Centella asiatica (C. asiatica) has been widely used in food, cosmetics, and pharmaceutical industry as a functional material. In a previous study, we have investigated not only pharmacological effects such as antioxidative and anti-inflammatory effects, but also analyzed various functional ingredients. In this study, triterpenoids were analyzed using HPLC-DAD to determine marker compounds among functional ingredients. When triterpenoids were analyzed, asiaticoside from C. asiatica was determined as an optimal marker compound. Next, specificity, linearity, limited of detection (LOD), limited of quantification (LOQ), precision, accuracy, and range were evaluated using HPLC-DAD to determine asiaticoside contents in C. asiatica juice and extracts. The specificity was elucidated by chromatogram and retention time using an established analytical method. The coefficient of correlation obtained was 0.9996. LOD was 4.99 μg/mL and LOQ was 15.12 μg/mL. Intra- and inter-day precision of asiaticoside were determined to be 0.48~1.68% and 0.08~1.09%, respectively. Furthermore, the recovery rate of asiaticoside was 98.88% and the analytical range of Field-70E was determined to be 0.625~10 mg/mL. As a results of evaluating ABTS, DPPH, and FRAP antioxidative effect, Field-70E showed potent antioxidant activities. Results of this study could be used as basic data for quality standardization of C. astiatica juice and extracts.

PURPOSES : For most local governments, including that of Gangwon-do, the establishment of an organized pavement management system is insufficient, resulting in problems such as inefficient distribution and use of maintenance budgets for deteriorated road pavements. In this study, we aimed to contribute to the establishment of a more reasonable road maintenance strategy by developing a model for predicting the annual international roughness index (IRI) change for national highway asphalt pavements in Gangwon-do based on big data analysis. METHODS : Data on independent and dependent variables used for model development were collected. The collected data were subjected to exploratory data analysis (EDA) and data preprocessing. Independent variable candidates were selected to reduce multicollinearity through correlation analysis and specific conditions. A final model was selected, and sensitivity analysis was performed. RESULTS : The final model that predicts annual IRI change uses independent variables such as annual temperature range, minimum temperature, freeze-thaw days, IRI, surface distress (SD), and freezing days. The sensitivity analysis confirmed that the annual IRI change was affected in the order of annual temperature range, minimum temperature, freeze-thaw days, IRI, SD, and freezing days. CONCLUSIONS : Road maintenance can be performed rationally by predicting future pavement conditions using the model developed in this study. The accuracy of the prediction model can be improved if additional data, such as material properties and pavement thickness, are obtained in future studies.

To investigate the anti-inflammatory activity of submerged culture using Cordyceps militaris mycelium, culture-including mycelia was extracted and lyophilized into postbiotics (hot-water extract; CM-HW). HW was fractionated into crude polysaccharide (CM-CP) by ethanol precipitation, and CM-CP was further dialyzed into CM-DCP by dialysis with running water using 12~14 kDa dialysis tube. When the cytotoxicity of subfractions against cells was assessed, no subfraction had a cytotoxic impact that was substantially different from the control groups. In an inflammatory model using LPS-stimulated RAW 264.7 cells, CM-DCP significantly decreased IL-6 and MCP-1 production levels compared to the LPS-control group. CM-DCP also inhibited IL-6 and IL-8 secretion in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. In the meanwhile, the neutral sugar content and mannose ratio of anti-inflammatory CM-DCP were higher than the other fractions, and CM-DCP contained β-1,3/1,6-glucan of 216.1 mg/g. High pressure size exclusion chromatography revealed that CM-DCP contained molecules with a molecular weight range of 5.6 to 144.0 kDa. In conclusion, postbiotics of C. militaris mycelium significantly promoted anti-inflammatory activity, suggesting that neutral polysaccharides including Glc and Man contribute to the anti-inflammation in RAW 264.7 or HaCaT cells.

After liquid culture of Phellinus baumii (P. baumii) mycelium (LPBM) was prepared, LPBM was fractionated into A∼E fraction (A; hot-water extract of liquid culture including mycelia, B; crude polysaccharide of A, C; hot-water extract of mycelia, D; crude polysaccharide of C, and E; crude polysaccharide of culture broth) to evaluate for possibility as functional materials with immunostimulatory activity. In macrophage stimulatory activity, E fraction as postbiotics significantly increased secretion of NO and IL-12 from RAW 264.7 cells. Next, when the splenocytes of C3H/HeN mice were primary cultured, E fraction showed significantly mitogenic activity with enhancing mitogen-related cytokines (IFN-γ and TNF-α) production from splenocyte. E fraction also potently stimulated GM-CSF production from Peyer’s patch cells as well as Peyer’s patch-mediated bone marrow cell proliferation. In addition, the immunostimularoy E fraction contained neutral sugar (73.8%), uronic acid (10.6%), protein (7.8%), and polyphenol (7.5%), and mainly consisted of glucose (39.1%), galactose (21.7%), mannose (11.1%), galacturonic acid (9.9%), and arabinose (8.9%) as component sugars. In conclusion, it was demonstrated that postbiotics including exopolysaccharide fractionated from liquid culture of the P. baumii mycelium could enhanced immunostimulatory activity.

