In this study, we present geometrical and kinematical analysis of Moreton wave observed in 2012 June 3rd and July 6th, recorded in H-α images of Global Oscillation Network Group (GONG) archive. These large-scale waves exhibit different features compared to each other. The observed wave of June 3rd has angular span of about 70◦ with a diffuse wave front associated to NOAA active region 11496. It was found that the propagating speed of the wave at 17:53 UT is about 93180 km/s. The broadness nature of this Moreton wave can be interpreted as the vertical extension of the wave over the chromosphere. On the other hand, the wave of July 6th associated with X1.1 class are that occurred at 23:01 UT in AR NOAA11515. From the kinematical analysis, the wave propagated with the initial velocity of about 994 ± 70 km/s which is in agreement with the speed of coronal shock derived from type II radio burst, v ~ 1100 km/s. These two identified waves add the inventory of the large-scale waves observed in 24th Solar Cycle.
본 연구에서는 수수 등겨에 함유되어 있는 타닌의 새로운 천연 색소자원으로서의 활용 가능성을 알아보기 위해서 추출된 색소의 성분을 조사하였으며, Fe와의 킬레이트 반응 조건과 킬레이트화 색소에 대한 여러 가지 특성이 조사되었다.
수수 등겨로부터 추출된 수용액의 흡수 스펙트럼에서는 자외선 영역인 281 nm에서 타닌에 의한 최대 흡수 peak을 보여주고 있다. pH 조건에 따른 킬레이트화 반응에서는 pH 7.5의 반응 조건이 가장 효과적이었다. Fe 농도 2 mg/L 까지는 킬레이트화 반응이 급격히 증가하나, 그 이상의 농도에서는 더 이상의 추가적인 킬레이트화는 없는 것으로 나타났다
킬레이트화 된 타닌 색소의 입자 크기는 4.5~17 μm, 20~42 μm, 45~80 μm, 83~160 μm 범위의 네 그룹으로 나누어지는 것을 확인 할 수 있었다. DSC 분석에서는 타닌의 킬레이트화에 따라 열분해에 기인한 흡열 peak가 크게 증가(318oC→415oC)한다는 것을 확인 할 수 있었으며, TGA 분석에서도 열분해 시작 온도가 253oC에서 382oC까지 상승하는 것을 알 수 있어, 킬레이트화는 열 안정성을 향상시킨다는 것을 확인하였다.
For small-size reinforce-concrete buildings, Midas Gen, OpenSees, and Perform-3D, which are structural analysis programs that are most popularly used at present, were applied for nonlinear static pushover analysis, and then difference between those programs was analyzed. Example buildings were limited to 2-story frames with irregular shaped walls. Analysis result showed that there were more differences than for frames only and frames with rectangular walls, but it was not so significant. Nevertheless, the capacity curve were different in some buildings, which is attributed to shape and location of walls, and feature of the analysis program. Especially, selection of automatic or manual input in Midas Gen, or nonlinear wall elements in Perform3D can affect the capacity curve and performance of the buildings. Therefore, the program users should understand the feature of the program well, and then conduct performance assessment. The result of this study is limited to low-story buildings so that it should be noted that it is possible to get different results for mid- to high-rise buildings.
기존 구조물의 모델 업데이팅 기법은 주로 진동 계측을 통해 얻은 전역적 구조 응답-모달 파라미터-를 이용한다. 모달 파라미터를 이용하여 업데이트된 모델은 전역적 구조 응답을 잘 추정할 수 있지만, 부재 레벨의 안전성 평가를 위한 국부적 응답 예측에는 어려움이 있다. 구조물 내 구조 부재들의 변형률 계측을 통해 응력을 추정하고, 안전성 평가가 이루어진다. 따라서, 본 연구는 모달 파라미터 이외에 로컬 구조 부재들에 가하는 해머 가력을 통해 계측한 변형률을 추가적으로 모델 업데이팅에 이용한다. 본 연구가 제안하는 모델 업데이팅에서 목적함수는 전역적/국부적 계측 응답과 모델의 응답간의 차로 설정되며 NSGA-II를 이용하여 이를 최소화된다. 업데이트 모델에서 예측하는 변형률 응답은 철골조의 안전성 평가에 활용된다. 제안한 기법은 철골 프레임에 대한 시뮬레이션과 해머 가력 실험을 통해 검증된다.
