In this study, we investigated and analyzed the registration, sales and regulatory management system of in vitro diagnostic veterinary medical reagents (IVDVMRs) in Korea. The registration of IVDVMRs has gradually increased since 2000, and total of 233 products from 58 companies were registered from 1975 to 2014. The market size of IVDVMRs is estimated to be approximately 12 billion Won per year from 2011 to 2013: the export sales and proportion was estimated to be 36.8% as 4.4 billion Won in 2013. Of these products, the ranking of the sales were canine heartworm, bovine tuberculosis, swine fever, porcine reproductive and respiratory syndrome, canine distemper+adenovirus+parvovirus disease, foot and mouth disease, etc. In vitro diagnostic human medical reagents were diverted biological medicine from the medical devices by the revision of the Pharmaceutical Affairs Law Enforcement Regulations in 2014 in Korea. In contrast, in vitro diagnostic devices for animal were still managed as medical devices and biological medicines, respectively. The diagnostic reagents for infectious diseases have neither classification nor grade systems. Good manufacturing practices (GMP) requirements on IVDVMRs were also exempted from the current system. This study suggested that the registration of the IVDVMRs has increased since 2005, and regulations of these devices should be improved for the effective operating system.

Tomato ringspot virus (ToRSV)는 Group IV(+) sense ssRNA viruses, Nepovirus속으로 분류되며 식물병원성을 가진 국내 미보고 관리급 검역바이러스이다. 본 연구에서는 우리나라 검역현장에서 ToRSV를 검사하기 위한 reverse transctiption(RT)-polymerase chain reaction(PCR)과 nested PCR 진단시스템을 개발하였다. ToRSV 특이적으로 진단할 수 있는 RT-PCR은 프라이머 조합9 (F120/R20, 549 bp)와 조합31(F60/R80, 741 bp)]이며, 각각의 nested PCR 결과 439와 363 bp를 증폭할 수 있다. 한편, 본 연구에서 개발한 유전자변형-양성대조구는 실험실 오염으로 인한 거짓양성반응을 확인할 수 있다. 본 연구에서 개발한 ToRSV 검역진단시스템은 향후 식물검역에 기여할 것으로 기대된다.

새로운 형태의 포스파젠 진단막을 제조해 혈액 속의 글루코우즈의 농도를 측정하였다. 혈액 속에 존재할 가능성이 높은 장애성분들을 4가지 그룹으로 분류하여 혈당 측정에 미치는 영향을 조사하였다. 가장 낮은 농도를 나타내는 시리즈 I의 경우, 대부분의 첨가물들이 혈당 측정에 큰 영향을 미치지 않았으나, 오직 ascorbic acid (AA)만이 기준 용액보다 5~8% 정도 높은 K/S 결과치를 나타내었다. 시리즈 II의 경우, 네 가지 첨가물 모두 기준 용액의 K/S 결과치보다 3~4% 다소 낮은 값을 보였으나 농도에 따른 일정한 값을 보여 주었다. 시리즈 III의 경우, 세 가지 첨가물 모두 기준 용액에서 얻어지는 K/S 결과치와 큰 차이를 보이지 않았다. 가장 높은 농도를 나타내는 시리즈 IV의 경우, 두 가지 물질 모두가 기준 값보다 11~15% 정도 낮은 수치를 나타내었으나 혈당 측정에 크게 영향을 주지는 않았다.

Odor problems in urban areas have become a common cause of public complaints. In order to gain a better understanding of odor problems, we investigated the emission characteristics of odorants originating from manholes (n=22) and stormwater catch basins (n=48) (SCBs) around Wangsimni station in Seoul. To this end, concentrations of H2S and NH3 were measured after arbitrarily dividing the whole study area into five different districts. Our measurements were made to allow comparisons between three different criteria: (1) manholes vs. SCBs, (2) temporal variation between morning vs. afternoon, and (3) water content in the sewer system. The average concentration of H2S in manholes and SCB were 2.39 ppm and 0.81 ppm, respectively. In contrast, their NH3 counterparts were 1.86 ppm and 1.02 ppm, respectively. According to the contouring method made to identify spatial distribution characteristics, of odorants a relatively lowland site D was expected to work as a significant emission source in light of the transportation patterns of a sewer system. Moreover, higher concentrations of odorants were prevalent in the afternoon (as the prime time for commercial activities) relative to before noon. If concentration data are converted into odor intensity, H2S was the predominant component exerting controls on the odor pollution in these underground environments.

