최근 국제원자재가 상승에 따라 버섯 배지 가격이 50% 이상 상승하여 톱밥 대체 배지의 개발이 절실한 실정으로 은행껍질을 이용하여 버섯 배지로 개발하기 위해 수행한 결과를 요약하면 다음과 같다. 은행껍질의 질소 함량은 0.32%로 포플러 톱밥 0.09%에 현저히 높았으며, 시험관을 이용한 균사생장은 은행껍질 10~30% 첨가시 93~97㎜/28일로 대조구 101㎜/28일에 비해 늦었다.은행껍질 10% 첨가시 850㎖ PP병의 균배양일수는 18일로 대조구와 같았지만 20~30% 첨가시 2일 지연되었고, 초발이 소요일수는 은행껍질 10~30% 첨가시 7일로 대조구보다 1일정도 빨랐다. 병당 수량은 대조구 70.8g/병에 비해 은행잎박 10~30% 첨가시 80.5~91.8g/병으로 14~30% 증수되었고, 배양 및 생육일수는 은행껍질 10% 첨가시 30일이 소요되었다. 따라서 미소느타리 병재배시 은행껍질 10%를 첨가하는 것이 재배일수 단축 및 수량증수 효과가 있는 것으로 사료된다

Small & Medium construction firms have been faced with demand of environmental changes. construction firms have been required to improve their structures to reinforce the competitiveness. so, these firms are making desperate efforts, which are reorganization, renovation, improvement. this study would be emphasized the Quality Management in construction work. For this, It must be supposed a plan to improve a construction organization. The Construction Firm would be executed Quality Management learning, leadership, understanding of Quality Management to construction workers.

The purposes of this study is to verify whether insider trader get the excess return using inside information. For this we divide inside traders into four groups according to their ownership: maximum shareholder, main shareholders, 5% sharehoders and executives. Also we categorize inside traders into three groups: personal investor, foreign investor and institutional investors. After insiders trade their stock, excess return is reported for 20days and the size of excess return of executives and institutional investor is larger than that of other groups. It means more strict monitoring system is needed in the domestic stock market.

Since 1995, the small and medium enterprise introduced the Quality Innovation Program that is called 100PPM Quality Renovation Program. A point of 2000, the program's name was changed by Single PPM Quality Innovation Program in pursuit of defects below 10ppm in outgoing quality. Nowaday 1,651 companies is received Single PPM Quality Certification from government at the end of 2008. On the other side, the return or withdrawal of certification is going on because of various reasons. In this study, we analyse the status of certification's preservation and cancellation, and suggest the cooperation ideas between government and companies.

In this era of knowledge and information of the 21st century, the source of a nation's competitive power consists of human resources and material resources and the importance of human resources appreciates highly. The number of small and medium enterprises which are the core of national economy covers over 98% among whole domestic enterprises of Korea. Competitive power of small and medium enterprises comes from human resources development. For the development of human resources, small and medium enterprises are trying to expand their learning organizations in the company. The analysis of outcomes of administering the existing learning organizations shows that learning organizations contribute highly to the improvement of the productivity of small and medium enterprises. And the analysis also shows that the participants of learning organizations and middle management and executives who support and encourage them recognize the necessity of learning organizations and that it should be disseminated throughout other enterprises. Therefore government's supports to establish learning organizations in a company are required to expand.