We introduce the Lee Sang Gak Telescope (LSGT), a remotely operated, robotic 0.43-meter telescope. The telescope was installed at the Siding Spring Observatory, Australia, in 2014 October, to secure regular and exclusive access to the dark sky and excellent atmospheric conditions in the southern hemisphere from the Seoul National University (SNU) campus. Here, we describe the LSGT system and its performance, present example images from early observations, and discuss a future plan to upgrade the system. The use of the telescope includes (i) long-term monitoring observations of nearby galaxies, active galactic nuclei, and supernovae; (ii) rapid follow-up observations of transients such as gamma-ray bursts and gravitational wave sources; and (iii) observations for educational activities at SNU. Based on observations performed so far, we nd that the telescope is capable of providing images to a depth of R = 21:5 mag (point source detection) at 5- with 15 min total integration time under good observing conditions.
Quinto Edward Jay Mansarate. 2015. “Dichotomizing Ideological Representations in President Benigno Aquino III's October 30th Address”. The Sociolinguistic Journal of Korea 23(2). 65~95. On October 30th 2013, Philippine President Benigno S. Aquino III addressed the Filipino people in a 12-minute televised speech, in which he defended the government's spending program, Disbursement Acceleration Program (DAP), against accusations of it being the President's version of lawmakers' Priority Assistance Development Fund (PDAF) or ‘Pork Barrel.’ This paper examined ideological representations through a systematic discourse analysis of the 19-paragraph, 1851-word speech. In particular, the paper examined lexical choices within the bounds of ‘biased lexical items’ in political discourse (van Dijk 2005) and ‘referential strategy’ (Wodak, De Cillia & Reisigl 1999). Analysis of data pointed to the polarization of pronouns representing ‘US’ and ‘THEM.’ Data also revealed that positive-self and negative-others representations were achieved through the use of two strategies in van Dijk's (1998) ideological square: (i) emphasis of good things about ‘US’ and (ii) emphasis of bad things about ‘THEM.’ These strategies framed the President and his administration under good governance and administration accomplishments, while framing the critics and opposition as enemies of good governance and part of a failed past. The President's discursive choices reinforced the underlying ideological implication, ‘the Disbursement Acceleration Program is not pork barrel.’ The paper closes with some implications for future research. (200)
1940년대 이후 아시아와 아프리카의 많은 나라들은 식민지 주둔군의 정치공백 이후 도처에서 수많은 부족 간의 갈등과 분쟁이 발생되어왔다. 부족 간의 갈등은 조기에 수습되고 화해를 이루지 못하면 자칫내전으로까지 확대되어 수백만의 살상을 초래하기도 하였다. 제2차세계대전 이후 세대대전이 아님에도 불구하고 지구촌 곳곳에서 일어난 전쟁과 내전은 150~200여 회에 달하며, 이 과정에서 사망자들은 1차 세계대전의 전사자 840만 명을 웃도는 것으로, 이런 사실은 20세기후반이후 나라와 종족간의 분쟁과 내전이 급증하였음을 보여준다.
이러한 부족갈등은 그 자체로써도 비국일 뿐만 아니라 선교와복음전도를 가로막는 장벽이 된다. 그러므로 선교사는 복음화를 통하여현지 교회를 화평의 사자로 세워야 할 뿐 아니라, 그 자신도 화해를위해 노력해야 한다. 갈등의 원인은 집단의 이익의 상충됨으로 인한집단 이기심의 발로이며, 더 큰 비극을 막기 위해, 그리스도의 복음과 화해 정신으로 그들을 화해하도록 노력해야 한다.
