The vast majority of embryo generated by Assisted Reproductive Technologies (ART) do not result in a live offspring and a multiple birth is the single biggest health risk associated with human fertility treatment, and the used of frozen embryos increased for medical or personal reasons. However, practical and ethical reasons might hamper study of human embryos. Therefore, animal models are necessary to elucidate the molecular and morphological changes during development. In the serial experiments, we employed mouse embryos and a Cdx-inducible ES cell system that transdifferentiates into TS cells. We found aberrant gene expression profiles including apoptosis associated (Bcl2), lineage formation related genes (Cdx-2, Tcfap2c, Oct4, and Nanog), and/or mitochondrial DNA replication related genes (mt-cox-1, mt-cox-2, Polg, Polg2, Tfam) in mouse embryos that showed developmentally retardation between morula to blastocyst transition or post implantation development after embryo transfer to surrogate mothers, compared to control embryos. To determine direct interaction between knockdown genes via siRNA approach and putative down-stream genes involved in blastocyst formation and further development, we carried out qPCR and Chip assay in either mouse embryos or the ES cells. qPCR and Chip assay results showed target gene directly bound to promoter regions of down-regulated genes in TS cells. In conclusion, we suggested that an increased understanding of epigenetic regulation of early embryonic development through animal models may ultimately lead to better methodologies for selecting more competent embryos and and/or protocols for augmenting embryos viability.

본 연구의 목적은 높이맵 기반의 액체 시뮬레이션을 이용하여 인간 친화적으로 표현하기 위한 감성 컨텐츠 제작 에 있다. 액체를 인간 친화적으로 표현하는 기존 연구들은 액체 표면을 표현하는데 몇 가지 문제점이 있다. 이러한 문 제를 해결하기 위해 본 연구는 액체의 광학적 특징을 이용한 새로운 액체의 카툰 렌더링 기법을 제안한다. 본 연구 에서 제안하는 액체의 카툰 렌더링은 반사와 굴절을 독립적으로 표현한 채색 단계와 액체의 경계선과 파형선을 표현 하는 선화 단계를 거쳐 표현된다. 그 결과 인간 친화적으로 표현된 액체의 표면을 실시간으로 감성 컨텐츠로 제작할 수 있다.

건설업은 공사수행을 포함한 대부분의 기업 활동이 인력에 대한 의존도가 높은 산업으로서 원가구성 측면에서도 외주비와 재료비를 제외하고 가장 큰 원가항목이 인건비다. 따라서, 건설업의 인력관리 강화는 효율적인 인력 운용과 원가관리를 위해서도 가장 중요한 관리항목이다. 본 연구에서는 건설업에서 체계적인 인력 및 시간관리를 통해 인력운용의 효율을 높일 수 있고, 업무시간 실적 정보에 의해 업무에 대한 성과관리의 정확성을 높일 수 있는 제도운영 및 시간관리 시스템 (TMS: Time Management System) 구축 방안을 소개하였다. 이러한 시간관리는 높은 수준의 인력 및 업무 관리를 가능하게 하고 업무에 대한 우선순위 결정과 적정 시간 분석으로 관습적이고 비효율적인 업무요소를 파악하여 효율적인 인적자원 관리를 하고자하는 건설사뿐만 아니라 프로젝트 산업 군에 속하는 여러 기업들에게 도움을 줄 것으로 기대한다.

