During the survey of plant parasitic nematode of Citrus orchard in Korea, Paratrichodorus minor (Colbran, 1956) Siddiqi is founded from the soil of Citrus unshiu Markovich for the first time in Korea. The body length of females is 428.34~506.22 ㎛ long: a = 12.08~22.79, b = 4.02~5.02, V(%) = 38.46~60.68. The excretory pore is located near the base of esophagus. Additionally, the morphological characteristics on P. porosus (Allen, 1957) Siddiqi, 1974 recorded in 1976 on Korea is redescribed in detail.

Survival rate and development of the two spotted spider mite, Tetranychus urticae are decreased by UV-B irradiation (Otsuka and Osakabe, submitted). This is considered to be a reason why this mite prefers lower leaf surfaces host plants. On the contrary, the citrus red mite, Panonychus citri is known to remain on upper leaf surfaces of host plants. Such difference in the distribution was assumed to depend on the difference in UV resistance. Hatchability of P. citri eggs was decreased by UV-B irradiation, yet their eggs were more protected from the UV-B irradiation in company with T. urticae. Eggs of P. citri are reddish, while those of T. urticae are white. To test whether the reddish color invest UV resistance in eggs, pale white eggs of albino strain and reddish eggs of wild type of P. citri were simultaneously irradiated by UV-B. Reddish eggs of wild type showed higher resistant to the UV-B irradiation than the pale-white eggs of albino strain. For the next step, the wild type and the albino eggs of P. citri under UV opaque film (-UV) or under UV transparent film (+UV) were exposed to solar irradiation. Hatchability of albino eggs was signifycantly lower than that of wild type eggs under the + UV condition. Under UV –, both eggs showed higher hatchability than under UV+ conditions. From these results, the wild type eggs of P. citri were likely to have some mechanism protecting itself from ambient UV irradiation, and the red pigment possibly act as a shield againt solar UV radiation. F1 female proceeded by reciprocal crosses between the wild type and the albino strain exhibited reddish body color in their adult stage, indicating that the albino was recessive. Notwithstanding, F1 eggs from the crosses between albino female and wild type male were pale white. After hatching, the F1 larvae also exhibit pale white body color, then the females turned to reddish at their first quiescent stage. F1 progeny from the crosses between wild type female and albino male were all reddish during egg to adult stage. These revealed that the color of eggs and also larvae were conferred as a maternal effect (: eggs of P. citri might be protected by the red pigments as the first gift of their mother.)

Neoseiulus californicus (McGregor) is a potential biological control agent of spider mites in orchards. The field occurrence of this species was first reported from citrus orchards in Jeju Island and recently this mite started to be sold as a commercial product in Korea. However, the natural occurrence of N. californicus in the Korean mainland is unclear or quite limited. At this time, it is not certain whether N. californicus can overwinter in the Korean mainland or not. This mite is presently planning to be used as a biological control agent against spider mites in apple orchards of the Korean mainland. The main objective of this study was to investigate natural overwintering sites of N. californicus and possible factor increasing winter survival of this species, to develop effective method for collecting overwintering population of N. californicus. For the first objective, we conducted field survey in two citrus orchards of Jeju in early and late February 2008. Samples were collected from various possible overwintering sites: citrus trees, ground covers and windbreaks. Total 259 phytoseiid mites were collected, 94% of the mites were identified as N. californicus and 98% of them were adult females. Most of N. californicus were collected from ground covers, specially the weed species which had rosette leaves: Youngia japonica (L.) DC. and Erigeron spp. This result seemed to suggest that N. californicus try to hide beneath weed leaves near ground surface during winter to get successful survival. Thus, the second experiments were conducted in Andong from December 2007 to early March 2008. N. californicus conditioned in a greenhouse with natural day length was individually put in a small tube, and the tubes were kept under different ground surface conditions: bare, fallen leaves and urethane foam. When water had been added in the tube, the mites on bare ground had died after one month, while 70% of the mites of the other cover treatments were surviving. In these cover treatments, 7-10% of the mites had survived until two months, and eventually all died within three months. This result showed that hiding under cover structure near ground surface may be effective to increase winter survival of N. californicus. Therefore, collecting weeds which have rosette leaves may be an effective method to estimate of the overwintering possibility of N. californicus in the Korean mainland.

