가축에서 생산성을 높이기 위해 사용되는 수정란이식술의 실용성을 알아보기 위하여 한우 및 젖소 36두로부터 과배란 처리를 하여 신선수정란은 25두에, 동결수정란은 17두에, 쌍태 생산을 위한 수정란 두 개 이식은 13두 수란우에 이식되어 그 생산 결과를 조사하였다. 1 과배란을 위한 호르몬 처리후 반응을 나타낸 공란우는 75.0％이었다. 2. 황체수와 비교하여 채란된 수정란 율은 91.8％ 이었다. 3. 비외과적으로 채란된 전체 난자 중 생존성이 있는 배

본 연구는 재래산양에 있어서 과 배란 처리에 의한 oocyte의 회수방법과 양질의 oocyte 회수 체계를 확립하기 위하여 과 배란 처리 후 회수시간이 난포의 발달과 난자의 회수율에 미치는 영향을 조사하였다. 공시동물은 체중 15~25 kg 전후의 성숙한 미경산 재래산양으로서 발정동기화를 위하여 CIDR를 10일간 질내에 삽입하고 과배란 처리는 FSH를 CIDR 삽입 8, 9, 10일째에 12시간 간격으로 70 mg을 감량 투여하였다. 는 FSH와 함께

본 연구는 재래산양에 있어서 과배란 처리시 공란 산양의 반복사용에 따른 oocyte의 회수방법과 회수란의 질적 개선방법을 확립하기 위하여 실시하였으며, 성선자극호르몬의 1회 처리와 반복처리, 호르몬제의 종류에 따른 oocyte의 회수율과 등급을 조사하였다. 공시동물은 체중 15~25kg 전후의 성숙한 미경산 재래산양으로서 발정 동기화를 위하여 CIDR를 10일간 질내에 삽입하고 과배란 처리는 70 mg의 FSH 와 1,000 IU의 PMSG를 투여하여

본 연구는 고능력 한우 공란우의 이용효율을 극대화하기 위하여 반복 과배란처리가 수정란회수율에 미치는 영향을 알아보고자 수행하였다 공란우의 선발은 DNA marker에 의한 고급육을 선발하였다. 선발된 공란우는 Folltropin-V와 PG를 병용 처리하여 과배란을 유도하였고, FSH 처리 6회째 PG를 처리하여 발정을 유도하였다. 발정유도된 공란우의 수정은 12시간 간격으로 3회, 1회당 2 straw식 인공수정을 실시하였다. 수정 후 7~8일째 비외과

In this stuyd, the effect of the dominant follicle aspiration for the superovulatory response in HanWoo was investigated. The criterion for the presence or absence of a dominant follicle based on their morphological examination. The dominant follicle was aspirated 48hr before the onset of superovulation treatment by 6.5MHz convex probe connected with a carrier and superovulation induced by FSH (Super-Ov Tyrer, Texas, U.S.A) adminstered twic a day s.c. over 4 day in a decreasing regimen. From 13 HanWoo scanned daily to determine the presence and growth of the dominant follicle, its an average diameter of 15.4mm was measured and an average diameter of corpora lutea was 18.7mm on day of follicular aspiration. In the experiment, a follicular remove by ultrasound-guided aspiration, the ovarian response was significantly enhanced when animals were superovulated in the aspiation of a dominant follicle compare with animals superovulated non-aspiration of a dominat follicle. In the aspiration of a dominant follicle donors yieleded more corpora lutea(14.44.7 vs 8.63.4) and transferable embryos(8.94.2 vs 5.42.7) than control. In cows in which the dominant follicle had been aspirated under sonographical control 2 days before superovuation, the number of corpus lutea and transferable embryos were significantly enhanced compared with animals superovulated in the presence of a dominant follicle (14.44.7 vs 6.92.7, ; 8.94.2 vs 3.31.6). After 7 days of artificial insemination, the embryos at 7 days were cllected by uterine flushing after dominant follicle insemination, the embryos at 7 days were collected by uterine flushing after dominant follicle aspiration and superovulation treatment, and evaluated their quality by morphological criteria. Sixteen embryos with excellent and good grade were transferred into 8 recipient cows. Six pregnancies were identified at 60 and 120 days of gestation by rectal palpations. In conclusion, the present study showed that 1) the presence or absence of a dominant follicle signficicnatly affects superovulatory responses, and 2) ultrasound-guided follicular aspiration of the dominant follicle and superovuation treatment provides an accurate and procedure to increase ovarian responses in HanWoo.

