본 실험은 정식 후 단일처리전 장일처리 기간에 따른 온실재배 스프레이 절화국 (cv. Reagan Improved)의 생육 및 건물생산에 미치는 영향을 구명하기 위하여 네덜란드 Wageningen 대학의 유리온실 실험포에서 수행하였다. 정식에서 개화기까지 3주 장일처리구에서는 84일이었고, 2주 처리구에서는 77일, 1주 처리구에서는 70일,단일처리 후에 최종수확일까지는 63일이 소요되었다. 정식 후 초장 생장량은 3주 장일처리구에서 가장 높았고, 1주 장일처리구에서 가장 낮았다. 또한 정식 후 최종 엽수, 엽면적 확보량 및 엽생체중과 총식물생체중은 3주 장일처리구에서 높고, 1주 장일처리구에서 낮은 결과를 나타내었다. 반면.단일처리 후 절대생장율에 있어서 엽전개속도와 초장생장속도는 모든 처리구에서 동일한 반응을 보였다. 최종건물생산량 (g.m-2 )은 3주 장일처리구에서 높고, 1주 장일처리구에서 가장 낮게 나타났다. 초장과 생체중은 장일 조건이 길수록 높게 나타났으며,누적 건물중도 3주 장일처리구에서 가장 높은 결과를 나타냈다.

To investigate the effect of Mycorrhiza (Glomus intradics) and Rhizobium inoculation on the N, P utilization and growth response of Alfalfa (Medicago saliva L.) and Perennial ryegrass (Lolium perenne L.) in mixed sward, four treatments (non-inoculation, C

To investigate the effect of Mycorrhiza (Glomus intradics) and Rhizobium inoculation on the N, P utilization and the growth of Alfalfa (Medicago sativa L.), four treatments (non-inoculation, control ; Mycorrhiza inoculation, M ; Rhizobiurn inoculation, R

영양생장기(營養生長期)의 형태적(形態的)과 건물수량(乾物收量)과의 관계(關係)를 조사(調査)하여 수량(收量)에 관여(關與)되는 형태적(形態的) 형질(形質)과 품종(品種)의 특성(特性)을 비교(比較), 검토(檢討)하였다. 1. 개체중(건물중(乾物重))은 초장(草長), 엽면적(葉面積) 및 경면적(莖面積)과 유의(有意)한 정상관(正相關)을 나타내어, 개체중의 증가(增加)는 초장(草長), 엽면적(葉面積), 경면적(莖面積)의 상호작용(相互作用)이라고 할 수 있다

Brassica rapa is one of the most valuable vegetable crops worldwide. Cultivated varieties of B. rapa exhibit diverse developmental and morphological appearances, which includes important vegetables, oilseeds, and fodder crops. In this study, various phenotypes of recombinant inbred lines (RILs) of B. rapa were investigated, including their responses to five different pathogenic Botrytis cinerea isolates, responses to aphid and thrips during flowering stages, days to flowering, and plant heights. Responses of 113 RILs to five different B. cinerea isolates showed variations, suggesting that genetic factors controlling resistance or tolerance against each isolate were dependent on isolate/genotype pairs. Correlation analysis was performed to understand the nature of genetic factors and the relationship among these phenotypes. Although high levels of correlation were not detected between phenotypes assessed in this study, statistically significant correlation was detected for several combinations. Significant positive correlations were found for different B. cinerea isolates, supporting that certain levels of commonality could exist in genetic components controlling resistance against different B. cinerea isolates. Based on correlation analysis using numbers of insects counted on plants, it was speculated that genetic factors responsible for aphid tolerance or repellence might be also involved in the response against thrips. Relationship between vegetative growth and tolerance against B. cinereal or insects is rather more complicated. However, it was observed that shorter plants appeared to have a certain level of tolerance or repellence against both aphids and thrips. Data presented in this study could be used to assist further genetic studies and breeding efforts to obtain Botritis and insect resistance for B. rapa.