본 논문은 장아이링(張愛玲)의 소설에서 공통적으로 느껴지는 분위기, 즉 귀기(鬼 氣)를 통해 그의 작품 세계를 분석한다. 장아이링은 자신의 소설에 귀신을 등장시키 지 않으면서, 스산하고 무서운 분위기를 만들어냈다. 이것은 그의 소설, 특히 단편집 전기(傳奇)에 수록된 소설 전반에 나타나는 특징이다. 신전기(新傳奇)의 표지에 는 ‘귀신’이 직접 등장하기도 한다. 이 ‘귀신’은 이목구비가 없고, 신체 비례가 비현실 적이다. 그는 시대를 알 수 없는 옷을 입은 채 방 안-만청(晚晴) 시대의 풍경을 들여 다본다. 장아이링은 관련 글에서 이 ‘귀신’은 ‘현대인’이며, 독자들이 이를 통해 불안 을 느끼기를 바란다고 쓰기도 했다. 장아이링 소설을 관통하는 귀기는 이 ‘현대인’이 느끼는 불안이다. 그들은 ‘현대인’이라고 불리지만 과거로부터 분리되지 못한다. 대부 분 여성인 이들은 자신의 자리를 찾기 위해 노력하지만 계속 방황하고, 미래는 희망 적이지 않다. 장아이링은 ‘귀신’과 ‘귀기’라는 전기(傳奇)적 요소를 통해 독자에게 이 러한 현실을 환기시킨다.

Purpose: This was a qualitative study using a phenomenological research method. It was conducted to investigate the essential meaning of nursing students’ experiences participating in simulation education with standardized patients. Methods: This study’s research participants included 16 senior employees in the nursing department of two universities. They had experiences participating in simulation education with standardized patients. This study was conducted from June 1 to December 31, 2021, and the data were analyzed using Colaizzi’s (1978) phenomenological method. Results: After analyzing the meaning of simulation experiences with the standardized patients, 101 meaning formations were deduced. From this, 13 theme, 4 theme clusters and 2 categories—effective lesson, tasks to solve. Conclusion: Simulation with standardized patients is a recent approach that has been actively applied in simulation-based learning in nursing colleges. This study conducted qualitative research on students’ experiences with standardized patients and analyzed essential meaning of the experiences. Therefore, it is recommended that simulation education content with standardized patients is developed. This can be achieved by optimizing the advantages of standardized patients with various clinical cases in other subjects besides psychiatry.

The present study investigated the optimum additive ratio of wheat straw pellet as a substitute for beet pulp during oyster mushroom cultivation. The chemical properties across treatments were pH of 4.8–5.5, total carbon content of 45.9–46.5%, total nitrogen content of 1.5–1.7%, and carbon-to-nitrogen ratio of 27.8–31.0. Mycelial growth was the fastest in a medium containing 20% wheat straw pellet (at 88.2 mm in ‘Heuktari’ and 70.3–79.6 mm in ‘Suhan-1Ho’); however, there were no significant differences in mycelial density among the treatments. The characteristics of fruiting bodies by variety were as follows. In ‘Heuktari’, the valid stipe number per bottle (1,100 cc) was 22.5 and yield was 177.1 g/1,100 cc in the mixed medium containing 10% wheat straw pellet, with the values being comparable to those of controls. In ‘Suhan-1Ho’, the valid stipe number per bottle (1,100 cc) was 14.0 and yield was 151.2 g/1,100 cc in the mixed medium containing 10% wheat straw pellet, with the values being comparable to those of controls.

