양송이배지로부터 분리한 인산가용화균 Paraburkholderia phenazinium YH3와 Paraburkholderia metrosideri YH4의 단일접종 및 동시접종에 따른 인산가용화 능력의 시너지효과 및 상추생육효과를 조사하였다. 인산캄슘이 함유된 NBRIP 액체배지에 분리균을 접종하고 해리된 가용인산 (soluble phosphorus)함량을 HPLC에 의해 분석하여 인산가용 화능을 측정한 결과, 배양 3일차에 동시접종구 1250 μg mL -1 , P. metrosideri 1196.59 μg mL -1 , P. phenazinium 994.34 μg mL -1 의 순으로 나타나 두 종의 인산가용화 박테리아간의 동시 접종에 의한 시너지효과가 관찰되었다. 배지 내 pH 와 잔류 glucose 함량 변화도 모든 접종구에서 배양 1일 후 대부분의 변화가 이루어져 pH의 경우 초기 pH 7.0에서 pH 4.0 수준으로 감소하였으며, 잔류 glucose 함량은 초기 10 mg mL -1 에서 4.8 mg mL -1 수준으로 검출되어 인산가용 화능과 거의 유사한 경향을 보였다. 배양여액의 유기산 분석결과 gluconic acid 약 11 mg mL -1 와 malic acid 약 4 mg mL -1 수준으로 가장 높게 검출되었으며, Park etal(2016)에 의해 보고된 oxalic acid는 검출되지 않았다. 대부분의 유기산이 배양 1일과 3일 후 생성되는 결과를 보임으로서 유기산이 배지의 pH를 감소시키고 인산 가용화를 유도하는 주요 원인임을 확인할 수 있었다. 접종 4주 후 상추 생육차이를 조사한 결과, TCP를 첨가하지 않은 P. phenazinium 접종구, P. metrosideri 접종구와 동시 접종구에 비해 TCA 첨가한 모든 처리구에서 10 -20% 수준의 생육증진효과가 확인되었으나 단일접종과 동시접종 구간에 유의수준의 차이를 확인할 수 없었다. 결과적으로 인(P) 결핍 토양 및 염류토양에 인산가용화균을 사용하면 식물과 작물 수확량에 의한 P 섭취가 동시에 증가하고 생육이 촉진된다는 다른 연구결과(Rodríguez et al, 1999; Suh et al, 2008; Walpola and Yoon. 2013)와 유사한 경향을 보였다.

본 연구는 특허출원이 기업 성과에 영향을 미친다는 가정 하에 중소중견 기업을 위한 산업별 맞춤형 특허 활동성 제고 전략 5가지를 제안함으로서 향후 중소 중견기업의 특허활동 성과 제고에 기여하고자 하였다. 연구대상은 특허 활동이 활발한 25개 산업을 대상으로 2010년부터 5년간의 특허 활동성 통계자료와 2014년도 기준 기업성과 통계자료를 활용하였다. 연구절차는 DEA-BCC 효율성 분석과 특허 활동성 분석, 그리고 특허 포트폴리오 분석을 각각 실시한 후, 이를 종합한 특허 활동성 제고 전략을 제안하는 순으로 진행하였다. 연구 결과, 특허 활동성이 높고 IRS(규모 수익체증)형태를 보인 5개 산업의 경우, 특허 효율성이 높은 산업으로서 기업성과 향상에 실질적인 도움이 되고 있음을 확인하였다, 반면, 특허 활동성은 높으나 규모수익 이 IRS(규모수익체중)/DRS(규모수익체감)형태로 나타난 12개 산업을 비롯, 특허 활동성도 낮고 규모수익이 DRS(규모수익체감)형태, CRS/IRS 혼합형태, 그리고 IRS 형태 로 나타난 8개 산업 모두, 산업별 특성에 따라 특허 효율성도 다양한 형태로 나타날 수 있음을 확인하였다.