본 논문은 갈등의 원인과 전쟁피해의 고통, 전쟁 예방과 평화를위한 화해사역의 당위성을 논하고, 갈등극복과 화해사역에 대한 신학적당위성을 다룰 것이다. 그리고 갈등의 화해사역에 대한 선교신학적당위성을 다룰 것이다. 그리고 르완다에서의 김평육 선교사의 경우,우간다에서의 최정호 선교사의 실례를 통하여 갈등의 상황에서 복음을 전한 과정과 화해의 과정을 살펴보고자 한다. 이를 통하여 선교와 화해사역간의 관계를 분석하고 갈등지역에서의 선교방안을 제시하고자 한다. 연구 방법으로는 성경신학적 고찰과 선교 현장에 대한 문헌자료와 현장 선교사들의 선교편지와 인터뷰 등을 뒷받침하여 화해사역선교방안의 논증을 시도하고자 한다.
새로운 형태의 포스파젠 진단막을 제조해 혈액 속의 글루코우즈의 농도를 측정하였다. 혈액 속에 존재할 가능성이 높은 장애성분들을 4가지 그룹으로 분류하여 혈당 측정에 미치는 영향을 조사하였다. 가장 낮은 농도를 나타내는 시리즈 I의 경우, 대부분의 첨가물들이 혈당 측정에 큰 영향을 미치지 않았으나, 오직 ascorbic acid (AA)만이 기준 용액보다 5~8% 정도 높은 K/S 결과치를 나타내었다. 시리즈 II의 경우, 네 가지 첨가물 모두 기준 용액의 K/S 결과치보다 3~4% 다소 낮은 값을 보였으나 농도에 따른 일정한 값을 보여 주었다. 시리즈 III의 경우, 세 가지 첨가물 모두 기준 용액에서 얻어지는 K/S 결과치와 큰 차이를 보이지 않았다. 가장 높은 농도를 나타내는 시리즈 IV의 경우, 두 가지 물질 모두가 기준 값보다 11~15% 정도 낮은 수치를 나타내었으나 혈당 측정에 크게 영향을 주지는 않았다.
본 연구에서는 일시적으로 관절염 증상 완화에 효과가 있는 건강기능식품인 Rosa Canina L.의 지표성분인 hyperoside를 분석하였다. 국내·외 분석법과 저널들을 참고하여 hyperoside의 HPLC 분석법을 확립하였다. Hyperoside의 HPLC 분석법은 이동상과 컬럼에 대한 실험을 통하여 Capcell Pak C18 MG II 컬럼으로 353 nm으로 설정하였다. 시험 분석법 검증은 hyperoside에 대한 직선성, 정확성, 정량한계(LOQ) 및 정밀성을 수행하였다. Hyperoside은 2~60 μg/mL 농도에서 우수한 직선성(R2 = 0.999)을 나타내었다. 정확성의 회수율은 98~99%로 관찰되었으며, LOQ는 0.393 μg/mL으로 나타났고, 재현성에 대한 정밀성은 상대표준편차가 0.6~2.6%로 관찰되었다.
목 적: 각막굴절교정렌즈 착용 후 시력의 질 변화를 알아보기 위하여 시력의 질과 객관적 시력을 측정하 고 착용 전과 비교하였다.
방 법: 각막굴절교정렌즈를 처음 처방받은 사람으로 매일 밤에 적어도 8시간 이상 각막굴절교정렌즈를 착용할 수 있으며 굴절이상도가 근시 -6.00 D 이하, 난시 -2.00 D 이하인 45명(85안)을 대상으로 하였다. 굴절검사는 자동굴절검사기를 이용하였고, 시력은 LCD Chart ETDRS를 사용하였다. 시력의 질 평가를 위 해 OQAS(optical quality analysis system II)를 이용하여 MTF(modulation transfer function), Strehl ratio, OSI(objective scattering index)와 객관적 시력 OV(OQAS values)를 측정하였고, 각막굴절교정렌즈 착용 전에는 구면 굴절력과 원주 굴절력을 시험용 안경렌즈로 교정한 후 측정하였다. 검사값은 SPSS 18.0을 이용하여 분석하였으며 유의수준은 p<0.05로 하였다.
