Three venom peptides, OdVP1, OdVP2, and OdVP3 were isolated from the venom of the solitary wasp Orancistrocerus drewseni (Hymenoptera: Eumenidae). The venom peptide amino acid sequences were determined by Q-TOF/MS/MS. The OdVP1, 2, and 3 with amidated C-terminals showed similar peptide sequences to the mastoparan from Vespula lewisii or the protonectin from Protonectarina sylveirae, suggesting that they adopt an amphipathic α-helix secondary structure. The amidation of C-terminal Leu of the venom peptides have been known to be required for their biological activities. The full-length open reading frame (ORF) sequences of the OdVP1, 2, and 3 were analyzed by 5’- and 3’-rapid amplification of cDNA ends (RACE). The overall gene structure of OdVPs showed a high homology to that of mastoparan B from Vespa basalis by containing signal sequence, prosequence, mature peptide and C-terminal glycine, but the mature peptide sequences were distinct from each other. The toxicological property and antimicrobial activity of OdVPs were characterized using synthetic peptides. This study on the venom peptides from O. drewseni should promote further studies on bioactive ingredients in the venom of solitary hunting wasps.

An entomopathogenic bacterium, Xenorhabdus nematophila, induces an immunosuppression by inhibiting phospholipase A2 (PLA2), which results in a fatal septicemia. PLA2 is an enzyme responsible for eicosanoid biosynthesis and the pathogenic molecular target of this bacterium. A PLA2 gene of the red flour beetle, Tribolium castaneum, was expressed in Escherichia coli. The recombinant T. castaneum PLA2 (TcPLA2) showed enzyme activity, which was specifically inhibited by bromophenacyl bromide (specific inhibitor to secretory PLA2) and ditheothreitol (reducing agent of disulfide bond). It was sensitive to pH (optimum at pH 7.0), temperature (optimum at 30°C), substrate specificity and calcium dependency. X. nematophila released compound(s) inhibiting TcPLA2during its stationary growth phase. The active compound (s) was resistant to heat treatment and could be extracted separately into both organic and aqueous phases. This PLA2 inhibitory fraction showed significant effect on immunosuppression of T. castaneum. These results suggest there may be several PLA2 inhibitors synthesized by X. nematophila and released into culture broth. The recombinant TcPLA2 was also used to screen potent PLA2 inhibitor compounds, which were designed based on a common chemical structure (a pentenebenzene ring) of two peptide inhibitors, proline-tyrosine (PY) and acetylated phenylalanine-glycine-valine (AcFGV). Alterations were made on amino acid sequence or specific functional groups on the pentenebenzene ring. Among 7 different peptides, AY and FGV showed the most potent effects on TcPLA2activity and also resulted in significant reductions in hemocyte spreading behavior of Plutella xylostella. The potent candidate molecules would be applied to control various insect pests to be developed into novel insecticides.

