Ainsliaea acerifolia leaves are registered with the Ministry of Food and Drug Safety as edible herbal materials in Korea, and research is underway to explore their potential in developing functional foods, cosmetics, and pharmaceuticals. In this study, we developed an analytical method using HPLC-DAD to quantify three key compounds—chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid—in A. acerifolia leaves extract. This method has been optimized and validated for specificity, accuracy, precision, limit of quantification (LOQ), and linearity. The correlation coefficients (r²) for the calibration curves exceeded 0.9962. The limits of detection (LOD) and quantification (LOQ) were 0.3012 and 0.9128 μg/mL for chlorogenic acid, 0.1182 and 0.3582 μg/mL for isochlorogenic acid A, and 0.2342 and 0.7098 μg/mL for 1,5-dicaffeoylquinic acid, respectively. The net recovery rates for accuracy testing were 105.13% for chlorogenic acid, 105.37% for isochlorogenic acid A, and 100.37% for 1,5-dicaffeoylquinic acid. All parameters assessed with this newly developed method fell within the acceptable ranges specified by ICH guidelines. These findings demonstrate that the method is robust and reliable for accurately identifying and quantifying chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid in both routine analysis and large-scale extraction process of A. acerifolia leaves.

The objective of this study was to develop a microcapsule by the entrapment of Ainsliaeaacerifolia extract (AAE) in native starch and citrate starch matrices through spray-drying and dry heating reaction. AAE microcapsules were assessed based on recovery, encapsulation capacity, FT-IR, FE-SEM, swelling power, solubility, releasing pattern of AAE from microcapsules, and antioxidant activity. Upon increasing the addition levels of AAE and adding citric acid in the pre-emulsion mixtures, the spray-drying recovery and encapsulation capacity of AAE microcapsules increased. AAE microcapsules exhibited irregular shapes due to excessive shrinkage of their spheres. FT-IR spectra implied the formation of ester groups between starch molecules and either citric acid or phenolic compounds in AAE. Swelling powers and solubility of AAE microcapsules were significantly higher for citrate starch matrix (relative to native starch). The effects of temperature and enzyme hydrolysis on AAE releasing patterns were similar over AAE microcapsules prepared in this study. AAE microcapsules facilitated the free radical scavenging in an aqueous (relative to alcoholic) reaction system. Overall, the entrapment of AAE into the matrices from native starch and citrate starch would be one of the possible ways to expand the utilization of raw materials, by-product, and extract from Ainsliaeaacerifolia in food industries.

국화과에 속하는 단풍취의 추출물이 가지는 알츠하이머에 대한 효과를 알기 위하여 AChE에 대한 억제효과와 peroxy-nitrite 소거효과를 연구하였다. 단풍취 추출물은 AChE에 대한 억제효과로서 그 IC50가 73.4 μg/ml이었으며 peroxynitrite 소거효과에 관한 IC50는 8.60 μg/mL이었으므로 알츠하이머 질환에 유익할 것으로 판단된다. 단풍취에 가장 많이 함유된 성분인 3,5-dicaffeoylquinic acid 성분의 활성도 이와 유사한 활성을 보였으므로 단풍취 추출물의 효과는 주로 이 화합물의 활성에 따른 것으로 예상된다. Caffeoylquinic acid가 알츠하이머 질환에 효과있다는 보고가 있으므로 이 식물의 잎과 줄기 추출물에서 6종의 지표물질을 이용하여 정량법을 확립한 후 정량하였다. 그 결과 잎에서는 성분 함량이 25.1%를 차지하여 줄기의 함량보다 훨씬 높았다. 특히 잎 추출물 중 3,5-dicaffeoylquinic acid의 함량이 145.6 ㎎/g에 달하여 알츠하이머 질환 치료에 유익할 것으로 예상된다.

단풍취를 70% 에탄올로 침지 추출하여 얻어진 추출물에 대해 n-hexane, EtOAc 및n-BuOH로 순차 용매 분획하였고,얻어진 결과물에 대하여 DPPH와 ABTS+ radical 소거능및 α-glucosidase 저해활성을 평가하였다. DPPH 라디칼 소거능은 페놀성 화합물의 함량이 상대적으로 높은 EtOAc층에서 IC50 값이 23.4±0.3 mg/mL으로 강한 DPPH 라디칼 소거능을 확인하였고, 단풍취 추출물에 존재하는 페놀성 화합물과 라디칼 소거능과의 연관성을 시사하였다. 또한 ABTS+ 라디칼 소거능은 EtOAc층의 IC50 값이 19.9±2.3mg/mL, n-BuOH층이 IC50 값이 23.4±0.3 mg/mL의 우수한라디칼 소거활성이 확인 되었고, 강한 활성물질의 존재가 시사되었다. 또한, α-glucosidase 저해활성을 측정한 결과, 강한 ABTS+ 라디칼 소거능을 나타낸 EtOAc 층의 IC50은 103.4±1.0 mg/mL의 저해활성을 나타내었으며 이는 positive control인 acarbose에 비해 우수한 활성이었으며, 추출물 상태의 시료를 단일물질로 정제할 경우 더욱 강한 효능의 화합물이 존재할 가능성을 시사하였다. 향후 이들 활성물질 동정을 통한 활성 기작에 대한 연구가 필요하며 본 연구결과는 보다 우수한 라디칼 소거능 및 α-glucosidase 저해능을 가지는 새로운 기능성 식품소재 발굴을 위한 기초자료로활용가능하리라 사료된다.

This study was carried out to investigate the morphological characteristics of the plants and seeds of Ainsliaea acerifolia and to determine the optimum condition for producing sprouts from the seeds. Plant height, flower stalk length, and pod number were higher in natural habitat than in campus farm. Average 1.2 seeds per pod was set but only 0 to 2 seeds per plant was set in plants with the enveloped flower stalks, indicating that this is an outcrossing species. Most of seeds were 9-11mm long and 1.1-1.4mm wide. Fresh weight of seeds was ranged from 10mg to 17mg. Seeds germinated well at 15℃ and 20℃. Mean germination period was 11.5 day at 15 to 25℃. Sprouts grown at 15℃ was longest(5.4cm) and heaviest(738mg/10 sprouts). Chlorophyll content was 333mg per fresh weight 110g. Protein, Fe, vitamin Bl, vitamin B2, and vitamin C were respectively 23.7mg, 6.4mg, 1.82mg, 0.49mg, and 10..7mg.