This study was aimed at assessing the fumigant toxicity to adult Thrips palmi and Orius strigicollis of basil (Ocimum basilicum) essential oil compounds and structurally related compounds using vapour-phase toxicity bioassays. Results were compared with those for dichlorvos. Against adult T. palmi, linalool (LD50, 0.0055 mg cm–3) was the most toxic fumigant and was 15.2–fold more effective than dichlorvos (0.0837 mg cm–3). Strong fumigant toxicity was also observed in pulegone (0.0095 mg cm–3), (±)-camphor (0.0097 mg cm–3) and 1,8-cineole (0.0167 mg cm–3). Moderate toxicity was produced by camphene, 3-carene, (–)-menthone, (+)-α-pinene, (+)-β-pinene, α-terpineol and (–)-α-thujone (0.0215–0.0388 mg cm–3). Against adult O. strigicollis, dichlorvos (LD50, 9.0 ×10–10 mg cm–3) was the most toxic fumigant, whereas the LD50 values of these compounds ranged from 0.0127 to >0.23 mg cm–3. Based upon selective toxicity ratio (STR, O. strigicollis LD50/T. palmi LD50), the compounds described (STR, 0.7–>10.7) are more selective than dichlorvos (STR, 10.8×10–9 mg cm–3). Basil oil compounds described merit further study as potential insecticides for control of T. palmi in greenhouses because of their generally lower toxicity to O. strigicollis and their greater activity as a fumigant than dichlorvos.

With the comparison of native Orius strigicollis (Poppius) to European O. laevigatus (Fieber), we investigated biological characteristics such as developmental period, fecundity, life span, predation ability, and augmentation after release. Experiments were carried out at five temperature condition, 15℃, 20℃, 25℃, 30℃, and 35℃. In the case of O. strigicollis, egg period was 3.1~14.9 days (hatchability: 46.7~88.2%), and developmental period of nymphs was 9.4~42.8 days (survival rate: 2.4~96.5%). Fecundity at each temperature was 28.1, 107.9, 123.6, 127.3, and 18.1, respectively. Life span of adults was 63.4, 40.1, 22.1, 18.3, and 11.0 days, respectively. In the case of O. laevigatus, egg period was 3.1~13.5 days (hatchability: 65.0~89.9%), and developmental period of nymphs was 9.3~42.0 (survival rate: 4.3~80.6%) days. Fecundity at each temperature was 101.8, 218.6, 224.5, 219.5, and 15.7. Life span of adults was 70.6, 66.4, 32.6, 34.5, and 7.3 days, respectively. In long-day condition (16L:8D), fecundity of O. strigicollis and O. laevigatus was 105.8 (18℃)~142.4 (25℃) and 109.5 (18℃)~191.5 (25℃), respectively. In short-day condition (10L:14D), fecundity of them was 1.0 (18℃)~31.8 (25℃) and 63.0 (18℃)~198.8 (25℃), respectively. Daily prey consumption of second instar Frankliniella occidentalis was 14.3 and 10.9, respectively. In greenhouse, density of O. strigicollis begins to increase from mid May and peaked about early July (1.8 individual/flower), while that of O. laevigatus begins to increase from mid March and peaked about early June (6.6 individual/flower).

For the development of integrated pest management system by harmonizing biological and chemical control, some experiments were carried out to select low toxic pesticides and to evaluate residual toxicity to natural enemies. Leaf dipping method and body dipping method were set up for evaluating toxicity to minute pirate bug, Orius strigicollis adult. We had tested 52 kinds of pesticides (33 insecticides, 19 fungicides) commonly used to control greenhouse insects, mites, and disease pests to natural enemies at the recommended concentration. Fourteen insecticides by body dipping method, 12 insecticides by leaf dipping method and 19 fungicides were selected as low toxic pesticides to O. strigicollis adult. After insecticide spraying at recommending dose on the sweet pepper plant, we examined residual effect of insecticides by introducing natural enemies on different days. Safety interval for introduction of O. strigicollis adult was established according to residual toxicity of pesticides. Safety insecticides at one day after treatment were pyraclofos, methomyl, thiodicarb, esfenvalerate bifenthrin, alpha-cypermethrin, etofenprox, fenvalerate, imidacloprid, acetamiprid, abamectin, emamectin benzoate, spinosad, indoxacarb. However, residual toxicity of neo-nicotinoids last up to 21 days to O. strigicollis adults.

Effectiveness of Orius strigicollis (Poppius) and Amblyseius cucumeris (Oudemans) as natural enemies against thrips were evaluated on greenhouse green pepper, sweet pepper and cucumber respectively. Control efficacy was calculated by the formula, (D<SUB>control</SUB> - D<SUB>treatment</SUB>)/D<SUB>control</SUB> × 100, where D<SUB>control</SUB> is the average density of thrips on the plots in which any natural enemy was not released and D<SUB>treatment</SUB> is the average density of thrips on the plots in which natural enemies were released respectively. As a result, control efficacies of O. strigicollis against Frankliniella occidentalis (Pergande) on green pepper and sweet pepper were 14.3~99.5% and 21.6~98.3%, respectively. In addition, control efficacy of it against Thrips palmi Kany on cucumber was 61.2-74.4%. Control efficacies of A. cucumeris against F. occidentalis on green pepper and sweet pepper were 12.9~38.3% and 17.1~87.0%, respectively. Control efficacy of it against T. palmi on cucumber was 90.4~97.4%. Field evaluation showed that the prompt applications of natural enemies were effective to reduce the density of thrips. In detail, to control F. occidentalis effectively on green pepper and sweet pepper in spring season, five to six individuals of O. strigicollis per crops should be released three to six times continuously. To control T. palmi effectively on cucumber in autumn, more than 100 individuals of A. cucumeris per crop should be released four times repeatedly.

