Since there had been the first human outbreak of novel influenza A/H1N1 in Mexico, it has become pandemic throughout the world. In the Republic of Korea, the first human case was on May, 2009 and National Veterinary Research and Quarantine Service (NVRQS) has started monitoring novel influenza A/H1N1 on domestic swine farms. The first positive case was detected on 14, Dec, 2009 and until now, we have had total 17 positive cases. From the first case, we did epidemiological investigation on introduction and transmission of virus to pigs. We have concluded that virus had been introduced from humans to pigs, possibly from October to November, 2009 in our country. There were direct and indirect factors including the vehicles transporting veterinary medicine, feed, etc. related to transmission of virus from farm to farm. However, breeding pigs seem to be extrinsic to transmitting factors. Upon the epidemiological investigation, the off-limits of visitors to farms and the disinfection is thought to be critical for blocking the introduction and transmission of novel influenza A/H1N1. In addition, collecting and analyzing the genetic informations of influenza virus is important for predicting and handling the new pandemic influenza in the future.

This paper describes an investigation of an outbreak of foot-and-mouth disease (FMD) in Republic of Korea in January 2010. In this paper, we investigated introduction to the index farm from overseas countries, transmission from index farm to other outbreak farms. The introduction factors were divided direct, indirect factors or airborne spread factor. Based on the epidemiological data, clinical information and other data, in these introduction factors, it was likely that outbreak of FMD in index farm was due to international goods or employees from overseas countries (including China). There were other suggested causes in index farm. But it was less likely that outbreak of FMD in index farm have occurred by other causes. The transmission factors from index farm to other farms were also divided direct, indirect factors or airborne spread factor. In these transmission factors it was possible to make assumptions from index farm to other outbreak farms that the FMD virus was transmitted through animal treatment, persons concerned and persons (who were) attended farmers’ assembly.

본 연구에서는 프리폼(free form)을 가지는 공간구조물의 유한요소망을 자동으로 생성하는 기법을 개발하고 그 배경이론과 수치실험 결과 그리고 이용방안에 대하여 기술하였다. 본 연구를 통하여 제시한 유한요소망 생성기법은 공간구조물의 해석을 위해 전통적으로 이용하던 형태별 자동생성기법의 한계를 극복하기 위하여 개발 되었다. 개발된 자동 생성기법은 최근 연속체 쉘 구조물의 형태를 정확히 구현하기 위해 활발히 이용되고 있는 CAGD기법을 도입하였다. 본 연구에서 제시된 자동생성기법은 두 단계의 생성과정을 거치는데 첫 번째가 구조물의 형태를 기하학적인 모델로 표현하는 단계이며 두 번째가 표현된 기하학적인 모델에 이산화된 공간구조물을 생성하는 단계이다. 수치실험을 통하여 본 연구에서 개발된 자동생성기법이 임의의 형상을 가지는 이산화된 공간구조물을 표현하기 용이할 뿐만이 아니라 기존의 자동생성기법을 수정 이용할 수 있는 장점이 있으며 특히 이산화된 공간구조물의 형상최적화에도 효과적으로 이용될 수 있을 것으로 나타났다.

수소투과금속인 tantalum과 세라믹 지지체로 Y2O3-stabilized ZrO2 (YSZ)를 이용하여 cermet 수소분리막을 제조하였다. Ta/YSZ cermet 분리막은 헬륨분위기에서의 예비소결과 고진공 하에서의 본소결을 통해 제조하였으며, 소결 및 밀봉 과정에서 발생하는 불순물은 연마를 통해 제거 가능하였다. 이렇게 제조된 분리막은 tantalum의 연속상이 잘 발달된 치밀구조를 보였다. 수소 해리를 위해 팔라듐 코팅을 한 Ta/YSZ 분리막을 이용하여 200∼350°C의 범위에서 수소투과실험을 수행하였다. 300°C에서 분리막에 균열이 형성되었고 Pd 코팅층은 몇 곳이 박리되었다. XRD 결과는 tantalum이 수소와 반응하여 Ta2H가 생성되는 것을 보여주며, Ta2H에 인한 격자 팽창이 분리막의 결함을 초래하였다.

