The soybean aphid, Aphis glycines Matsumura 1917, is well known as a soybean pest in the world. Recently, it has been introduced to North America causing serious damage in U.S. As a cooperative research with USDA-ARS, we have investigated A. glycines in soybean fields, and also examined the colonies on the overwintering host Rhamnus davurica in order to find its natural enemies. It was generally reported that A. glycines has host alternation between the soybean, Glycine max (summer host) and the Dahurian buckthorn, Rhamnus davurica (winter host) in East Asia. However, it was very difficult to identify the soybean aphid, A. glycines, from R. davurica due to the co-existance of at least three Aphis species and the seasonal polymorphisms of each species (e.g, gynopara, ovipara, and male). For species identification, we tested 3 molecular markers, mitochondrial COI, COII, and nuclear EF1α, for 14 collected samples (7 samples from G. max and 7 samples from R. davurica). As a result, we found two different species, A. gossypii and other Aphis sp., are mixed together with A. glycines on R. davurica. We report the biology of A. glycines in Korea, and present species identification using molecular phylogenetic approach.

Adult longevity and fecundity of the foxglove aphid, Aulacorthum solani Kaltenbach, were studied at 12.5~25℃ with 60~70% RH under 16L:8D and the results were put together to build a life table. The longevity of foxglove gradually increased with decreasing temperature below 25℃. Also total fecundity increased with decreasing temperature and highest fecundity was 74.1 nymphs per female at 15℃. However, daily fecundity increased with increasing temperatures up to 20℃ showing 2.9 nymphs per day and thereafter decreased. Net reproduction rate (R₀) was highest of 58.7 at 15℃. The intrinsic rate of increase per day (r<SUB>m</SUB>) and the finite rate of increase per day (λ) were highest of 0.27 and 1.32, respectively and the doubling time (Dt) was shortest of 2.52. the mean generation time (T) was 10.99 at 22.5℃.

The development of Aulacorthum solani (Kaltenbach) was studied at temperatures ranging from 12.5 to 27.5℃ under 65±5% RH, and a photoperiod of 16:8 (L:D). Mortality of 1st~2nd nymph was higher than that of 3rd~4th nymph at the most temperature ranges whereas at high temperature of 27.5℃, more 3~4th nymph stage individuals died. The total developmental time ranged from 16.9 days at 12.5℃ to 6.6days at 22.5℃, suggesting that higher the temperature, faster the development. However, at higher temperature of 25℃ the development took 7.4 days. The lower developmental threshold temperature and effective accumulative temperatures for the total immature stage were 0.08℃ and 162.8 day-degreeslated development. The nonlinear shape of temperature rewas well described by the modified Sharpe and DeMichele model. When the normalized cumulative frequency distributions of developmental times for each life stage were fitted to the three-parameter Weibull function, attendance of shortened developmental times was apparent with in 1~2nd nymph, 3~4th nymph, and total nymph stages in descending order. The coefficient of determination r² ranged between 0.86 and 0.91.

Fifty potato cultivars were screened for potato aphid, Macrosiphum euphorbiae Thomas infestation degree in the field. Aphid honey dew secretion and contents of foliar substances (glycoalkaloids, total nitrogen, Ca, K and Mg) of the cultivars were measured in the laboratory to analyze the correlation between infestation degrees and those factors. The number of aphids ranged from 4.1 to 31.4 per five leaves. The lower density (less than 5 aphids) was found on cvs. Jidose, Recent, and Sebago. The aphid secreted highest (40.3 droplets) and lowest (15.7 droplets) honeydew on cvs. Jopoong and Anco, respectively. Degree of infestation is positively correlated to glycoalkaloids and total nitrogen contents of the potato leaf.