This study was conducted to examine the effects of human follicular fluid and gonadotropin (FSH+HCG+rhEGF) on in vitro maturation, fertilization and development of human immature oocytes. Cumulus-oocyte complexes (COCs) were collected following for in vitro fertilization and embryo transfer (IVF-ET) cycles of the patients. At the time of oocytes collection, oocytes were classified into MII, MI and GV in accordance with their appearance (MII: Fully mature oocyte at metaphase II of meiosis; MI: Nearly mature oocytes at metaphase I of meiosis; GV: Immature oocytes at prophase I of meiosis). After controlled ovarian stimulation using gonadotropin(FSH) and human chorionic gonadotropin (HCG) in 70 ICSI cycles, 158 MI to MII matured oocytes were intracytoplasmic sperm injection (ICSI) h after in vitro culture and 553 MII oocytes were ICSI after denudation. The aspirated MI and GV oocytes were cultured in culture medium containing 10% (v/v) serum protein substitute (SPS), 10% (v/v) human follicular fluid (hFF) and 10% (v/v) serum protein substitute (SPS)+1 IU/ml FSH+10 IU/ml HCG+10 ng/ml recombinant human epidermal growth factor (rhEGF). The maturation rate of immature oocytes was similar among the three group. When maturation medium was supplemented with 10% SPS, 10% hFF or gonadotropins, the fertilization rate of in vitro matured oocytes was higher in 10% SPS (80.0%), but there was no statistical significance (78.2%; hFF, 76.9%; gonadotropin, p>0.05). The development rate of human embryos developed to cells were not significant difference in the medium containing SPS, hFF and gonadotropins (65.6%, 65.9% and 66.7%). The results of these study suggest that human follicular fluid and gonadotropins supplemented in the culture medium was not effected on the in vitro maturation, fertilization and development of human immature oocytes.

Generally, manpower education/training courses and contents required for game industry is designed to operational tasks. This reflects that the structure of manpower in game industry becomes specialized. Because of the rapid changes in the game industries and in internal and external environment, most game industries require workers that can perform a variety of job types to create a business values. In this research, we consider major reports related to the education and training needed for workers in game industry and then conduct overall survey on satisfaction with the education/training courses and contents of study. The results from analysis on the current education/training courses and contents for manpower in game industry will help development of policies for designing systematic education/training courses and contents, and are expected to be used for future education and training for manpower for game industry.

For development of a human body model for electric shock, electroconductive paints with carbon black as a filler material were developed. The characteristics of the volume resistivities of thin films fabricated using the electroconductive paints were investigated as a function of the particle sizes and content of carbon black. With a carbon black particle size over 80 μm, agglomeration of carbon black powders was observed. The volume resistivity of the particles increased as the porosity increased and as the amount of carbon black decreased due to the agglomeration of carbon black powders. With a particle size of 4 μm and 20 μm, agglomeration of carbon black powders was not observed and their porosities were measured as 0.86% and 1.12% with volume resistivities of 20 Ω·cm and 80 Ω·cm respectively. A carbon black particle size of less than 20 μm is considered to be suitable as a type of electric-shock electroconductive paint for a human body model.

본 연구에서는 생활용품이면서 의복과 함께 생활필수품인 신발을 대상으로 감성만족도 요소의 추출과 분류체계를 개발하였다. 제화류의 감성만족도 요소는 감각성(sense), 적합성(fitness), 묘사성(description), 평가성(evaluation), 선호성(attitude)의 5개 그룹으로 구성되었으며, 각 그룹에는 형태감, 볼륨감 등 29개 요소가 포함되었다. 이들 중에서 일반 제화의 평가에 적합한 요소들로 형태감, 볼륨감, 균형감, 색감, 인체적합성, 안락성, 안정성, 여유성, 개념적 이미지, 품위감, 고급감, 조화감, 견고성, 주목성, 간편성, 신뢰성, 선호도, 수용도, 만족성, 매력성 등 20개의 요소들을 선택하여 이들에 대한 평가양식을 개발하였다. 본 연구에서 제시한 감성만족도 요소는 제화류의 고객만족도 평가 모델링에서 종속변수로 활용될 수 있다.

Accident analyses are used to identify common factors contributing to occupational accidents and to give recommendations for accident prevention. In this study we developed a human error analysis system that can be used easily at the industries. This accident analysis system can be used to develop accident prevention programs to reduce human initiated accidents.