이상의 논의를 종합해 보면, 현대문예이론의 기준으로 梁啓超의 소설이론을 평가하면, 梁啓超의 소설에 대한 인식은 수준이 높다고 할 수는 없다. 매우 분명한 논리적 허점이 있어, 수많은 이론상의 오해와 논리상의 허점에도 불구하고, 당시의 객관적 현실을 감안하면 그의 이론이 시대의 최선봉에 선 것임은 분명하다. 심지어는 문장 속의 어떤 오해와 과장에는 말살할 수 없는 역사적 소명의식이 들어 있다고 해도 좋을 것이다. 梁啓超가 제창한 소설이론은 중국근대문학의 발전에 특별한 공헌을 했다. 첫째, 소설과 사회의 관계에 대한 그의 이론은 그때까지 볼 수 없었던 이론의 최고 수준이었다. 둘째, 줄곧 그 지위가 낮았던 중국의 소설을 “文學之最上乘”의 지위로까지 끌어 올렸다. 셋째, 그는 형식면에서, 신문이나 잡지에 이론을 발표함으로서, 중국의 문예비평방법인 評點이나 序跋로부터 완전히 벗어나서 근대이론으로 발전할 수 있는 토대를 마련했다. 넷째, 그 결과 소설이론이 구체적 작품으로부터 분리되어 문학의 일반론이나 서사방법 등 이론성 문장을 자유롭게 전개할 수 있는 방법론면에서 근대소설이론으로 발전하는 획기적인 계기가 되었다. 그러나 “소설계혁명”의 실제효과를 보면, 梁啓超이론의 부정적 효과도 너무나 분명하여 간과할 수는 없다. 첫째, 소설과 사회의 관계만을 일방적으로 강조하여, 소설이 그 본래의 독립적 품격을 상실하고 정치선전의 도구로 전락하고 말았다. 둘째, 소설과 사회라는 외부관계만을 일방적으로 중시하여 서사방법·인물의 전형화이론·사건의 형상화이론 등과 같은 소설내부의 예술문제 등을 홀시하여 중국의 고전소설이 근대소설로 정상적인 발전하는 것을 방해하였다. 청말(만청)에 이렇게 많은 창작이 쏟아져 나와 소설의 전성기를 구가했음에도 불구하고 이 시기의 소설에 대한 평가는 그다지 높지 않다는 것이 문학연구자들의 공통된 평가이다. 이러한 양과 질의 불균형 현상은 여러 가지 복합적인 원인이 있겠지만 그 가운데 가장 큰 이유 중의 하나는 갑작스런 양의 증대현상이 질적 향상을 담보할 수는 없었으며, 이런 양적 증가의 원인이 비문학적 요소인 사회변혁운동의 일환으로 전개되었다는 데서 그 원인을 찾을 수 있을 것이다. 그리고 이 사회변혁운동의 중심에 梁啓超와 그의 소설이론이 자리 잡고 있었다. 양계초는 자신이 주장한 “소설계혁명”이론을 바탕으로 하여 그의 유일한 소설창작인《新中國未來記》를 창작하였으나, 겨우 5회를 쓰고 나서 스스로 중단하고 말았다. 그의 이러한 실패는 서언에서 제기했던 문제에 명확한 답을 제시해 주고 있다. “실천만이 이론을 검증하는 유일한 방법이다.”

Bacillus thuringiensis 1-3 (Bt 1-3), isolated from Korean soil sample, showed high insecticidal activity against Plutella xylostella. Recently, we improved plasmid capture system donor-s (PCS-S) by inserting attB sites including lacZ between transposable elements (designated as pTroy), to reduce background and construct E. coli-Bt shuttle vector. Through in vitro transposition with total plasmid DNA of Bt 1-3, at least 6 different size plasmids of Bt 1-3 were cloned. Among them, 47 clones which have approximately 10 kb plasmid in size were sequenced and 5 contigs were assembled. These contigs showed partial similarity with two known plasmids, pGI3 or pBMB175, separately. These cloned plasmids will acquire erythromycin resistance by BP recombination reaction with pDonrattPEm vector. After transformation into Bt cells, final erythromycin resistant Bt cell might contain novel E. coli-Bt shuttle vector. This scheme proposes that pTroy and pDonr-attPEm system can easily construct new shuttle vector by in vitro transposition, BP reaction, and erythromycin selection with any Bt plasmids.

To find some antibacterial peptides responsible for bacterial resistance, we performed differential hybridization with total cDNA probes which synthesized from normal and immunized larvae. Thirteen individual cDNA transcripts were expressed differentially in a total 1,862 random cDNA clones. One of upregulated genes is a novel member of the insect defensin-like peptide(Coprisin), a family of antibacterial peptide. Northern blot analysis showed that Coprisin was up-regulated at 4h and reached the highest point level at 16h after injection of E.coli. The deduced amino acid sequence of Coprisin was composed of 80 amino acids with predicted molecular weight of 8.6 kDa and PI of 8.72. Comparison of the deduced amino acid mature portion of Coprisin with defensin-like peptide of other insect indicated that it has 79.1% and 67.4% identity with Anomala cuprea and Allomyrina dichotoma, respectively. To find antibacterial active region of Coprisin, we synthesized four peptides corresponding to amino acid residues 1V-43N-NH2(CopN1), 5-16(CopN2), 19-30(CopN3) and 31-43(CopN4) of coprisin having amidated amino acid residues at their Cterminal. A 12-mer amidated at its C-terminus, ACALHCIALRKK-NH2 (Ala19-Lys30-NH2) was synthesized based on the deduced amino acid sequence, assumed to be an active site sequence. This peptides showed antibacterial activity against E.coli, Staphylococcus aureus, MRSA, Psedomonas syringae, and Pectobacterium carotovorium. Modified 9-mer peptide, LRCIALRKK-NH2, showed strong antibacterial activity than mellitin peptide used as a positive control against gram-negative and gram-positive bacteria. This peptide showed no haemolytic activity and quite stable at 100℃ for several hours of incubation and in a wide pH range(pH2-12). Therefore, this peptide may be a good candidate for the development of new drug with potent antibacterial activity without cytotoxicity.

