간행물
Development & Reproduction KCI 등재 발생과 생식
- 발행기관 한국발생생물학회
- 자료유형 학술지
- 간기 계간
- ISSN 2465-9525 (Print)2465-9541 (Online)
- 수록기간 1997 ~ 2018
- 주제분류 자연과학 > 생물학 자연과학 분류의 다른 간행물
- 십진분류KDC 472DDC 570
권호리스트/논문검색
Vol. 19 No. 3 (2015년 9월) 6건
Articles
1.
2015.09
서비스 종료(열람 제한)
Yun-Gwi Park, Seung-Eun Lee, Eun-Young Kim, Hyuk Hyun, Min-Young Shin, Yeo-Jin Son, Su-Young Kim, Se-Pill Park
The suitable feeder cell layer is important for culture of embryonic stem (ES) cells. In this study, we
investigated the effect of two kinds of the feeder cell, MEF cells and STO cells, layer to mouse ES (mES) cell culture for
maintenance of stemness. We compare the colony formations, alkaline phosphatase (AP) activities, expression of pluripotency
marker genes and proteins of D3 cell colonies cultured on MEF feeder cell layer (D3/MEF) or STO cell layers (D3/STO)
compared to feeder free condition (D3/–) as a control group. Although there were no differences to colony formations and AP
activities, interestingly, the transcripts level of pluripotency marker genes, Pou5f1 and Nanog were highly expressed in D3/MEF (79 and 93) than D3/STO (61and 77) or D3/– (65 and 81). Also, pluripotency marker proteins, NANOG and SOX-2, were more synthesized in D3/MEF (72.8±7.69 and 81.2±3.56) than D3/STO (32.0±4.30 and 56.0±4.90) or D3/– (55.0±4.64 and 62.0±6.20). These results suggest that MEF feeder cell layer is more suitable to mES cell culture. Key words : Mouse embryonic stem cell, Feeder cell, Pluripotency marker, MEF feeder cell
2.
2015.09
서비스 종료(열람 제한)
The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte,
retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically.
Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer
of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height
of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.
3.
2015.09
서비스 종료(열람 제한)
Heat shock protein (HSP) 70, the highly conserved stress protein families, plays important roles in protecting
cells against heat and other stresses in most animal species. In the present study, we identified and characterized four Hsp70
(RuHSP4, RuHSC70, RuHSP12A, RuGRP78) family proteins based on the expressed sequence tag (EST) analysis of the
Korean rose bitterling R. uyekii cDNA library. The deduced RuHSP70 family has high amino acid identities of 72-99% with
those of other species. Phylogenetic analysis revealed that RuHsp70 family clustered with fish groups (HSP4, HSC70,
HSP12A, GRP78) proteins. Quantitative RT-PCR analysis showed the specific expression patterns of RuHsp70 family members in the early developmental stages and several tissues in Korean rose bitterling. The expression of 4 groups of Hsp70 family was detected in all tested tissue. Particularly, Hsp70 family of Korean rose bitterling is highly expressed in hepatopancreas and sexual gonad (testis and ovary). The expression of Hsp70 family was differentially regulated in accordance with early development stage of Rhodeus uyekii.
4.
2015.09
서비스 종료(열람 제한)
Diphlorethohydroxycarmalol (DPHC) is a known to modulate the expression of extracellular matrix (ECM)
components in 3T3-L1. However, the possible role of DPHC in integument stability during obesity induction is not clear yet.
We evaluated the effects of DPHC on collagen or elastic fiber quantity in integument during obesity induction with high-fat
diet. The dorsal back integument sections were stained with hematoxylin–eosin, Masson trichrome, and Verhoff-Van Gieson.
The intensities of collagen fibers and elastin fibers were analyzed with ImageJ. The number of fibroblasts was counted at
×1,000 fields. The number of fibroblast was increased by obesity induction, but DPHC suppressed it in a concentrationdependent
manner both in lean and obese mice. On the other hand, the intensities of collagen fibers were increased by DPHC
treatment in obese mice groups but not in lean mice groups. The intensities of collagen fibers of obese mice were lower than
that of the lean mice in 0% group. However, the number became similar between lean and obese mice by the treatment of
DPHC. The intensity of elastic fibers was increased in the lean mice with the concentration of DPHC. In the obese mice group, there were increasing patterns but only significant at 10% DPHC group. The intensity of elastic fibers of obese mice was higher than lean mice in 0%, 1%, and 10% groups. Histologically epithelial cells and follicle cells which were diffused nuclear staining forms were increased by DPHC treatment. The results suggest that the activity of integument cells during obesity induction can be modulated by DPHC.
5.
2015.09
서비스 종료(열람 제한)
For the 2 years of farming, at the indoor circulating aquaculture system, four kinds of skeletal deformities were found among 60 Far Eastern catfish, Silurus asotus. Deformities saw jawbone’s luxation, abnormality of upper lip and malocclusion. Spinal deformity was most fatal deformities with low weight and small length. Jawbone’s luxation had 1 maxilla and 2 mandibles. Abnormality of upper lip had just lip was back over. Malocclusion’s left maxilla and right maxilla were not balanced. This experiment was any deformities in this species through the deformity can grasp how it affects.
Short communication
6.
2015.09
서비스 종료(열람 제한)
Genomic DNAs were extracted from the muscle of twenty-one specimens of three eel species collected in Anguilla japonica (AJ), Muraenesox cinereus (MC) and Conger myriaster (CM) from the Yellow Sea, respectively. In the present study, 7 oligonucleotides primers generated 191 specific loci in the AJ species, 226 in the (MC) species and 181 in the CM species, respectively. The primer BION-02 generated the most loci (a total of 83), with an average of 11.86 in the AJ species. The specific loci generated by oligonucleotides primers exhibited inter-individual-specific characteristics, thus revealing DNA polymorphisms. With regard to average bandsharing value (BS) results, individuals from Conger myriaster species (0.808) exhibited higher bandsharing values than did individuals from Muraenesox cinereus species (0.729) (P<0.05). The longest genetic distance (0.430) displaying significant molecular difference was also between individual no. 01 within Anguilla japonica eel species and individual no. 04 within Anguilla japonica species. In this study, the dendrogram resulted from reliable seven oligonucleotides primers, indicating three genetic clusters composed of group I (ANGUILLA 01~ANGUILLA 07), group II (MURAENESOX 08~MURAENESOX 14) and group III (CONGER 15~CONGER 21). The existence of species differentiation and DNA polymorphisms among three eel species were detected by PCR analysis. As mentioned above, a dendrogram revealed close relationships between individual identities within three eel species. High levels of a significant genetic distance among three eel species showed this PCR approach is one of the most suitable tools for individuals and/or species biological DNA studies.
