In this study, Pleurotus ostreatus No.42 was cultured in glucose-peptone-yeast-wheat bran medium using a previously reported novel rotary draft tube bioreactor. Versatile peroxidase (VP), a lignin-degrading enzyme, was isolated from a pellet-type mycelium culture grown in the medium for seven days. The VP was purified by sequentially applying ultra-filtration, DEAESepharose CL-6B column, and Mono Q column. SDS-PAGE analysis revealed the molecular weight of VP to be 36.4 KDa with an isoelectric point of 3.65. The amino acid sequence was confirmed as VTCATGQTT. The purified VP was observed to possess the property of not only oxidizing Mn ions but also decomposing veratryl alcohol, a non-phenolic compound. The catalytic ability of VP is a subject for future research.

Various types of tanks are used in nuclear power plants, and sludge composed of various organic substances and inorganic oxides contaminated with radioactive materials may be present at the bottom of a tank of a radioactive waste treatment device. In addition, glassy and fixative oxide contamination layers are accumulated on the inner wall of the tank depending on the tank material, usage and degree of oxidation. Such contaminated sludge is the main cause of radiation exposure to workers when dismantling nuclear power plant tanks. In addition, the waste filters generated by filtration of contaminated sludge is treated as secondary radioactive waste, and this radioactive waste not only occupies a lot of disposal space, but also the disposal cost is continuously increasing. Therefore, it is necessary to develop a technology that does not generate waste filters as much as possible. To solve this problem, NILEPLANT Co., Ltd. registered a patent named “Filtering apparatus” based on previous research and manufactured a rotary filtration membrane device through detailed design. The rotary filtration membrane device is composed of three or more multiple rotary filtration membranes, and can remove fine particles in wastewater as well as sludge accumulated inside a radioactive contamination tank. In addition, considering the site characteristics of special conditions such as nuclear power plants, it was designed to show excellent performance in removing fine particles while minimizing the area where the device is installed. The rotary filtration membrane device is designed and manufactured as a double cylinder structure that combines a hydro cyclone filter type body and an inner partition wall, and is equipped with a filter cloth-based rotary cylinder filter to process sludge through the filter cloth in addition to inertial. In addition, the patented principle enables self-backwashing without stopping the filtration process, extending the life of the filter and minimizing waste filters. The filtration performance, self-backwashing function, and sludge behavior of the rotary filtration membrane device manufactured based on the detailed design were evaluated through experiments, and improvements to obtain more effective filtration performance were derived. Accordingly, it is expected that the more improved rotary filtration membrane device can be effectively used to remove sludge generated during the dismantling of nuclear power plants in the future.

Thermoelectric materials and devices are energy-harvesting devices that can effectively recycle waste heat into electricity. Thermoelectric power generation is widely used in factories, engines, and even in human bodies as they continuously generate heat. However, thermoelectric elements exhibit poor performance and low energy efficiency; research is being conducted to find new materials or improve the thermoelectric performance of existing materials, that is, by ensuring a high figure-of-merit (zT) value. For increasing zT, higher σ (electrical conductivity) and S (Seebeck coefficient) and a lower к (thermal conductivity) are required. Here, interface engineering by atomic layer deposition (ALD) is used to increase zT of n-type BiTeSe (BTS) thermoelectric powders. ALD of the BTS powders is performed in a rotary-type ALD reactor, and 40 to 100 ALD cycles of ZnO thin films are conducted at 100oC. The physical and chemical properties and thermoelectric performance of the ALD-coated BTS powders and pellets are characterized. It is revealed that electrical conductivity and thermal conductivity are decoupled, and thus, zT of ALD-coated BTS pellets is increased by more than 60% compared to that of the uncoated BTS pellets. This result can be utilized in a novel method for improving the thermoelectric efficiency in materials processing.

In this study, a study on the design of the rake shape of a rotary screener is conducted. The rake bar and rake of a rotary screener were designed, and three types for the rake shape were designed by changing the rake shape. Structural analysis was conducted in consideration of the vertical load applied when the narrow object was pulled up by the rake and the friction force applied underwater, and the more efficient type was verified by comparing the stress and strain applied to the lake by shape. In addition, structural analysis was additionally performed by changing the material of the rake to a carbon composite material in the same manner. That is, in order to improve the need to replace the entire rake part in case of damage, we will examine the improvement of the strength of the rake by performing structural analysis by changing the material of the rake.