목적 : 작업치료사의 역량지표를 토대로 작업치료사의 역량수준을 진단해 보고, 다양한 변인 간의 차이를 분석하고자 한다. 연구방법 : 작업치료사의 역량 진단을 위해 Ku (2014)가 역량모형 연구 과정에서 개발한 작업치료사 역 량지표를 사용하였다. 역량지표로 질문지를 구성하여 임상 작업치료사에게 직접 실시하거나 우편으로 실 시하여 총 337부를 회수하였다. 응답 결과를 통해 역량지표의 중요도와 보유도의 인식수준 차이를 알아 보고, 성별, 근무 병원 유형 등 다양한 변인 간의 집단 차이를 검증하였다. 결과 : 작업치료사가 평정한 역량지표의 중요도는 4점대 중반의 높은 수준을 보인 반면, 자가진단한 역량 보유도 인식수준은 3점대 후반으로 중요도보다는 전반적으로 낮은 평균 수준을 나타냈다. 변인 간 차이 를 분석한 결과, 남자가 여자보다 전문가의식과 자기관리 역량이 높았으며, 3년 이상 경력자가 이론적 지식, 작업치료 수행, 자기관리에서 높게 나타났다. 연령은 30대 이후에서 대체적으로 역량 보유도 인식 수준이 높게 나타났다. 결론 : 본 연구는 작업치료사로서 갖추어야 할 역량지표의 중요도와 자신이 보유한 역량을 스스로 진단해 봄으로써 작업치료사가 역량을 유지하고 발전시켜 나가는데 중요한 기초자료가 될 것이다. 또한, 작업치 료사들을 교육 훈련시키기 위해 필요한 교육내용의 방향성을 제시해 줄 것이다. 주제어 : 역량지표, 역량 진단, 작업치료사

Pelargonium zonate spot virus (PZSV)는 group IV (+) ssRNA viruses, Bromoviridae에 속하는 식물 병원체로, 일반적으로 토마토, 국화, 아티초크 및 제라늄에 감염된다. 본 연구는 검역 현장에서 PZSV를 신속하고 특이적으로 진단 할 수 있는 PCR module을 개발하는 것을 목적으로 하였다. PZSV를 검출하기 위한 RT-PCR 프라이머 선발 결과, 각각 513 및 320 bp를 증폭하는2개 조합을 선발하으며, 더욱 높은 검출감도로 검출할 수 있을 뿐아니라 RT-PCR을 검증할 수 있는 nested PCR 프라이머 조합을 개발하였다. 또한, 제한효소 Xho I 부위를 삽입한 유전자변형-양성대조구 플라스미드를 설계하여, PCR module에서 대조구로부터 오염을 검증할 수 있도록 개발하였다. 본 연구에서 개발한 PCR module은 토마토, 국화, 아티초크 및 제라늄 등에서 PZSV를 간편, 신속 및 특이적으로 검출하여, 지속적으로 식물검역에 활용할 수 있을 것으로 기대된다.

The bumblebee, Bombus terrestris, plays an important role as one of alternative pollinators since the outbreak of honeybee colony collapse disorder. Recently, pathogens and parasites such as viruses, bacteria and mites affecting the life span and fecundity of their host have been discovered in B. terrestris. In this study, in order to detect viral infection in B. terrestris, we collected B. terrestris adults and isolated total RNA for diagnostic PCR. The PCR primers specific for pathogenic viruses were newly designed and applied to gene amplification for cloning and detection. Capsid protein gene of black queen cell virus (BQCV) among examined viral genes was only successfully amplified from collected bumble bee adults and sequenced. To optimize the detection of capsid protein gene of BQCV, 4 regions in the capsid protein gene were selected and further analyzed in quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis revealed that capsid protein gene was directly detected with not more than 200 ng total RNA. This result suggests that an optimized detection via qRT-PCR can be applied for the rapid and sensitive diagnosis of BQCV infection in the field population as well as risk assessment of B. terrestris.

물에 녹지 않는 폴리(페녹시-메틸아미노)포스파젠을 합성하여 혈당 측정을 위한 새로운 형태의 진단막으로서의 가능성을 조사하였다. 혈액속의 글루코우즈의 농도를 변화시켜가며 활성화된 폴리포스파젠 진단막을 가지고 680 nm에서의 최종흡광도를 측정하였다. 시간에 따라 변화하는 흡광도의 값(K/S)을 이용해 글루코우즈의 농도를 얻었다. 친수성인 메틸아미노기와 소수성인 페녹시기의 치환율이 글루코우즈의 농도 측정에 미치는 영향을 조사하였다. 친수성기의 치환율이 증가함에 따라 글루코우즈의 농도와 K/S와의 기울기 값(Dose-Response Slope : DRS)이 점차적으로 증가하였다. 그러나 친수성기의치환율이 5% 이하일 때는 DRS가 너무 낮았고 친수성기의 치환율이 25% 이상일 경우에는 DRS가 급격히 증가하여 정확한글루코우즈의 농도측정이 어려웠다.

