The lung and lymph node samples were collected from 786 pig farms associated with wasting and respiratory syndrome during 2005～2009. All samples were tested for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) and the differentiation of its genotype using reverse transcriptase-polymerase chain reaction (RT-PCR). 643 farms (81.8%) of the pig farms examined were positive for PRRSV, of which 57.2% accounted for PRRSV type 1 and 70.2% accounted for PRRSV type 2. Furthermore, 37.5% of the farms positive for PRRSV, showed the coexistence of two genotypes. The results indicate that the PRRSV infections of single genotype or two genotypes are very common in Korean pig farms.

This study was performed to identify the differentially methylated region (DMR) and to examine the mRNA expression of the imprinted H19 gene in day 35 of SCNT pig fetuses. The fetus and placenta at day 35 of gestation fetuses after natural mating (Control) or of cloned pig by somatic cell nuclear transfer (SCNT) were isolated from a uterus. To investigate the mRNA expression and methylation patterns of H19 gene, tissues from fetal liver and placenta including endometrial and extraembryonic tissues were collected. The mRNA expression was evaluated by real-time PCR and methylation pattern was analyzed by bisulfite sequencing method. Bisulfite analyses demonstrated that the differentially methylated region (DMR) was located between -1694 bp to -1338 bp upstream from translation start site of the H19 gene. H19 DMR (-1694 bp to -1338 bp) exhibits a normal mono allelic methylation pattern, and heavily methylated in sperm, but not in oocyte. In contrast to these finding, the analysis of the endometrium and/or extraembryonic tissues from SCNT embryos revealed a complex methylation pattern. The DNA methylation status of DMR Region In porcine H19 gene upstream was hypo methylated in SCNT tissues but hypermethylated in control tissues. Furthermore, the mRNA expression of H19 gene in liver, endometrium, and extraembryonic tissues was significantly higher in SCNT than those of control (p<0.05). These results suggest that the aberrant mRNA expression and the abnormal methylation pattern of imprinted H19 gene might be closely related to the inadequate fetal development of a cloned fetus, contributing to the low efficiency of genomic reprogramming.

This study was undertaken to find out the effect of cholesterol and serum albumin on sperm ability and lipid peroxidation levels period to the liquid storage of miniature pig sperm. Ejaculated semen from miniature pigs was collected by gloved-hand method into a pre-warmed () thermos bottle, and extended with Modena solution {with and without BSA, methyl-beta-cyclodextrin (-cholesterol) and cholesterol loaded cyclodextrin (+cholesterol)}. Each semen was assessed for viability (SYBR-14/PI staining) and acrosome intactness, intensity and capacitation status by chlorotetracycline (CTC) staining at 1, 3, 5, 7 and 10 days of storage. At for the effects of cholesterol and serum albumin on lipid peroxidation, semen were incubated with (), and lipid peroxidation level were measured by flow cytometry using the lipid peroxidation reporter probe . The result, lipid peroxidation level in sperm added with cholesterol were lower in compared to the added sperm with serum albumin. Also, added cholesterol to sperm had significant (p<0.05) higher viability when storage for 7 and 10 days and lower when 10 days of storage percentage of acrosome-reacted sperm (AR pattern) in acrosome state as say result compared to other treated groups. In conclusion, role of cholesterol during lipid storage in miniature pig spermatozoa was protected boar spermatozoa from lipid peroxidation prior to lipid storage. Addition serum albumin during lipid storage in sperm may be induce sperm membrane damage by lipid peroxidation. Therefore, addition of cholesterol to miniature pig sperm will be lead to extension of liquid storage periods.

