The study aims to assess viability, acrosomal menbrane, integrity and mitochondria membrane potential of sperm separated using a percoll density gradient(45% and 90%) and swim-up methods using Hanwoo epididymal sperm frozen semen. Briefy, motile sperm separated using a percoll gradient and swim-up. 25 μl of sperm dilution from droplets were transferred to 1.5 mL tube and incubated with fluorescent probes at 39°C in dark as follows. After incubation, 75 μl of 5,5',6,6'-tetrachloro-1,1',3,3'- -JC-1; Mitochondrial Membrane Potential Detection kit, Cell Technology Inc., USA) working solution was mixed with sperm dilution and incubated for 30 min. One μl of Hoechst 33342 (H1339; Molecular Probe, Eugene, OR, USA) stock solution was mixed with sperm dilution and incubated for 10 min. And then, 0.5 μl of propidium iodide (PI) stock solution and 0.5 μl of fluorescein peanut agglutinin FITC conjugate (FITC-PNA; Vector Laboratories, FL-1071) were mixed with sperm dilution and incubated for 8 min. After mixing with fluorescent probes and sperm dilution, 5 μl of stained sperm dilution was mounted on a slide glass and covered with cover glass. More than 200 sperms in a slide glass were counted with × 400 magnification by fluorescent microscope (Eclipse Ci_L, Nikon, JAPAN) and evaluated viability, acrosomal membrane integrity, and mitochondrial membrane potential of sperm with triple band filter (DAPI/FITC/TRITC; Nikon, Tokyo, Japan). Live sperm were stained with Hoechst 33342(blue) and dead sperm were stained with PI (red). Damaged acrosomal membrane of sperm was stained with FITC-PNA (green) and intact acrosomal membrane of sperm was not stained. Both of sperm swim-up method with or without BSA separated to Live intact mitochondria (15.39±4.31 vs, 12.58±3.74, and) without significant difference. and percoll density gradient method also similar (7.29±6.54), swim-up method of sperm preparation appeared to be more efficacious in percentage recovery of motile sperm concentration compared to Density gradient method.

Mitochondria are well known to regulate the mammalian embryo development. Recent studies showed that the mitochondrial dynamics responses are mainly generated through mitochondrial membrane potential (MMP) and cellular ATP production, which is dependent on mitochondrial reactive oxygen species (ROS). However, these mechanisms are unclear on development process of preimplantation porcine embryos. The aim of this study was to evaluate that difference of the mitochondrial dynamics-derived various functions on the embryo development according to lipid composition of zygote. First, zygote was classify two groups (high lipid, grade 1: G1 and low lipid, grade 2: G2) by lipid composition of cytoplasm. And, we performed the in vitro culture (IVC) using zygote of divided groups. The nuclei numbers and developmental rates of blastocysts were lower in G2 than those of G1 embryos. Next, we investigated the intracellular ROS and mitochondrial derived superoxide production in porcine embryos by using DCF-DA and Mito-SOX staining. As expected, both intracellular ROS and mitochondrial derived superoxide were significantly increased (p<0.05) in the preimplantation stage embryos of G2 group compared with G1 group. In addition, to observe difference of the mitochondrial functions, we investigated the mitochondrial membrane potential (MMP, ΔΨ) and contents of ATP in the preimplantation stage embryos by using JC-1 kit and ATP determination kit. These functions of mitochondria were dramatically reduced in cleavage stage embryos or blastocysts of G2 group. Finally, to verify the difference of the mitochondrial dynamics-derived various functions, we investigated the expressions of mitochondrial fission (Drp1, pDrp1-616) and fusion (Mfn1, Mfn2) factors by Western blotting analysis. Interestingly, the protein levels of pDrp1-616 in embryos of G1 group were continuously increased until blastocyst stage. Whereas, the expression patterns of Mfn1/2 in embryos of G2 group were significantly reduced during IVC progression. The expression patterns of mitochondria dynamic between the two groups were shown opposite. These results demonstrated that the lipid contents of zygote were related the positive-correlation with mitochondrial dynamics-derived functions in porcine embryos. Moreover, we suggest that lipid of zygote is play a important role on mitochondrial functions and dynamics during preimplantation embryos development in pigs.

