To produce an intestinal immunomodulatory beverage containing Centella asiatica extract (CAE), three types of CAE-added beverage prototypes were prepared, and their immunomodulatory activities and marker compounds were analyzed. As a result of the cytotoxicity assessment, all the beverages did not show significant toxicity compared to the control group. Next, the immunomodulatory activities of the beverage prototype were evaluated using the inflammatory model of IL-1β-induced intestinal epithelial cell line. All the samples significantly reduced the production of IL-6, IL-8, and MCP-1 in a CAE concentration-dependent manner. In addition, CAE-added beverages inhibited NO, IL-6, and IL-12 production in LPS-induced RAW 264.7 cells. When the major triterpenoids, as marker compounds for the production of CAE-added beverages, were analyzed by HPLC-DAD, only asiaticoside was detected beyond the limit of quantification, while madecassoside, madecassic acid, and asiatic acid were not detected. The amounts of asiaticoside in CAE-added beverage prototypes were confirmed in No. 1 (19.39 μg/mL), 2 (19.25 μg/mL), and 3 (19.98 μg/mL). In conclusion, the results of this study suggested that CAE-added beverage prototypes induced immunomodulatory effects in the intestinal inflammatory cell line models and asiaticoside could be used as a marker compound for CAE-added beverage production.

PURPOSES : The aim of this study is to investigate the enhancement of performance and the mix design method for asphalt mixtures utilizing ferronickel slag, an industrial by-product METHODS : To enhance the performance of FNS asphalt, waste tire powder (CR) was incorporated, and the characteristics of FNS asphalt aggregate, along with the impact of CR, were evaluated through the mix design process. RESULTS : CR is found to be suitable with a size of 30 mesh, and the optimal usage amount is determined to be 1±0.1% of the mixture weight, considering dense grade asphalt mixture. Volumetric design considering the swelling characteristics of CR is necessary, and a mixing design with a consistent tendency can be achieved only when an appropriate VMA is secured. CONCLUSIONS : The mix design for FNS-R asphalt mixture requires an increase of approximately 1% in VMA compared to conventional dense-graded asphalt mixtures to accommodate the swelling of CR. Additionally, FNS-R asphalt exhibits improved resistance to rutting comparable to modified asphalt and meets quality standards, including stripping resistance.

PURPOSES : Derive a road pavement design method using Geocells, aim to derive a road pavement design and construction method suitable for the characteristics of the Bangladesh region METHODS : To assess long-term performance during road construction in Southeast Asia using Geocells, field tests and numerical analysis are conducted to verify stability. RESULTS : A total of 12 displacement measurements were conducted during the field tests, confirming an average load of 15.75 kN and an average displacement of 0.542mm. Inverse analysis was performed to obtain the properties of Geocell combined with compacted soil. The numerical analysis results confirmed that the insertion of Geocell provides better stability compared to the case with only compacted soil. CONCLUSIONS : Based on field tests and numerical analysis, a road design plan suitable for the Southeast Asian environment was proposed. A preliminary test section was selected in the Comilla region of Bangladesh, and test construction has been completed. Subsequent evaluations of the structural performance by soil layer in the test construction area will be conducted to develop a Geocell road pavement method, taking into consideration the characteristics of the Bangladesh region.

PURPOSES : This study is aimed to economic analysis of the ferronickel slag pavement method carried out to suggest the necessity of developing ferronickel slag pavement technology. METHODS : A life cycle cost analysis of the application of the Ferronickel Slag pavement method and the cutting + overlay pavement method was performed to compare the economic indicators and greenhouse gas emissions for each pavement method. RESULTS : As a result of the analysis, regardless of the Ferronickel Slag mixing rate, if the common performance of the Ferronickel Slag pavement method is the same or superior to the existing pavement method, it is more economical than the existing pavement method. Furthermore, the lower the maintenance cost of the Ferronickel Slag pavement method, the higher the economic feasibility due to the high Ferronickel Slag mixing rate. Greenhouse gas emissions can be reduced from at least 9% to up to 53% through the application of the Ferronickel Slag pavement method, except for some scenario analysis results. CONCLUSIONS : This study provided that the Ferronickel Slag pavement method was superior to the existing pavement method in terms of economic and environmental aspects. Therefore, it was found that the objective justification of developing road pavement technology using Ferronickel Slag was secured.