In Jeju cirtrus orchards, Panonychus citri, citrus red mite is the most important pest requiring 3 times acaricide sprays. In open field conventional orchards, P. citri usually shows three population peaks; from end of Jun to July, from end of Aug. to Sep., from end of Oct. to Nov. However, natural enemy complex and its function regulating P.citri are poorly understood. From the survey of P.citri natural enemy in citrus orchard in Jeju from 2004 to 2006, predatory beetle, Oligota spp. was most abundant. Three predatory mite, N. californicus, Amblyseius eharai, and N. barkeri, were identified. Among them, the predatory mite, Neoseiulus californicus (McGregor) (Acari: Phytoseiidae), was first found in Korea. Even though it was first found, N. californicus was the dominant species occupying 84% of phytoseiid mites. These predatory mites mostly occurred in Jun and peaked at July, which was accorded with the high humidity season of the year. From the survey, the density relationship with P. citri was unclear. From the study conducted in 2005-2007, N. californicus was more abundant in greenhouse citrus (var. Shirahuhi) than in open field orchards; conventional or organic mandarine citrus. In greenhouse citrus, phytoseiid mites showed suppressing P. citri population. As a next step, the inundative biological control study was conducted using commercial strain of N. californicus, which was originated from Jeju, 2005, in greenhouse citrus. One thousand N. californicus per 1a were released 2 times at 10 day interval on citrus leaves when the initial density of P. citri was about 0.2 per leaf. The release effects were variable depending on the field condition. However, N. californicus did successfully reduce P. citri in greenhouse citrus orchards.

Cryptic mealybug (CMB), Pseudococcus cryptus, is a serious scale pest on citrus, especially grown in plastic greenhouse on Jeju island, Korea. Binomial sampling plan was developed using the relationship between infested proportion of twig with less than T scales (pT) and mean density per twig (m) according to the empirical model ln(m)=α+βln(-ln(1-pT)). T was defined as tally threshold and set to 1, 2, 3, 4 and 5 scales per twig in this study. T=3 was the best tally threshold for estimating densities of CMB based on the precision of the model. Binomial model with T=3 provided reliable predictions of mean densities of CMB observed on commercial citrus orchards. The obtaining model was evaluated by resampling validation of Wald's sequential binomial plan and by fit at 95% confidence intervals for independent collected data sets. Binomial sampling program for decision-making of CMB population level was obtained at the action threshold 0.10, proportion of twig infested with more than 3 scales.

To find effective native natural enemies of spider mites in the surrounding vegetation of citrus orchards, we investigated the species composition and seasonal occurrence of phytoseiid mites on Podocarpus macrophyllus, which is one of the major windbreak tree species surrounding citrus orchards in Japan, in Nagasaki prefecture, south-western Japan, from 2006 to 2007. Six species were found: Amblyseius eharai, Euseius sojaensis, Okiseius subtropicus, Typhlodromus vulgaris, T. kishimotoi, and Typhlodromus sp. Amblyseius eharai was the most dominant species and formed more than 60% of the phytoseiid species collected in both years. Typhlodromus vulgaris was the second most dominant species. The other species were rare, except that Okiseius subtropicus was abundant in February in 2006. The density of A. eharai showed two peaks in late June and from late August to early September. The density of T. vulgaris also peaked in late June. However, the density declined thereafter, and remained low, unlike the seasonal occurrence of A. eharai. Podocarpus macrophyllus blooms from early to mid-June and produces a great deal of pollen. Both A. eharai and T. vulgaris developed and oviposited well when they were reared on pollen of Podocarpus macrophyllus as food. Therefore, the increase of these phytoseiid species in June was thought to be due to the supply of pollen of Podocarpus macrophyllus as food.

본 연구 결과 동결건조된 감귤과피 70% 메탄올 추출물의 총 폴리페놀 함량은 836.8 ㎎%였으며 감귤과피 추출물 0.01, 0.02와 0.1%의 EDA는 각각 89.6, 86, 81.3%로 높았으며 아질산염 소거능은 pH 1.2에서 34.4%, pH 7.0에서 19.5%를 나타내어 산성 영역에서 높은 소거작용을 보였다. 또한 이들 추출물의 항산화효과는 옥배유의 경우 TBHQ 〉 BHT 〉 TOC 〉 ChEx 〉 EaEx 〉 EtEx 〉 WaEx 〉 Control 순이었고, 채종유의 경우 TBHQ 〉 ChEx 〉 EaEx 〉 BHT 〉 EtEx 〉 WaEx 〉 TOC 〉 Control 순이었다. 두 유지모두에서 에틸아세이트추출물(EaEx)과 클로로포름추출물(ChEx)이 에탄올추출물(EtEx)과 물추출물(WaEx) 보다 항산화 효과가 높았으며 첨가농도가 증가함에 따라 항산화 효과도 증가하는 경향을 보였다. 그리고 감귤과피의 각 용매별 추출물의 공액이중산가도 에틸아세테이트추출물(EaEx)과 클로로포름추출물(ChEx)이 높았으며 이들의 항산화 효과는 TBHQ보다는 낮았으나 BHT, tocopherol과 비교하였을 때 거의 유사하거나 좀 더 높은 경향을 보였다. 한편 감귤과피 추출물은 우수한 항균력을 보여주었고 농도가 상승함에 따라 항균효과도 상승하였으며 이 효과는 그램 양성, 음성균주에서 고르게 나타나 천연 항균소재로서의 이용가능성을 확인하였다. 이상의 결과에서 감귤 과피는 총 폴리페놀 함량과 전자공여능 활성이 높아 항산화효과와 항균효과가 우수하게 나타나 감귤 과피의 기능성 이용도가 매우 높을 것으로 생각되며 감귤 가공 후 생기는 부산물들의 식품산업에서의 유용성도 높을 것으로 사료된다.

