This experiment was conducted to investigate effect of brine mineral water (BMW) on growth performance and properties of blood in weaning pigs. Treatments allotted were 0% (general water), 2%, 3% and 5% BMW. In growth trial, a total of 36 weaning pig barrows [(Landrace×Yorkshire)×Duroc] weaned after 21 days with an average initial weight of 5.38±0.89 kg were used. Each treatment had 3 replications of 3 pigs per pen in a randomized complete block design. Weaning pigs were investigated for growth performance, complete blood corpuscle count and blood biochemical assay. In results, growth performance of 2% and 3% treatment groups were significantly higher than other groups (p<0.05). In addition, high density lipoprotein cholesterol was significantly (p<0.05) higher in 2% group than other treatment groups. On the other hand, mean corpuscular volume of supplement of BMW treatment groups were significantly (p<0.05) increased than control. Therefore, this study suggests that supplementation of BMW could improve growth performance and level of red blood cell in weaning pigs.

Effect of brine mineral water(BMW) on contact hypersensitivity reaction(CHR) was estimated using trimellitic anhydride (TMA) induced CHR in the mouse. BMW exhibited potent inhibitory activity on TMA induced CHR. BMW treatment suppressed the ear swelling, and attenuated hyper-activated lymph nodes stimulated by TMA challenge, thereby reduced their weight. The immunological index was analyzed after BMW administration in CHR. The level of serum IGE was significantly down regulated after BMW treatment. Furthermore, BMW showed inhibitory property of cytokine production. BMW treatment suppressed not only Th2 type cytokine, IL-4 but also pro-inflammatory cytokines such as TNF-α and IL-6. From the histological analysis, the inflammatory context appeared by atopic dermatitis lesion after challenging with TMA are diminished by BMW treatment. Our results suggest that BMW may attenuate the development of CHR, and can be available for functional ingredient.

The objective of this study was to evaluate the sperm liquid storage diluted with Brine Mineral Water (BMW) in miniature pig. Therefore we performed to find optimal concentration of BMW. The ejaculated semen from miniature pig was collected by gloved-hand method. The collected semen was diluted with dilution solution (Mulberry Ⅲ; modified-Modena B) which BMW was added. Concentration of BMW was 0, 2.5, 5, 7.5, 10 and 12.5% in dilution solution. The diluted semen was preserved at 17℃. Sperm ability was estimated by viability, capacitation acrosome reaction using chlortetracycline (CTC), hypoosmotic swelling test (HOST), morphologic abnormality. The diluted semen was observed for 7 days. The viability was significantly measured higher at 2.5% concentration of BMW than other groups (p<0.05). The AR-pattern of CTC analysis was significantly measured lower at 12.5% concentration of BMW than other groups (p<0.05). However, abnormality was not significantly different among all the groups (p<0.05). In conclusion, viability of sperm was the highest at 2.5% concentration of BMW but BMW had a negative effect on HOST, capacitation and acrosome reaction in sperm of miniature pig.

지하 943 m(3 호공)로부터 취수한 경산 광천수의 유·무기성분을 분석한 결과 총 17종류의 성분 중 무기물은 11종류가 검출 되었으며 무기성분 중에서는 나트륨과 칼슘 성분이 많이 함유되어 있는 것이 특징 이었다. 그리고 구리와 납과 같은 중금속과 탄산가스 및 유화수소 검출되지 않았다. 그리고 항산화 활성, 항돌연변이원성 및 인간 암세포 성장억제 실험 결과 항산화 활성에서는 비교적 약한 활성을 나타내었다. MNNG, 4NQO, Trp-P-1 및 B(α)P와 같은 돌연변이원을 이용한 미생물 수준에서의 항돌연변이원성 실험 결과 S. typehimurium TA98에 대해서 간접변이 원인 Trp-P-1은 시료 농도 200μg/plate 첨가 시 54%의 억제활성을 보였으며 B(α)P 및 Trp-P-1에 대해서도 각각 67%와 63%의 높은 억제활성을 나타내었다. 그리고 광천수의 항암활성을 규명하기 위한 암세포 성장억제 효과에서는 시료농도 50 μg/well 첨가 시 A549, Hela, AGS 및 MCF-7에 대해서 각각 66%, 45.6%, 37.7% 및 47.6%의 억제효과를 나타내었다. 또한 인간 정상 신세포 293에 대한 온천수 농도에 따른 세포독성 효과는 20% 이하는 낮은 생육 억제율을 보였으며 이것은 광천수가 정상세포에 대해서는 비교적 낮은 독성효과를 나타냄을 알 수 있었다.