Okadaic acid (OA) group toxins, including OA and its analogs, such as dinophysis toxins (DTXs), have been reported to cause diarrheal shellfish poisoning (DSP). These toxins are primarily produced by dinoflagellates and are accumulated in bivalves. Recently, the presence of Dinophysis sp., a causative alga of DSP, has been reported along the coasts of Korea, posing a potential risk of contamination to domestic seafood and exerting an impact on both the production and consumption of marine products. Accordingly, the European Food Safety Authority (EFSA) and the World Health Organization (WHO) have established standards for the permissible levels of OA group toxins in marine products for safety management. Additionally, in line with international initiatives, the domestic inclusion and regulation of DTX2 among the substances falling under the purview of management outlined by the 2022 diarrheal shellfish toxin standard have been implemented. In this study, we reviewed the physicochemical properties of OA group toxins, their various exposure routes (such as acute toxicity, genotoxicity, reproductive and developmental toxicity), and the relative toxicity factors associated with these toxins. We also performed a comparative assessment of the methods employed for toxin analysis across different countries. Furthermore, we aimed to conduct a broad review of human exposure cases and assess the international guideline for risk management of OA group toxins.

한국의 민주화운동인 4·19혁명, 5·18광주민주화운동 등은 법률에 따라 정신 계승과 선양이 이루어지고 있다. 1987년의 6월항쟁은 한국 민주화 운동의 대단원임에도 이에 대한 정신 계승과 선양을 위한 법·제도적인 근거가 없다. 본 연구는 6월항쟁에 대한 정신 계승과 선양에 사례를 검 토하여 그 한계를 극복하고자 한다. 6월항쟁에 대한 정신 계승과 선양은 교육과정, 민주화단체 등을 통해 이루어지고 있다. 첫째, 교육과정에서 6 월항쟁의 정신 계승은 6월항쟁 30주년 계기수업의 방식으로 도입되었다. 초등교육과정은 5학년의 도덕 과목과 6학년의 사회 과목에서 이루어졌 고, 중등교육과정은 역사 과목에서 읽기수업과 이야기수업으로 이루어졌 으며, 고등교육과정에서는 한국사 과목에서 강의방식과 토론방식으로 이 루어졌다. 둘째, 민주화운동단체들의 6월항쟁에 대한 정신 계승은 2020 년부터 6월항쟁 기념일을 포함해 1주일을 기념행사 기간으로 설정하여 전국적으로 기념식, 학술토론회, 음악회, 사진전 등 다양한 행사를 진행 하고 있다. 셋째, 6월항쟁의 선양은 관련 법률의 미제정으로 한계가 있 음에도 활용할 수 있는 선양 시설을 통해 부분적으로 이루어지고 있다. 결국 6월항쟁의 정신 계승과 선양을 위해서는 다른 민주화운동과의 형평 성에 근거하여 6월항쟁을 위한 법률 제정이 시급하다고 본다.

The effects of punching treatment on mycelial culture and fruiting body productivity were investigated in a new Lentinula edodes cultivar, “Jadam”, in sawdust medium for the stable production of oak mushroom. As the punching volume and number increased, the weight loss rate and color difference increased and the L value decreased. After spawn inoculation, the sawdust medium temperature and CO2 concentration reached their highest values at 33 and 19 days of incubation, respectively. The O2 concentration showed the lowest value on the 14th day of incubation, which was the opposite pattern to the CO2 concentration. As the punching volume and the number increased, the medium temperature and O2 concentration increased, and the CO2 concentration decreased. Higher punching volumes and numbers resulted in higher temperatures and lower CO2 concentrations. The best fruiting body yield was 5 × 70 mm - 30 (punching diameter × depth - number), and the total yield after three cycles was 644.7 g.