‘Hwasu 3551’(HS) 과 ‘White-Red Lip’(WR)을 대상으로 저 온이 호접란의 생육단계별 광합성에 미치는 영향을 알아보았다. 실 험에 사용하기 위해 조직배양을 통해 번식된 유묘들을 4주간 외부 환경에 순화시킨 후(1개월 묘), 28/26oC(HT)의 식물생육상에서 재배하였다. HT에서 0(1개월 묘), 2(3개월 묘), 4 개월(5개월 묘) 간 재배된 식물들을 21/19oC(LT)의 식물생육상으로 옮겨 3개월 간 저온처리 하였고, 각 식물생육상의 일장은 12시간(06시.18시), 광도는 110±10μmol·m-2·s-1 PPF를 유지하였다. 저온의 영향을 알 아보기 위해서 각 온도 및 생육단계별로 영양생장과 24시간 동안의 CO2흡수율을 측정하였다. 전반적으로 호접란의 광합성은 CO2흡 수 양상에 따라 페이즈 I부터 IV까지 나뉘어지는 전형적인 CAM 광합성을 보였으며, 3개월간 저온에서 재배 후 하루 동안 흡수한 총 CO2의 양과 영양생장의 증가정도는 HT 조건을 유지한 처리군에 비해 감소하였다. 특히 3개월 묘를 LT에서 3개월간 재배하였을 떄, HS, WR 두 품종의 페이즈 III 동안 CO2흡수율은 각각 -1.36, -0.60μmol·m-2·s-1까지 감소하여 CO2누출 (CO2 leakage)양상을 보였다. 이로 인해 낮 동안의 총 CO2흡수량이 감소하였고, 영양 생 장량 역시 감소하였다. 1개월 묘를 LT조건에서 재배하였을 때 신 엽의 발생은 통계적으로 유의성 있게 감소하였지만, 5개월 묘의 경 우에는 감소하지 않았고, 단지 엽장의 증가정도만 감소하였다. 이 러한 결과는 5개월 묘에서 저온의 영향이 작았고, 영양생장이 계속 유지되었다는 것을 나타낸다. 호접란의 재배에 있어서 이러한 접근 방식은 재배 스케줄을 조절하거나 기존의 방법을 개선하여 재배비 용을 절감하는 대에 도움이 될 수 있을 것이다.

Gastrodia elata has been cultivated as an important medicinal resources to treat various human diseases. One of the major problems associated with its field production is the degeneration of seed tubers, which is mainly caused by soil-borne pathogens. This study was conducted to produce disease-free seed tubers by the development of in vitro micropropagation method. First, tubers of G. elata were treated with HgCl2 prior to culturing in vitro. Among various culture medium tested, water agar (WA) and WPM medium were the most effective on the induction and growth of vegetative stems. NAA (0.1mg/l) or TDZ (1.0mg/l) in WA medium showed better growth of vegetative stems compared to other plant hormones. Finally the induction and growth of vegetative stems were better in the dark compared to the light condition. In this study, we established an in vitro micropropagation system of G. elata, which might be an efficient way to increase the yield and quality of G. elata tubers in the field production.

An experiment was carried out at the Field Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh from March to July, 2001 to investigate the effect of biofertilizers on morpho-physiological characters of okra. The experiment was laid out in a randomized complete block design with four replications. There were nine treatments such as ~textrmT0 (control), ~textrmT1 (Azotobacter biofertilizer), ~textrmT2 (Azospirillum biofertilizer), ~textrmT3 (Azotobacter+Azospirillum biofertilizers), ~textrmT4 (Azotobacter+Cowdung 5 ton ~textrmha-1 ), ~textrmT5 (Azospirillum+Cowdung 5 ton ~textrmha-1 ), ~textrmT6 (Azotobacter+Azospirillum+Cowdung 5 ton ~textrmha-1 ), ~textrmT7 (Cowdung 5 ton ~textrmha-1 ) and ~textrmT8 (60% Nitrogen). The experimental results revealed that significant variations exist among the treatments regarding morphological characters e.g. plant height, number of leaves/plant, stem base diameter, tap root length, and physiological characters like, root dry weight, leaf area index and crop growth rate. Number of leaves/plant, stem base diameter, root length, root dry weight, leaf area index and crop growth rate were found higher in ~textrmT4 , ~textrmT5 , ~textrmT6 and ~textrmT8 than the others. In all the parameters, ~textrmT8 gave the similar result with biofertilizers in combination with cowdung treatments and ~textrmT7 showed identical with ~textrmT0 (control). Biofertilizer treatments had insignificant effect on 1000-seed weight(g). Experimental results mentioned above revealed that morpho-physioligical characters of okra could be modified by the application of biofertilizer+cowdung. However, biofertilizers+Cowdung treatments were comparable to ~textrmT8 (60% Nitrogen) in this study. This suggests that ~textrmT4 or ~textrmT6 or ~textrmT5 were more benificial in environmentally friendly okra cultivation and may be used as an alternative of inorganic nitrogen by saving cost of production and sustaining productivity.