리그닌 분해효소의 생산은 나선형 리본이 있는 새로운 형태의 회전식 통풍관 생물 반응기(RTB)를 사용하여 느타리(Pleurotus ostreatus) No.42에 의해 실시하였다. 락게 이즈(laccase)의 최대 생산량은 배양 3일 후 약 8,200 U/ bioreactor 수준에 도달한 후 감소하였다. 반면에, 망간퍼 옥시데이즈(MnP)의 최대 생산은 6일 배양 후 약 8,400 U/bioreactor의 수준에 도달하였다. 그러나 이 발효조에서 리그닌퍼옥시데이즈(LiP)는 검출되지 않았다. 그 결과 회전식 통풍관 생물 반응기(RTB)가 리그닌 분해효소를 대규모 생산을 위해 성공적으로 생산할 수 있음을 보여주었다. 이 발효기에서 망간퍼옥시데이즈의 정제 과정은 Sepharose CL-6B, Superdex 75 prep grade 및 Mono-Q에 대한 크로마토그래피를 포함하여 정제하였다. 이 주요 효소는 sodium dodecyl sulfate-polyacrylamide 겔 전기영동(SDS-PAGE)에서 분자량 36,400, pI 3.95의 등전점(IEF)으로 각각 확인되었다. 이 발효기의 주요 효소 N-말단 서열은 정치 및 진탕배양과 같은 다른 배양조건에서 보고된 MnP3 효소와 동일하였다.

The SnSe single crystal shows an outstanding figure of merit (ZT) of 2.6 at 973 K; thus, it is considered to be a promising thermoelectric material. However, the mass production of SnSe single crystals is difficult, and their mechanical properties are poor. Alternatively, we can use polycrystalline SnSe powder, which has better mechanical properties. In this study, surface modification by atomic layer deposition (ALD) is chosen to increase the ZT value of SnSe polycrystalline powder. SnSe powder is ground by a ball mill. An ALD coating process using a rotary-type reactor is adopted. ZnO thin films are grown by 100 ALD cycles using diethylzinc and H2O as precursors at 100oC. ALD is performed at rotation speeds of 30, 40, 50, and 60 rpm to examine the effects of rotation speed on the thin film characteristics. The physical and chemical properties of ALD-coated SnSe powders are characterized by scanning and tunneling electron microscopy combined with energy-dispersive spectroscopy. The results reveal that a smooth oxygenrich ZnO layer is grown on SnSe at a rotation speed of 30 rpm. This result can be applied for the uniform coating of a ZnO layer on various powder materials.

The purpose of this study is to analyze the distribution characteristics of mist spray particle size by devising a rotary mist spraying device to develop the evaporative salt water desalination system. The rotary mist spraying device was consisted of a BLDC sirocco fan, a spinning fan, a fan fixed shaft and a salt water supply device etc. In this study we analyzed the characteristics of spray particle size and distribution according to the variation of sirocco fan surface roughness(Ra, μm), revolutions(rpm) and salt water flow rate(mL/min). When sirocco fan surface roughness( Ra) was in the range of 0.27~7.65 μm, the spray particle size was 0.117~1.360 μm. And then more than 90% of spray particles were found to be less than 0.50 μm. When sirocco fan surface roughness(Ra) was in the range of 12.70~22.84 μm, the spray particle size was 2.51~184.79 μm and more than 98% of spray particles were found to be less than 13.59 μm. To analyze the effect of fan rotation speed on the size and distribution of spray particles, when surface roughness Ra was fixed 0.27 μm and fan rotation speed and salt water flow rate was respectively changed at 3,800~5,600 rpm and 2.77~8.28 mL/min, spray particle size was 0.341~0.541 μm. And when salt water flow rate was 9.74 mL/min and fan rotation speed was 3,800~5,200 rpm, spray particle size was in the range of 29.29~341.46 μm and in case of 5,600 rpm more than 98.23% of spray particles were in the range of 2.51~13.59 μm.