The neurotrophin plays an important role in the development, differentiation and survival of the nervous system in vertebrates. It exerts its cellular effects through two different receptors, the Trk receptor tyrosine kinase neurotrophin receptor and the p75 neurotrophin receptor, a member of the tumor necrosis factor receptor superfamily. Trk and p75 neurotrophin receptors utilize specific target proteins to transmit signals into the cell. An ankyrin-rich membrane spanning protein (ARMS) was identified as a new p75 interacting protein and serves as a novel downstream target of p75 neurotrophin receptor. We sought to delineate the interaction between p75 and ARMS by deletion constructs of p75 and green fluorescent protein (GFP)-tagged ARMS. We examined the interaction between these two proteins after overexpressing them in HEK-293 cells. Using both Western blot analysis and immunocytochemistry followed by confocal laser scanning microscopy, we found out that the intracellular domain of the p75 neurotrophin receptor was important for the interaction with ARMS. The results from this study suggest that ARMS may play an important role for mediating the signals from p75 neurotrophin receptor into the cell.

To develop an improved baculovirus insecticide with additional advantages, a novel recombinant baculovirus, AcB5B-AaIT was constructed. B. thuringiensis crystal protein gene (cry1-5) and insect-specific neurotoxin gene (AaIT) were introduced into Autographa californica nucleopolyhedrovirus genome by fusion of polyhedrin-cry1-5-polyhedrin under the control of polyhedrin (polh) gene promoter, and AaIT under the control of early promoter of ORF3004 from Cotesia plutellae bracovirus, respectively. About 150 kDa of Polyhedrin-Cry1-5-Polyhedrin fusion protein expressed by AcB5B-AaIT was occluded into the polyhedra produced by the recombinant virus, and activated as about 65 kDa of crystal protein when treated with gut-juice of Bombyx mori. The AcB5B-AaIT showed about 50% reduced LT50 value compared to that of the recombinant virus, Ap1Ac, expressing Cry1Ac against Plutella xylostella larvae. In addition, Spodoptera exigua larvae fed the recombinant polyhedra of AcB5B-AaIT showed about 4 fold higher refusing diet effect compared S. exigua larvae fed the recombinant polyhedra of the recombinant virus, Ap1C, expressing Cry1C. AcB5B-AaIT could be transferred to wild-type baculovirus along with serial passage by the homologous recombination between two polyhedrin genes contained in polh-cry1-5-polh fusion protein gene. These results suggested that the novel recombinant baculovirus, AcB5B-AaIT, could be applied as advanced viral insecticide.

Among the cultured products of Beauveria bassiana SFB-205 (KCCM 10892P), the supernatant showed the highest insecticidal activity against 2nd instars of Aphis gossypii (Aphididae) nymphs under glasshouse condition. The enzymes in the supernatant were confirmed as active materials, and the chitinase was finally selected as a QC factor for commercial production. However, the chitinase activity in the supernatant decreased by 11-folds due to the thermal stress at 50℃ for 2 h. To obtain thermal stability, the chitinase in the supernatant was adsorbed to a precipitable material and the pellet was freeze-dried (PCT/KR2007/005886). The adsorbent-A showed about 92.7% of harvesting efficiency which was higher than the other candidates. The chitinase activity of the freeze-dried powder was kept up about 82.0% of initial activity for the same thermal stress. Subsequently, an optimal formulation recipe was established to maximize long-term storage stability and efficacy. SFB-205 oil-based formulation was stable up to 18 months at room temperature. It showed 96.1% efficacy against 2nd instars of A. gossypii nymphs at 1 day after the treatment in the glasshouse. This novel approach can be a promising method to develop competitive biopesticies in the entomopathogenic fungi, even though it needs to be intensively studied.