This study was conducted to examine the effects of human follicular fluid and gonadotropin (FSH+HCG+rhEGF) on in vitro maturation, fertilization and development of human immature oocytes. Cumulus-oocyte complexes (COCs) were collected following for in vitro fertilization and embryo transfer (IVF-ET) cycles of the patients. At the time of oocytes collection, oocytes were classified into MII, MI and GV in accordance with their appearance (MII: Fully mature oocyte at metaphase II of meiosis; MI: Nearly mature oocytes at metaphase I of meiosis; GV: Immature oocytes at prophase I of meiosis). After controlled ovarian stimulation using gonadotropin(FSH) and human chorionic gonadotropin (HCG) in 70 ICSI cycles, 158 MI to MII matured oocytes were intracytoplasmic sperm injection (ICSI) h after in vitro culture and 553 MII oocytes were ICSI after denudation. The aspirated MI and GV oocytes were cultured in culture medium containing 10% (v/v) serum protein substitute (SPS), 10% (v/v) human follicular fluid (hFF) and 10% (v/v) serum protein substitute (SPS)+1 IU/ml FSH+10 IU/ml HCG+10 ng/ml recombinant human epidermal growth factor (rhEGF). The maturation rate of immature oocytes was similar among the three group. When maturation medium was supplemented with 10% SPS, 10% hFF or gonadotropins, the fertilization rate of in vitro matured oocytes was higher in 10% SPS (80.0%), but there was no statistical significance (78.2%; hFF, 76.9%; gonadotropin, p>0.05). The development rate of human embryos developed to cells were not significant difference in the medium containing SPS, hFF and gonadotropins (65.6%, 65.9% and 66.7%). The results of these study suggest that human follicular fluid and gonadotropins supplemented in the culture medium was not effected on the in vitro maturation, fertilization and development of human immature oocytes.

The present management of the domestic and international advanced enterprises is transferring the systems and the regulations to the prevention system management. Expecially, KOSHA (Korea Occupational Safety & Health Agency) in Korea has supported to set up the OSHMS (Occupational Safety & Health Management System) by KOSHA 18001 since 1997. But small & medium enterprises (SMS) has many difficulties to set up the OSHMS system because of limited natural and human resources. In this study, the rating system as a judgment method of an OSHMS based on the certification standard of the OSHMS, which was suggested in previous study, was developed for SMS to adopt voluntarily the OSHMS, certify KOSHA 18001, and improve the safety and health level.

In resent year there has been research on benefits of conjugated linoleic acid (CLA) and docosahexaenoic acid (DHA) for human health. The effect of a fat supplement made from seaweeds meal plus palm olein meal (FS) and replacement of concentrate on milk production, and milk fatty acid (FA) profiles of dairy cows fed grass silage based diets was evaluated. Ten Holstein lactating cows were divided into a two groups. The cows in a group were fed concentrate at 40% on TDN basis (control). The cows in another group (Treatment) were supplemented 0.5㎏/day/cow of FS as a substitute for 2㎏/day/cow of concentrate. The medium chain fatty acids and DHA content of FS were 345 ㎎/g DM and 35 ㎎/g DM, respectively. Total DMI, DM digestibility and milk production as well as milk composition did not differ between the two groups. The intake of ether extract was higher (P＜0.01) in Treatment than control group (1.1 ㎏/day/cow vs. 1.3 ㎏/day/cow). The short and medium chain of FA in the milk did not differ between two groups. The trans11C18:1vaccenic acid, cis9trans11CLA and C18:3 concentrations in the milk were higher (P＜0.01) in Treatment than control group(1.88 g/100gFA vs. 1.03 g/100gFA, 0.93 g/100gFA vs. 0.51 g/100gFA and 0.48 g/100gFA vs. 0.48 g/100gFA). However, the C18:2 concentration in the milk was lower (P＜0.05) in Treatment than Control group (1.38 g/100gFA vs.1.17 g/100gFA). There were detected 0.13 g/100gFA of DHA in the milk in Treatment group. Results indicated that addition of the FS has the potential of increasing CLA and DHA in cow's milk.