본 연구는 밀폐형 식물생산시스템에서 인공광원과 배지 종류에 따른 상추의 생육 특성을 조사하기 위해 수행되었다. 상추 종자는 5종류의 배지인 urethane sponge(US), rockwool(RW), Q plug(QP), TP-S2(TP)와 PU-7B(PU)가 충진된 128구 플러그 트레이에 파종하였다. 상추 종자의 발아율은 파종 후 12일까지 조사하였다. 상추 묘는 파종 후 13일째에 재순환 담액식 수경재배 시스템을 이용하여 EC 2.0dS·m-1, pH 6.5와 온도 25±1oC인 밀폐형 식물생산시스템에 정식하였다. 광원은 형광등과 RB LEDs(red:blue=7:3)를 이용하여 광주기 14/10(명기/암기), 광도 150±10μmol·m-2·s-1 PPFD로 설정했다. 상추의 초기 발아율은 TP에서 가장 높았다. 최종 발아율과 평균 발아 수는 RW, QP 및 TP 배지에서 유의적으로 높았다. 초장, 엽장, 엽폭, 엽면적, 지상부의 생체중, 건물중 모두 RB LED의 QP에서 유의성 있게 높은 값을 나타냈다. 엽수, 지하부의 생체중과 건물과 SPAD는 RB LED의 QP와 TP에서 가장 좋았고, 근장은 RB LED의 TP에서 가장 길었다. 따라서 밀폐형 식물생산시스템에서 RB LED가 상추의 생육이 우수하였으며, QP와 TP가 상추의 발아율과 생육에 효과적인 것으로 나타났다. 뿐만 아니라, 밀폐형 식물생산시스템에서 상추 생산 시 신개발 배지인 TP 배지의 적용가능성을 확인하였다.

Small and Medium-sized Enterprises (SMEs) are being faced with rapid changes in their business environments due to evolution of technologies and innovation in societal eco-systems. Particularly, dynamic interactions between such environments and enterprise activities have become significant, so technology planning, which is a process of identifying appropriate directions regarding product and technology development, has received much attention to cope with such dynamics proactively. However, SMEs typically have limits in performing independent, strategical and systematical technology planning activities due to the lack of human, material and financial resources. This paper proposes the development of a product roadmapping method so that SMEs carry out efficient technology planning activities with interconnections of external business environments. The present work provides product roadmap templates that directly accommodate the influence of business environments on the product’s system and its associated super/sub-systems with the use of external environment analysis techniques including TRIZ methodology, PEST and 5Forces analysis. These templates are useful to efficiently forecast the directions of product’s development and evolution, which arise from changes in external environments. Consequently, the present work enables SMEs to flexibly cope with the era of the next R&D generation, which pursues value creation through mutual interconnection between business environments and technology development.

This experiment was conducted to investigate the effects of pH on the mineral nutrient uptake and growth of the four Chinese chives species. The Chinese chives species used in the experiment were the cultivated species grown in the farm (cultivated Allium tuberosum) and three wild species of wild Allium tuberosum, A. thunbergii and A. senescens. The pH levels of soil medium were set to be 4.5, 6.5, and 7.5. Fresh weight (FW) of cultivated A. tuberosum was highest at all pH levels. The increase of soil pH increased the FW of the wild A. tuberosum and A. thunbergii, but no difference was noted for the A. tuberosum and A. senescens. Plant height was higher in the order of wild A. tuberosum, A. thunbergii, and cultivated A. tuberosum and A. thunbergii. Notably plant height of the wild A. tuberosum increased significantly by the pH increase. The Zn content of the wild A. tuberosum was shown to be significantly higher than that of the other species and increased with the increase of soil pH. This indicates that there is a close relationship between the plant height and Zn content in Chinese chives plant. Principal component analysis for characterizing closely related A. species using the factors of plant growth and amounts of nutrients uptake showed that the cultivated A. and wild A. tuberosum were in the 4th quadrant of the graph which are classified as the same species, while A. senescens and thunbergii was in 1st and 3rd quadrant indicating different species, respectively.

β-mannanase from Trichoderma reesei was purified by DEAE Sephadex A-50 ion exchange chromatography and Sephadex G-100 gel chromatography. The molecular weight was determined to be 54 kDa by tricine SDS-PAGE. Trigonella foenum-graecum galactomannan was hydrolyzed by the purified β-mannanase, and then the hydrolysates were separated by Bio-Gel P2 chromatography. The main hydrolysates were composed of D.P. (degree of polymerization) 2, 3, and 4, 6 galactomanno-oligosaccharides. To investigate the effects of Trigonella foenum-graecum galactomanno- oligosaccharides on in vitro growth of L. plantarum, were cultivated individually on a modified-MRS medium containing carbon sources such as low- and high-molecular-weight galactomanno-oligosaccharide. Lactobacillus plantarum grew 1.8-fold after treatment with high- and low-molecular-weight galactomanno-oligosaccharides, compared to 1.3-fold for those with standard MRS medium. Bacteriocin was purified by Sephadex G-100 gel chromatography and determined to be 122 kDa by tricine SDS-PAGE. The bacteriocin activated doubly more effectively after treatment with galactosmanno-oligosaccharides compared to those with standard MRS medium. Bacteriocin showed antimicrobial activity against Staphylococcus aureus. The inhibitory compound lost activity when heated to temperatures greater than 30oC and when inhibited to pH changes that lowered the pH below 4 or raised it above 5. Furthermore, its effects were inhibited by treatment with proteolytic enzymes.

