Although assisted reproductive technology (ART) has been developed in many mammalian species including cows, the only embryo preservation technology that is available is cryopreservation. In the present study, small molecules were used to preserve embryos at room temperature. The basic medium for embryo preservation consisted of 1% BSA non-cryopreservation medium (BNC) instead of fetal bovine serum (FBS). To maintain survival and prevent damage during embryo storage, three candidate small molecules were selected—CHIR99021, Y-27632 and Thiazovivin—and their concentrations were optimized. Then, the embryos in the small molecule supplemented preservation medium were stored at room temperature. The viability and hatching rate of embryos stored at 10°C were greater for Y-27632-BNC and CHIR99021+Y-27632-BNC compared to BNC. However, the rate was lower for Thiazovivin-BNC compared to BNC. Although there were no surviving embryos after storage at 20°C, the viability and hatching rate of embryos significantly increased in Y-27632-BNC and CHIR99021+Y-27632-BNC compared to BNC. The mechanism by which small molecules enhance survival of embryos during storage was investigated, and expression of heat shock protein 70 was observed to increase. The findings of this work may be useful in improving ART in the agricultural field.

The CO2 separation membranes based on a graft copolymer consisting of hydrophobic poly(ethylene-alt-maleic anhydride) (PEMA) backbone and hydrophilic poly(propylene glycol) PPG side chains were fabricated by a facile one-pot process. The reaction between O-(2-aminopropyl)-O’-(2-methoxyethyl) polypropylene glycol (AMPPG) and PEMA was conducted in butanol at room-temperature. Without any post-treatment, the as-synthesized PEMA-g-PPG solution could be directly coated onto a microporous polysulfone support to fabricate thin-film composite membranes. The PEMA-g-PPG membrane exhibited high selectivity (82.6 for CO2/N2 and 26.8 for CO2/CH4) and good CO2 permeability (99.1 Barrer), which is a close value to the upper boundary limit (2008). The PEMA-g-PPG membrane could be commercially feasible owing to simple, inexpensive and scalable process.

The effects of high hydrostatic pressure (HHP) or concentration treatment or sodium hydrogen carbonate addition on microbial and quality properties of cold drip liquid coffee were investigated during storage at room temperature. Cold drip liquid coffee was determined to pH, acidity, general bacteria, and Y&M (Yeast and Molds) according to HHP treatment or adding 0.2% sodium hydrogen carbonate for 40 days storage at 20°C. Also, an equivalent mixture of concentrated coffee and cold drip liquid coffee or liquid coffee with 0.2% sodium hydrogen carbonate was used for determining the sensory evaluation, due-trio and preference test. The cold drip liquid coffee with 0.2% sodium hydrogen carbonate had higher pH and lower titratable acidity than the control during 40 days storage at 20°C. Total viable cell and Y&M counts of cold drip liquid coffee added with 0.2% sodium hydrogen carbonate or treated by HHP at 300 MPa for 25 min were lower than the control during storage at 20°C. The result of the due-trio and preference tests shows that there was no significant difference between cold drip liquid coffee added to 0.2% sodium hydrogen carbonate and the control or between the equivalent mixture of concentrated coffee with cold drip liquid coffee and the control. As a results, Cold drip liquid coffee treated by HHP, concentrated by evaporation, and added to sodium hydrogen carbonate has a potential to extend a shelf life at ambient temperature storage.

This study aimed to evaluate the fermentation characteristics of mixed Makgeolli with barley and wheats under, fermentation at different temperatures (20, 25, and 30℃) and time (3, 6, and 9 days). The pH and sugar of mixed Makgeolli were 3.68~4.26 and 12.30~17.31 ∘Brix, respectively. The total acidity showed proportionate increase with fermentation temperature and time; whereas, reducing sugar contents decreased with increasing fermentation temperature and time. The alcohol contents of mixed Makgeolli varied significantly by fermentation temperature and time (p<0.05). In terms of color values, the L value decreased with increasing fermentation temperatures, and a value increased significantly with increasing fermentation time. The number of microorganisms (yeast, total bacteria and lactic acid bacteria) in mixed Makgeolli decreased progressively with increasing fermentation temperature and time. Among the organic acids (citric, lactic, malic, and pyruvic acids) in mixed Makgeolli, citric and lactic acid was present at the highest concentrations. From these results, the optimum fermentation conditions of mixed Makgeolli with barley and wheat were 20~25℃ for 6 days.