본 연구는 돈분슬럿지에 함유되어 있는 구리와 아연을 제거하여 합리적인 돈분슬럿지 자원화비료 생산법을 연구하였고 돈분슬럿지 자원화비료를 시비 후 사료작물의 발아특성 및 초기생육을 조사하여 돈분슬럿지 자원화비료의 안정성과 그 비효효과를 조사한 바 그 결과는 다음과 같다. 1. 공시시료인 돈분슬럿지의 이화학적 특성은 질소 4.44%,인산6.29%로 질소와 인산의 함량이 일반적인 자원화비료보다높게 나타나고 있으며 구리와 아연의 함량은 각각 787.04mg/kg,1850.08 mg/kg로 부산물비료 퇴비의 공정규격인 300 mg/kg,900 mg/kg보다 더 높게 함유하고 있다. 2. 유기산 7종류를 제조하여 중금속을 제거한바 옥살산2수화물의 경우에는 구리와 아연 각각 48%, 56%의 제거율을 보인바 옥살산2수화물을 구리와 아연의 제거용액으로 선정하였다. 3. 옥살산2수화물 유기산용액을 이용하여 돈분슬럿지가 함유하고 있는 구리와 아연을 제거할 경우에는 돈분슬럿지의 pH가 2.0이하로 급격히 낮아지는 현상이 나타나고 있는바 농업적으로 이용 가능한 자원화비료를 생산하기 위하여 소석회를 첨가하여 pH를 조정하여 하며 돈분슬럿지의 무게에 비례하여 2.5%의 소석회를 첨가할 시에는 pH가 5.1로 조정되었다. 4. 옥살산2수화물로 퇴비화 과정중의 발아지수를 측정한 결과처리구1과 2는 28일 이후에는 발아지수가 83과 79로 안전한 수준이었으나 처리구3의 경우에는 72로 낮게 나타나고 있다. 5. 알파파, 크림슨크로버, 헤어리베치, 옥수수, 자운영, 오차드그라스의 사료작물 6종의 발아특성과 초기생육을 알파파와 사료용옥수수는 무처리구 및 모든 처리구에서 높은 발아율을 나타내고 있으며 처리구1과2는 크림슨크로버의 발아율이 100%로 무처리구 보다 높은 발아율을 나타내고 있으나 처리구3은 알파파와 사료용 옥수수는 100%로인 것을 제외하고 자운영과 오차드그라스가 50%로 가장 낮은 발아율을 나타내고 있다. 초기건물량에서는 무처리구가 통계적으로 가장 낮은 수준이었으며 대조구와 처리구들 간에 통계적인 유의차가 존재하지 않는 것으로 나타났다.

본 연구는 사일리지 사료를 급여하였을 때 돼지 품종과 사일리지 급여 유무에 따른 등심의 품질 특성에 미치 는 영향을 평가하기 위해 실시하였다. 돼지품종은 가고시마 와타나베 흑돼지 (B), 삼원교잡종[D (Landrace× Yorkshire)× Duroc], 미국산 버크셔종(A) 등에 대해 각각 시험구와 대조구 20두씩 총120두를 공시하였으며, 개시체중은 평균 69.3± 3 kg, 시험기간은 평균 66.7± 9.3일, 출하일령은 200〜210일이었다. 사일리지를 급여하지 않은 대조구 (C)에 비해 급여한 처리구 (T)가 조단백질 함량은 많았고, 육색은 밝았으며, 신선육 관 능평가 시 외관, 육색, 상강도 및 전체적 기호도에서 높은 점수를 보였고, stearic산과 oleic산은 낮고, linoleic산, arachidonic산, 필수지방산 및 필수지방산/불포화지방산 비율은 높게 나타났다. 사일리지 급여 시 단맛 관련 아미노산 함량은 높은 장점을 지닌 반면 육색의 적색도는 낮고, 가열감량과 육즙감량은 많아 다즙성이 낮았으며, 육의 탄력성이 낮고 지방의 밝기는 어둡고 황색도가 높은 단점을 보였다.