The citrus leafminer (CLM), Phyllocnistis citrella Stainton, is an oligophagous pest of Rutaceae family, especially Citrus spp. occurring in most worldwide citrus-growing areas. This study was conducted to evaluate a sex pheromone chemical of CLM, (Z,Z)-7,1l-hexadecadienal (7Z,11Z-16:AI) in monitoring CLM by trap types, the diel activity and the influence of some weather factors on trap catch. CLM was well attracted on a trap baited 7Z,11Z-16:AI 1㎎. Sticky wing trap was more effective than bucket trap. Most caught CLM were attracted at 2~6 a.m. regardless of season, and activity time of CLM was affected by sunrise time as well as sunset time. The trap catch of CLM was more influenced by wind velocity than temperature for activity time of CLM. The number of caught CLM was fallen at below 13℃, but there was little effect for trap catch at over that temperature. The average wind velocity at over 2.0 m/sec made the number of caught CLM drop down. The precipitation did not affect the number of caught CLM when the average wind velocity was lower than at 2.0 m/sec.

Predation of Neoseiulus fallacis was observed for biological control of Panonychus citri that is one of the major insect pests on citrus. The daily predation of development stages of P. citri by an adult female of N. fallacis were 20.1 eggs, 26.1 larvae, 18.2 protonymphs, and 7.4 deutonymphs at 25℃. The daily predation of P. citri eggs by N. fallacis was observed under different temperatures. The predation was increased as the temperature rise. At this time, ratio of eggs production of N fallacis after predation of P. citri eggs (number of eggs N. fallacis/number of eggs P. citri consumed by N. fallacis) was 0.09. The daily predation of P. citri eggs by N. fallacis was 21.1, 17.3, and 16.7 on the different arenas (diameter: 20, 40, and 60 ㎜), respectively. The predation was decreased as the arena of the leaf increase. The functional response of N. fallacis to P. citri showed Holling"s Type Ⅱ response: the consumption of prey by N. fallacis increased as the density of prey increase but increasing rate was gradually reduced. As the result, it seemed that N. fallacis can be use for biological control of P. citri.

본 연구는 투과증발법을 이용하여 수용액 중 미량의 감귤 향 성분을 농축하는 방법으로, 4종류의 실록산계 고분자 복합막을 이용하여 막 종류와 구조에 따른 투과 특성을 살펴보았다. 또한 최적의 막을 선정하여 공급액의 온도와 농도, 순환 유속에 따른 투과 특성을 살펴보고, 이를 resistance-in series model을 이용하여 해석하였다. 4종류의 실록산계 고분자 복합막을 통한 감귤 essence aroma 모델액의 투과 실험에서 지지층이 polyvinylidene fluoride (PVDF)이고 활성층이 polyoctylmethyl siloxane (POMS)인 막이 가장 높은 향 성분 플럭스와 농축계수 값을 나타내었으며, 공급액의 온도와 농도, 순환유속을 변화시키며 투과 실험을 하였다. 그 결과 공급액의 온도와 농도가 증가됨에 따라 향성분의 플럭스는 증가하고 농축계수 값은 감소하였으며 순환유속이 증가됨에 따라 향성분의 플럭스와 농축계수 값 모두 증가하였다.

길이 40m,폭 5.5m의 단일피복 구조의 8연동 무가온하우스 상단부에 설계적설심 19.1 cm의 눈이 쌓인다는 조건과 시설 측면으로 설계풍속 36.6 m·s-1의 바람이 분다는 조건 그리고 참고자료로 활용하기 위해 적용한 최대적설심 37.8cm의 눈이 쌓인다는 조건과 순간최대풍속 60.0 m·s-1의 강풍이 분다는 조건에서 유동 및 구조강도 해석을 수행하였다. 적설하중 조건에서는 설계적설심 19.1 cm와 최대적설심 37.8cm에서 파이프에 걸리는 최대응력이 각각 53.8 N·mm-2과 107 N·mm-2으로 재료의 허용응력 보다 작은 것으로 나타나 안전한 것으로 분석되었으나, 설계풍속 36.6 m·s-1와 순간최대풍속 60.0 m·s-1의 풍하중 조건에서는 파이프에 걸리는 최대응력이 각각 250 N·mm-2과 672 N·mm-2으로 재료의 허용응력을 모두 초과하여 플라스틱하우스가 불안전한 것으로 분석되었다.