P92 steel weldment scheduled to use for next generation ultra super critical(USC) boiler header is assessed on creep characteristics. The test method to assess local structure of weldment is small-punch creep(SP-Creep) test, which is a kind of micro test proved the availability on evaluation of mechanical property for local structure. The results for P92 steel weldment are compared with that of tensile creep test for same microstructures of steel weldment. Overall, the creep resistance of coarse grain HAZ(CGHAZ) at 650℃ is inferior to the other structures while fine grain HAZ(FGHAZ) is most superior in the P92 steel weldment. The power law relationships can be obtained for each weldment structures(BM, ICHAZ, FGHAZ, CGHAZ and W.M) of USC boiler header

This present study deals with the effect of micro-alloying elements and transformation temperature on the correlation of microstructure and tensile properties of low-carbon steels with ferrite-pearlite microstructure. Six kinds of lowcarbon steel specimens were fabricated by adding micro-alloying elements of Nb, Ti and V, and by varying isothermal transformation temperature. Ferrite grain size of the specimens containing mirco-alloying elements was smaller than that of the Base specimens because of pinning effect by the precipitates of carbonitrides at austenite grain boundaries. The pearlite interlamellar spacing and cementite thickness decreased with decreasing transformation temperature, while the pearlite volume fraction was hardly affected by micro-alloying elements and transformation temperature. The room-temperature tensile test results showed that the yield strength increased mostly with decreasing ferrite grain size and elongation was slightly improved as the ferrite grain size and pearlite interlamellar spacing decreased. All the specimens exhibited a discontinuous yielding behavior and the yield point elongation of the Nb4 and TiNbV specimens containing micro-alloying elements was larger than that of the Base specimens, presumably due to repetitive pinning and release of dislocation by the fine precipitates of carbonitrides.

2015년 10월부터 2016년 4월까지 주 1회 원주천의 조류를 관찰하여 각 수조류의 도래에 미치는 기온의 조건을 알고자 하였다. 원주천에 도래하는 수조류의 개체수는 기온의 변화와 반비례하여 기온이 낮아짐에 따라 개체수는 증가하였고, 최저기온을 보인 16차(27-Jan) 조사에서 최고개체수를 기록하였다가 기온이 상승함에 따라 감소하였다. 원주천 수조류의 전체 개체수는 인근 저수지와 하천의 결빙율에 비례하여 결빙율이 높아질수록 개체수가 증가하였고 낮아질수록 감소하였다. 기온변화에 따른 종별 개체수의 상관성을 알아보기 위하여 1차(15-Oct)에서 27차(18-Apr)에 이르는 월동기 전체의 상관관계(1~27상관)를 비롯하여 최저기온을 기록한 16차를 기준으로 1차(15-Oct)에서 16차 (27-Jan)의 월동 전반기의 상관관계(1~16상관), 16차(27-Jan)에서 27차(18-Apr)에 이르는 월동 후반기의 상관관계 (16~27상관)를 알아보았다. 대부분 월동 수조류는 기온에 대해 음의 상관성을 보였으나 쇠백로(Egretta garzetta), 민물가마우지(Phalacrocorax carbo) 및 왜가리(Ardea cinerea)는 양의 상관성을 보였다. 흰뺨검둥오리(Anas poecilorhyncha)가 기온의 변화에 대한 가장 높은 상관성(1-16상관, 7D/H3A, (r=-0.960, p<0.000000001)을 보였으며, 전체 월동 수조류는 모든 상관분석(1~27상관(r=-0.942, p<0.01), 1~16상관(r=-0.947, p<0.01), 16~27상관(r=-0.958, p<0.01))에서 4D/LA에 가장 높은 상관성을 보였다. 각각의 수조류와 기온에 대한 상관성은 종마다 기온(AT, HT, LT)과 기간(1D~7D)의 조건에 대해 각각 다르게 나타났다. 대부분의 종이 최고기온보다는 최저기온과의 상관성이 큰 것으로 나타났으며, 기온이 하강하는 월동 전반기에는 4일~7일의 기온에, 월동 후반기에는 1일~3일의 기온에 높은 상관성을 보였다.