The osmolarity of a medium that is commonly used for in vitro culture (IVC) of oocytes and embryos is lower than that of oviductal fluid in pigs. In vivo oocytes and embryos can resist high osmolarities to some extent due to the presence of organic osmolytes such as glycine and alanine. These amino acids act as a protective shield to maintain the shape and viability in high osmotic environments. The aim of this study was to determine the effects of glycine or/and alanine in medium with two different osmolarities (280 and 320 mOsm) during IVC on embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) in pigs. To this end, IVC was divided into two stages; the 0-2 and 3-7 days of IVC. In each stage, embryos were cultured in medium with 280, 320, or 360 mOsm and their combinations with or without glycine or/and alanine according to the experimental design. Treatment groups were termed as, for example, "T(osmolarity of a medium used in 0-2 days of IVC)-(osmolarity of a medium used in 3-7 days of IVC)" T280-280 was served as control. When PA embryos were cultured in medium with various osmolarities, T320-280 showed a significantly higher blastocyst formation (29.0%) than control (22.2%) and T360-360 groups (6.9%). Glycine treatment in T320-280 significantly increased blastocyst formation (50.4%) compared to T320-280 only (36.5%) while no synergistic was observed after treatment with glycine and alanine together in T320-280 (45.7%). In contrast to PA embryonic development, the stimulating effect by the culture in T320-280 was not observed in SCNT blastocyst development (27.6% and 23.7% in T280-280 and T320-280, respectively) whereas the number of inner cell mass cells was significantly increased in T320-280 (6.1 cells vs. 9.6 cells). Glycine treatment significantly improved blastocyst formation of SCNT embryos in both T280-280 (27.6% vs. 38.0%) and T320-280 (23.7% vs. 35.3%). Our results demonstrate that IVC in T320-280 and treatment with glycine improves blastocyst formation of PA and SCNT embryos in pigs.

High quality and timely assessment feedback is central to student learning in higher education; however, written feedback has many limitations. One of the innovative approaches to delivering feedback to EFL learners is individualized audio-visual feedback (AVF) using screencast technology. Previous research on AVF has been extensively descriptive and mostly focused on student preferences for feedback and evaluation of various screencast software. The present study employed a mixedmethod design using pre-post writing tasks and pre-post questionnaires to investigate what particularly beneficial affordances this type of media-rich feedback might offer for writers in the English-Medium Instruction (EMI) classroom, to identify the effects of AVF on changes in learners’ motivation, and to explore students’ perceptions towards screencast feedback. The results suggest that AVF is positively received by EFL learners and that simultaneous visual cues and detailed explanations promote better understanding, engagement, and active listening. In addition, AVF significantly improves learners’ writing performance and academic motivation. The paper concludes with practical implications and suggestions for further research.

봉지재배용 표고의 적합한 배지를 개발하고자 본 연구를 수행한 결과 참나무톱밥+미송톱밥+미강(40:40:20, v/v) 혼합배지인 T5에서 pH 5.3으로 대조구와 비슷하였으며 C/N율은 71.8로 대조구 85.2 대비 낮은 경향이었다. 배양특성은 T5에서 대조구 대비 산조701호는 13일, 농진고는 18일 배양기간이 단축되었다. 자실체 특성조사 결과 산조701호 품종에서는 T5에서 대조구 대비 갓이 크고 두꺼웠으며, 대도 길고 두꺼워 개체중이 높게 나타났다. 반면 농진고 품종에서는 대조구와 비슷한 경향이었다. 배지 조성에 따른 혼합배지별 수량은 T5에서 대조구 대비 산조701호는 17%, 농진고는 19% 증수되었다. 이러한 연구결과를 바탕으로 참나무톱밥의 50%를 미송톱밥으로 대체 할 경우 배양기간이 단축되고 수량이 증수되며 또한 생산비 절감이 가능할 것이라 판단되었다.

우리나라는 이미 고령시대에 접어들어 어느 때보다 은퇴 이후의 삶이 중요하게 되었다. 본 연구의 목적은 그동안 은퇴준비와 관련하여 연구되지 않았던 현 직장에서의 직무만족도와의 관련성을 규명하고, 이들 관계에서 노후생활인식이 매개변수로 작용 하여 직무만족도에 영향을 미치는 구조를 검증하고자 하는 것이다. 본 연구는 문헌적 연구를 통한 실증적 연구를 시행하였으며, 공무원을 대상으로 은퇴준비의 하위변인인 재정·주거준비, 경제활동준비, 건강관리 등이 직무만족도에 어떠한 영향을 미치는지와 노후생활인식의 매개역할을 검증하고자 했다. 이에 따라 독립변수를 은퇴준비, 종속변수를 직무만족도, 매개변수를 노후생활인식으로 각각 설정한 후, 그 하위변인을 구성하여 서로 간의 영향 관계를 분석하였다. 연구결과, 은퇴준비, 노후생활인식, 직무만족도 간의 영향관계에서 은퇴준비는 노후 생활인식과 직무만족도에 영향을 미치는 것으로 나타났으며, 특히, 노후생활인식은 은 퇴준비와 직무만족도와의 영향 관계에서 매개역할을 하는 것으로 검증되었다.