분리막 공정은 에너지 절약형 분리공정으로 전통적인 증류 분리공정과 비교하여 높은 선택도를 나타내기 때문에 액상 혼합물 분리의 대체 공정으로서 주목받고 있다. 제올라이트 막을 비롯한 무기분리막은 유기분리막의 단점을 보완하고 혹독한 조업조건에서도 운전이 가능한 장점을 지니고 있다. 최근 기존의 제올라이트 분리막의 단점을 보완하고 더욱 향상된 분리성능을 위한 새로운 무기분리막 재료들이 연구되어지고 있다. Kalsilite는 Si/Al 비율이 1로써 기존의 4A 제올라이트와 같이 친수성을 나타낼 것으로 예상되며 세공의 크기가 4A보다 더 작은 0.36 nm로 분리막으로 제조 시 가스분리, 물/유기물 혼합물에서 물의 선택적 분리가 가능할 것이다. 본 연구에서는 Si : Al : K : H2O = 1 : 1 : 8 : 60의 원료 포성 비율을 사용하여 kalsilite 분말을 경제적으로 수열합성 할 수 있는 새로운 공정을 개발하였으며. 최적의 합성조건인 합성온도 300℃, 합성시간 6시간으로 kalsilite를 합성할 수 있었다. XRD 분석을 통하여 kalsilite임을 확인하였으며 입도 분석 결과 평균입도는 2.73 μm이었다. 증기흡착 결과 kalsilite는 유기물보다 물에 대한 흡착능력이 큰 친수성 알루미노실리케이트임을 알 수 있었다.

4 세대 무선망에서 이 기종 엑세스 망간의 핵심기술은 망 선택기법과 호 수락제어이다. 본 연구에서는 차세대 무선망의 이 기종 엑세스 무선망으로 CDMA-OFDMA 망을 고려하여, 이 망에 적합한 퍼지 신경망 분산 상향링크 호 수락제어를 제안한다. 제안된 기법은 이종 무선망에서 원하는 신규호의 차단확률과 서비스 진행 중인 호의 강제종료확률을 만족시키면 서, 무선자원의 효율적인 사용을 목적으로 한다. 호 수락의 결정은 무선 엑세스 망의 상향 링크에서 퍼지 추론과 신경망 학습에 의한 추정된 전력값 과 미리 결정한 상향 전력 한계값 을 비교하여 모바일 터미널에서 결정한다. 시뮬레이션을 통하여 제안한 기법의 성능을 신경 망 입력노드의 수에 따른 평균 거절율과 섹터 당 평균 이동국 수에 따른 호 손실율 및 거 절율을 평가 하였다. 그 결과 제안된 기법을 사용하는 무선망은 원하는 서비스 품질을 만족 하면서 부하의 증가에 따른 효율적인 호 수락제어가 이루어짐을 보인다.

Indirubin is the ac ti ve ingredi ent of a traditional Chinese herbal medicine, Danggui Longhui Wan, used for t he t reatment of chronic myelocytic leukemia Here, we report that novel indirubin ,- 11‘ ivative‘ 5’ - nitro-indirubinoxime (5’ NIO) , has potent anti- proliferative activity on va rious human cancel‘ cells and oncogenic RK3E- ras rat kidney cells with ha lf- inhibi tory concentrati ons (1C50) ra nging from 1- 12 M, Treatment with indirubin derivative induced the activation 01' caspase 7 rollowed by apoptos is in RK3E- ras cells. lndirubin derivative showed strong anti-tumor activity in rat solid and oral tUll10r models , Direct inj ection of indirubin deri vative every other day for 10 days induced signifi cant inhi bition of tumor growth in Sprague-Dawley rats bearing RK3E- ras-induced tumors Histologically. t reatment with indiru bin de ri vative caused s ignifi cant inhi bit ion of tumor formation with increased apoptosis and decreased tumor cell prolife ration, These f indings provide the potent ial va lue of indirubin deri vative a s a novel candidate for ant i-tumor agents