Prediction of semen's fertilizing ability used in artificial insemination (AI) is one of very important factors on pig reproductive performance. In vitro fertilization (IVF) has been used for indirect evaluation of sperm's fertilizing ability and it has been showed as highly correlated index. In swine industry, increasing interest in preservation of boar semen raises questions on the sperm motility from semen used in commercial AI centers. Mitochondria in sperm mid-piece generate the energy to support motility and could be an explanation of impaired fertility. Objective of this study was to suggest usable sperm motility to farms in measuring the effect of sperm motility and sperm abnormality on in vitro production of embryo in which sperm's fertilizing ability can be determined indirectly. Semen samples were provided from local AI center and used within 3 days after collection. Semen samples were divided by 4 different motile groups (>70%; 61~70%; 51~60%; <50%) using CASA (computer-assisted sperm analysis) on the days of IVF. Developmental rate to the blastocyst stage from over 61% motile sperm group showed significantly higher rate than below 60% motile sperm group ( vs , p<0.05). In experiment to determine the relationship between sperm motility and viability and abnormality, over 61% motile sperm groups showed significantly higher viability rate compared to below 60% motile sperm groups ( vs , p<0.05). On the other hand, morphological sperm abnormality showed significantly higher in over 70% motile sperm group ( vs , p<0.05). In experiment to find the correlation between sperm motility of 4 different motile groups and amount of mitochondria, lower motility group also showed lower level of mitochondria (p<0.05). The mitochondria parameter used in this study showed another possibility to differentiate the sperm motility. Taken together, because below 60% motile semen used in AI reduce the fertility, AI centers should provide the over 60% motile sperm to the farms at the time of AI.

Early cleavage is a reliable prognostic tool for successful embryo transfer in assisted reproduction because early cleaved embryo show better pregnancy rate after transfer. There for, preparation of good embryo recipient is important factor to optimize efficiency of pig cloning. The present study was performed to evaluate the effect of early cleavage on the in vivo development of cloned embryos and to analyze breed, parity and estrous synchrony to optimize recipient for pig cloning. In vitro matured porcine oocytes derived from local slaughterhouse and fibroblasts derived from miniature pig fetuses were used for somatic cell nuclear transfer (SCNT). Reconstructed embryos were transferred to recipient pigs on the same day of SCNT or after 1~2 days of in vitro culture for selecting early cleaved embryos. Breed, parity and date of standing estrous of recipients were recorded for analysis. After 25~35 days after embryo transfer pregnancy was diagnosed using ultrasonography, and pregnant recipients were monitored till delivery. Between purebred and crossbred, no significant difference was founded in both pregnancy and delivery rates. However, early cleaved embryos showed significantly higher pregnancy (46.2%) and delivery (12.8%) rates compared to non-selectively transferred group (24.8% and 4.5%, respectively). The results also showed that the recipients showing standing estrous on the same day of SCNT and less than 4 parities were most suitable for pig cloning.

The relationship of several candidate genes polymorphisms with breeding values of economic traits were investigated in Korean Native Pigs. Record (2001-2006) of 546 Korean native pigs were obtained from National Institute of Animal Science, Korea having data on average daily gain (ADG), age at 70 kg (D70 kg) and backfat thickness (BF). The data's obtained were analyzed by the DF-REML (Derivative-Free Restricted Maximum Likelihood) program of Boldman using a single-trait animal model to analyse the genetic parameters. The analysis of restriction fragment length polymorphism (RFLP) was conducted on 68 Korean native pigs (KNP) using single nucleotide polymorphism (SNP). Different genotype frequencies of 5 candidate genes such as MC4R, PRKAG3, FABP3, ESR and PRLR3 were observed in KNP. Significant relationship of AA and AB genotype between MC4R polymorphic site and breeding value for average daily gain (ADG, p<0.05) was observed. PRKAG3 polymorphic sites were also found to be significantly related to breeding values for ADG, AA, AB genotype (p<0.05) and also, for Backfat thickness (BF), days to 70 kg and BB genotype (p<0.05). In conclusion, selection method would be more effective if it encompasses significant genotype for performance traits and that would further aid in the selection of seed stock in KNP.