Cermet 분리막 제조를 위한 혼합 분말은 60 vol.% vanadium과 Y2O3-stabilized ZrO2 (YSZ)를 기계적으로 혼합하여 준비하였다. 혼합 분말을 원판으로 압축한 후 진공 분위기에서 1600°C로 2시간동안 소결하였다. 소결 분리막은 치밀하였고, 브레이징 필러를 이용하여 스테인레스 링에 장착되었다. V/YSZ 분리막의 수소 투과량은 100% 수소를 흘려 200∼ 350°C 범위에서 측정되었다. 350°C, 0.5 bar압력에서 분리막의 양 표면에 균열이 형성되었다. 투과실험 동안에 V/YSZ 분리막의 vanadium은 수소와 반응하여 V2H를 생성하였으며, 이로 인해 분리막이 균열되는 것을 알 수 있었다.

From April 2008 to January 2009, a total 458 raw milk samples were randomly collected from 15 stock raising farms located in northern area of Kyunggi province and cultured for the presence of Enterococci spp. A total 170 enterococcal isolates were recovered from the raw milk samples. Enterococcus faecalis was predominant species recovered (64.7%), followed by E. faecium (18.8%), E. avium (5.9%), E. gallinarum (5.9%) and E. durans (4.7%). Antimicrobial resistance patterns of 170 Enterococci spp. against ampicillin, erythromycin, tetracyclin, chloramphenicol, vancomycin, ciprofloxacin and streptomycin were tested. According to the result, they showed high level resistance to erythromycin and streptomycin (82,9% and 93,5% respectively), moderately resistance to ampicillin, chloramphenicol and tetracyclin (50%, 45.9% and 32%, respectively) but fortunately, vancomycin and ciprofloxacin are still effective against this species.

The topic of this study is the translation of [Byun Jeung Rok vol.V(辨證錄四卷)]. Byun Jeung Rok is a complete book of medicine which was written by Jin Sa tak(陳士鐸) in the early years of the Ching dynasty. Of this, the fourth volume is composed of 12 disease-patterns(病症) and 54 subtopics. Byun Jeung Rok(辨證錄), as a book that completely organized the medical knowhow of the Ming Dynasty, explains disease-patterns(病症) through the unique and outstanding perspective of the individual author. This topic was studied in hopes of helping later students by showing to the world, through its translation, the author's understanding of disease pattern. The organized characteristics of [Byun Jeung Rok vol.V(辨證錄四卷)] is as follows. 1. For each symptom, the state, cause and prescription for the disease was explained in detail. It was written primarily based on experience. Because the explanations are clear, it is easy to understand. 2. The theory and actual application of Yin yang and five phases is amazing. The actual cause and actual treatment was indicated clearly. 3. In relation to physiology and pathology, the relation between the liver and gall bladder in the middle(between the heart and kidney). In relation to treatment, the tonifying method was never excluded.

In this chapter 8, I discuss a running piglet disease(奔豚氣病), its causes, symptoms and prescription. The name of the running piglet(奔豚) disease dates back to old times, and it is not clinically reported these days. The symptoms of the running piglet disease(奔豚氣病) is characterized by unberable pain in the lower abdomen, the chest and the throat, followed by complete disappearance of these symptoms with passage of time. In my view, the causes of the running piglet disease(奔豚氣病) can be divided into three categories: startle and panic, and the excessive fluid(水飮). In this chapter 13, I elaborate on three diseases: a wasting-thirst(消渴), a dribbling urination(小便不利),1)a strangury disease(淋病). I discuss these three diseases in one chapter because they share the same symptoms of thirst and abnormal urination and the symptoms occur mainly in the kidney(腎) and the urinary bladder(膀胱). A wasting-thirst(消渴) has the main symptoms of drinking water and eating food excessively as well as urinating a lot. A disease symptomized by thirst(消渴) is typically divided into upper wasting-thirst(上消), middle wasting-thirst(中消), and lower wasting-thirst(下消) by clinicians. Specifically, I discuss the mechanism of disease of upper wasting-thirst(上消) and lower wasting-thirst(下消), treatment principles of and prescriptions for upper wasting-thirst(上消) and lower wasting-thirst(下消), and pathology of middle wasting-thirst(中消) in this chapter. Based on the view that a dribbling urination(小便不利) is not a disease per se but a label for the symptom of short and insufficient urination, I discuss water amassment syndrome(蓄水證), yang bringtness disease pattern(陽明病), and a dribbling urination(小便不利) that is accompanied by various diseases. A strangury disease(淋病) has the main symptoms of insufficient and trickling urination that is often accompanied by pain perception. In this chapter, I discuss about stone strangury(石淋) in particular. These three diseases merit in-depth discussion since they are clinically observed with high frequency.

