This study aimed to optimize the enzymatic process for extracting narirutin from yuzu pomace while maximizing yield, and to assess its functional properties related to prebiotic, antimicrobial, and anti-allergic activities. Given the high pectin content in yuzu pomace, which increases extract viscosity and impedes the release of bioactive compounds, a sequential enzyme treatment was implemented. Pectinase (P4) was first used to degrade pectin, followed by cellulase (C1) to facilitate the release of narirutin. The P4–C1 extract achieved a 2.17-fold increase in narirutin content compared to the crude extract without enzyme treatment. In prebiotic activity, the P4–C1 extract enhanced the growth of Lactobacillus plantarum and Bifidobacterium infantis by 34.62% and 28.03%, respectively, compared to the crude extract. Additionally, the P4–C1 extract demonstrated improved antimicrobial activity against Salmonella typhimurium, Staphylococcus epidermidis, and Staphylococcus aureus, with increases of 22.69%, 27.09%, and 25.04%, respectively. Furthermore, in anti-DNP-IgE-sensitized RBL-2H3 cells, the P4–C1 extract showed enhanced anti-allergic activity by reducing β-hexosaminidase release by 16.22% compared to the crude extract. These findings indicate that the optimized enzymatic process significantly improves narirutin extraction efficiency and that the extract has considerable potential as a functional ingredient for prebiotic, antimicrobial, and anti-allergic applications.

This study analyzed the texture and rheological properties of waxy barley flour subjected to heat-moisture treatment and α-amylase treatment and examined how these treatments affect the processing suitability. Waxy barley flours were treated with a heat-moisture treatment (HM), α-amylase treatment (EZ), and their combined treatment (HM-EZ), and the transparency, textural properties, steady shear flow, and dynamic viscoelastic characteristics were analyzed. The HM and HM-EZ treatments maintained low transparency from the early storage stage and exhibited higher hardness and gel strength, suggesting enhanced gel-forming characteristics, as well as higher shear stress and apparent viscosity under shear conditions. In contrast, the EZ-treated samples showed improved initial dispersibility, increased adhesiveness and extensibility, and lower viscosity with higher flowability. Dynamic rheological analysis revealed elastic-dominant gel behavior in the HM and HM-EZ treatments, whereas the EZ treatment resulted in a lower storage modulus and more flexible viscoelastic behavior. These results show that the physical and rheological properties of waxy barley flour vary with the heat-moisture and α-amylase treatments, and suggest that tailored processing strategies can be used to design waxy barley-based food ingredients for specific applications.

This study explored how enzyme type (cellulase and pectinase), concentration, and mixing ratio influence the functional compounds and antioxidant activities of purple sweet potato (Ipomoea batatas L.). The enzyme treatment significantly impacted all measured parameters. At a cellulase concentration of 30 units, the total anthocyanin content reached 399.4 mg/100g dry weight (DW), representing a 163% increase compared to the control (151.6 mg/100g DW). Total polyphenol (1,799.7 mg GAE/100g DW) and flavonoid (475.3 mg CE/100g DW) contents also peaked under these conditions, while higher enzyme concentrations or increased pectinase ratios reduced extraction efficiency. The highest ABTS radical scavenging activity was noted at 30 units of cellulase (954.0 mg TE/100g DW) and at 80 units in a combined treatment of cellulase (2) to pectinase (1) (927.2 mg TE/100g DW), suggesting that moderate enzyme synergy enhances antioxidant release. Conversely, DPPH activity decreased with higher enzyme concentrations, with all combined treatments yielding lower results than the control. Overall, the optimal condition for enhancing both functional compound extraction and antioxidant activity in purple sweet potato was identified as a single treatment with cellulase at 30 units.