A new spore-less cultivar Lentinula edodes ‘Daedam’ was bred from monokaryotic strains of ‘LE15401-24’ and ‘LE192118-10’. The optimum temperature for mycelial growth of ‘Daedam’ on potato dextrose agar was 22~25oC. Total cultivation period of the new cultivar, from inoculation to its first harvest, was 134 days, similar to that of the control cultivar ‘Hwadam’. Total yield of ‘Daedam’ was 222g per 3kg substrate, and was lower than that of control cultivar(266.0g). The fruiting body of ‘Daedam’ had a thick and small pileus and a longer stem compare to control cultivar. As a result of a analyzing the productivity of ‘Daedam’ on the different substrate types, the biological efficiency was 26.7% in the 1.2kg cylindrical substrate(CS), which was higher than that of the 3kg rod-type substrate(RS). 'Daedam' had a similar yield compared to 'Hanacham' in first fruiting body production, but the cultivation period was 40 days shorter. Therefore, 'Daedam' can only harvest fruiting bodies once, it is thought that it can be used as spore-less oak mushroom cultivar for short-term cultivation instead of 'Hanacham' in mushroom farms.

This study was conducted to reduce the phenomenon of the biased cultivation of certain mushroom varieties and to develop a competitive variety of Pleurotus nebrodensis. We have collected and tested characteristics of genetic resources from domestic and overseas varieties since 2015. We bred the domestic variety ‘Boram’. The optimal temperature was 26~29oC for mycelial growth and 15~18oC for fruit body growth temperature. This variety was similar to the control variety (Uram) in terms of the number of cultivation days and yield per bottle. The shape of the new cultivar was round, whereas that of the control group was spatula-like. The yield was 181.1 g/bottle, which was statistically similar to that of the control variety. When incubating the parent and control varieties, the replacement line was clear. Moreover, polymerase chain reaction analysis of mycelial DNA resulted in different band patterns between the parent and control varieties, confirming the hybrid species.

본 연구는 가출청소년의 모래놀이치료 단일사례이다. 참여자는 중2 여학생이다. 본 연구는 2023년 1월 1일~ 2023년 4월29일 까지 총 18회기 진행되었다. 상담 초기 참여자는 분노 감정으로 인하여 쉼터 생활에 적응하지 못하고 재가출을 시 도하고 혼란한 상태였다. 가족에 대한 불신감과 내면 깊이 내재 되어 있는 불안감 으로 계속 부적응을 보여왔다. 학교생활에서도 갈등이 심하고 학업성취도 매우 낮 았다. 자신에 대해 매우 부정적인 자아상을 갖고 있어 주변과 다툼이 지속되었다. 연구 결과, 자신의 깊은 내면의 감정들을 모래상자에 표현하면서 분노감정이 조금 씩 소거돠는 것을 보였다. 쉼터에서의 공격성, 분노, 가출 충동성의 문제성향이 구 체적으로 감소되었다. 학교생활에서도 자신의 문제행동을 스스로 알아차리고 주변과 타협하는 능력을 습득해나갔다. 참여자는 가출한 것을 후회하고 가족에게도 미 안함을 보였다. 참여자의 시각도 매우 긍정적으로 변화되었다.

