본 논문에서는 각 사용자에게 적합한 게임의 난이도 조절을 위하여 인간의 생리적 반응을 분석하는 방법을 이용하여 객관적이고 정량적인 사용자 반응을 측정하는 방식을 사용하였다. 생리적 신호 측정 방식 중 인간의 인지와 감정을 계량하는데 적합한 뇌파 신호를 측정하고 기존의 연구에서 사용되었던 지표를 이용해 측정한 신호를 분석하였다. 그리고 기존의 다른 분야에서 사용하던 지표를 게임의 난이도에 적합하게 개선하여 기존의 방식을 그대로 사용한 결과와 비교하여 어떤 개선점을 보이는지 확인하였다. 이를 위해 먼저 피험자의 휴식 상 태에서 나타나는 뇌파를 측정하여 기준이 되는 배경 뇌파로 사용하였다. 테트리스에 두 가지 난이도를 설정하고 각각의 난이도를 진행하는 피험자의 뇌파를 측정하였다. 각 난이도에서 측정한 뇌파와 기준이 되는 배경 뇌파의 차이를 각 난이도를 진행할 때 피험자가 보이는 뇌파 반응의 결과로 간주하였다. 이 결과를 기반으로 지금까지 다양한 분야에서 지표로 삼았던 뇌파의 대역별 주파수 변환 결과를 비교 지표로 사용하는 방식과 좌뇌와 우뇌의 비대칭성을 기반으로 긍정, 부정을 파악하는 지표로 사용하는 방식, 알파파와 베타파의 비례 값을 각성, 이완의 지표로 사용하는 방식으로 분석하였다. 마지막으로 세타파를 기준으로 알파파와의 비례 결과와 베타파와의 비례 결과를 각각 몰입과 긴장의 지표로 이용하여 분석하였다.
Esophageal cancer is a representative cancer that occur physical deterioration but, physical problems after surgery were not well reported. The purpose of this study is to report on the long thoracic neuropathy after surgery, and to identify the symptoms and effects of physical therapy after esophageal cancer surgery. This is a case of a 61-year-old man who showed winging of the scapula with long thoracic nerve injury on the results of electromyography after an esophageal cancer surgery. Physical therapy programs were implemented 8 sessions during hospitalization. The quality of life, fatigue, shoulder range of motion (ROM), numeric rating scale (NRS), 6-minute walk test, and 30-second chair stand test were assessed. The quality of life, fatigue, shoulder ROM, NRS (pain), 6-minute walk test, and 30-second chair stand test were improved. However, the esophageal-specific symptom was not different after physical therapy program. As esophageal cancer suffers from physical difficulties after surgery, physical therapy programs are thought to be helpful.
Artificial insemination is a technique to transfer instrumentally sperm from the male into the female’s reproductive system. A key factor among artificial insemination techniques is gathering sperms. Here, a method for collecting sperm of bumblebee male by pressing was firstly developed. This method has stage of separating reprodutive tract of male, separating accessory testis, separating vas deferens, pressing vas deferens with cover-glass and collecting sperm with syringe. The developed method was 2.8 fold higher in rate of collecting sperm than that of existing method. Among 1M NaCl-, Insect ringer solution-, Phosphate buffered saline(PBS)-collecting sperm solutions, PBS was exhibited the best result as 17.2 sperms/cell, which corresponded to 1.6-1.8 fold increased over the result of other collecting sperm solution. Consequently, we think that this method is very important technique to save time for collecting sperm, to keep activity of sperm and to reduce contamination of sperm.
