This study deals with the application of an artificial neural network (ANN) model to predict power consumption for utilizing seawater source heat pumps of recirculating aquaculture system. An integrated dynamic simulation model was constructed using the TRNSYS program to obtain input and output data for the ANN model to predict the power consumption of the recirculating aquaculture system with a heat pump system. Data obtained from the TRNSYS program were analyzed using linear regression, and converted into optimal data necessary for the ANN model through normalization. To optimize the ANN-based power consumption prediction model, the hyper parameters of ANN were determined using the Bayesian optimization. ANN simulation results showed that ANN models with optimized hyper parameters exhibited acceptably high predictive accuracy conforming to ASHRAE standards.

V. parahaemolyticus causes waterborne and foodborne disease such as acute diarrhea. In this study, V. parahaemolyticus isolates from seawater, fish tanks, and distributed fishery products in Jeju were investigated for potential toxin or species-specific genes (tdh, trh, tlh, and toxR) using RT-PCR and their genetic characteristics were analyzed using Pulsed-field gel electrophoresis (PFGE). Overall, V. parahaemolyticus of 90 strains (36.7%), including 33 strains from seawater, 8 strains from fish tanks, and 50 strains from fishery products, were isolated from 245 samples. All V. parahaemolyticus strains did not detect the tdh gene, whereas all strains detected tlh or toxR genes. In addition, trh genes were detected in 3 strains from seawater and 1 strain from fishery products. Monthly quantitative testing of seawater revealed that V. parahaemolyticus was positively correlated with water temperature. The 90 strains of V. parahemolyticus obtained in this study showed by gene homology between types, ranging from 64.0–97.3%. Among these, thirteen types showed 100% homology between genes. These results indicate that continuous monitoring is needed to facilitate food poisoning epidemiological investigations because some isolated V. parahaemolyticus strains harbored toxin genes and V. parahaemolyticus strains isolated from seawater, fish tanks, and distributed fishery products showed genetic similarity.

Due to red tide and abnormal currents that take place every year, the damage of inland fish farms that takes sea water is increasing frequently. Also, the households of inland fish farm due to the increase in the cost of fuel and electricity are struggling many hardships economically. In relation to underground seawater for it’s farm analyzed economic effect based on the actual conditions for 1,700 inland fish farms, from this I have deduced policy implications for direction this project to be pursued from now on. The saving rate of costs of the fuel and electricity through the development of an underground seawater were approximately analyzed to decline about 3.4〜9.7%, if utilizing only about 5% of the undeveloped farms, it was estimated effect of about 61 billion won, if 10%, it was estimated effect of about 122 billion won. Consideration in selecting the developer of underground seawater will be selected an character of farm method. It is expected this study will be helpful for business analysis related government in the fisheries.

Distribution of pathogenic vibrios in the seawater of live fish tank and effect of environmental factors an their existence were investigated by collecting samples from fish markets and restaurants in 6 different cities. Pathogenic vibrios and coliforms were determined by using the most probable number (MPN) procedure, and aerobic plate count was enumerated by the standard pour plate method. No Vibrio cholerae O1 was detected in all the samples tested. Detection rates of Y. cholerae non-Ol, V. parahaemolyticus and V. vulnificus in all the samples tested were 7.7%, 69.2% and 23.1%, respectively. Water temperature and turbidity of the seawaber measured were higher in the pathogenic vibrios positive samples than in those negative samples. However, higher salinity and pH were shown in the pathogenic vibrios negative samples than in positive samples. The aerobic plate counts and MPN of total and fecal coliforms in the seawater were higher in the presence of pathogenic vibrios than in the absence of pathogenic vibrios. In this study, the presence of pathogenic vibrios in the seawater tested was closely related with other physiochemical parameters and populations of coliforms, indicators for food safety.

DNA damage such as genotoxicity was identified with comet assay, which blood cell of a marine parrot fish (Oplegnathus fasciatus) was exposed to an acidified seawater, lowered pH gradient making of CO2 gas. The gradient of pH were 8.22, 8.03, 7.81, 7.55 with control as HBSS solution with pH 7.4. DNA tail moment of fish blood cell was 0.548 ± 0.071 exposed seawater of pH 8.22 condition, on the other hand, DNA tail moment 1.601 ± 0.197 exposed acidified seawater of pH 7.55 lowest condition. The approximate difference with level of DNA damage was 2.9 times between highest and lowest of pH. DNA damage with decreasing pH was significantly increased with DNA tail moment on blood cell of marine fish (ANOVA, p < 0.001). Ocean acidification, especially inducing the leakage of sequestered CO2 in geological structure is a consequence from the burning of fossil fuels, and long term effects on marine habitats and organisms are not fully investigated. The physiological effects on adult fish species are even less known. This result shown that the potential of dissolved CO2 in seawater was revealed to induce the toxic effect on genotoxicity such as DNA breakage.

Carbonic anhydrases catalyze the hydration of carbon dioxide and are essential for regulation of cellular pH and carbon dioxide transport. CA is the zinc metalloenzyme that catalyses the reversible reactions of CO2 with water. To date, 16 isozymes belonging to the α-CA gene family. A carbonic anhydrase isozyme is present in shaggy sea raven, Hemitripterus villosus. Carbonic anhydrase isozyme protein isolated from several tissues showed specific protein band with molecular weight of 30 kDa. The isolated protein band was identified as a carbonic anhydrase isozyme.