본 연구는 가정폭력에 노출된 아동의 분노조절을 위한 모래놀이치료이며 단일 사례연구이다. 가정폭력 아동의 분노를 깊이 다루는 것이 본 연구의 필요성이다. 본연구의 목적은 가정폭력 아동의 분노를 조절하는 기초자료가 되는 것이다. 본 연구는 2023년 2월1일부터 6월25일까지 연구하였다. 모래놀이기간은 3월 10일부 터 4월 25일까지 주 2회 총 20회기 회기당 50분 진행하였다. 참여자는 가정폭력 으로 인하여 어머니와 쉼터에 거주하고 있는 초등 2(남아)학년이다. 자료분석은 동적 집. 나무. 사람 그림검사(K-HTP), 동적가족화(KFD)를 사전 사후에 실시하였다. 연구결과, 첫째 모래놀이치료를 통해 가정폭력쉼터 아동의 분노행동이 감소되었 다. K-HTP에서는 무기력감, 고립, 철회와 밀착이 사라지고 적극적이고 사회와 상 호작용하는 모습으로 변화하였으며, KFD에서는 가족의 상호작용과 개인별 역할이 회복되고 가족에 대해 긍정적인 인식으로 변화하였다. 둘째, 모래놀이치료 단계별 변화양상을 살펴본 결과에서도 분노행동이 감소하는 것으로 나타났다. 따라서 본 연구는 모래놀이치료가 가정폭력쉼터 아동의 분노를 없애는 데 유용하다는 것을 알수 있었다.

PURPOSES : The objective of this study is to develop the data driven pavement condition index by considering the traffic and climatic characteristics in Incheon city. METHODS : The Incheon pavement condition index (IPCI) was proposed using the weighted sum concept with standardization and coefficient of variation for measured pavement performance data, such as crack rate, rut depth, and International Roughness Index (IRI). A correlation study between the National Highway Pavement Condition Index (NHPCI) and Seoul Pavement Condition Index (SPI) was conducted to validate the accuracy of the IPCI. RESULTS : The equation for determining the IPCI was developed using standardization and the coefficient of variation for the crack rate, rut depth, and IRI collected in the field. It was found from the statistical analysis that the weight factors of the IPCI for the crack rate were twice as high as those for the rut depth and IRI. It was also observed that IPCI had a close correlation with the NHPCI and SPI, albeit with some degree of scattering. This correlation study between the NHPCI and SPI indicates that the existing pavement condition index does not consider the asymmetry of the original measured data. CONCLUSIONS : The proposed pavement condition provides an index value that considers the characteristics of the original raw data measured in the field. The developed pavement condition index is extensively used to determine the timing and method of pavement repair, and to establish pavement maintenance and rehabilitation strategies in Incheon.

본 연구는 등유를 사용하는 농업용 난방기에서 배출되는 가 스를 포집하고, 농업용 난방기의 사용 연식에 따른 이산화탄 소의 배출농도를 파악하고자 수행되었다. 선형 회귀분석의 결과로 농업용 난방기의 연식에 따른 이산화탄소의 배출량은 R2 = 0.84로 y = 26.99x+721.98의 식을 따른다고 나타났다. 농업용 난방기의 사용 연식에 따라 세 그룹으로 분류하여 분 산분석을 수행하였다. 분산분석을 수행한 결과, 분석을 위해 설정한 유의확률 0.05보다 작은 2.1961×10-13으로 나타났으 며 이는 적어도 한 그룹에서 차이가 나타난다는 것을 의미한 다. 본 연구에서는 농업용 난방기의 기본적인 배출농도의 차 이를 분석하고자 기기의 제작사와 상관없이 농업용 난방기의 기기 연식만을 고려하여 배출가스 데이터를 수집하였다. 기 기의 연소 방식에는 제작사에 따라 연소 방식에 차이가 미미 하게 있었을 것으로 판단되며 데이터 변수의 개수가 늘어난다 면, SVR(support vector regression) 기반의 선형회귀 분석 등 을 실시하여 농업용 난방기의 이산화탄소 데이터가 온실가스 발생량 파악에 더욱 활용도가 높아질 것으로 판단된다. 추후 연구에서는 더욱 세분화된 데이터의 수집 방식을 따라 더욱 높은 정확도를 가진 결과값을 도출할 수 있다고 판단된다. 이 처럼 우리나라의 농업 분야에서 용도별 온실가스 발생량을 조 사하기 위하여 고정형 농기계인 농업용 난방기의 이산화탄소 발생량을 정확히 파악하여 온실가스 배출량 조사에 활용할 수 있을 것으로 판단된다.