The cutaneous tolerability of detergent formulations can be improved by means of suitable additives. They complex the surfactant molecules lowering the concentration of their free monomeric species. Proteins derivatives used as additives for detergency are usually prepared by partial hydrolysis of plant reserve proteins. The main purpose of the hydrolytic cleavage is to make them water soluble and suitable for liquid products. Water solubility and stability are obtained by means of complexation with surfactants which also increase their actual hydrophobicity, an important parameter affecting cosmetic properties of proteins. Transepidermal water loss (TEWL) and electric capacitance (EC) have been adopted as investigation technigues to evaluate the skin integrity/damage in vitro tests, The performance of native wheat protein / surfactant complexes has been compared with traditional protein hydrolysates as detergent additives. The results show a noticeable reduction of skin irritation in surfactant formulations with addition of native wheat protein.

Genetic variations of γ-/ω-gliadin and Spa (storage protein activator) in 40 Korean wheat cultivars were evaluated to provide genetic information for improving end-use quality in wheat breeding programs. Korean wheat cultivars were classified into 13 patterns at the Gli-1 locus based on the allelic variation using A-PAGE (acidic-polyacrylamide gel electrophoresis). Seven, five, and six alleles were identified at Gli-A1, Gli-B1, and Gli-D1 loci, respectively. Allele-specific PCR markers for γ-gliadin corresponded to specific allele at Gli-1 loci on A-PAGE, which Gli-A1f, Gli-A1h and Gli-A1l alleles corresponded to GliA1.2, Gli-B1h and Gli-B1f alleles corresponded to GliB1.2 and Gli-D1f, Gli-D1m and Gli-D1o alleles corresponded to GliD1.1. DNA markers for γ-45 and γ-42 also corresponded to the γ-gliadin patterns around 40kDa on A-PAGE, except in Sukang, Ol and Joongmo2003. However, allelic specific PCR markers for ω5-gliadin did not correspond to that of A-PAGE. Three alleles were identified at Spa-A1 locus, whereas there was no variation at Spa-B1 and Spa-D1 loci.

농촌진흥청 국립식량과학원에서는 용도별 품종의 다양화를 위해 답리작 적응 과자용 밀 품종 ‘고소’를 개발하였다. 고소밀은 1998년도에 연질인 고분밀을 모본으로 하고, 숙기가 빠른 올밀을 부본으로 인공교배하여 SW98148 조합을 육성하였다. 경기도 연천에서 집단재배 후 계통을 전개하여 초형과 수형이 양호하며 내한성이 강한 계통인 SW98148-YB-9-2-4-1-3-1을 2006년부터 2개년간 생산력 검정을 거쳐 ‘익산 320호’로 계통명을 부여하였다. 그 후 2008년부터 3개년 동안 지역적응시험을 실시한 결과 숙기가 빠르며 수량성이 높고, 과자적성이 우수하여 2010년 농작물 직무육성 신품종심의회에서 ‘고소’로 명명하였다. 고소밀의 파성은 Ⅱ형으로 춘파형이고, 엽색은 녹색이며 이삭은 방추형이고, 종자는 중간 크기로 적색이다. 출수기와 성숙기는 밭상태 조건에서 각각 4월 28일과 6월 7일, 논상태에서는 각각 4월 25일과 6월 4일로 비슷하다. 고소밀의 리터중(799 g/L)과 천립중(42.5 g) 은 금강밀(804 g/L과 46.7 g)보다 낮고, 내한성(4.4%)과 수발아(4.0%) 저항성이 강하고, 내도복성이 높다. 고소밀의 제분율(63.77%), 단백질(9.8%), 침전가(30.0 ml)과 글루텐(7.2%) 함량은 금강밀(73.6%, 12.6%, 50.0 ml과 10.8%)보다 낮고, 과자적성이 우수하였다. ‘고소밀’의 HMW-GS 조성은 Glu-A1에서 2*, Glu-B1에서 7+8을 나타내 금강밀과 조성이 같았으나, Glu-D1에서 2.2+12가 발현되었다. GBSS 조성은 금강밀과 같은 야생형이고, Pinb-D1은 야생형으로 금강밀과 다른 특성을 나타냈다. 지역적응시험에서 밭상태 수량은 657 kg/10a, 논상태 수량은 561 kg/10a로 금강밀보다 21%와 7% 증수하였다.

Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) affects bread and noodle processing quality, the function of specific LMW-GS proteins mostly remain unclear. It is important to find a corresponding gene for a specific LMW-GS protein in order to understand the function of the specific LMW-GS protein. The objective of this study was to identify LMW-GS genes and haplotypes using well known Glu-A3, Glu-B3 and Glu-D3 gene specific primers and to interlink their protein products by proteomic approaches in a wheat variety. A total of 36 LMW-GS genes and pseudo-genes were amplified including 11 Glu-3 gene haplotypes, designated as GluA3-13K and GluA3-22K (pseudogene) at Glu-A3 loci, GluB3-33K and GluB3-43K at Glu-B3 loci and GluD3-11K, GluD3-21K, GluD3-31K, GluD3-42K, GluD3-5K, GluD3-6K and GluD3-393K (pseudogene) at Glu-D3 loci. To determine the relationship between gene haplotypes and their protein products (to identify the corresponding LMW-GS proteins), we conducted N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS) analysis of the 17 LMW-GS spots separated by 2-DGE. Successfully, LMW-GS proteins of the Glu-3 gene haplotypes except pseudo-genes mentioned above were identified. This is the first report on comprehensive characterization of LMW-GS genes and their corresponding proteins and establishment of specific correspondence between each other in a single wheat cultivar. Our approach will be useful to understand the molecular basis of the LMW-GS and to study their contribution to the end-use quality of flour.

Rice flour is used in many food products. However, dough made from rice lacks extensibility and elasticity, whereas that of wheat is suitable for many food products including breads. We have produced marker-free transgenic rice plants containing a wheat TaGlu-Ax1 gene encoding the HMG-GS from the Korean wheat cultivar ‘Jokyeong’ using the Agrobacteriummediated co-transformation method. The TaGlu-Bx7-own promoter was inserted into a binary vector for seed-specific expression of the TaGlu-Ax1 gene. Two expression cassettes comprised of separate DNA fragments containing only TaGlu-Ax1 and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to the Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring TaGlu-Ax1 or HPTII was infected to rice calli at a 3:1 ratio of TaGlu-Ax1 and HPTII, respectively. Then, among 210 hygromycin-resistant T0 plants, we obtained 20 transgenic lines with both TaGlu-Ax1 and HPTII genes inserted into the rice genome. We reconfirmed integration of the TaGlu-Ax1 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the wheat TaGlu-Ax1 were stably expressed in the rice T1 seeds. Finally, the marker-free plants harboring only the TaGlu-Ax1 gene were successfully screened at the T1 generation.

Development of transgenic plant increasing crop yield or disease resistance is good way to solve the world food shortage. However, the persistence of marker genes in crops leads to serious public concerns about the safety of transgenic crops. In the present study, we developed marker-free transgenic rice inserted high molecular-weight glutenin subunit (HMW-GS) gene (Dx5) from the Korean wheat cultivar ‘Jokyeong’ using Agrobacterium-mediated co-transformation method. The Dx5’s own promoter was used for protein expression. Two expression cassettes comprised of separate DNA fragments containing only the Dx5 and hygromycin resistance (HPTII) genes were introduced separately into Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Dx5 or HPTII was infected into rice calli at a 3: 1 ratio of EHA105 with Dx5 gene and EHA105 with HPTII gene expressing cassette. Then, among 270 hygromycin-resistant transformants, we obtained 27 transgenic lines inserted with both the Dx5 and HPTII genes into the rice genome. We reconfirmed integration of the Dx5 gene into the rice genome by Southern blot analysis. Wheat Dx5 transcripts in T1 rice seeds were examined with semi-quantitative RT-PCR. Protein expression of the Dx5 was analyzed with Western blot using polyclonal antibody recognising x-type of glutenin subunits in T1 seeds. It was suggested that the protein-processing system was conserved between rice and wheat. Finally, the marker-free plants containing only the Dx5 gene were successfully screened at the T1 generation.