본 연구는 전북권내 냉장·냉동식품 유통차량을 대상으로 온도관리에 대한 현황을 파악하고자 중소기업 2곳과 대기업 5곳의 운송차량 총 8대를 대상으로 온도현황을 조사하였다. 냉장·냉동식품 유통차량의 실제공간상의 온도분 포 조사결과, 참여 중소기업의 냉장차량과 냉동차량 평균 온도는 각각 8.35 ± 5.72oC, −3.45 ± 16.88oC이었고, 대기 업의 냉장차량과 냉동차량 평균온도는 각각 3.92 ± 1.44oC, −15.38 ± 2.98oC로 중소기업 보다는 낮은 온도로 운영되는 것으로 나타났다. 냉장식품 적재함의 공간위치별 온도 차이는 평균적으로 2.40oC ± 1.45oC, 냉동식품 적재함은 2.37oC ± 2.52oC의 차이가 나타났지만 공간위치별 냉장, 냉동온도 모두 통계적으로 유의하지 않았다(p > 0.05). 냉장·냉동식품 하역에 따른 유통차량 문 개·폐 전·후의 식품 표면온 도는 냉장식품의 경우 열린 직후 평균 2.17 ± 1.90oC에서 닫힌 직후 2.72 ± 2.36oC로 약 0.55oC 상승하였으며, 냉동 식품은 열린 직후 −18.58 ± 3.66oC에서 닫힌 직후 −17.76 ± 4.15oC로 약 1.18oC의 온도 상승이 나타났다. 본 연구결과 냉장·냉동식품 유통차량의 공간위치별, 이동 시간별, 그리 고 유통차량 문의 개·폐에 따라 다양한 온도변화가 나타 날 수 있음이 확인되었다. 따라서 유통 및 이동과정 중 온도변화를 실시간으로 확인할 수 있는 온도이력(Time- Temperature History) 추적시스템 도입에 대한 현장 적용 연구가 수행되어야 할 것으로 생각된다.

급격한 수온의 변화는 어류의 생리학적인 측면에서 스트레스를 유발한다. 본 연구에서는 넙치 (Paralichthys olivaceus)로부터 각 수온별(9, 12, 15, 18 및 21℃) 조건에 따라 24 및 48시간 동 안 노출시킨 후에, 혈액생리학적 분석, 스트레스 단백질로 알려진 Hsp70 mRNA 발현 및 산소 소비량을 조사하였다. 혈액학적 분석에서 hematocrit (Ht) 및 hemoglobin (Hb), 혈장 코티졸 및 글루코스의 변화, aspartate aminotransferase (AST) 및 alanine aminotransferase (ALT), NH3, 삼 투질농도(osmolality) 및 총단백질(total protein, TP)은 9℃ 및 12℃에서 다른 수온별 실험구에 비 해 대부분의 항목에서 유의적인 차이를 보였다. Hsp70 mRNA 발현은 9℃ 및 12℃에서 다른 실험구에 비해 높은 발현량을 확인하였고, 산소소비량은 9℃ 및 12℃에서 21℃에 비해 낮았다. 이러한 결과는 넙치 종자의 장거리 수송을 위한 수온자료로 활용할 수 있다

Among the Fire Protection in the interior representative case are spray coat, board, Paint. Intumescent paint has been developed that price competitiveness manufactures and Fire-resistances certificate was verified by experiment. Recent three-hour fire resistance performance developed Intumescent paint is applied as a high-rise, and major structures designed according to the refractory needs to be more efficient. However, domestic and outside the thermal properties of Intumescent paint materials are not proposed. Meanwhile, Intumescent paint in fire is built char layer since the 500∼60 0℃ and middle layer is formed by the decuple intumescent char more then cover thickness at room temperature. It is cut temperature flow off to the steel surface. Nonetheless, Bond Strength of steel and paint deprive as the intumescent char increases. and then steel surface are exposed to flame instantaneously. In this study, intumescent char estimate according to structural parameter(Fire resistance, cover thickness, shape ratio).