The present study was carried out to evaluate the effect of superovulation treatments on ovarian responses, oocyte recovery rates and grades of collected oocytes using an ultrasound-guided transvaginal approach in Korean native cows. Superovulation in cows was induced with two different regimenes: 1) FSH-decreasing dose(n=8): the cows were received twice per day for three days of the total dose of 400 mg of FSH-p, 2) FSH-single dose(n=9): the cows were administrated a single dose of 400 mg of FSH-p in 25% PVP. The Observation of visible follicles and collection of oocytes were performed 12 hours following the last FSH in FSH-decreasing dose group and 48 hours after the FSH-single dose injection. All visible follicles larger than 6 mm were punctured and aspirated with a 6.5 MHz convex-array ultrasound transducer designed for intravaginal use. The mean number of visible follicles(> 6 mm) was significantly(P<0.05) higher in the FSH-decreasing dose treatment (22.811.9) and FSH-single dose treatment (20.612.0) groups than the non-treatment group(7.08). The mean recovery rate of oocytes was not significantly(P<0.05) different between the treatment and control groups, but the mean number of collected oocytes was significantly(P<0.05) higher in the FSH-decreasing dose treatment( 12.611.5) and FSH-single dose treatment (11.813.6) groups than the non-treatment group(3.70.5). In conclusion, the FSH-single dose treatment at superovulation in cows for ultrasound-guided aspiration might increase the number of aspiratable follicles and the recovery rate of follicular oocytes as the FSH-decreasing dose treatment.

This experiment was arried out to investigate the development of ea4y rabbit embryos in vivo. Twenty-six New Zealand White does were superovulated by treatment with PMSG(Intervet Co; I. M single injection, 150. U./rabbit) followed 3 day later by simultaneous I.V injection of 100 I.U HCG (Intervet Co, )and natural service with fertile male. All of does was killed at the specific times (24, 27, 30, 36, 42, 50 and 93 h post-hCG) to find out the early embryonic development in vivo respectively. Embryos at the specified stages of development were obtained at the following times after injection of hCG; one-ceH at 24 h, two-cell at 24~27h, four-cell at 27~36 h, morulae at 50 h and early blasto-cyst at 93 h and expanded or hatching blastocyst at 144 h. Number of embryos recovered per rabbit superovulated was 26.1 and average of recovery rate was 83.7%. The results suggest that superovulation was efficient for the increase of embryo number in rabbits, and as shown in results, asynchronous cleavage was prevalent among the recovered embryos.

This study was conducted to investigate the effect of unilateral ovariectomy on the embryo survival and changes of plasma progesterone and estradiol concentration. Mature Angora rabbits were divided into 2 groups (Superovulation and unilateral ovariectomy after superovulation). Unilateral ovariectomied rabilts were subdivided into two groups according to the time of ovariectomy (24 hours and 96 hours after mating). The results obtained were summarized as follows; 1. After unilateral ovariectomy, survival rate and collecting rate of embryos recovered from contralateral and ipsilateral oviduct and uterus were not different, and were lower than intact rabbits. 2. Plasma progesterone concentration at 93, 99, 102 and 114 hours after HCG injection in superovulated rabbits were 12.9 0.5, 34.8 5.1, 12.2 2.7 and 43.4 5.8ng/ml, respectively. Mean progesterone concentrations were significantly higher at 99 and 114 hours than at 93 and 102 hours (p <0.05). But plasma estradiol concentration was not different. 3. Plasma progesterone concentration in unilateral ovariectomized rabbits was somewhat decreased after unilateral ovariectomy. Plasma estradiol concentration was not different.