본 연구는 효소 처리 방법을 적용하여 제조한 생강 착즙박 추출물 의 수용화 특성 및 유용성분 추출 수율을 증진시키는 최적 효소 처리 조건을 선정하고, 대용량 생산 적용 가능성을 확인하고자 하였다. 효 소 처리에 사용한 효소는 세포벽 분해효소인 P와 전분분해효소인 T 를 이용하였고, P 처리 방법 및 가수 방법을 변형하여 진행하였다. 효소 처리 및 추출 조건에서는 CON 대비 GP3에서 WSI는 2배 이상 증가하였고, TP 및 TF 함량은 각각 1.6, 1.4배 증가하였다. 6-G 및 Total 함량은 1.7배 증가하는 것으로 나타났다. 효소 전처리 방법에 서는 GP3에서 TP 함량 값이 가장 높았고, 6-G 및 Total 함량 또한 유의적으로 가장 높게 나타났다. 효소 전처리 시간에서는 P를 24시 간 전처리했을 때 TP, TF, 6-G 및 Total 함량에서 유의적으로 가장 높게 나타나 효소 전처리 최적 조건을 선정하였다. 선정된 효소 전처 리 기술(25℃, 24시간)을 활용하여 생강 착즙박 추출물을 용량별(0.1, 15 kg)로 제조하여 비교한 결과, WSI는 CON 대비 효소 전처리구 (GP3)와 효소 처리구(GP1) 모두 증가하였고, 처리 용량에 따라서도 같은 경향을 나타냈으며 GP3가 가장 높았다. WAI는 대용량으로 처 리했을 때 GP3가 가장 낮았다. TP는 소용량 및 대용량 모두 CON 대비 GP3가 가장 높았고, TF는 소용량에서는 CON 대비 GP3가 가 장 높았지만, 대용량에서는 차이가 없었다. 6-G 측정 결과 소용량보 다 대용량으로 처리했을 때 GP3, GP1 모두 유의적으로 증가하였고, Total 함량은 소용량에서는 CON 대비 GP3가 1.7배 높아졌으며 대용 량에서는 약 1.3배 증가하였다. 결과적으로 생강 착즙박에 효소 전처 리 방법을 적용하는 것은 수용화 증진 및 유용성분 추출 함량을 증대시킬 수 있는 단순 공정의 유용한 기술로 사용될 수 있을 것이라고 판단된다. 또한 용량별 효소 처리 추출물의 기능성분 함량 비교결과 를 통해 생강 착즙박의 산업적 대량 생산을 위한 추출 공정 적용 가 능성을 확인하였으며, 이를 통해 부가가치 향상 및 기능성 소재로서 의 가능성을 제시하였다.

Hydrolyzed proteins have an advantage over intact proteins in terms of their rate of digestion and absorption. The high-pressure enzymatic extraction (HPE) method has been shown to improve the quality characteristics of hydrolysates from Protaetia brevitarsis seulensis (Kolbe) larvae (PBSL). This study investigated the effects of the HPE treatment period, a key candidate factor, on the quality characteristics of PBSL HPE hydrolysates. The hydrolysates were prepared by HPE for 0, 12, 18, 24, 30, and 36 h under optimized conditions—solid:solvent ratio (1:14 [w/v]), using complex proteases (Alcalase:Flavorzyme:Bromelain = 1:1:1, 4%), treatment temperature (50oC), and pressure level (100 MPa). All quality characteristics tended to be superior with longer HPE treatment periods, most of which had the highest values at 30 h, with no significant difference or a slight decrease after that. The quality characteristics of the PBSL HPE hydrolysates were improved by 1.3-1.7 times under conditions of optimal HPE treatment period.

This study was performed to determine the total anthocyanin contents, DPPH radical scavenging activity, and SODlike activity of extracted anthocyanins from Bokbunja for analyzing the optimal conditions of enzyme treatment by response surface methodology (RSM) based on three factors: concentration of Pectinex Ultra SP-L, reaction temperature, and reaction time. The optimal enzyme treatment conditions of Pectinex Ultra SP-L concentration, reaction temperature, reaction time, and overall desirability by analysis of multiple response surface methodology with the same weighed value of each dependent variable were 0.5 mL/100 L, 20oC, 2 hr, and 0.63, respectively. Total anthocyanin content, DPPH radical scavenging activity, and SOD-like activity of the conditions stated above were 5.2 mg/ 100 g, 61.3% and 30.5%, respectively. Meanwhile, those of the control were 1.1 mg/100 g, 11.8% and 1.1%, respectively.method.