Recent research on stem cell conditioned medium (CM) has been revealed that CM could influence on the embryo development when supplemented to in vitro culture medium. However, the optimal basal medium for CM production has not determined although it is the fundamental factor of CM. The purpose of this study is to examine the effect of human derived adipose stem cell CM (hASC-CM) with different basal medium on mice embryo development after parthenogenetic activation (PA). hASC-CM was collected from 2 kinds of serum free basal medium, DMEM and KSFM, respectively on day 5 from the culture of hASC isolated from human fat tissue. Intra-peritoneal injection of PMSG and hCG was conducted into 7-week-old ICR mice for superovulation. The oocytes were recovered from the oviductal ampulla, 18 h after hCG injection, and denuded using 0.1% hyaluronidase. PA of oocytes was conducted with KSOM media including strontium chloride. The parthenotes were in vitro cultured in 3 groups: 100% KSOM (Control), 75% KSOM + 25% DMEM or KSFM without FBS (DMEM or KSFM group) and 75% KSOM + 25% hASC-CM from DMEM or KSFM (DMEM-CM or KSFM-CM group). Cleavage rate was assessed after 2 days post IVC and blastocyst formation rate was evaluated after 6 days post IVC both using stereomicroscope. Total cell number of blastocysts was counted by Hoechst staining. 1way ANOVA from Graphpad prism 5 was used for statistical analysis and the values are presented as means ± standard error of mean. As a result, blastocyst formation rate of DMEM-CM group (16.09±3.32%, P<0.05) was significantly lower than control and DMEM group (34.43±2.89% and 34.49±5.34%, P<0.05) but cleavage rate and total cell number of blastocysts showed no significant difference among groups. In case of KSFM, there was no significant difference in cleavage rate, blastocyst formation rate and total cell number of blastocysts among the control, KSFM group and KSFM-CM group. The sort of basal medium used for the CM collection affected the development of parthenotes during in vitro culture differently. Therefore, further research should be conducted to find out the alternative basal medium of CM able to improve the embryo development. This research was supported by Nature Cell (#550-20170028), Cooperative Research Program of RDA (CCAR, #PJ013954022018), Research Institute for Veterinary Science and the BK21 plus program.

This study investigated the use of bovine serum albumin (BSA) as alternatives to fetal bovine serum (FBS) in in vitro maturation medium. The oocyte maturation, cumulus cell-oocyte gap junctional communication, and development of bovine embryos were determined by assessing their cell number, lipid content, mitochondrial activity, gene expression and cryo-tolerance. Oocytes were cultured in TCM-199 supplemented with 1 μg/ml estradiol-17ß, 10 μg/ml FSH, 10 ng/ml EGF, 0.6 mM cysteine, 0.2 mM sodium pyruvate and either 8% BSA (BSA group), 10% FBS (FBS group), or neither BSA nor FBS (TCM group), and followed by in vitro fertilization and the zygotes were cultured in SOF-BE1 medium. The differences in embryo development between experimental groups were analyzed by one-way ANOVA. We have shown that the percentages of embryos that underwent cleavage and formed a blastocyst were non significantly different among all experimental groups (37.4 ± 1.5% for FBS group vs. 31.1 ± 3.9% for BSA group and 34.5 ± 1.6% for TCM group, six replicates were performed). Furthermore, there was no significant difference between the percentage of MII oocyte between FBS (71.8 ± 1.9%) and BSA groups (69.3 ± 2.3%). However, culture of oocytes with FBS increased (P < 0.05) the cumulus cell expansion as well as expression of gape junction proteins, CX37 and CX43, at both transcriptional and translation levels. We also found that FBS significantly increased total cell number and decreased the apoptotic index in day-8 blastocyst comparing to BSA group. The beneficial effects of BSA on embryos were associated with significantly reduced intracellular lipid content and increased mitochondrial activity in both oocytes and blastocyst. Taken together, these data suggest that supplementation of maturation medium with BSA, as alternatives to FBS, can be used as defined medium that support consistently the development of IVP bovine embryos.