본 연구는 헛개나무 용기묘를 생산하기 위한 적정 차광수준을 밝히고자 수행되었다. 헛개나무 1년생 용기묘를 대상으로 4가지 차광처리(0%, 35%, 55%, 75%)에 따른 용기묘의 간장, 근원경, 건중량, 묘목품질지수, 엽록소 함량 등의 반응 특성을 조사하였다. 연구 결과, 헛개나무 용기묘의 근원경, 잎 건중량, 뿌리 건중량은 차광처리구 간에 통계적인 유의성이 나타나지 않았으나(p≥0.05), 간장, 줄기 건중량, 엽록소 함량은 차광처리에 의해 55%와 75%의 차광처리에서 유의적인 차이를 나타내며 증가하였다(p<0.001). 간장과 줄기 건중량의 증가로 헛개나무 용기묘의 H/D율과 T/R율은 증가하였으며 묘목품질지수는 낮게 나타났다. 따라서, 헛개나무 1년생 용기묘 생산을 위한 적절한 차광수준은 묘목품질지수가 가장 높게 나타난 35%로 판단된다. 본 연구결과는 우량한 헛개나무 용기묘 생산을 위한 기초자료로 활용될 수 있을 것으로 기대된다.

본 연구는 피음처리에 따른 1년생 가시나무 용기묘의 생장과 생리적 반응을 탐구하고자 수행되었는데, 피음처리는 전광 및 전광의 35%, 55%, 75%의 수준으로 실시하였다. 가시나무의 간장과 근원경생장은 35%와 55% 피음처리에서 높은 것으로 조사되었으며, 피음처리와 상관없이 근원경생장이 간장생장 보다 4주 정도 더 길게 지속되었다. 피음처리 후 가장 높은 H/D율은 4.31을 기록한 35% 피음이었으며 가장 낮은 값은 3.63을 기록한 75% 피음이었다. 또한 피음처리후 가장 높은 건물생산량은 55% 피음, 그 다음으로는 35% 피음이었는데, 부위별로도 이 두 피음수준에서 가장 높은 것으로 조사되었다. T/R율은 75% 피음에서 1.76으로 가장 낮게 나타났고 55%에서 2.41로 가장 높게 나타났으며 35%에서는 2.38로 조사되었다. Leaf dry weight ratio(LWR)의 경우, 피음처리 후 가장 높은 값은 55% 피음의 0.53, 다음은 35%의 0.52이었으며, root dry weight ratio(RWR)은 피음수준이 가장 높은 75%에서 0.36으로 가장 높은 것으로 조사되었다. 피음이 강해질수록 엽록소 a 함량과 총 엽록소(a + b) 함량은 높아지는 경향을 보였지만 엽록소 b의 경우에는 피음처리에 따른 차이가 나타나지 않았다. 그리고 광합성률과 증산율은 전광에 비해 피음이 높을수록 증가하였으나, 가장 높은 피음인 75%보다는 35% 및 55% 피음에서 더 높았다. 본 실험에서 피음처리에 따른 광 조건이 다른 환경에서 생육한 묘목들이 보인 생장과 광합성 반응 결과는 적정 생육광도를 설정하는데 유용하게 사용될 수 있으며, 또한 내음성 정도를 추정하는 자료로도 활용될 수 있을 것으로 사료된다.

본 연구에서는 중국 북경 옌칭(延慶)으로부터 북한 상원지역에 도입한 1년생 묘목 4배체 아까시나무를 대상으로 식재 입지별로 1년생 묘목의 생장특성을 분석하고, 엽내 3가지 성분의 함량 측정을 통해 향후 식재 후 수확한 엽의 사료첨가제로 이용 가능성을 검토하였다. 그 결과, (1) 상원지역에서 평지에 식재한 4배체 아까시나무는 중국의 옌칭지역 내 4배체 아까시나무에 비해 당년생 맹아지의 길이와 근원경이 50%정도 작게 나타났다. (2) 상원지역의 4개 사면입지에서 4배체 아까시나무의 당년생 맹아지의 길이 생장은 동, 서, 남사면에서 북사면에 비해 23~30% 크게 나타났고, 근원경은 동, 남사면의 4배 아까시나무가 북, 서사면에 비해 14~23% 크게 나타났다. (3) 상원지역에서 4배체 아까시나무의 엽내 조단백질과 조회분 함량은 중국 옌칭지역 내 4배체 아까시나무와 마찬가지로 일반 아까시나무 잎에 비해 40% 이상 높게 나타났다.