결 과: 각막굴절교정렌즈 착용 후 나안시력은 각막굴절교정렌즈 착용 전 0.79±0.24 LogMAR에서 1개월 후 0.03±0.05 LogMAR로 유의하게 개선되었고(p<0.001), OQAS로 측정한 객관적 시력 OV100% 및 OV20%는 렌즈 착용 전 교정시력과 유의한 차이를 보이지 않았으나(p>0.05), OV9%는 착용 전보다 유의하게 저하되었 다(p=0.011). MTF 값과 Strehl ratio는 각막굴절교정렌즈 착용 후 착용 전보다 유의하게 감소하였고 (p<0.05), OSI 값은 유의하게 증가하였다(p<0.05).
결 론: 각막굴절교정렌즈 착용 후 나안시력이 개선되었고 시력의 질을 평가한 MTF cutoff, Strehl ratio 및 OSI 값은 정상 범위 내의 값으로 측정되었으나, 시력의 질은 착용 전보다 저하되고 저대비 객관적시력은 안경렌즈에 의한 교정보다 낮았다.
The oligochaete worms in the genus Nais are common to freshwater habitats. These worms have a highly-developed prostomium and pigmented eye spots. All species have hair chaetae, bifid or single pointed needle chaetae on the dorsal side and ventral chaetae beginning in II with bifid crotchets. Most species of Nais genus live in areas where bottom is covered by sand, gravel or organic matter in aquatic plants. Currently, 26 species of Nais have been recorded worldwide. From the recent faunal studies, 3 species of Nais have been newly reported in Korea. Here, we newly report 6 species of Nais with diagnosis, illustrations and identification keys to Korean Nais species: N. behningi, N. pseudobtusa, N. simplex, N. bretscheri, N. stolci and N. elinguis.
본 연구는 다양한 식재료가 섞여있는 식품으로부터 노로바이러스를 효과적으로 검출하기 위한 시험법 개발에 관한 것이다. 각 식품이 가진 매트릭스가 매우 다르므로 모든 식품에 공통적으로 적용할 수 있는 표준화된 검출법이 현재로서는 없다. 본 연구에서는 발효식품(농후발효유,된장), 절임식품(깻잎장아찌, 단무지)과 생식제품을 대상으로 실험을 진행하였다. PBS, beef extract, 아미노산-NaCl 용액 등을 이용하여 바이러스에 오염된 대상식품들로 부터 바이러스의 탈리율을 비교하였다. 이로부터 다양한 매트릭스가 혼합된 식품들에 적용 가능한 탈리용액을 선별하였다. 식품의약품안전처가 제안하여 현재 국내에서 굴, 야채, 과일 등을 대상으로 바이러스 농축에 널리 사용되고 있는 식중독 바이러스 검출법인 EPCP공정(탈리-PEG 침전-클로르포름 처리-PEG 침전)과 PEG 침전과정을 한번으로 줄인 수정된 ECP공정(탈리-클로르포름 처리-PEG침전)의 효능을 비교해 본 결과 ECP공정은 EPCP공정과 비슷하거나 나은 효율로 바이러스를 농축할 수 있었다. 또 바이러스 농축 후 QIAamp® Viral RNA Mini kit를 이용하여 RNA를 추출할 경우 식품에 존재하는 RT-PCR방해 물질들이 효과적으로 제거되어 추가적인 처리가 더 필요하지 않음을 알 수 있었다. 수정된 공정을 이용하여 무를 추가한 6가지 식품을 대상으로 검출한계를 조사해 본 바 10-25 g 식품으로부터 3.125-12.5 RT-PCR unit까지 검출이 가능하였다. 이는 기존에 보고된 방법들의 검출한계보다 더 우수한 것으로 장차 다양한 식품을 대상으로 효과적인 바이러스 검출이 가능할 것으로 기대된다.
The goniadid species, Glycinde bonhourei Gravier, 1904, is newly reported with the description and illustration from Korean waters. Our Korean materials of G. bonhourei have the following characteristics that are generally known as distinctive features from its congeners: the area II-1 on proboscis possesses unidentate papillae bearing broad base; the area IV on proboscis bears the papillae of duct’s foot-shaped with rounded teeth; the area V on proboscis has straightly conical papillae bearing slightly bifid tip; 4-16 micrognaths are arranged on the dorsal side; all parapodia have one neuropodial presetal lobe; uniramous parapodia are present on 19-26 anterior segments. As a result of the present study, the genus Glycinde is newly reported from Korean waters.
