplementation on body weight gain, feed intake, velvet antler yield and economic analysis in elk bulls. A total 14, 2-year old male elks were divided into 2 groups with control or glutathione treatment. Elks were fed concentrate feed at the level of 1.5% relative to body weight (3.1 kg). and allowed to consumed hay as roughage ad libitum. Glutathione was supplemented at the level of 6 mg/kg. Average daily gains (ADG) for 2-years old elks were 234.1± 7 and 247.6±22 kg in control and glutathione fed groups, respectively. Treated group had higher ADG than control (p<0.05). Individual daily DM intakes were 5.34±0.70 and 5.64±0.71 kg in control and glutathione supplemented groups, respectively. Glutathione-fed group showed an additional intake of 298 g on an average. Production of velvet antlers for elk yearlings was 4,229±720 g and 4,653±960 g in control and glutathione supplemented groups respectively. Analysis of economics efficiency revealed 8% higher revenue index in glutathione supplemented groups. In conclusion, glutathione supplementation showed increase of DM intake and ADG in elk bulls, and could also increase velvet antler production.

We investigated the effect of glutathione supplementation on feed intake, body weight gain, velvet antler yields and economics of elk yearling. Fourteen elk yearlings were divided into 2 groups. Seven yearlings in each group (control and treatment) were fed with 1.5-2.5 percent body weight (%BW) of concentrate feed for elk, voluntary intake of hay as roughage and 6 mg/kg body weight (KBW) of glutathione. The results were as follows. Average daily gain (ADG) for control elk yearlings for first 1.5 month was 0.46 kg, while that of glutathione supplemented was 0.55 kg. Although glutathione fed group had higher ADG compared to control group (p<0.05), ADG after 1.5 month showed no difference. In spring, daily DM intake per elk yearlings was 3.98 and 4.24 kg for control and glutathione supplemented groups, respectively. The statistical differences in feed intake between two groups were not observed. However, feed intake tends to increase in glutathione fed group. Production of velvet antlers for elk yearlings were 911±256 and 1066±357 g for control and glutathione supplemented groups, respectively. Statistical difference between two groups was not observed due to the high variation. In economic analysis, additional 109,110 KRW per head for the cost of glutathione resulted in 2 percent higher revenue index due to the increased antler production. In conclusion, feeding glutathione to elk deers effectively increased DM intake and ADG of elk yearlings. Glutathione supplementation in feed might increase velvet antler production as well as ADG.

Substantial efforts have been made to manipulate ruminal environment in a hope to enhance ruminal fermentation efficiency for better ruminant productivity. Some of examples are methane inhibitors, antibiotics, microbial enzymes, fatty acids and/or lipid feeding, buffering agents, ionophores and probiotics. Of these efforts, the non-ionic surfactant (NIS) has been known for its stimulation to release enzymes from a range of anaerobic microbes. A series of studies were conducted 1) to evaluate NIS diluted with water and ethanol on in vitro ruminal fermentation and 2) to determine the influence of diluted NIS on digestibility of feedstuffs. In first experiment (Exp. 1), NIS was diluted with water or ethanol to measure its effects on in vitro microbial growth, ruminal enzyme activities and gas production by mixed ruminal microbial culture. The NIS was diluted with water or ethanol separately in a 1:5 ratio (w/v). Water and ethanol-diluted NIS with wheat flour were added with rice straw-based mixed ruminal microbial cultures at the rate of 2 ㎎ NIS/16 ㎖ McDougall buffer plus 4 ml ruminal fluid solution. The mixed ruminal microbial culture was run without any NIS addition as control. Addition of NIS either diluted with water or ethanol has significantly reduced the gas production in mixed ruminal microbial culture at 12 and 24 h of incubation. At 48 h post incubation, gas production was the highest with the addition of ethanol diluted NIS followed by water-diluted NIS and control. Carboxy methyl cellulase activity in rice straw-based mixed ruminal bacterial culture was significantly higher with the addition of ethanol-diluted NIS compared with the addition of water-diluted NIS and control at 24 and 72 h post incubation. In second in vitro experiment (Exp. 2), effects of addition of ethanol diluted NIS on dry matter (DM) digestibility of alfalfa hay, gas production, pH and cellular growth in mixed ruminal microbial culture were examined. Alfalfa hay based mixed ruminal microbial culture without any NIS addition was run as a control. The pH of mixed ruminal microbial culture was significantly lower than control at all post incubation sampling hours. In vitro DM digestibility of alfalfa hay was significantly higher with the addition of NIS compared with control. Gas production was significantly less with NIS addition compared with control at all post incubation sampling hours. Microbial growth in mixed ruminal microbial culture was significantly increased with the addition of NIS compared to control.

유산양의 에너지 및 단백질 이용성을 조사하기 위하여 대조구(A), TDN 증량구(B), 조단백질 증량구(C) 그리고 TDN 및 조단백질 증량구(D)로 구분하여 섭취량, 소화율과 질소 및 에너지 이용성을 시험하였다. 공시축은 유산양(Saanen, 평균체중 17.5kg, female) 12두를 공시하였고, 2008년 10월 20일부터 11월 9일까지 충남대학교 부설동물사육장에서 수행하였다. 건물 섭취량은 D구에서 가장 높았으며 B구가 C구보다 유의적으로 높