PURPOSES : Local governments in Korea, including Incheon city, have introduced the pavement management system (PMS). However, the verification of the repair time and repair section of roads remains difficult owing to the non-existence of a systematic data acquisition system. Therefore, data refinement is performed using various techniques when analyzing statistical data in diverse fields. In this study, clustering is used to analyze PMS data, and correlation analysis is conducted between pavement performance and influencing factors. METHODS : First, the clustering type was selected. The representative clustering types include K-means, mean shift, and density-based spatial clustering of applications with noise (DBSCAN). In this study, data purification was performed using DBSCAN for clustering. Because of the difficulty in determining a threshold for high-dimensional data, multiple clustering, which is a type of DBSCAN, was applied, and the number of clustering was set up to two. Clustering for the surface distress (SD), rut depth (RD), and international roughness index (IRI) was performed twice using the number of frost days, the highest temperature, and the average temperature, respectively. RESULTS : The clustering result shows that the correlation between the SD and number of frost days improved significantly. The correlation between the maximum temperature factor and precipitation factor, which does not indicate multicollinearity, improved. Meanwhile, the correlation between the RD and highest temperature improved significantly. The correlation between the minimum temperature factor and precipitation factor, which does not exhibit multicollinearity, improved considerably. The correlation between the IRI and average temperature improved as well. The correlation between the low- and high-temperature precipitation factors, which does not indicate multicollinearity, improved. CONCLUSIONS : The result confirms the possibility of applying clustering to refine PMS data and that the correlation among the pavement performance factors improved. However, when applying clustering to PMS data refinement, the limitations must be identified and addressed. Furthermore, clustering may be applicable to the purification of PMS data using AI.

PURPOSES : The purpose of this study is to confirm the thermal expansion characteristics of concrete mixed with 1% waste glass fine aggregates, which is the amount stipulated for recycled aggregates in the current quality standard. METHODS : The coefficient of thermal expansion was measured by applying AASHTOT 336-10 using a LVDT. The results measured were used as physical properties in a finite element analysis to confirm the change in tensile stress and the displacement of the right angle section of the upper slab of a concrete pavement due to admixture substitution. RESULTS : The thermal expansion coefficients of concrete based on the replacement rate of the admixture when the waste glass fine aggregates are replaced are within the range of the thermal expansion coefficients of concrete specified in the Federal Highway Administration report. As the replacement rate of the admixture increases, the thermal expansion coefficient of concrete decreases. As the thermal expansion coefficient decreases, the slab pavement curling displacement and the tensile stress of the center of the upper slab of concrete decrease. CONCLUSIONS : In the short term, the presence or absence of waste glass fine aggregates does not significantly affect the thermal expansion coefficient of concrete. However, in the long term, waste glass fine aggregates are reactive aggregates that causes ASR, which creates an expandable gel around the aggregates and results in concrete expansion. Therefore, the relationship between ASR and the thermal expansion coefficient must be analyzed in future studies.

PURPOSES : In this study, surface distress (SD), rutting depth (RD), and international roughness index (IRI) prediction models are developed based on the zones of Incheon and road classes using regression analysis. Regression analysis is conducted based on a correlation analysis between the pavement performance and influencing factors. METHODS : First, Incheon was categorized by zone such as industrial, port, and residential areas, and the roads were categorized into major and sub-major roads. A weather station triangle network for Incheon was developed using the Delaunay triangulation based on the position of the weather station to match the road sections in Incheon and environmental factors. The influencing factors of the road sections were matched Based on the developed triangular network. Meanwhile, based on the matched influencing factors, a model of the current performance of the road pavement in Incheon was developed by performing multiple regression analysis. Sensitivity analysis was conducted using the developed model to determine the influencing factor that affected each performance factor the most significantly. RESULTS : For the SD model, frost days, daily temperature range, rainy days, tropical nights, and minimum temperatures are used as independent variables. Meanwhile, the truck ratio, freeze–thaw days, precipitation days, annual temperature range, and average temperatures are used for the RD model. For the IRI model, the maximum temperature, freeze–thaw days, average temperature, annual precipitation, and wet days are used. Results from the sensitivity analysis show that frost days for the SD model, precipitation days and freeze–thaw days for the RD model, and wet days for the IRI model impose the most significant effects. CONCLUSIONS : We developed a road pavement performance prediction model using multiple regression analysis based on zones in Incheon and road classes. The developed model allows the influencing factors and circumstances to be predicted, thus facilitating road management.

Purpose: This study aimed to identify and understand the experience of entering graduate school in an advanced practice nursing program in the COVID-19 situation. Methods: This qualitative study used content analysis. The data collection using the focus group and in-depth interviews was conducted from February 11 to September 2, 2022. Results: Six themes and 27 subthemes emerged through the qualitative content analysis. The themes included: “Graduate school: The next step”. “Concerns prior to attending grauate school”, “Learning in the COVID-19 era: Differences between expectations and reality”, “Overcoming the challenges of graduate school”, “Education: A stepping stone to becoming a specialist”, “Changes in self and others’ perceptions”. Conclusion: This study confirmed the growth and change of nurses through the experience of entering an advanced practice nursing program in the COVID-19 era.