Introduction
This article analyzes retail store openings of luxury fashion brands in international markets. Our aim is to point out the relevance of this market entry strategy as well as to highlight the main destination markets and different trends over the 2004-2013 period.
More precisely, this article analyzes the role of the retail direct channel as a means to manage relationships with consumers in international markets. The choice to develop retail operations in international markets is considered in this article as one of the key strategies implemented by luxury manufacturing companies. However, it seems to have received minor attention in the academic literature dealing with internationalization (Guercini and Runfola, 2014). Consequently, the main aim of this article is to propose empirical evidence to support the widespread use of this strategy by luxury firms, proposing the analysis of an original database built on the retail store operations of a sample of Italian fashion luxury companies over the period 2004-2013.
The retail marketing strategy is a peculiar strategy within the luxury marketing strategies. As stated by Kapferer and Bastien (2012), in fact, through retail store openings (and distribution in general), luxury companies may implement and take advantage from what has been defined by the authors the “watchword of luxury brand management” (p. 233) namely “experience”. In fact, the literature in the field of luxury retailing has pointed out the role of the point of sale from a consumer point of view to experience the value of a company. The discussion on the consumer perspective is increasing in the literature as testified by various contributions aimed at analyzing and discussing how and what kind of experiences could be transferred by the opening of retail stores and in what terms the luxury retail strategy differs from other retail marketing strategies (Dion and Arnould, 2011).
The opening of retail stores from luxury companies has been considered within the stream of research on the internationalization of the company. It has been pointed out that companies with luxury positioning can differentiate their offering with respect to mass market retailers and open retail stores even in culturally distant markets (Hutchinson et al. 2009). These openings, however, are considered more as ways of promoting the brand, rather than a structural international retail development (Moore et al., 2010). In fact, it has been noticed that luxury griffes open retail stores quite exclusively in primary locations (Hutchinson et al. 2009) and that most of the internationalization literature on retail stores openings by luxury firms is referred to the opening of flagship stores (Moore et al., 2010), a specific retail store format that from its nature, is mostly related to brand promotion than to an effective and stable retail development. In fact, retailing as international market entry strategy implies significant investment both in economic and cognitive terms (Mattila el al., 2002; Guercini and Runfola, 2010). The study of retail stores opening as an entry strategy in international markets remains an understudied field of study in the academic literature, as evidenced for example by Ilonen et al. (2011) in their study on the importance of branded retail in manufacturers' international strategy. Moreover, the authors point out that among other things, this remains a topic of interest but not yet analyzed in the case of the fashion industry.
Following this reasoning, our article aims to answer to the subsequent research questions: RQ1 - What is the evolution over time of the distribution investments of luxury fashion manufacturing companies? RQ2 - Is there a difference between emerging markets and advanced markets for luxury retail store openings? RQ3 - What is the role of metropolitan areas and how does this evolve over time?
Methodology and discussion
We investigate these research questions in the case of Italian luxury manufacturing companies. In order to study the expansion of Italian luxury companies, we have exploited the information contained in the database that we have created expressly for the purposes of this research. The database has been compiled by examining any news contained in two specialized and highly recognized national fashion-sector publications - Fashion and Pambianco Week - regarding the opening of retail outlets in foreign countries by Italian luxury firms in the decade 2004-2013. For the purpose of this research we have considered as luxury brands those brands that are members of Altagamma, a association whose members are Italian companies that operate at the highest end of the market, and those brands that are recognized globally and by academics and empirical press as luxury brands, although not being members of Altagamma. The above process has identified 594 sales points opened by 39 Italian brands in 62 countries over the period 2004-2013. The top 10 brands for number of store openings over this period are the followings: Prada (64), Salvatore Ferragamo (59), Miu Miu (51), Ermenegildo Zegna (31), Valentino (29), Armani (26), Versace (26), Gianfranco Ferrè (25), Brioni (22), Etro (22).
Hereafter we try to describe some preliminary findings regarding the three research questions advanced previously.
RQ1 – What is the evolution over time of the distribution investments of luxury fashion manufacturing companies?