For ex-vivo diabetic control, the voltammetric diagnosis of glucose (GU) was conducted with a modified carbon nanotube paste electrode, using handheld analytical circuits. The optimum analytical conditions were attained within the 0.5-4.0 ug/L working range and at the 0.06 ug/L detection limit, which system was interfaced to the feedback circuits and was applied to human urine for diabetic-patient diagnosis. It can be used for ex-vivo flow control analysis, vascular flow detection, and other medicinal assays.

Autoimmune pancreatitis should be differentiated from pancreatobiliary cancers because they often have similar clinical features and images. Accurate and practical diagnostic algorithm for AIP is important to avoid unnecessary surgery and delayed treatment. International Consensus Diagnostic Criteria for AIP suggested that diagnostic algorithms and the practical patterns considerably vary worldwide. Patients with clinically suspected AIP can be categorized into patients with typical features of AIP and patients with indeterminate features based on CT findings. Serology and other organ involvement can be used as collateral evidence of AIP. If a patient presents with diffuse pancreatic enlargement but is lack of collateral evidence, pancreatogram could be useful. If a patient has obstructive jaundice, biliary drainage and endobiliary biopsy are recommended. Duodenal papillary biopsy for IgG4 immunostain can be used during ERCP. In case of atypical imaging findings, EUS-guided FNA/biopsy is recommended to exclude pancreatic cancer and to obtain the suggestive findings of AIP. If type 2 is clinically suspected, EUS-guided core biopsy is required for the definite diagnosis. Short-term steroid trial can be performed to confirm the diagnosis of AIP when pancreatobiliary cancer workup shows negative results. However, clinical practice in diagnosing AIP varies depending on the local expertise, facilities, cost, prevalence of AIP and its subtypes.

The diagnosis of autoimmune pancreatitis (AIP) is clinically challenging because it is a rare disease, which closely mimics more common pancreaticobiliary malignancies in its presentation such as obstructive jaundice and pancreatic mass. The price of misdiagnosis is high because AIP diagnosed as pancreatic cancer can lead to unnecessary surgery for the benign disease, and cancer diagnosed as AIP can delay potentially curative surgery. There is no single ideal diagnostic test for AIP; hence one has to use a set of diagnostic criteria to distinguish it from other diseases. International consensus diagnostic criteria (ICDC) and algorithm for AIP have been proposed by a consensus of expert opinion in 2011. The concept of the Japan pancreas society (JPS) 2011 criteria took basic concepts of both the Japanese previous criteria and type 1 in the ICDC as much as possible. However, the ICDC are very complex to remember and definition of level 1 and 2 are not evidence based in some criteria. The revised JPS criteria are simpler than ICDC but further evaluation is necessary in other than Japan. So, further research is required to establish easy, ideal and practical diagnostic criteria.

During January-November 2012, a total of 2,041 quarter milk samples were collected from dairy cattle of 82 dairy farms nationwide. About 42% (870/2,041) of the samples that had somatic cell counts (SCC) of ≥ 200,000 cells/ml were subjected to microbiological examination. No bacteria was isolated from 95 of 870 (10.9%) samples. Among 1,237 bacteria isolated from the rest 775 samples, 1,085 were identified with VITEK: more than half (52.1%, 645/1,237) of the isolates were gram negative bacillus. Gram positive cocci including Staphylococcus accounted for 35% of the isolates and almost none of gram positive bacilli isolated. Excluding Coagulase-negative Staphylococcus (CNS), the most frequently isolated bacterial species was Escherichia coli (11.2%, 138/1, 237), followed by Klebsiella pneumoniae (8.1%,100/1,237), Staphylococcus aureus (7.1%, 88/1, 237), Enterobacter cloacae (6.0%, 74/1, 237), and Serratia marcescens (3.5%, 43/1, 237). The most common resistance of S. aureus was to penicillin (77.4%) and ampicillin (73.0%), while no resistance was observed against gentamicin and cephalothin. Although CNS presented resistance to all antimicrobials tested but the most prevalent resistance was to penicillin (35.6%) and ampicillin (37.0%). The pattern of antimicrobial resistance observed in CNS was similar to that of S. aureus, but the rates were much lower than those of S. aureus. E. coli also showed resistance to all the antimicrobials tested, although the rates were not very high. The highest resistance of E. coli was to cephalothin (39.4%) and ampicillin (36.2%), while most of the strains (98.0%) showed sensitivity to amikacin. The results of this study provide information on current situation of bovine mastitis in Korea.