본 연구는 산의 남쪽 면에 동서 방향으로 길게 건축한 두 동의 개방돈사 간의 이격거리와 높이차가 환기에 어떤 영향을 미치는지를 시뮬레이션 기법을 이용하여 비교분석하고자 수행되었다. 개방돈사 간의 이격거리는 6과 12 m로, 개방돈사 간의 높이차는 0과 2 m로, 그리고 평균풍속은 여름 1.3과 겨울 1.1 m/s로 각각 설정하였다. 여름철 조건 하에서, 남쪽에 있는 첫 번째 개방돈사 내부의 풍속은 이격거리 및 높이차와는 상관없이 0.75 m/s로 각각 나타났으나 북쪽에 있는 두 번째 개방돈사의 풍속은 높이차가 없을 때 첫 번째 개방돈사의 풍속과 같은 0.75 m/s였고 높이차가 2 m일 때 0.0 m/s에 매우 가깝게 나타났다. 겨울철 조건 하에서, 남쪽에 있는 첫 번째 개방돈사 내부의 풍속은 높이차와는 상관없이 0.15 m/s로 각각 나타났으나 북쪽에 있는 두 번째 개방돈사의 풍속은 이격거리가 12 m일 때 첫 번째 개방돈사의 풍속과 같은 0.15 m/s였고 이격거리가 6 m일 때 0.0 m/s에 매우 가깝게 나타났다. 따라서 개방돈사 간의 이격거리를 12 m 정도로 하고 계단형태보다는 평지형태로 정지작업을 하여 개방돈사를 건축하는 것이 바람직한 것으로 판단되었다.

Aflatoxins produced by Aspergillus spp. are recognized as a major concern in animal and human health. In pigs, ingestion of aflatoxin-contaminated feeds causes immunosuppression, hepatotoxicosis and poor feed efficiency. In this study, we screened the contaminated level of aflatoxins in 449 pig feeds from 12 swine farms in Korea. For rapid and efficient screening of aflatoxins in pig feeds we evaluated the feasibility of three commercial ELISA kits for the screening of aflatoxins in pig feeds. Twenty-nine pig feed samples were examined for total aflatoxins using three ELISA kits, simultaneously. From three repetitions of each assay, the average intra-assay precisions and the average inter-assay precisions expressed as coefficient of variation (CV, %) for VeratoxⓇ Quantitative Aflatoxin test were 6.90 and 12.29, respectively. A statistical comparison of the results between HPLC and ELISAs showed that the correlation coefficient values for VeratoxⓇ was 0.96. The results demonstrated that we can apply the VeratoxⓇ Quantitative Aflatoxin test for the detection of aflatoxins in pig feed from the field. The screening of field samples with this ELISA kit showed that 11 out of 265 pig feeds for growers and weaners were contaminated with total aflatoxin levels exceeding 10 ppb, maximum tolerable limits for their compound feeds and the aflatoxins levels of remaining 184 pig feeds for other age groups of pigs were confirmed as below 20 ppb. The results from the screening indicated overall low levels of aflatoxins contamination in pig feeds.

Since there had been the first human outbreak of novel influenza A/H1N1 in Mexico, it has become pandemic throughout the world. In the Republic of Korea, the first human case was on May, 2009 and National Veterinary Research and Quarantine Service (NVRQS) has started monitoring novel influenza A/H1N1 on domestic swine farms. The first positive case was detected on 14, Dec, 2009 and until now, we have had total 17 positive cases. From the first case, we did epidemiological investigation on introduction and transmission of virus to pigs. We have concluded that virus had been introduced from humans to pigs, possibly from October to November, 2009 in our country. There were direct and indirect factors including the vehicles transporting veterinary medicine, feed, etc. related to transmission of virus from farm to farm. However, breeding pigs seem to be extrinsic to transmitting factors. Upon the epidemiological investigation, the off-limits of visitors to farms and the disinfection is thought to be critical for blocking the introduction and transmission of novel influenza A/H1N1. In addition, collecting and analyzing the genetic informations of influenza virus is important for predicting and handling the new pandemic influenza in the future.