PURPOSES: The density in uninterrupted traffic flow facilities plays an important role in representing the current status of traffic flow. For example, the density is used for the primary measures of effectiveness in the capacity analysis for freeway facilities. Therefore, the estimation of density has been a long and tough task for traffic engineers for a long time. This study was initiated to evaluate the performance of density values that were estimated using VDS data and two traditional methods, including a method using traffic flow theory and another method using occupancy by comparing the density values estimated using vehicular trajectory data generated from a radar detector. METHODS: In this study, a radar detector which can generate very accurate vehicular trajectory within the range of 250 m on the Joongbu expressway near to Dongseoul tollgate, where two VDS were already installed. The first task was to estimate densities using different data and methods. Thus, the density values were estimated using two traditional methods and the VDS data on the Joongbu expressway. The density values were compared with those estimated using the vehicular trajectory data in order to evaluate the quality of density estimation. Then, the relationship between the space mean speed and density were drawn using two sets of densities and speeds based on the VDS data and one set of those using the radar detector data. CONCLUSIONS: As a result, the three sets of density showed minor differences when the density values were under 20 vehicles per km per lane. However, as the density values become greater than 20 vehicles per km per lane, the three methods showed a significant difference among on another. The density using the vehicular trajectory data showed the lowest values in general. Based on the in-depth study, it was found out that the space mean speed plays a critical role in the calculation of density. The speed estimated from the VDS data was higher than that from the radar detector. In order to validate the difference in the speed data, the traffic flow models using the relationships between the space mean speed and the density were carefully examined in this study. Conclusively, the traffic flow models generated using the radar data seems to be more realistic.

A novel nanocomposite LDPE film with UV protective properties was developed for active packaging applications. Initially, undoped and Mn-doped TiO2 nanoparticles (NPs) were synthesized by the sol-gel method and the resulting particles were characterized. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images revealed an agglomerated nature and spherical morphology. X-ray diffraction (XRD) studies indicated that all products were crystalline and in the form of rutile. The reflectance spectrum of undoped TiO2 NPs demonstrated a characteristic sharp edge at 410 nm. Subsequently, nanocomposite (NC) LDPE samples were prepared with the NPs by solvent precipitation followed by film casting. The optical and thermal properties of the NC samples were investigated. Incremental increases in Mn concentration from 0.25 mol % to 1.00 mol % were associated with progressive decreases in light transmission in the UV region. The melting and maximum decomposition temperatures of all NCs were 107 and 442-449 °C, respectively. The UV protective LDPE-based NC films exhibited superior photostability. Absorption in the FTIR spectra at 1716 and 1734 cm-1 changed after 4-wk exposure to UV for all film samples as a consequence of photodegradation. Finally, the photooxidation of perilla oil was assessed as an example of a UV protective packaging application. After 12 days, protection with 1.00 mol% Mn-doped TiO2-LDPE was associated with a gradual increase in PV, while protection with TiO2-LDPE was associated with a significant increase and protection with the control treatment was associated with a dramatic increase in PV. Hence, a 1.00 mol% Mn-doped TiO2-LDPE NC showed promise for UV shielding packaging applications.

본 연구는 서해안에서 상선의 어구 손괴로 인한 추진기 장애를 예방하기 위해 연안자망어선의 월별분포를 제시하였다. 분석 방법은 2014년 1년간의 어선위치발신장치 데이터를 이용하였다. 조업 해구도를 기준으로 경·위도 30′× 30′크기의 해구별 간격으로 격자를 설정하였다. 총 56개 해구를 대상으로 격자를 구성하였다. 연구 결과 밀도지수가 가장 높았던 194해구는 어구 손괴가 없었다. 반면, 밀도지수가 상대적으로 작은 193해구는 상선의 주 통항경로에 어구가 설치되기 때문에 손괴가 발생했다. 이 분석을 바탕으로 어구 손괴는 밀도지수에 비례하지 않고 상선의 주 통항경로에 따라 손괴위험이 존재하는 것으로 나타났다. 따라서 상선의 안전항해를 위해 5월부터 9월까지 위도 34.5° ~ 35.5°, 경도 125.67° ~ 126°를 항행경보구역으로 통보하면 추진기 장애와 어구손괴와 같은 해양사고를 예방할 것이다. 따라서 상선이 193해구와 203해구를 항해할 때는 안전한 항해를 위해 위도 34.5° ~ 35.5°, 경도 125.5° ~ 125.67°을 추천한다.

The survey was conducted to investigate biomass and distribution of fisheries resources using a quantitative echo sounder and a fixed gillnet around Marado coast of Jeju to obtain the scientific basic data for dispute resolution with a large purse seine fishery and coastal fishing and policy establishment of reasonable fisheries resources. Hydroacoustic surveys were conducted six times (November 28~29, 2015 (night), February 23~24, 2016 (night) and March 3~4, 2016 (night/day), March 30~31, 2016 (night/day)) using a quantitative echo sounder. The pelagic fish densities were relatively higher around Marado in November 2015, February 2016 and March 3~4, 2016. However, demersal fish densities were relatively higher in Jeju coastal waters on March 30~31, 2016. Catch data using fixed gill net were used to calculate biomass. Based on the hydroacoustic data, fish length-weight function and target strength information of dominant fish, the biomass of fishes were estimated as follow: 5.64 ton CV = 70.2% at night on November 28-29 2015, 7.14 ton CV = 35.8% of pelagic fish and 530.77 ton CV = 34.6% of demersal fishes at night on February 23-24 2016, 2.34 ton CV = 56.7% of pelagic fish and 571.93 ton CV = 40.3% of demersal fish at daytime, 1.39 ton CV = 48.4% of pelagic fish and 194.59 ton CV = 54.3% of demersal fish at night on March 3~4 2016, 0.37 ton CV = 72.9% of pelagic fish and 338.79 ton CV = 99.7% of demersal fish at daytime, 0.24 ton CV = 21.3% of pelagic fish and 68.61 ton CV = 53.8% of demersal fish at night on March 30~31 2016.