This study was conducted to compare the protein characteristics, dough rheology and bread loaf volume of Korean wheat cultivars and CIMMYT lines produced in diverse environments and to determine the genetic and environmental effects on bread making quality. Protein characteristics, including protein content and SDS-sedimentation volume, mixing properties during dough development and bread loaf volume were primarily influenced by the environment. Wheat cultivated in Jinju exhibited higher SDS-sedimentation volume based on constant protein weight and bread loaf volume than those in Suwon and Iksan. SDS-sedimentation volume based on constant protein weight, mixing time of mixograph and mixing tolerance of mixograph were positively correlated with bread volume. Korean wheat cultivars showed different allelic variations of Glu-1 and Glu-3 compared to CIMMYT wheat lines. Alchanmil, Keumkangmil and Tapdongmil could be suitable for bread making because these cultivars exhibited a 10 point Glu-1 score. However, Korean wheat cultivars should be introduced specific alleles in Glu-3 loci, including Glu-A3b or d and Glu-B3b , d , f or g , to improve gluten strength related to increase bread loaf volume.

미성숙 종자로부터 추출된 전체 RNA를 이용하여 합성한 cDNA와 LMW-GS 특이 프라이머세트를 이용하여 43개의 LMW-GS 유전자를 분리하였다. 각각의 유추 아미노산은 상동성이 높은 20개의 시그널 펩타이드, N-말단 영역, 반복서열영역 그리고 C-말단 영역을 가지며 C-말단 영역에 분자내 혹은 분자간 이황화 결합을 형성하는 전형적인 8개의 시스테인을 가지고 있었다. 이들 시스테인의 위치는 첫번째, 일곱번째를 제외하고는 보존되어 있었다. Ikeda

Pectin, one of the main components of plant cell wall, is deesterified in muro by PME (Pectin methylesterase). PME activity is particularly regulated by inhibitor proteins known as the pectin methylesterase inhibitor (PMEI). The PMEI plays a key role in wounding, osmotic stress, senescence and seed development. However, the role of PMEI in plant species still remains to be demonstrated especially in wheat. To facilitate the studies on the expression of the TaPMEI gene, RT-PCR was performed using leaf, stem and root tissues in response to exogeneous application of phytohormones and abiotic stress treatments. Transcription of the TaPMEI gene was significantly induced in NaCl, H2O2 and SA treatments, and reduced when plants were treated with ABA. To elucidate the subcellular localization of the TaPMEI protein, TaPMEI:GFP fusion construct was transformed into onion epidermal cells by particle bombardment. The fluorescence signal was exclusively detected in cell wall of the cells. In order to obtain recombinant TaPMEI protein, the TaPMEI protein, expressed in E.coli as a MBP (~42.5 kDa) fusion protein recombinant. Purification and functinal analysis of TaPMEI as an inhibitor of PME activity are described.

The wheat-rye translocation lines have been agriculturally developed for the resistance to the biotypes of Hessian fly as a major insect pest of wheat. In order to compare the proteomic profiles between ‘Coker797’ (non-2RL), ‘Hamlet’ (2RL), and near-isogenic line (NIL) carrying 2RL, we evaluated the protein extraction and preparation methods for two-dimensional gel electrophoresis approach. The tissues such as leaves, stems, and roots from three wheat-rye lines were extracted by following trichloroacetic acid (TCA)/acetone precipitation. In a preliminary proteome analysis, a commonly expressed protein in Hamlet and NIL strain was identified as methionine synthase annotated in Hordeum vulgare subsp. The present study will provide the experimental guideline for the proteomic study of other useful crop plant tissues.

Systemic acquired resistance is an important component of the disease resistance repertoire of plants. A novel syntheticchemical, Benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH), was shown to induce acquired resistance in wheat.BTH prote