The anti-proliferative efficacy of t,t-conjugated linoleic acid (t,t-CLA), c9,t11-CLA, and t10,c12-CLA was compared in several human cancer cell lines. Gastric NCI-N87, liver Hep3B, pancreas Capan-2, and lung NCI-H522 cancer cells were incubated with 50 μM CLA isomers over a period of 6 days. The t,t-CLA inhibited the growth of all cancer cell lines to different extents, but c9,t11-CLA and t10,c12-CLA inhibited or stimulated the growth of the cancer cell lines. NCI-N87 cells were the most sensitive to growth inhibition and apoptosis from all CLA isomers tested. In NCI-N87 cells, CLA isomers reduced the release of arachidonic acid (AA) via the inhibition of cytosolic phospholipase A2 (cPLA2 ) activity, consequently reducing the production of PGE2 through the inhibition of cyclooxygenase-2 (COX-2). The efficacies of CLA isomers were in the following order (from most to least effective): t,t-CLA, t10,c12-CLA and c9,t11-CLA. Overall, these results imply that the anti-proliferative efficacy of t,t-CLA on cancer cells, especially NCI-N87 cells, was greater than other CLA isomers due to its induction of apoptosis through the inhibition of cPLA2 and COX-2 activities.
Gold kiwifruit was fermented with Lactobacillus plantarum CK10 derived from kimchi and the fermented products were extracted with ethanol at various fermentation time-points The bacterial cellular density, total titratable acidity, total polyphenol content (TPC), and total flavonoid content (TFC) increased during fermentation, while pH values and total soluble solids decreased. Levels of TPC and TFC were highest after five days, at 1.21±0.13 mg GAE/g dry weight and 0.36±0.04 mg RE/g dry weight, respectively. The antioxidant activities of the fermented gold kiwifruit were analyzed using Fe2+ chelating activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH)-, and 2,2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS)- radical scavenging activities, and superoxide dismutase (SOD)-like activity. The Fe2+ chelating activity of gold kiwifruit (125-500 μg/mL) peaked after five days of fermentation at 38.40-78.47%. The DPPH radical- scavenging activity and SOD-like activity were somewhat higher after seven days of fermentation (36.01-86.81% and 54.79-93.83% at 2.5-10.0 mg/mL concentration of samples, respectively). On the other hand, the ABTS radical- scavenging activity of fermented gold kiwifruit was similar to that of the non-fermented form. The polyphenol and flavonoid contents were significantly correlated with the antioxidant activity. In conclusion, our results suggest that TPC, TFC, and antioxidant activity were increased after five and seven days of fermentation, respectively. Therefore, fermented gold kiwifruit with its increased antioxidant activity could be useful in the development of functional foods.
A new pink gerbera (Gerbera hybrida Hort.) cultivar “Pink Giant” was developed from a cross between “Salina” and “Marita” followed by seedling and line selections at the Flower Breeding Research Institute, Gyeongnam Agricultural Research and Extention Services (ARES) in 1999. Characteristic trials were conducted three times from 2002 to 2004. Newly bred “Pink Giant” have shown pink color (RHS, 65-B), semi-double type, and large flower. It also was in good harmony in ray floret color and flower center color with stable flower shape. The vase life was 19.2 days. The average yield of “Pink Giant” was 45.8 flowers per plant in an year in greenhouse yield trails during 2003 and 2004. This cultivar was registered for a commercialization in 2005. “Pink Giant” would be cultured under greenhouse condition in Korea.
A new yellow gerbera (Gerbera hybrida Hort.) cultivar “Gold Eye” was developed from a cross between “Songsongee” and “White Day” followed by seedling and line selections at the Flower Breeding Research Institute, Gyeongnam Agricultural Research and Extention Services (ARES) in 2001. Characteristic trials were conducted three times from 2003 to 2004. Newly bred “Gold Eye” have shown yellow color (RHS, 55-C), semi-double type, and large flower. It also was in good harmony in ray floret color and flower center color with stable flower shape. The vase life was 10.2 days. The average yield of “Gold Eye” was 48.8 flowers per plant in an year in greenhouse yield trails during 2003 and 2004. This cultivar was registered for a commercialization in 2005. “Gold Eye” would be cultured under greenhouse condition in Korea.
“Woncheongbyeo” is a new japonica rice (Oryza sativa L.) cultivar developed by mutation techniques from Chu-cheongbyeo. Chucheongbyeo seeds were irradiated with 300 Gy gama ray (83.3 rad/min) emitted from 60Co at a radiation facil-ity of the Korea Atomic