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), a chronic contagious disease in small ruminants. The prevalence of CLA has been reported to be >50% in Korean black goats. CLA is difficult to control due to a lack of efficient vaccines and treatment methods. Effective disinfection of the farm environment may be an alternative strategy for reducing the spread of C. pseudotuberculosis. The objective of this study was to evaluate the efficacy of commercial disinfectants against CLA. The six commercial disinfectants, largely composed of sodium dichloroisocyanurate, sodium hypochlorite, potassium monopersulfate triple salt, quaternary ammonium, citric acid, and copper sulfate, were tested against five different genotypes of C. pseudotuberculosis isolated from goat farms in Korea. Efficacy tests were performed in accordance with the disinfectant efficacy test guidelines recommended by the Animal and Plant Quarantine Agency of Korea with slight modifications. All disinfectants except for copper sulfate exhibited >99.99% killing efficacy under hard water conditions following 30 min of incubation, which is the recommended standard treatment time according to guidelines. The minimum bactericidal treatment time was evaluated by employing treatments for durations of 1, 5, and 15 min. The most effective compounds under hard water conditions were sodium dichloroisocyanurate, potassium monopersulfate triple salt, and sodium hypochlorite, exhibiting >99.99% killing efficacy after 1 min of treatment. In the aqueous solution forms, citric acid and the quaternary ammonium compound were the most effective, but required at least 5 min to kill >99.99% of the bacteria. The current study characterizes the killing efficacy of six commercial disinfectant active compounds against C. pseudotuberculosis. Thus, this study provides essential information regarding the efficacy of the disinfectants used to control CLA in goat farms.

Mushroom production in Korea is concentrated on five major mushroom types. To create a new source of income for farmers, it is necessary to establish new mushroom production and marketing systems. This study was conducted to evaluate the marketability of and establish a marketing strategy for Pleurotus nebrodensis variety 'Uram'. The evaluation of distributors showed that it was necessary to cultivate mushrooms of uniform shape and size, to compensate for their low storability, and to sell them in small packages. The consumer evaluation showed that the texture of P. nebrodensis had the highest level of satisfaction for quality, but the levels of satisfaction for size and shape were low. In the consumer evaluation, as in the distributor evaluation, improvements in cultivation were found to be necessary, as the storage time is short due to a high moisture content. An evaluation of the market gave the following results regarding the marketing strategy. It was found to be necessary to consider the production of mushrooms of a uniform shape and size and sell them in small packages in the range of 150 to 300 g. The price of the mushrooms should be set using a high-end strategy for high-end sales. The mushrooms should be introduced to local food and eco-friendly stores in the early stages of production. Subsequently, if farmhouse production increases, shipments should be made to wholesale markets through a regular contract. Finally, considering that P. nebrodensis is an unfamiliar mushroom to consumers, it is necessary to promote it by increasing the accessibility of consumers through tasting events and experience groups.

This study was conducted to investigate the optimum supplementation ratio when replacing cotton seed meal with rapeseed meal for cultivating oyster mushrooms. The chemical properties among the treatments were pH 4.5~4.7, total carbon content was 46.3~46.5%, total nitrogen content was 1.6~1.7%, and carbon-nitrogen ratio was 27.0~27.8. These did not differ significantly from the control. Mycelial growth was 85.1 mm (Heuktari), and 72.8 mm (Suhan-1Ho) in medium containing 5% rapeseed meal, with no significant difference in mycelial density between cultivars. Fruiting body characteristics are as follows: In 'Heuktari', the color of the pileus was darker than control with an L value of 32.5, and pileus thickness and diameter were 3.2 mm and 27.6 mm, respectively. Stipe length and diameter were 86.0 mm and 9.1 mm, respectively. Valid stipe number per bottle (1,100 cc) was 25.9, and a yield of 189.7 g/1,100 cc was recorded in mixed medium containing 5% rapeseed meal (higher than control). In mixed medium containing 10% rapeseed meal, the yield was 184.5 g/1,100 cc (comparable to control). In 'Suhan-1Ho', supplemented with 5% rapeseed meal, pileus color was darker than control color, with an L value of 28.8. Pileus thickness was 4.5 mm. Stipe diameter was 12.3 mm (higher than control), and yield was 145.5 g/1,100 cc (comparable to control).