Byun Jeung Ok Ham, written by Jin Sa Tak(1627~1707, won gong, penname ju hwa ja) is composed of four books. this book is estimated of being written after A.D 1688 and composed of internal medicine, obstetrics and gynecology, ophthalmology, dental surgery and so on, total 36 parts of clinical medicine. This is the first book of that four studying about definition on eum yang of 31 diseases. 31 subjects follow, attack by wind and cold(傷風傷寒), paralysis(中風), vomiting(吐症), diarrhea(瀉症), malaria(瘧疾), dysentery(痢疾), epilepsy(癲狂), cough(咳嗽), constipation and urinal impossibility(大小便閉), heartache(心痛), abdominal pain(腹痛), headache(頭痛), eye disease(目痛), tonsillitis(雙蛾), tumor(癰瘡), exhaustion(脫症), perspiration(汗症), congestion(痰症), swelling(腫脹), heatstroke(暑症), asthma(喘症), evil influence(中邪), hematemesis(吐血), wet dream(夢遺), pyrosis(呑酸), backache(腰痛), intestinal convulsion(霍亂). childbirth(生産), hard labor(小産), after delivery(産後), sterility(子嗣). this treatise is the first book of four which deals with eum yang(陰陽) translating into korean and studying about medical theories. In every parts, author's unique clinical theory appears affluently and in that periods his study developmented a lot in those days' medical methods. second book is weaknessand strongness,third upper and low, fourth truth and untruth. first book, Jin Sa Tak says studying on eum yang is oriental medical basic theory and on incurable diseases or chronic symptoms, doctor must go back to that eumyang demonstration after can cure patients. second parts are on weakness and strongness demonstration, that weakness is weakness of patients' energy and strongness is prosperous condition of diseases' attack. third parts are on upper and low, that upper is upper parts of human body of painful parts and low is human low parts of pain, that is parts of under waist. fourth parts are truth and untruth, true symptoms and untrue symptoms, that is the real reaction of human condition and the other way. every prescriptions are author's creations. this book provides new viewpoints which surpasses original ancient medical theories. author suggests new opinions about chronic and incurable diseases. prescrisptions were made of following correct theory of gun sin jua sa theory (君臣佐使) and promoted levels of clinical methods. this treatise refers to dong ui bo gam(東醫寶鑑), huang je nei gyung(黃帝內經), sang han lon(傷寒論), ui hak yip mun(醫學入門) and modern physiology and pathology.

본 연구에서는 선형이론을 바탕으로 한 이산계열 대공간구조물의 크기최적화에 따른 후좌굴 거동의 변화에 대하여 조사하고 그 결과를 기술하였다. 먼저 공간구조물의 최적의 부재크기 패턴을 조사하기 위하여 수학적 프로그래밍 기법을 도입하였다. 이때 최소화 해야하는 공간구조물의 전체 부재의 중량을 목적함수로 이용하고 절점에서 발생하는 변위 값과 각 부재에서 발생하는 응력 값을 허용치 이하로 제한하는 제약조건으로 사용하였다. 크기최적화를 통하여 도출된 최적 부재패턴을 가지는 공간구조물의 후좌굴 거동을 통합 비선형해석기법으로 해석하고 그 결과를 분석하였다. 수치해석을 통하여 크기최적화에 따른 공간구조물의 후좌굴 거동의 변화는 매우 큰 것으로 나타났으며 이러한 후좌굴 거동의 변화에 대한 예측과 분석결과가 공간 구조물의 설계에 고려되어야 할 것으로 판단된다. 또한 본 연구에서 제시한 수치해석 결과는 이산계열 대공간구조물의 설계에 기본 데이터로 제시하였다.