The objective of this study was to examine the effect of macromolecule in a maturation medium on nuclear maturation, intracellular glutathione (GSH) level of oocytes, and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Immature pig oocytes were cultured in maturation medium that was supplemented with each polyvinyl alcohol (PVA), pig follicular fluid (pFF) or newborn calf serum (NBCS) during the first 22 h and the second 22 h. Oocyte maturation was not influenced by the source of macromolecules during in vitro maturation (IVM). Embryo cleavage and cell number in blastocyst after PA was altered by the source of macromolecule but no difference was observed in blastocyst formation among treatments. Oocytes matured in PVA-PVA medium showed lower rates of oocyte-cell fusion (70.4% vs. 7782%) and embryo cleavage (75% vs. 8690%) after SCNT than those matured in other media but blastocyst formation was not altered (1327%) by different macromolecules. pFF added to IVM medium significantly increased the intracellular GSH level of oocytes compared to PVA and NBCS, particularly when pFF was supplemented during the first 22 h of IVM. Our results demonstrate that source of macromolecule in IVM medium influences developmental competence of oocytes after PA and SCNT, and that pFF supplementation during the early period (first 22 h) of IVM increases intracellular GSH level of oocytes.

This study was aimed at analyzing the structural relationship among such factors as industrial environment, technological cooperation, technological innovation performance and management performance that are essential to technological innovation of small and medium-sized companies. For this aim, an analysis was conducted to determine which of the factors in industrial environment has impact on technological cooperation of small and medium-sized companies. An empirical analysis was also performance to find what kind of effects the technological cooperation may have on technological innovation and management performance. From the analyses, it became known that: first, changes in industrial environment have influence on technical cooperation factors including production technology, technical information, technical manpower and fund for technology that are assorted by means of factorial analysis; second, the technological cooperation of small and medium-sized companies has impact on their technological innovation performance; and third, the technological cooperation of small and medium-sized companies has impact on management performance.

분주 크기와 배지 종류가 고추냉이 생존율과 생육에 미치는 영향을 알아보고자 환경이 조절된 생장상에서 60일간 재배하였다. 수태로 감싼 근경 크기(직경) 5mm 이상 분주 묘의 생존율은 100%였으며, 생육(초장 12cm 내외, 엽수 3~4장)은 양호하였다. 생육 60일의 분주 묘는 초장 20cm 이상, 엽수가 1~2장 증가하였다. 루톤 분의제 처리는 생존율과 생육에 효과가 없었다. 배지 종류(마사, 난석)에 따른 직경 5~10mm 크기의 분주 묘를 담액 수경으로 재배하였을 때 83% 이상의 생존율을 보였으며 배지 종류에 따른 생육 차이는 없었다. 따라서 근경 크기 5mm 이상의 고추냉이 분주를 환경이 조절된 생장상에서 30일간 수경재배 방식으로 순화하여 고추냉이 건전 묘를 생산할 수 있다.

Pteris cretica ‘Wilsonii’의 대량생산에 적합한 배양 환경을 구명하기 위하여 연구를 수행하였다. 포자는 7주 안에 모두 발아하였다. Knop과 Hyponex배지에서 전 엽체 증식이 왕성하였지만, 전엽체의 생육에는 Hyponex 배지가 더 유용하였다. MS배지에서는 전엽체가 괴사하 였으며, 질소급원과 sucrose의 농도를 조절한 경우에도 sucrose 무첨가구를 제외한 모든 첨가구에서 전엽체가 괴사하였다. Sucrose 1%와 agar 0.6%를 첨가한 Hyponex배지가 전엽체의 증식과 생육에 가장 적합한 것으로 나타났다. 접종방법을 달리한 결과, Hyponex배 지에서는 다져서 접종하는 전엽체의 증식 및 생육에 효과적이었지만, MS배지에서는 전엽체의 군집을 4등 분하여 접종한 처리구에서 전엽체의 증식 및 생육이 우수하였다. 고체배지에서 배양하는 것이 액체배지에서 배양하는 것보다 효과적이었다. 액체배양은 전엽체의 괴사를 유도하였다. 액체진탕배양한 전엽체는 생육은 우수하였으나 고체배양한 전엽체에 비하여 증식이 억 제되었다.