온도 및 습도에 민감한 화물에 대한 수요가 증가하고 있다. 이에 우리나라도 항만에서 온도 및 습도에 민감한 화물을 적극적으로 유치할 필요가 있다. 따라서 본 연구에서는 온도 및 습도 민감화물 유치에 대한 해외사례를 분석하여 시사점을 도출하고 우리나라에 유치 가 능한 품목을 선정하는데 목적이 있다.

This study is a development of measuring instrument capable of measuring the temperature measurement at a temperature of between 0 ℃ and 1600 ℃ at a high temperature of the high temperature. This development technique develops a measuring device capable of measuring two different temperature measurements by combining two different temperature measurements ranging from one measurement module to the hot temperature to the high temperature. Also, it develops in a vacuum or high pressure state.

To assess the temperature change effects on Collembola communities, we selected 9 Korean mountains in different latitudes. Top soil was collected and Collembola extracted from 3 sites in different elevation in each mountain. Extracted Collembola were sorted to genera and this data were summarized as total abundance, family richness and diversity. Additionally, soil temperature in each plot, physical and chemical properties of the soil was investigated and that correlated with Collembola community data. Average soil temperature and soil properties affected patterns in Collembola abundance and family richness. The results indicate that temperature change and soil properties strongly affected Collembola communities. Therefore, Climate change has high potential to affect Collembola communities.

Pseudococcus orchidicola and Pseudococcus longispinus, a notorious cosmopolitan pest species of mealybugs, known to be distributed indoors in Korea. The effect of temperature on the development of P. orchidicola and P. longispinus was studied. The duration of nymphal period of the two mealybugs at 24 and 28℃ have been recorded. Developmental duration of P. longispinus was longer than P. orchidicola in both females and males. The adult longevity was estimated to 74.9 and 91.1 days at 24℃ for P. orchidicola and P. longispinus, respectively. The average fecundity per female was greater at 28℃. The survival of P. orchidicola and P. longispinus during winter at outdoor was failed.

The soybean bug, Riptortus pedestris, is a polyphagous species and an important pest of soybean. We investigated the development periods and survivorship of R. pedestris egg and nymph stage. The study was conducted at eleven constant temperatures of 12, 14, 16, 18, 20, 24, 28, 32, 34, 35, and 36℃, respectively. Egg could not hatch under 14℃. Nymph could not develop to adult at 16℃. The lower developmental threshold (LDT) and thermal constant (K) of R. pedestris nymph stage were estimated by linear regression. Higher temperature threshold (TH) and lower temperature threshold (TL) were calculated by two nonlinear functions (Performance and SSI model). The adult emergence frequency of R. pedestris over full range of constant temperatures was simulated using developmental rate functions and Weibull function.

The pea aphid Acyrthosiphom pisum (Harris) (Hemiptera: Aphididae) is a pestiferous species with a worldwide distribution. We investigated the development periods and survivorship of A. pisum nymph, and longevity and fecundity of A. pisum adult. The study was conducted at five constant temperatures of 10, 15, 20, 25, and 30°C. Higher temperature threshold (TH) and lower temperature threshold (TL) were calculated by two nonlinear functions. The temperature range of A. pisum from TL to TH using empirical model (29.29) was wider than that using biophysiological model (21.85). The adult emergence frequency of A. pisum over full range of constant temperatures was simulated using developmental rat

Effects of temperature on development and ovipistion of Amblyseius eharai, were examined at 11 constant temperatures (18.0, 20.1, 21.6, 24.0, 24.1, 27.4, 28.6, 30.2, 32.0, 33.2 and 35.9 °C), and six constant temperatures (18.0, 21.6, 24.1, 27.4, 30.2 and 33.2 °C) by using Tetranychus urticae as prey. Developmental rates were fitted with a nonlinear Briere 1 model, which estimated optimal temperatures for the development as 30.6 °C for total immature stage. In a linear model, the lower thresholds and thermal requirements (DD) were 13.2 °C (71.94 DD) for total immature stage. Fecundity was well described with the Extreme value function, which indicated optimal temperatures for the oviposition as 24.3 °C. Overall, findings herein provide comprehensive data and explanations on the temperature-dependent development and oviposition of A. eharai, and prove the probability of A. eharai as a good biological agent for T. urticae in early or late crop seasons, and/or at low temperature cultivation farm lands.