사과 ‘후지’/M.9 포트묘목 개발을 위해 질소시비농도에 따른 생장특성을 조사하고, 포트 우량묘목 생산에 적합한 질소시비 농도와 잎의 무기영양성분 함량 및 토양 화학성의 안정성을 확인하였다. 질소시비농도가 높아질수록 묘목의 생장은 증가되 었고, 특히 16 mM 처리가 수체 생장에 가장 좋았으며, 우량묘목 판단기준에 부합하였다. 32 mM 이상의 고농도는 오히려 생장 을 감소시켰다. 잎의 무기영양성분 함량은 8, 16 mM 처리구에서 기존 사과과원의 적정수준보다 높았고, 이러한 무기영양성분은 정식 후 수체 생장에 도움이 될 것으로 생각되었다. 토양 화학성 또한 8, 16 mM 처리구에서 안정적이었다. 따라서 수체 생육, 잎의 무기영양성분, 포트 내 토양화학성을 고려한 결과, 사과 ‘후지’/M.9 우량 포트묘목 생산을 위한 적정 질소시비량은 16 mM로 판단되었다.

Background: Ginseng produced by hydroponics can be cultivated without using agricultural chemicals; thus, it can be used as a raw materials for functional foods, medicines, and cosmetics. This study aimed to determine the optimal harvesting time to obtain the highest levels of ginsenoside and ginseng, as this was not previously unknown. Methods and Results: One-year-old organic ginseng seedlings were transplanted and cultivated using hydroponics for 150 days in a venlo-type greenhouse, using ginseng nursery bed soil and a nutrient solution (NO3 −-N; 6.165, P; 3.525, K; 5.625, Ca; 4.365, Mg; 5.085, S; 5.31 mEq/ℓ). Ginsenoside content and fresh and dry weights were higher at 120 days after transplanting than at 30, 60, 90, and 150 days. Total ginsenoside content was 11.86 times higher in the leaf and stem than in the root at 120 days after transplanting. Ginsenosides F1, F2, F3, and F5 were detected in ginseng leaves and stems. These chemical compounds are known to be effective in altering skin properties, including whitening, anti-inflammation, and anti-aging. Conclusions: Optimal harvesting time for ginseng cultivated using hydroponics was 120 days after transplanting when the biomass and ginsenoside content were highest.

Background : Panax ginseng C. A. Meyer is an important medicinal herb and their major pharmacologically active components are ginsenosides. Many studies have reported that various ginsenosides are effective in the treatment of human diseases such as cancer, cardiovascular and alzheimer’s disease. However, it is difficult to create new desirable variation such as increased ginsenosides. Since the frequency of spontaneous mutations is rare naturally and difficult to apply to plant breeding, artificial mutation inductions are necessary to obtain noble genetic resources having desirable traits. Methods and Results : Gamma irradiation was carried out using a 60Co irradiator (3000Ci, Nordion Inc., Ottawa, Canada) of the Korea Atomic Energy Research Institute, Jeongeup, Korea. One-year-old ginsengs were treated at 20, 40, 60, 80, and 100 Gy. The emergence rate was significantly decreased with increasing gamma doses. When irradiated at more than 80 Gy, it did not emerge at all. Survival rate was also significantly influenced by gamma-ray dose. The maximum survival rate were recorded in control. The survival rate of 20 Gy was 22.0% and only 1.0% survived when 40 Gy dose was treated. There was no survival when irradiated at more than 60 Gy. Gamma irradiation also affected the phenotype of emerged plants. The growth of plants derived from irradiated one-year-old ginsengs was decreased and the leaves became variegated or curled. Also, rusty roots resulting from physiological disorders were observed and the rhizomes, which is a kind of dormant bud, were destroyed completely. Conclusion : Based on these results, we estimated the optimal dose for mutation induction by gamma-ray treatment of one-year-old ginseng is to be less than 20 Gy.