This study was conducted for recycling the waste MDF(Medium-density fibreboard) to investigate the enzymatic saccharification characteristics using two enzymes Novozyme Cellic® CTec2 and HTec2 (Novozymes, Bagsvaerd, Denmark) after the delignification by pretreatment using sodium chlorite. The chemical composition of the waste MDF are lignin, holocellulose, ash, and other extracts 28.40, 60.20, 0.10, and 11.30%, after pretreatment with sodium chlorite were 5.20, 53.10, 0.03, and 41.67%. The Lignin interferes with enzymatic saccharification of 23.2% was removed, 7.1% of holocellulose was lost. The times of sodium chlorite pretreatment and saccharification of the waste MDF was finished between 48-72 hours, the saccharification speed was fast when the concentration of the enzyme by 10% and the HTec2 CTec2 ratio 9:1. Sugar ratio of the solid content of the waste MDF is the highest as 69.6% when it comes out of 8% and a viscosity was as high as 34.8% when the 12-FB%. Therefore, the pre-treatment with sodium chlorite is more advantageous when enzymatic saccharification to lignocellulosic biomass. The amount of the enzyme, the solid-liquid ratio, and the reaction time showed a proportional relationship with saccharification efficiency. The studies for increasing the solids content of waste MDF to improve the economic efficiency more than 12% should accompanied.

식물의 생육과 생산성을 예측하는 것은 매우 중요한 일이다. 본 연구는 common ice plant (Mesembryanthemum crystallinum L.)의 작물생장률, 상대생장률, 지상부 생체 중과 건물중, 수확시기, 상품률과 상품수량과 같은 생육과 수확 요인들을 쉽게 읽을 수 있는 재식밀도-생육-수확 (PGH) 도표를 만들기 위함이다. 광도 140μmol·m-2·s-1과 일 장 12시간 주기로, 3파장 형광등을 이용한 완전제어형 식물공장 시스템에서 박막수경 재배하였다. 4가지 재식밀도 (15×10cm, 15×15cm, 15×20cm와 15×25cm) 하에서 생육과 수량을 분석하였다. 재식밀도가 증가할수록 어느 한계까지는 식물체당 생체중과 건물중은 증가하는 경향이었으며, 단위면적당 생체중인 수량 또한 같은 경향을 보였다. 작물 생장률, 상대생장률과 lost time은 2차 등식 형태를 보였으며, 지상부 생체중과 건물중은 직선적인 관계를 보였다. 이러한 등식을 이용하여 재식밀도-생육-수확(PGH) 도표를 만들었다. 예를 들면, 15×20cm 재식밀도와 식물체당 생체 중 100g에서 수확할 경우, 재식주수, 작물생장률, 상대생장률, lost time, 식물체당 건물중, 수확시기와 수량은 각각 33plants/m2, 20g·m-2·d-1, 0.27g·g-1·d-1, 22days, 2.5g/plant, 정 식 후 26일과 3.2kg·m-2이었다. 이 도표를 가지고 적어도 2 가지 요인 예를 들면, 재식밀도와 수확요인 중 하나만 알면, 작물생장률, 상대생장률, lost time과 같은 생육 요인과 지상부 생체중, 지상부 건물중, 수확시기와 수량과 같은 수확 요인들을 쉽게 구할 수 있다. 이러한 도표는 다양한 작물의 생육과 수량 요인을 예측할 수 있어 완전제어형 식물 생산 시스템 설계를 위해 유용한 도구가 될 것이다.

This study identified effects of forest density and placed on the fluid flow in the canopy using a computational fluid dynamics model, ENVI_met model. In addition, change of the fluid flow for each point was observed by selecting the impact prediction point. As a result, the density of the forest gave a direct effect on the fluid flow in height below the tree height and inside crown. In case of height below the tree height, the strongest physical changes appeared in CASE1 of dense forest. On the other hand, in case of crown height, distinct fluid flow changes appeared in CASE 2 and 3. After calculating fluid change of the impact prediction point according to altitude, the more dense height below the tree height appear significant changes in the fluid. On the other hand, in case of crown, from the moment outside the interference of the tree, the result showed that the wind path formed distinctly in CASE 2 and 3.