Water deficit is the primary constraint of soybean [Glycine max (L.) Merr.] yield, and a physiological understanding of processes affected by water deficit is a key step in identifying and improving drought tolerance in soybean. The objectives of this research were to evaluate biomass and nitrogen accumulation patterns and water use efficiency (WUE) as possible mechanisms associated with the drought tolerance of Jackson. Biomass accumulation of Jackson was contrasted with the PI416937, which also has demonstrated tolerance to drought. For water-deficit treatment, total biomass accumulation was negligible for PI416937, but biomass accumulation continued at approximately 64 % of the well-watered treatment of Jackson. Transpirational losses for Jackson and PI416937 were approximately the same for the water-deficit treatment, indicating that Jackson had superior WUE. Isotopic discrimination of 13 C relative to 12 C also indicated that Jackson had higher WUE. Results indicated that increased WUE for Jackson under water deficit showed it was tolerant to drought rather than had an avoidance mechanism.

Leaf area is critical for crop light interception, and thereby has a substantial influence on crop yield. This experiment was conducted to characterize the development of soybean [Glycine max (L.) Merr.] leaf area. Plastochron index and leaf relative growth rate of Jackson was contrasted with the PI416937, which also has demonstrated tolerance to drought. First, plastochron ratio (PR) and plastochron index (PI) were evaluated in greenhouse to compare the leaf growth rate between two genotypes under well-watered condition. There was reasonable constancy of PR between two genotypes. The PR means of Jackson and PI416937 were 0.41 and 0.44, respectively. A fairly smooth increase of PI during vegetative stage was observed. Second, the relative growth rates were graphed against leaf area, normalized with respect to final leaf area, under well-watered and water-deficit conditions. Leaf growth was sustained longer in well-watered condition than water-deficit condition and there was a sizable proportion of leaves which was ceased earlier their growth in water-deficit condition compared to well-watered condition. The leaf relative growth rate of Jackson until leaves had completed at 45% of their growth during water deficit period was higher than that of PI416937.

검정콩 1호를 공시품종으로 하여 콩의 영양생장기에 15일간 습해처리하고 그에 따른 엽생장 및 지하부의 생리적 반응을 조사한 결과는 다음과 같다. 1. 습해처리기간중 근류는 심토층에서부터 사멸되기 시작하였으며 습해기간이 7일 이상 지속될 경우 전체 근류의 35%이상이 사멸되었다. 2. 습해를 받으면 근류의 Bacteroid층이 균열되기 시작하였으며 습해기간이 지속될 경우 균열 양상은 더욱 심화되었다. 3. 습해기간중 토심 5cm이하에 분포한 뿌리활력은 무처리에 비해 크게 감소하였으며 표토층에 분포한 뿌리는 큰 변화가 없었다. 4. 7일간의 습해처리로 주근의 외피조직 및 유관속조직의 분리현상과 일부 파괴현상이 관찰되었다. 5. 습해처리 기간 중의 엽면적 생장은 정상식물체의 엽면적 생장의 절반에 불과하였다.

작물의 영양생장기에 처리된 산성비에 대한 내성과 피해양상을 알기 위하여 벼, 옥수수, 콩, 팥, 고추, 토마토, 참깨, 밀, 보리, 배추, 무, 상추, 시금치 등 13개 작물을 파종 후 20일에 인공산성비 (pH 3.0, 4.5, 6.0)를 10mm씩 2일 간격으로 15회 처리한 후 작물의 형태적 및 생리적 변화를 조사분석한 결과를 요약하면 다음과 같다. 1. pH가 3.0이상일 때 인공산성비 처리에 의한 가시적 피해는 없었다. 2. 전자현미경상 조직의 피해는 없었으나 참깨, 콩, 팥의 엽록체내에 염색정도가 옅은 지질과립이 나타났다. 3. 엽신의 엽록소 함량은 팥에서는 증가하였으나, 벼, 토마토, 시금치에서는 감소되었고, 다른 작물은 인공산성비 처리간에 차이가 없었다. 4. 광합성은 팥에서 증가되었고, 배추와 보리에서는 감소하였으나 다른 작물은 인공산성비의 영향이 없었다. 5. pH 2.0의 인공산성비를 처리하였을 때 가시적 피해가 적은 작물은 벼, 옥수수, 참깨, 토마토, 밀, 보리 등이었으며, 피해가 큰 작물은 팥, 고추, 콩, 배추, 상추, 무, 시금치 등이었다.