Background : The insufficient soil moisture due to spring drought causes the germination rate of ginseng seeds to be remarkably lowered and the low seeding depth causes the roots not to be settled but remain in the surface soil containing moisture, resulting in the ill-shaped ginseng. Especially, in the case of paddy soil, the soil environment is often over-humidified or over-dried, resulting in high rate of occurrence of physiological disorders such as rough skin, yellow-colored root and red-colored root, etc. compared to the upland soil, requiring more care in managing the soil. Therefore, this study was conducted with the aim to investigate the effects of stamping treatment on the direct seeding cultivation in the paddy soil and to investigate the survival and growth characteristics according to the seeding method. Methods and Results : We investigated the effects of stamping on the emergency rate and growth by using rollers (15, 20, 25, 30 ㎏), and also investigated the growth characteristics according to the direct sowing methods (broadcast planting, motorized seeder, semi-automatic seeder, manual seeder, hoop-type seeder). In the emergency rate of 1-year-old ginseng group directly sowing and treated with trampling, the group treated with 25 ㎏ & 30 ㎏ showed the highest emergency rate & growth, and above-ground & underground parts growth of 1-year-old ginseng by sowing method, there was no significant difference between sowing methods, the emergency rate of motorized seeder and semi-automatic seeder was high. Conclusion : In the paddy soils, the 25 – 30 ㎏ stamping after direct seeding showed superior above-ground growth and emergency rate, and there was no significant difference in growth, but motorized seeder and semi-automatic seeder had a higher rate of emergency in 1-year-old ginseng.

Background : This study was conducted to investigate the seedling, growth, and yield characteristics of 1-year-old Polygala tenuifolia by cultivation of different regions. Methods and Results : After growing the seedlings for 2 months in the greenhouse, they were planted at different altitudes including: 100 m or less (Cheongju), 250 m (negative), 300 m (Jecheon), and 500 m (Pyeongchang) in the first week of May. The flowering period, fertilization period, fruit weight, growth, and yield were recorded and investigated in that period. Results of analyzing the physicochemical properties of the soils revealed that the pH of the soil was close to neutrality of 6.7 - 7.3 at 100 m, 250 m, and 500 m altitudes and an acidity of pH 5.7 at 300 m altitude. The average temperature for different time periods and altitudes are the following: 3.8℃ from June to October during the harvesting season, 21.8℃ at 100 m altitude in the end of June, 10.8℃ at 500 m altitude in the middle of harvest period, and 2.7℃ higher at 100 m altitude than 500 m altitude. The difference in the number of rooting (between 11 and 12/㎡) and the rate of rooting (82 - 92%) was not significant. The plant length was 25 - 26.5 ㎝ at 300 m and 500 m altitude. The largest number of branch was at 1.8 with 250 m altitude and largest stalk diameter at 500 m altitude at 6.0 ㎜. Leaf length and leaf width were the same per area, but the branches were the largest at 500 m at 17.9. Dry weight of 12.7 g was heaviest at 500 m altitude implying that the higher the altitude, the higher the dry weight will be. Moreover, in the 500 m altitude, root length of 29.2 ㎝ was the longest, root diameter was 5.9 ㎜, and the dry root weight of 7.9 g was the heaviest. However, the number of supporting root was 4.9 - 7.3 which was not statistically significant. The optimal yield per 10 a was 94 ㎏ at 500 m altitude. This was very high compared to yield of 28 ㎏ at 100 m altitude. The seed weight per 10 a was 1.5 ㎏ and 2.3 ㎏ at 100 m and 300 m altitude, respectively. The highest content of Teuifolin contained in the root was 124.07 ppm at 500 m altitude. Conclusion : Results showed that 1-year-old P. tenuifolia had optimal growth, higher yield and better vital component content at altitude of 500 m.