Total water pollution load management(TWPLM) seeks to harmonize conservation and development by allowing regional developments to be carried out in an eco-friendly manner and within the scope of achieving the desirable water quality. Water pollutant sources are managed so as to keep the total amount of pollutants in public watersheds within a certain level or total allowance in accordance with target water quality goal. With relation to the basic guideline under the Special Act on the Water Resources Management and Community Support for Watersheds, the load from the land should be determined by the classification of rainy and dried season respectively. Objective loads of abatement applied in the 1st planning period(2004∼2010) are set up standards by low flow condition(Q275) without considering the change of flow, while allowable wasteloads are estimated by the criteria of daily mean precipitation 10 mm, as critical standard for the division of surface runoff load. Even though the standard flow conditions to meet the goal of water quality at the end of unit watershed are low flow, but it might be considered to set up drought flow condition(Q355) for the improvement of water quality. Also the objective loads for abatement to meet the target water quality should be calculated without the effect of non-point sources from the land.

이 논문은 지오데식 돔 구조물의 구조최적화에 대한 연구내용과 관련이론 그리고 수치해석결과를 기술하고 있다. 지오데식 돔의 공간 효율성을 알아보기 위해 돔의 기저인 정이십면체의 외피면적과 내부공간의 비율을 계산하고 건축구조물에서 나타나는 다른 입방체의 값과 정량적으로 비교하였다. 지오데식 돔을 형성하기 위한 절차를 세부적으로 정리하고 이를 프로그래밍하여 설계최적화프로그램 ISADO-OPT에 연동하였다. 본 연구에서는 반구 형태의 지오데식 돔이 집중하중에 효율적으로 저항할 수 있는 최적의 부재크기 패턴을 조사하기 위하여 수학적 프로그래밍 기법을 도입하였다. 이때 최소화해야하는 돔 전체 부재의 중량을 목적함수로 이용하고 하중이 가해지는 절점에서 발생하는 변위 값과 각 부재에서 발생하는 응력 값을 허용치 이하로 제한하는 제약조건으로 사용하였다. 목적함수와 주어진 제약조건을 만족하는 최적 설계변수값을 검색하기 위해 설계변수에 대한 목적함수의 민감도 값을 유한차분법을 이용하여 계산하였다. 본 연구에서 기술한 지오데식돔을 위한 설계최적화 기본이론을 바탕으로 도출한 최적 부재패턴은 향후 돔의 최적설계에 기본 벤치마크테스트결과로 유용하게 사용될 것으로 판단된다.

본 연구는 Lightcycler (Roche)를 이용한 Real-Time PCR(LC-PCR)기법을 통하여 원유시료에서 신속, 정확하게 황색포도상구균을 검출하는 기법을 개발하고자 하였다. coagulase 전구체를 coding하는 113 bp의 coa 유전자의 증폭, melting curve 분석 및 DNA염기서열을 분석하여 황색포도상구균 특유의 유전자 검출하는 기법을 개발하였다. 또한 분리된 균주중 메치실린에 내성을 나타내는 균주를 검출하고자 penicillin-binding protein, PBP2a (mecA)를 coding 하는 209 bp의 mecA 유전자의 증폭, melting curve 분석 및 DNA염기서열을 분석하여 메치실린내성 황색 포도상구균을 real-time PCR 기법으로 검출하는 기술을 개발하였다. 본 실험에 따르면 647개의 원유시료중 6개의 시료에서 황색포도상구균이 검출되었으며 이중 2개의 시료에서 분리된 황생포도상구균이 메치실린내성 황색포도상구균임을 확인하였다. 또한 DNA 검출한계는 10 fg으로 기존 PCR에 비해 매우 감도가 우수한 것을 확인하였다. 또한 3개의 원유시료에서 돼지나 소의 삼출성 피부염의 원인균인 Staphylococcus chromogenes가 분리되었다.