Our analysis seems to show an evolution in this growth strategy over the period 2004-2013. In fact, if during the period 2004-2008 our analysis shows the opening of 261 single-brand outlets by the enterprises of our sample, during the period 2009-2013 the number of operations became 333 stores. This seems to highlight how, even in a period of international crisis, the retail strategy for luxury companies remained fundamental for growing abroad. The year 2008 is the year with the maximum number of stores opened by our companies (95 stores, roughly 16% of the total 594 stores opened), while the year 2004 is the one with the minimum number of stores opened, only 35 stores (roughly 6%). Moreover, each year from 2009-2011 accounts for over 70 stores.
RQ2- Is there a difference between emerging markets and advanced markets for luxury retail store openings?
In order to distinguish between “mature”, developed countries and “emerging” ones, we considered the first 24 countries that joined firstly the OCSE as “mature”, while all the remaining countries have been considered “emerging”. Our analysis reveals during the period analyzed a growing incidence by emerging markets compared to mature markets, given that emerging markets account for 60.9% of the openings. Moreover, in each year analyzed emerging markets overcome advanced markets for number of stores opened. However, traditional mature markets for Italian luxury (such as USA or Japan) as well as new emerging markets (such as China and Russia) are within the top destinations all over the period. If we consider only the first three markets for number of retail operations we may note some differences between the two sub-periods. In fact, during the period 2004-2008 the first three markets listed for decreasing number of operations were the USA (45 retail stores opened, 17,2% of the total number of stores), China (29 stores, 11,1% of the total) and India (19 stores, 7,3% of the total). During the period 2009-2013, China increased the number of operations, becoming the leading market with 74 stores, representing 22,2% over the total, followed by the USA (46 stores, 13,2% of the total) and United Arab Emirates (15 stores, 4,5%). The rising of China in the second period, is associated with an increasing importance of other emerging markets such as Brazil and South Korea, that in the previous period were not within the top international destinations. We should however stress that other mature markets, such as France and Japan still have key roles for Italian luxury companies.
RQ3 – What is the role of metropolitan areas and how does this evolve over time?
Our analysis shows that the major cities world-wide are present in our database. In total the companies in our sample have opened stores in 163 cities. Over the period 2004-2013 the top 10 cities listed for decreasing number of stores are the following: Shanghai (30), Hong Kong (28), New York (25), Moscow (24), Tokyo (22), Paris (21), Dubai (20), London (20), Los Angeles (20), Beijing (20). However, as evidenced by the data, while in the period 2004-2008, the total number of cities targeted by the companies were 83, in the following period 2009-2013 the number cities targeted became 127. This data seems to highlight how, over time, the presence of luxury firms is not only concentrated in the top cities around the world neither only in luxury streets, but affects a larger number of cities and locations. Take for example the case of the new rising Chinese cities of the II, III and IV tiers.
To conclude, our research points out how retail strategy implemented by luxury manufacturing companies is one of the driving strategy for relating the company with consumers in international markets. This strategy seems to represent a relevant and widespread used strategy to enter in foreign market and to develop the brand further.
Some considerations are due on the limitations inherent in the present study, which can also furnish some useful indications as to future work. The empirical evidence reported here is based on secondary research in market publications. Aside from collecting further, more up-to-date information, future research should be addressed to performing a number of enterprise case studies in order to acquire a better understanding of the phenomena at play through contacts with luxury enterprise managers with whom to share the main aspects involved in establishing sales networks in foreign countries. Moreover, the considerations advanced are based on empirical evidence drawn solely from study of the Italian luxury fashion industry. In this sense, future research should aim to check if any differences exist in retail store openings between the Italian fashion system and the luxury fashion industries of other economically mature nations (e.g., France, the UK, Japan or the USA).
Although, our empirical analysis has some limitations, it seems to confirm that the retail market strategy is a key strategy to relate with consumers in international markets and to let them “experience” the brand. For manufacturing companies in the luxury field this strategy should not be considered only in terms of promotion, as typically associated with the opening of flagship stores abroad. Rather, it represents an effective retail strategy with important implications from a managerial point of view. Considering this latter point, future research should be directed towards the study of the different strategic behaviors aiming at pointing out different strategic groups within our companies, for example in terms of company size or destination markets. In general terms, future research should be directed towards the study of the link between retail stores openings and customer experience in international markets. This issue has a particular relevance in the case of the Italian fashion industry, where understanding the retail strategy of luxury companies may contribute to recognize potential bandwagon effects of other companies in this sector, such as small and medium sized companies with other positioning.