This study designed a “Diagnostic Model for Management Innovation of Medium Enterprises” based on the theoretical background of success factor and management diagnosis model for management innovation of medium enterprises and suggested a measure for utilization of strategic subject and diagnostic model that enterprises can apply. Utilization of medium enterprises management innovation diagnostic model designed through this study would be of help for making a diagnosis of the capability maturity level of enterprises’ current management system and improving it by establishing a challenging capability objective and building a circulation system capable of innovating enterprises. It is expected for enterprises to overcome growing pains and establish a management system capable of achieving outcome (productivity) by repeating measurement and innovation through management diagnosis. In addition, this study provides a method to produce a strategic subject, select priority of implementation and prepare an implementation road map by classifying and filtering management issues produced as a result of management diagnosis in a systematic way. If variables necessary for production of an objective weighted value of scoring and discover of elements for category of diagnostic model and elementary items as well as design of a self-diagnosis questionnaire, measurement of management outcome suggested in this study can be able to be verified and supplemented through case study in the future, it is expected to make the degree of completion as a diagnostic model elevated that may help for growth and development through innovation of medium enterprises.

혈액 속의 글루코우즈의 농도를 측정하기 위해 포스파젠 고분자로 만들어진 진단막을 제조하였다. 혈액 속에 존재할 가능성이 있는 장애성분들이 글루코우즈의 농도측정에 미치는 영향을 조사하였다. 그러한 물질들 중, ascorbic acid (AA)는 기준 플라즈마 용액보다 3~9% 정도 높은 K/S 결과치를 나타내었고, tolbutamide (TA)는 기준용액보다 11~13% 정 도 낮은 K/S 결과치를 나타내었고, triglycerides와 bovine serum albumin (BSA)은 기준용액 보다 20~25% 정도 낮은 K/S 결과치를 나타내었다. 그러나 위의 물질들을 제외하고는 대부분의 장애성분들이 포스파젠 고분자 진단막을 이용한 혈당측정에 큰 영향을 미치지 않는다는 것을 보여 주었다.

The purpose of this study is to identify a suitable organizational diagnostic model for analyzing the management efficiency of food service firms. We used the three-iteration Delphi technique on a panel of 19 business employees, 7 industry experts, and 4 employees in the food service industry. A total of 36 assessment indicators were developed through this panel, with numerous major findings. First, it is important to evaluate the leadership qualities of employees in terms of their motivation and competency. Second, it is important to evaluate the ability of employees to interface well with other employees. Third, it is important to evaluate and manage the brand image recognized by customers. Fourth, it is important to evaluate the fairness and regularity of the rewards given for an excellent job performance. Fifth, it is important to evaluate the level of communication and information distribution in the organizational culture. Last, but not least, it is important to evaluate the transparency and fairness of an organization in its human resource management (HRM) and the efficiency of its organizational structure. In conclusion, this study empirically shows how food service firms can develop an organizational diagnostic model to increase their managerial efficiency.

The diagnostic assay of calcium ion was sought using a modified sensor with square-wave stripping voltammetry (SWSV) and cyclic voltammetry (CV). In this study, simple graphite pencil was used as working, reference, and auxiliary electrodes. By coating the working electrodes with DNA, their sensitivity was very much improved, and good results were yielded. Moreover, clean seawater was used as an electrolyte solution instead of acid and base electrolytes to lessen the expenses involved in the experiment. The analytical optimum conditions were also examined. These conditions were attained at the low detection limit of 0.6ugL1. After that, the results were applied to drinking water of milk contain.

Pine-wilt disease (PWD) is one of the most devastating forest diseases in Eurasia. PWD is known to be caused by pine wood nematode, Bursaphelenchus xylophilus which is indigenous in North America. Ever since PWD was found in Japan, PWD has been continuously spreading to Korea, Taiwan, China, Portugal and Spain. Because B. xylophilus infected trees died within 2 ~ 3 months, the most effective way to control PWD is preventing the translocation of infected trees to other regions. Thus, developing on-site diagnostic methods for identifying B. xylophilus infected tree is utmost important. Even though various cellular and molecular biological techniques were developed to identify B. xylophilus at the laboratory, they had certain limitations to be applied for on-site diagnostic methods. In this presentation, I will overview the recent advances in B. xylophilus detection methods. And then, I will present recent progression for developing on-site diagnostic methods for B. xylophilus made from my laboratory collaboration with Korea Forest Research Institute. The development of on-site diagnosis tool for PWD is one of the most arduous mission to accomplish. Thus, the accomplishment of our mission requires continued interest and support to PWD researches.

Diagnosis with an ex-vivo gold sensor was done using a modified fluorine-doping sensor, and cyclic voltammetry (CV) redox potentials of 0.4 V anodic and -0.2 V cathodic were obtained. Both peak currents were optimized using square-wave (SW) stripping voltammetry, and an analytical working range of 10-80 ug/L SW was attained. The precision of the 10-mg/L Au was 0.765 (n=8) RSD under the optimum conditions, and the analytical detection limit approached 0.006 ug/L (S/N=3) with only a 60 sec accumulation time. The developed method was used to examine the mouse droppings for medicinal diagnosis.