The turnip aphid is a worldwide pest, damaging mainly to crucifers. In order to understand the life parameters of Lipaphis erysimi for the eventual goal of control, the developmental periods, survival rates, lifespan, and fecundity of the species were investigated under five temperature regimes (15℃ - 35℃). Furthermore, the efficacy of several environment-friendly agricultural materials (EFAMs) that are on the market was subjected to test in order to obtain further accurate information. The developmental period of the turnip aphid nymph was longest at 15ºC as 16.9 days, shortened as temperature goes up to 25ºC (5.4 days), and then somewhat increased at 30ºC (5.9 days), suggesting that the most efficient temperature for nymphal development could be around 25ºC. Mortality of the nymphal turnip aphid was obvious at 35ºC, whereas it was minimal at other temperature schemes. The longevity of adults shortened as temperature goes up to 30ºC. In particular, the maximum lifespan for adults continued for 55 days at 15ºC, but shortened to 21 days at 30ºC. The total fecundity per day was 35.7 at 15ºC, 81 at 20ºC, 64.2 at 25ºC, and 6.6 individuals at 30ºC, showing the highest fecundity at 20ºC. After the turnip aphids were successfully stabilized in indoor environment the insecticidal activity was tested and mortality was determined 12, 24, 36, and 48 hrs after EFAMs are treated. Several on-the-market EFAMs showed more than 90% of insecticidal activity within 24 hrs or 48 hrs, but a few showed less than 90% activity, signifying importance of selection of proper EFAMs.

As the results achieved by the evaluation of toxicities on an aphid parasitoid, Aphidius colemani by 79 pesticides registered as horticultural pesticide and 4 adjuvants for pest control, 6 insecticides including a-cypermethrin, 13 fungicides including metalaxyl-M+mancozeb and 4 acaricides including bifenazate showed low toxicity against A. colemani adult. Low toxicity was showed in all the 4 adjuvants as well. In residual toxicity test from 40 pesticides which showed toxicity more than 50%, A. colemani was safe from 11 pesticides from the 3th day after treatment, 7 pesticides from the 5th day after treatment and 14 pesticides from the 7th day after treatment, respectively. But, chlorpyrifos-methyl, diflubenzuron+chlorpyrifos, etofenprox+diazinone and imidachloprid+chlorpyrifos showed high toxicities reaching 100%, 97.7%, 100% and 100% respectively, even from the 7th day after treatment. To evaluate the control effect by A. colemani against Myzus persicae in a greenhouse, A. colemani was released at parasitoid versus aphids rates of 1:50 and 1:100 when the population of M. persicae was 50 per plant. After release, aphids population remained steady for 20 days after release at the level of around 60 aphids per plant During the investigation, insecticides for thrips control and fungicides for powdery mildew control were treated, but didn"t affect the mummy forming of A. colemani. It may be suggested from these results that the selected insecticides, fungicides, acaricides and adjuvent could be incorporated into the integrated M. persicae management system with A. colemani on greenhouse cultivation.

복숭아혹진딧물의 섭식행동을 관찰하기 위해, 고추, 무, 배추, 가지, 참외 등 5종류의 기주식물에서 EPG기술을 이용하였다. 복숭아혹진딧물의 구침이 식물체 표면에 접촉된 후 전기적 연결이 일어나기까지 소요되는 시간과 맨 처음 potential drop이 일어나기까지 소요된 시간, 구침의 세포간극내에서의 활동에 의하여 일어나는 potential drop의 수, 전기적 연결신호가 나타난 시점부터 체관부를 섭식하는 순간까지 소요된 시간 등을 조사한 결과 Potential drop 수에서는 기주들 사이에 유의성 있는 차이를 나타내었다. 그러나 나머지 요인에서는 기주식물들 사이에 큰 차이를 보이지 않았지만, 참외나 배추보다는 고추, 무, 가지에서 즘 더 기주선호성과 관련된 섭식패턴들을 확인할 수 있었다.

복숭아혹진딧물(Myzus persicae )의 성충 발육과 산자수를 , 상대습도 60-70%, 광주기 16L:8D 조건에서 조사하고 생명표를 작성하였다. 복숭아혹진딧물 성충은 까지 수명이 일로 온도의 상승에 따라 성충기간이 짧아졌으며, 산자수는 에서 40.8마리로 최고치를 보였으나 까지 산자수 차이에 유의성이 인정되지 않았다. 다만 에서는 8.4마리로 고온에 영향을 받은 것으로 보인다. 또한 복숭아혹진딧물 성충의 일일 산자수는 에서 5.1마리였고, 와 에서는 모두 2.8마리로 온도가 높거나 낮아지면서 일일산자수가 적어졌다. 온도별 총 산자수를 이용하여 작성한 산자 모형을 보면 에서 51.0마리로 최고값을 보인다. 또한 약충 발육시 사망률과 성충 수명, 산자수를 이용하여 생명표를 작성하였는데 복숭아혹진딧물은 Ro가 에서 36.5, 은 0.4, Dt는 1.6, 기간증가율은 1.5, 세대기간은 8.1로 가 복숭아혹진딧물 증가에 가장 적합한 온도로 나타났다.