The activation of glial cells in the spinal cord has been contribute to the initiation and maintenance of pain facilitation induced by peripheral inflammation and nerve injury. The present study investigated effects of botulinum toxin type A (BoNT-A), injected subcutaneously or intracisternally, on the expression of microglia and astrocytes in rats. Complete Freund’s Adjuvant (CFA)-induced inflammation was employed as an orofacial chronic inflammatory pain model. A subcutaneous injection of 40 μL CFA into the vibrissa pad was performed under 3% isoflurane anesthesia in SD rats. Immunohistochemical analysis for changes in Iba1 (a microglia marker) and GFAP (an astrocyte marker), were performed 5 days after CFA injection. Subcutaneous injection of CFA produced increases in Iba1 and GFAP expression, in the ipsilateral superficial lamia I and II in the medullary dorsal horn of rats. Subcutaneous treatment with BoNT-A attenuated the up-regulation of Iba1 and GFAP expressions induced by CFA injection. Moreover, intracisternal injection of BoNT-A also attenuated the up-regulated Iba1 and GFAP expressions. These results suggest that the anti-nociceptive action of BoNT-A is mediated by modulation activation of glial cells, including microglia and astrocyte.
본 연구는 Cymbidium 원종 및 주요 품종을 대상으로 RAPD 분석을 이용하여 이들의 유전적 근연관계를 비교하고, 또한 유전적 구별성과 균일성을 확인하여 이들 생화학적 표지를 품종식별의 지표로 활용함으로써 교배모본을 선정할 때 품종의 기초자료를 얻기 위하여 수행하였다. 심비디움의 PCR 반응조건 구명하고자 각각의 반응액 조건을 알아본 결과 PCR tube(0.5mL)에 10ng template DNA, 100ng primer, 200μM dNTP mixture, 1unit Taq DNA polymerase, 1.5mM MgCl2, 10mM Tris-HCl을 첨가하여 25μL로 조정하여 최적 반응액을 만들었다. PCR 반응 조건은 94oC에서 5분간 예비 변성시키고, 94oC에서 3분 변성, 37oC에서 1분간 primer 접촉 및 72oC에서 5분간 증폭시키는 과정을 45회 반복했을 때가 가장 효과적이었다. 선발된 10개의 primer로부터 87개의 밴드로부터 심비디움 원종 및 품종 30종의 군집분석을 한 결과, 유사도 0.647을 기준으로 2개의 군집으로 분류할 수 있었고, I 집단은 유사도값 0.660을 기준으로 3 소군집, II집단은 유사도값 0.737을 기준으로 3 소군집이 속하였다. II집단 C. ‘Place court’ 와 C. Pure Destiny ‘Ultimate’는 유사도가 0.920으로 높게 나타났다. 중형종인 C. ‘Juulyang’과 C. ‘Tropical Yellow’도 0.908의 높은 유사도 지수를 보여 근연관계가 가깝게 나타났는데 화색은 초록색과 노란색이며 설판의 색과 화형이 비슷한 특징을 보였다. 본 연구를 통해 심비디움 원종 및 품종 30종간의 유전적 근연관계를 밝힘으로써 이후 심비디움 육종 및 유전연구에 유용한 기초자료가 될 수 있을 것으로 생각된다.
Ultra High Performance Fiber Reinforced Concrete (UHPFRC) has a outstanding tensile hardening behaviour after a crack develops, which gives ductility to structures. Existing shear strength model for fiber reinforced concrete is entirely based on crack opening behavior(mode I) which comes from flexural-shear failure, not considering shear-slip behavior(mode II). To find out the mode I and mode II behavior on a crack in UHPFRC simultaneously, maximum shear strength of cracked UHPFRC is investigated from twenty-four push-off test results. The shear stress on a crack is derived as variable of initial crack width and fiber volume ratio. Test results show that shear slippage is proportional to crack opening, which leads to relationship between shear transfer strength and crack width. Based on the test results a hypothesis is proposed for the physical mechanics of shear transfer in UHPFRC by tensile hardening behavior in stead of aggregate interlocking in reinforced concrete. Shear transfer strength based on tensile hardening behavior in UHPFRC is suggested and this suggestion was verified by comparing direct tensile test results and push-off test results.
