한반도의 Donacia속에는 2아속에 속하는 6종이 있다(An, 2019). 이 중 Chujo (1941)는 북한 함경북도 웅기에서 채집한 표본을 D. aquatica (Linnaeus, 1758)로 보고하였고, Kimoto and Kawase (1966)는 D. japana로 표기하여 보고하였다. 그리고 Hayashi (2020)는 한국에 기록된 D. japana가 D. aquatica로 오인되었을 수 있다고 밝혔다. 이러한 혼란을 해결하기 위해 본 연구는 한국(포천과 울산)에서 채집한 표본과 D. japana에 대한 기재문, 수컷생식기와 같은 형태적인 부분(Hayashi, 2020), COI 유전자 염기서열을 비교하였다. 그 결과로 한반도에 기록된 D. japana은 D. aquatica로 대체되고, D. japana는 일본 고유종이라고 결론을 내리고자 한다.

쇠고기 이력추적제는 소 개체마다 이력번호를 부여하여 수입쇠고기가 한우고기로 둔갑하는 사례를 막고, 소비자 에게 구입한 한우고기의 세부 이력 정보들을 제공하는 데 도움을 준다. 이번 연구는 2021년-2022년 서울지역 대형 축산물판매업소에서 유통되는 한우고기 344건에서 DNA 동일성 검사를 실시하여 이력번호 등 주요 표시정보들의 진위를 판별하였다. 그 결과 45건(13.1%)에서 이력번호가 불일치한 것으로 확인되었다. 불일치율은 2021년(14.7%) 에 비해 2022년(11.3%) 감소하였고, 도심서북권 20.7%, 동 북권 14.4%, 서남권 및 동남권 10.2% 순으로, 북부권 (16.9%)이 남부권(10.2%)에 비해 높게 나타났다. 또한 6개 브랜드 중 B사 및 D사는 이력관리가 양호한 반면, E사 및 A사는 이력관리가 취약한 것으로 확인되었고, 통계적 으로 유의한 결과 차이를 보였다(P<0.001). 이력번호 불일 치 시료의 실제 이력번호는 업소 내 입(출)고 거래내역 자 료를 근거로 조사하였고, 그 결과 실제 이력번호 확인율 은 53.9%에 불과하였으나, 확인된 범위 내에서 시료의 표 시정보 진위 판별 결과, 거짓 표시는 이력번호 13.1%, 성 별 2.9%, 도축장명 2.2%, 등급 1.6% 순으로 나타났고, 품종 (한우) 거짓 표시는 없었다. 거짓 표시 축산물의 유통 차 단을 위해 표시정보의 신속한 진위 판별이 중요하므로 업 소 내 이력번호가 기재된 날짜별 부분육 소분할 작업내역 작성을 의무화하는 법적 근거 마련이 필요하다. 본 연구 결과는 투명한 축산물 유통 거래 질서 정착을 위한 이력 관리 방향 설정에 참고자료로 활용하고자 한다.

Enforcement of the beef traceability system can block the sale of imported beef disguised Hanwoo and help customers to know detailed information of the Hanwoo beef while purchase. This study was carried out to investigate the possibilities of identifying DNA upon various ripening and roasting methods (soy sauce, gochujang sauce, 10% vinegar, 10% coke, 10% Cheongha, 10% Soju, completely burned out, raw, normal roasted, dried, and boiled) employed for beef traceability system. Completely burned out meat could not yied useful DNA in the sample, hence wa not analysed. The amplified peaks in most of the samples appeared equally barring completely burned out meat. However, roasted beef after ripened in soy sauce showed new peaks or deletion of a peaks with two markers. The DNA allele height and the amount of extracted DNA in roasted beef after ripening in 10% Cheongha was found to be higher than those in the raw beef. No significant correlations between DNA allele height and the amount of extracted DNA within the samples was noticed. Also, these results suggest that beef traceability using microsatellite (MS) markers is possible in various roasting beef because of bovine specific MS markers. Therefore, beef traceability system by DNA identify test would give the confidence in food safety and beef distribution system to customers.

Analysis of meat quality grade based on the DNA identity test samples were done on 320 Hanwoo (Korean cattle) raised in Gyeonggi region of Korea. 62.29% of cows and 91.35% of steers showed high quality which was above 1st beef grade. However, differences were observed between Gyeonggi Hanwoo and total slaughtered Hanwoo in Gyeonggi in the year 2009, though the sampling was entirely based high quality meat. Meat samples of pedigree registered cattle showed high rate of above 1st meat grade compared to advanced and fundamentally registered cattle. Pedigree registry is associated with quality grade of beef like the sex of cattle. Appearance rate of above 1st beef grade in offsprings of the top 9 Korean Proven Bulls produced through mating to Gyeonggi Hanwoo was 43.27% which was found to be higher than the average grade in cows (47.3%) and was 13.08% higher than the average grade in steers (77.49%). Our results showed superior genetic characters of Korean Proven Bulls which favors and influence the meat quality grade of their offsprings. Furthermore, our results claim that sex of Hanwoo irrespective of pedigreed or Korean Proven Bulls affects and/or influence the meat quality. Conclusively, the results could therefore be used as a basic data for the improvement of beef quality. However, paternity test by DNA typing and huge sample needs to be analysed to authenticate the findings.

Beef traceability, a system that provides all the records of beef production, helps customers purchase that they get to know detailed information of the Hanwoo beef. This study was carried out to investigate the DNA identity possibility in various cooking methods to ripen meat (soy sauce, gochujang sauce, 10% vinegar, 10% coke, 10% Cheongha, 10% Soju, raw, dried, and decayed) for beef traceability. The DNA content of decayed beef was higher than those of other cooking methods. It is thought that result of mixed pollutant, it did not affect amplified DNA allele height because of bovine specific microsatellite (MS) markers. The ripened sample in 10% vinegar 3 days was lowest a mount of extracted DNA (156 ng) and amplified DNA allele height (based on the raw samples to 38%) by MS markers compared with the other cooking methods. There are no significant correlation between amplified DNA allele height and the amount of extracted DNA. Therefore, beef DNA identity test in various cooking methods to ripen meat can used by bovine specific MS markers. Beef traceability system by DNA identify test will give more confidence in food safety to customers

In this study, the analysis of meat quality grade was carried out on the DNA identity test samples of 452 Hanwoo (Korean cattle) raised in Gyeongnam region in the year 2009. Appearance rate of above 1st grade from cow (70%) and steer (93.16%) showed high quality grade. On the other hand, there were the difference between this study and slaughtered Hanwoo in Gyeongnam in the year 2009 - appearance rate of above 1st grade from cow (58.77%) and steer (82.99%). It expect considered influence of the samples for beef DNA identity test that collected mainly high quality meat. Quality grade by registry classification about collected samples showed correlation between pedigree classification and quality grade that appearance rate of above 1st grade highly observed pedigree registry more than advanced registry, advanced registry than fundamental registry. Although limited to DNA identity test samples, KPN (Korean Proven Bull's Number) 452 and KPN 407 was preferred for breeding in Gyeongnam. Also average appearance rate of above 1st grade in offsprings of the top 10 Korean Proven Bulls used mating is 95.35%. It means that Korean Proven Bulls influence meat quality of offsprings positively. Thus, this analysis is expected to be a base data for the improvement of meat quality of Hanwoo raised in Gyeongnam region.