본 연구는 청나래고사리의 기내 포자체 유묘를 이용한 효율적인 대량생산 방법을 개발하기 위하여 시행되었다. 유묘의 엽신, 엽병, 근경을 곱게 다져 활성탄 0.1%를 첨가한 1/2MS배지에 배양한 결과, 근경의 절편에서만 포자체 유묘가 생산되었다. 곱게 다진 근경 절편은 1/2MS 배지에서 포자체 재생이 가장 왕성하였으며, sucrose 농도를 2%로 조절하고 NaH2PO4 50 mgL−1을 첨가할 경우 포자체 재생이 더욱 촉진되었다. Kinetin과 BA를 단용 또는 NAA, IBA와 각각 혼용하여 배양한 결과, kinetin 1 μM 단용 처리구에서 포자체 재생이 가장 왕성하였다. BA 첨가구에서는 분열조직의 증식이 왕성 하였으나. 분열조직이 포자체로 분화되지 못하는 특징을 보였다. 변형 1/2MS 배지(sucrose 2%, NaH2PO4 50mgL−1, kinetin 1 μM, pH 5.8, agar 0.8%)에 활성탄 0 또는 0.1%를 첨가하여 고체, 액체 정체, 액체 진탕배양한 결과, 포자체 재생은 액체 진탕배양시 가장 왕성하였으며, 활 성탄의 첨가는 포자체 재생을 촉진하였다.

This study is about the development of information system software process quality evaluation model for domestic medium enterprises based on CMMI being used widely in the world. In this study, a new evaluation model is developed through establishing tailoring guide and its application to the existing mode by reflecting the quality management method and conditions of domestic medium enterprises within CMMI Level 2 and Level 3. The evaluation model is constituted 3 processes, 11 achievement goals, 50 practice activities and 125 evaluation items. The average point of quality evaluation of enterprises of insurance business shows 79.1 based on the evaluation model. The average point of the enterprises certified from CMMI is 82.7 which is 3.3 point higher than non-certified enterprises. This result means that the new evaluation model has similar results with the existing evaluation model while it also has discriminational characteristics compared with the existing model.

Sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a serious pest of many economically important crops. The insect has developed resistance to chemical insecticides. Therefore, the development of microbial agent is necessary. Among the several entomopathogenic fungi, Lecanicillium lecanii Btab01 which has high insecticidal activity was carried out this experiments. To develop mass culture, we subcultured L. lecanii Btab01 on PDA, TSA, SDA+Y, RA and GSA media at 25℃ incubator to select the optimal solid culture medium. Hyphal growth was measured every 3 or 4 days. L. lecanii Btab01 grew fastest in RA, followed GSA, SDA+Y, PDA and TSA. L. lecanii Btab01 was cultured on PDB, TSB, SDB+Y, RB, GSB media at 25℃, 180rpm shaking incubator to select the optimal liquid medium. Spore germination was measured by spread plate method every 12 or 24 hours. Spore germination appeared 7.8×108 CFU/ml after 4 days in RB, followed GSB (5.5×108 CFU/ml), SDB+Y (2.7×108 CFU/ml), TSB (1.7×108 CFU/ml) and PDB (0.6×108 CFU/ml).

오늘날 중소기업의 자동화 및 정보화 설비구축을 보면 과거보다는 높은 발전을 이루고 있다. 그러나 이러한 독립된 설비, 장비, 시스템의 기능을 살려 관리와 경영의 효율을 높이기 위해서는 인력과 비용이 많이 소요되어 운영계획을 포기하거나, 규모를 축소하여 생산성향상에 저해되는 상황이 발생하기도 한다. 따라서 본 연구에서는 최근에 반도체 기술의 발전으로 임베디드 마이크로프로세서의 개발로 보다 용이하고, 빠르게 시스템을 설계하거나 변경을 할 수 있도록 하드웨어와 소프트웨어의 인터페이스를 구현하였다. 특히, 중소형 제조기업에서 실시간으로 생산정보를 통해 경영에서 필요로 하는 정보를 생성하는 프로세스를 제시하였다.