The purpose of this study is to investigate the behavior characteristics of new hollow reinforced concrete (RC) bridge pier sections with triangular reinforcement details and to provide the details and reference data. Among the numerous parameters, this study concentrates on the shape of the section, the reinforcement details and the spacing of the transverse reinforcement. Additional eight column section specimens were tested under quasi-static monotonic loading. In this study, the computer program, named RCAHEST (Reinforced Concrete Analysis in Higher Evaluation System Technology), was used. A innovative confining effect model was adopted for new hollow bridge pier sections. This study documents the testing of new hollow RC bridge pier sections with triangular reinforcement details and presents conclusions based on the experimental and analytical findings.
짠맛 증진 물질을 탐색하기 위하여 국내에서 시판 중인 간장, 액젓 및 어간장의 품질특성을 분석한 결과, 모든 제 품이 기준·규격에 적합하였으나 까나리액젓의 경우에 식 염의 농도가 기준규격을 약 3% 정도 초과하는 것으로 조 사되었고 ICP-AES를 사용하여 측정한 나트륨 함량으로부 터 환산된 식염 농도는 약 1% 이상 더 높게 나타났다. 풍 미 및 짠맛에 기여하는 성분인 저분자 펩타이드 및 아미노 산이 존재할 것으로 판단되는 수용성 고형분 및 총질소 함 량은 액젓류가 간장류에 비하여 유의적(p<0.05)으로 높게 나타났으며, 유리아미노산 함량을 탐색한 결과, 짠맛과 우 마미를 나타내는 glutamic acid, lysine 및 arginine의 함량 은 멸치어간장 > 저염간장 > 멸치액젓 > 새우육젓 > 양조 간장 > 복합어간장 > 전통간장 > 까나리액젓 > 전통청장 의 순서로 분석되었고 쓴맛에 관여하는 성분은 저염간장 > 멸치어간장 > 멸치액젓 > 새우육젓 > 양조간장 > 복합 어간장 > 전통간장 > 까나리액젓 > 전통청장의 순서로 분 석되었다. 뿐만 아니라 가수분해도 및 질소분포를 분석한 결과 멸치어간장의 경우에 가수분해도가 가장 높았고, 어 간장류에 존재하는 짠맛 증진 물질로 알려진 arginyl dipeptides와 같은 성분이 존재할 것으로 예측되는 아미노 산성 질소와 lowry N의 분율도 멸치어간장이 가장 높았다. 이러한 사실은 arginyl dipeptides가 존재할 것으로 판단되 는 GPC chromatogram의 분획 II가 멸치어간장에 가장 많 이 존재하는 것을 확인할 수 있었다. 짠맛강도 평가결과는 국내 저염 간장을 가장 짜게 느꼈으며, 전통간장 > 창녕 멸치어간장 > 전통청장 > 멸치액젓 > 새우젓 > 까나리 액 젓 > 제주 어간장 > 양조간장 > 소금물 순으로 짠맛을 강 한 것으로 나타나 장기간 숙성이 짠맛을 증진하는데 효과 가 있다고 판단된다. 시판 멸치어간장으로부터 무염의 짠 맛 증진 물질의 분리하기 위하여 MAR을 이용한 흡착 및 탈착 시험으로 탈염처리를 한 결과, 무염의 짠맛 증진 물 질을 분리할 수 있는 가능성은 확인하였으나 짠맛 증진 물 질의 손실이 예상되어 MAR을 이용한 공정은 짠맛증진 물 질의 분리에는 부적합하지만, 멸치어간장 제조방법에서 고 염처리, 장기간 숙성 및 쓴맛 등의 문제를 개선하면 짠맛 증진 물질을 생산할 수 있는 산업적 제조공정을 확립할 수 있을 것으로 판단된다.