병해충 방제약제로 등록된 51종의 농약(살충제 25종, 살비제 11종, 살균제 11종 그리고 농약보조제 4종)에 대한 으뜸애꽃노린재 약충과 성충의 독성을 조사하였다. 살충제 중에서는 flpronil, lufenuron, acetamiprid+fipronil, -cypermethrin+flufenoxuron, buprofezin+amitraz of 독성이 낮았고, 살비제 중에서는 acequinocyl, bifenazate, chlorfenapyr, etoxazole, fenpyroximate, flufenoxuron, milbemectin, spirodiclofen, tebufenpyrad가 독성이 낮거나 없었다. 살균제와 농약보조제는 모두 낮은 독성을 보였다. 이상의 결과로 보아 시설 병해충의 종합관리체계 에서 으뜸애꽃노린재에 독성이 적은 약제와 으뜸애꽃노린재를 함께 이용할 수 있을 것이다.

Orius strigicollis Poppius를 이용한 총채벌레의 생물적 방제 방법을 구명하기 위하여 하우스 고추에서 천적제거구(CNT), 약제처리구(PAT)와 천적방사구(NRT)의 세처리를 하여 처리구별로 총채벌레와 O. strigicollis의 밀도변동 및 고추 열매의 피해도 조사를 실시하였다. NRT에서 O. strigicollis의 약충은 최초 방사일로부터 13일후에 고추의 하부 잎에서 조사되었으며, 총채벌레의 밀도는 정식 15일후부터 급격히 낮아진 후 CNT보다 매우 낮은 밀도로 유지되었다. 총채벌레와 O. strigicollis가 꽃에 더 많이 분포하기 때문에 포식자 탐색을 증진시키는 결과를 가져왔다. CNT, PAT와 NRT에서 고추 열매의 피해도 지수는 각각 0.31, 0.05와 0.08이었고, 피해과율은 각각 80.0, 17.1과 24.8%로 NRT의 피해도 지수와 피해과율은 PAT보다는 높았으나 CNT에 비해서는 매우 낮아 고추에서 O. strigicollis를 이용하여 총채벌레를 방제할 수 있을 것으로 조사되었다.

총채벌레류의 토착천적인 Orius strigicollis Poppius의 사육에 영향을 주는 부화율, 산란수 및 산란시기, 사육용기 당 암컷 성충의 밀도와 내적자연증가율을 광주기 16L:8D, 상대습도 10%, 온도 의 사육조건에서 조사하였다. 부화율과 알기간은 각각 88.5%, 5.7일이었다. 사육용기 당 산란수는 50-100개일 때에 우화율이 50.8%로 가장 좋았고, 새로 얻은 성충의 회수시기는 산란 후 17일이 적당하였다. 사육용기 당 암컷 성충의 밀도와 새로 얻은 성충의 수와의 관계는 Y= -10.7971n(X)+44.659(=0.7619)와 같았다. 사육실에서 대량으로 사육하는 경우 일세대 평균기간(T), 순번식율( )과 내적자연증가율( )은 각각 26.5일, 6.18과 0.0687이었다.

총채벌레류의 포식성 천적으로 알려진 Orius strigicollis(Poppius)의 발육과 산란에 미치는 온도의 영향을 조사하기 위한 실험조건은 처리온도 15, 20, 25, , 상대습도 60~80%, 광주기 16L:8D로 조절하였으며, 먹이는 목화 진딧물을 급여하였다. 처리온도별 총산란수와 성충수명은 각각 39.1, 66.4, 68.5, 47.5개와 32.6, 26.5, 13.7, 9.0일이었으며, 알기간과 부화율은 각각 18.9, 8.6, 6.1, 3.4일과 70.6, 81.8, 80.0, 91.8%이었고, 약충의 발육기간과 생존율은 각각 45.6, 21.3, 11.6, 9.4일과 26.7, 43.3, 76.7, 46.7%였다. 발육영점온도는 알 , 약충 였고, 유효적산온도는 알 64.9일도, 약충 172.4일도이다.

총채벌레류의 유력천적인 Orius strigicollis의 연중 실내사육에 적합한 대체먹이를 선발하고자 먹이 곤충으로 목화진딧물, 점박이응애, 긴털가루응애를 각각 급여하여 O. strigicollis의 발육과 산란수를 , RH60-80%, 16L:8D 조건에서 조사하였다. 약충기간 중 생존율과 발육기간은 목화진딧물 급여시 81.6%와 11.6일, 점박이응애 급여시 56.7%와 14.6일, 긴털가루응애 급여시 42.3%와 16.5일 이었으며, 성충기간 중 총산란수는 목화진딧물 급여시 68.5개, 점박이응애 급여시 46.1개, 긴털가루응애 급여시 26.5개로 3종의 먹이곤충 중 목화진딧물 급여가 가장 좋은 것으로 나타났다.