Conventional rotary sawing machine for cutting lumber generates severe amount of dust scattering to the environment. In this research, the design improvement of the rotary sawing machine is achieved to significantly reduce the dust scattering by the design process utilizing computational fluid dynamics (CFD) analysis. Several design candidates for the design improvement of the rotary saw system were proposed and modeled, and CFD analyses were performed to choose the best design in viewpoint of the least dust scattering. CFD analysis proved to be very useful to predict the characteristics of the air flow inside the saw system. The movement of dust particles with the air flow during the sawing process was analyzed for various design features of the saw system. The most efficient design to minimize the amount of dust particles ejected from the saw system was chosen based on the CFD analysis results. Then, the prototype of the best candidate of the improved rotary saw machine was built and the amount of dust particles were measured to verify its performance.

In the agriculture of strawberry cultivation, it is necessary to loosening and breaking up of the intertwined coco peat and strawberry root in order to cultivate strawberry again. The main objective of this study is to design and analyze the structure of strawberry rotary device for using breaking up of coco peat and strawberry root. In order to perform crushing coco peat on the bed, the rotary device was designed under the weigh 20kg with the speed 11.75m/min, and it can operate on bed width from 250~310mm. Due to different depth of bed, the body of device also was designed screw holder to adjust the hight from 0~120mm. To evaluate the safety of structure, body device was analyzed in static and free vibration state by using Abaqus program. The device was applied maximum load 1177.2N, and the maximum equivalent stress was reached 41.9MPa. The free vibration analysis of two rotating cutter showed minimum natural frequency mode 338.58Hz and 339.9Hz. The results indicate that the designed rotary device was satisfied and has enough strength under design and simulation conditions.

In order to apply rotary atomizer to agricultural spraying system, the motor direct coupled rotary atomizer was proposed. The effect of operating conditions such as atomizing air flow rate, working fluid flow rate, and rotation speed of spinning cup on the atomization performance was investigated for the proposed direct coupled rotary atomizer. The motor speed was controlled in the range of 6,000 to 12,000 rpm using an alternating current transformer, and the atomizing air was supplied by the compressor. In this study, LDPA was used to analyze the spray characteristics of the rotary atomizer. The representative particle diameters of D10, SMD, MMD, D90, and DMax tended to decrease as the atomizing air flow rate and the motor speed increased, but increased as the working fluid flow rate increased. Also, SMD was found to be influenced by order of atomizing air flow rate> motor speed> working fluid flow rate, and DMax was influenced by order of operating fluid supply> motor speed> atomizing air flow rate.

The new rotary friction damper was developed using several two-nodal rotary frictional components with different clamping forces. Because of these components, the rotary friction damper can be activated by building movements due to lateral forces such as a wind and earthquake. In this paper, various dependency tests such as displacement amplitude, forcing frequency and long term cyclic loading were carried out to evaluate on the structural performance and the multi-slip mechanism of the new damper. Test results show that the multi-slip mechanism is verified and friction coefficients are dependent on displacement amplitute and forcing frequency except long term cyclic loading.

Various hybrid dampers have been developed in Korea to control the vibration due to a wind and earthquake. In order to minimize the installment space, cost and construction process, the new hybrid friction damper is developed. This hybrid damper is composed of several rotary friction components having two frictional joint. Because of these components, the building vibration due to wind and earthquake can be mitigated by hybrid friction damper. In this paper, various dependency tests were carried out to evaluate on the structural performance of two joint rotational friction component of the hybrid damper. Test results show that two joint rotational components do not depend on a displacement and a frequency of forcing but friction coefficients is reducing as a clamping force is increasing.

In this study variable radius pulley is proposed, and the proposal should be applied to pulley of CVT. Variable radius pulley consists of two disks and sliding pins. Phase difference of two disks make a change pitch diameter. Therefore change speed is accomplished by changing pitch diameter of pulley. In this paper simulation for slot variation of rotary disk of the variable radius pulley was performed. Softwares for simulation are Pro/Engineer5 and Hypermesh11.0. The results of simulation are appropriate to put to practical use of the variable radius pulley