The effects of ultrasound and enzyme treatments on reducing sugar production from Korean 6-row barley Dahyang during the mashing process of brewing were studied. The concentration of reducing sugar produced by the traditional method was 41.5 mg/mL. The treatment of both the ultrasound at 400 W and amyloglucosidase (0.1 U/mL) increased the reducing sugar concentration produced in the mashing process by 15% compared to the traditional method. In addition, both treatments reduced the total mashing time from 115 min to 70 min. The combination of ultrasound and amyloglucosidase treatment increased the reducing sugar production yield by 17% during mashing, in comparison with the traditional method.

천연조미소재 개발을 위하여 고압/효소분해 시스템에서 멸치 단백질의 분해 품질특성을 탐색한 결과, 최적 조건은 효소농도 0.6%, 온도 50oC, 처리시간 24시간 및 압력 50 MPa로 확인되었다. 멸치 단백질의 처리방법에 따른 품질특성을 비교한 결과, 최적조건하에서 고압/효소 처리한 멸치 가수분해물의 품질특성이 가열추출물인 대조구에 비하여 2.8배, 2배, 1.4배 증가하여 고압/효소 처리에 의한 단백질 가수분해물 생산은 가열추출법이나 고압반응에 비하여 효율적인 방법으로 나타났다. 효소종류에 따른 분해력은 복합효소로 가수분해한 경우 상업효소에 비하여 큰 증가율을 나타내어 복합효소의 분해력이 상업효소에 비하여 우수하였다. 고압/효소 처리 후의 멸치 가수분해물은 정미성 아미노산으로 알려져 있는 glutamic acid, glycine, arginine 및 alanine 등의 함량이 대조구나 압력 처리구의 유리아미노산 함량에 비하여 증가하였다. 결론적으로 고압/효소분해 처리공정은 멸치 단백질의 효과적 분해와 정미성 아미노산 생산에 효율적인 기술임을 확인하였다.

침엽수와 활엽수 펄프내의 리그닌(lignin) 제거 효과를 개선하기 위해 호알칼리성 균류인 Cephalospotium sp. RYM-202의 xylanase를 표백 전처리하고 이에 의한 펄프의 표백 증진 효과를 조사하였다. 두 종류의 펄프 모두 50℃에서 효소반응을 수행하였을 때 펄프내 xylan의 가수분해가 가장 높게 나타났다. 펄프내 xylan의 가수분해를 위한 효소의 최적 pH는 8.0이었으며, pH 9.0에서도 최대활성의 90%

The establishment of optimal producing condition for rice-based infant foods using modified rice powder was attempted. The modification of rice powder was prepared by microwave heating as well as partial enzymatic hydrolysis. Not only thermal treatment but also enzymatic hydrolysis on rice powder increased D.E. value from 1.25 to 3.81. The water binding capacity, swelling power, solubility, digestibility by α-amylase, light transparency, and paste clarity of the modified rice powder compared to unmodified rice powder were increased from 107% to 249%, from 7.80 to 42.52, from 0.04% to 0.89ft, from 9.19% to 23.01, from 33% to 42%, and from 2.2% to 3.9%, respectively. On the other hand, gelatinization temperature, apparent viscosity, and degree of retrogradation of modified rice powder showed negative correlation with D.E. value. The 「results suggested that the thermal and enzymatic treatment on rice powder improved the physicochemical properties of rice based infant food by enhancing carbohydrate absorptionability and lowering the viscosity and opacity.

In this study, enzyme (thermoase) hydrolysis was applied to the porcine blood order to increase the iron content and solubility. It was confirmed that content of iron was increase up to 158.11 mg/100 g porcine powders after 0.2% thermoase treatment at 60℃ during 4 hr. The solubility of porcine blood powders was higher than other enzyme (various protease), temperature, reaction time. This optimized conditions were also worked to the in vitro iron bioavailability rate increasement, the bioavailability of hydolyzed porcine powders was 3-fold higher than that of an iron supplement on the market. These results indicate the possibility of porcine blood powder in iron supplements market as natural material. Also utilizing of reduced porcine blood will be possible to improve environmental issues.