We studied a storage of waste-brown seaweed at room temperature and degradation of alginate in seaweed by microorganism DS-02. The seaweeds, mixed with 5.0 wt% DS-02 and sealed in vinyl package without any other treatment, could be stored longer than 1 year without spoilage at room temperature. During the storage process, the alginate of seaweed was decomposed by enzyme of DS-02 and the molecular weight of alginate decreased to about 1/10 of initial quantity. DS-02 growed as fast as it had maximum weight after 24 hour culture and it's enzyme had a maximum activity of alginate degradation at 40℃. The seaweed sample became particles in DS-02 culture solution and the M.W of alginate decreased to about 1/10 of initial value after 24 hour decomposition. The effect of alginate degradation with DS-02 was similar to that of degradation with 3.0 M HCl solution for 24 hour.

This study was carried out to clarify the effects of antagonistic microorganism, Bacillus sp. JC181 isolated from the greenhouse soil grown cucumber plants on the growth inhibition of plant pathogen, fusarium wilt (Fusarium oxysporum) occurred in cucumber plants in greenhouse. Antagonistic bacterial strains were isolated and were investigated into the antifungal activity of the antagonistic microorganism against fusarium wilt. Screened fourteen bacterial strains which strongly inhibited F. oxysporum were isolated from the greenhouse soil grown cucumber plants, and the best antagonistic bacterial strain designated as JC181, was finally selected. Antagonistic bacterial strain JC181 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. JC181 showed 58.2% of antifungal activity against the plant pathogen growth of F. oxysporum. By the bacterialization of culture broth and heated filtrates of culture broth, Bacterial strain, Bacillus sp. JC181. showed 91.2% and 26.0% of antifungal activity against F. oxysporum, respectively.

저장조건이 딸기 “미녀봉”의 저장 중 품질 및 미생물학적 변화에 미치는 영향을 연구하기 위하여 저장 중 중량감소율, 부패율, pH 및 적정산도, 총균수 및 곰팡이와 효모 수의 변화가 측정되었다. 딸기 시료는 LDPE로 포장하여 4와 2에서 각각 저장하였다. LDPE포장은 대조구에 비해 중량감소율 및 부패율을 감소시켰다. 또한 포장방법은 저장중 미생물학적 변화에 영향을 끼쳤는데 저장온도와 상관없이 대조구와 비교해서 효모와 곰팡이뿐만 아니라 총균수의

This study was conducted to screen the antagonistic bacteria which inhibit the growth of plant pathogen, fusarium wilt(Fusarium oxysporum) occurred in tomato plants in greenhouse. We isolated an effective bacterial strains and investigated into the antifungal activity of the antagonistic microorganism and it"s identification. Ten bacterial strains which strongly inhibited Fusarium oxysporum were isolated from the nature, and the best antagonistic bacterial strain designated as KC175, was selected. The antagonistic strain KC175 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. The Bacillus sp. KC175 showed 58.2% of antifungal activity against the growth of Fusarium oxysporum. By the bacterialization of the culture broth and the heat bacterialization culture filtrate of it, Bacillus sp. KC175 showed 91% and 18% of antifungal activity, respectively.

도체표면에서 분리한 E. coli O157;H7 CDF1, A. sobria CDF3, S. aureus CDF2의 성장을 억제하기 위한 방안을 검토하였다. E. coli O157:H7 CDF1와 A. sobria CDF3는 NaCl 4% 이상 농도에서 성장이 억제된 반면 lactic acid 0.1%는 성장에 영향을 미치지 않았다. S. aureus CDF2은 NaCl 농도 4%에 의해 성장이 뚜렷하게 억제되지 않았으나 lactic acid 0

분만후 젖소의 자궁내 미생물상을 조사하고 미생물로부터 분리한 Lipopolysaccharide를 적용하여 소의 번식효율 증진에 기여하고자 분만후 젖소의 도축장 유래 자궁을 채취하여 혐기적 상태에서 균분리 동정을 실시하였다. 균분리 동정을 위하여, 시료를 1cm1cm로 채취하여 혐기상태에서 거품이 생길 때까지 vortexing한 후 균액 300를 뽑아 혐기배지에 도말하였고 도말한 plate는 혐기chamber에서 24시간 배양하였다. 혐기배지에서 자란 균

This study was conducted to investigate the effect of microbial liquid manure(MLM) on the growth and yield characteristics of Altari radish ( Raphanus sativus L.). MLM 1,000㎎ㆍL⁻¹, MLM 2,000㎎ㆍL⁻¹ and MLM 1,000㎎ㆍL⁻¹+microbial fermentation compost(MFC) 600㎏/10a were treated. MLM was sprayed on leaves at 10, 20, and 40 days after planting. Leaf length and width of radish in all microbial fertilizer treatments were more vigorous than that of control significantly. Number of leaves was great in order of 1,000 ㎎ㆍL⁻¹ MLM+600㎏/10a MFC ＞ 2,000㎎ㆍL⁻¹ MLM ＞ 1,000㎎ㆍL⁻¹ MLM. SPAD reading value was increased with treatments of 2,000㎎ㆍL⁻¹ MLM and 1,000㎎ㆍL⁻¹ MLM+600㎏/10a MFC. Root length and weight of all the microbial fertilizer treatments tended to increase compared with control, and 1,000㎎ㆍL⁻¹ MLM+600㎏/10a MFC treatment was the most effective.

It was investigated the possibility to use charcoal powder as beneficial soil conditioner, which used frequently in environmentally friendly agricultural farming system. For this purpose, the effects of charcoal powder on the growth of red pepper and chemical and microbiological properties of soil were also determined. The application of charcoal powder resulted in no significant differences of pH and EC in the soil compared to those of control. However, small particle size of charcoal powder increased yield of red pepper while large charcoal powder resulted in decrease of root growth of red pepper. Furthermore, the application of charcoal powder resulted in changes of soil microflora relating to plant growth stage. The number of the nitrogen fixing bacteria and fungi increased at the early growth stage, while phosphate releasing fungi in the soil increased at the late stage of growth by charcoal powder application. These beneficial effect of charcoal powder on the soil microbial properties was larger by the use of smaller particle size of charcoal powder. Therefore, it indicated that the small size of charcoal powder might be more influential on the red pepper yield and soil microbial properties may be due to large capacity of nutrients uptake for the plant and microorganisms. Additionally the optimal application amount of charcoal powder for the red pepper could be suggested as much as 300㎏ 10a⁻¹ for the both purposes of improvement of crop yield and retardation of the nutrients accumulation by excess charcoal application.

일반적으로 실제 저장 유통되는 조건 하에서 생선의 품질 변화를 예측하기 위하여 대표적 생선인 고등어, 조기, 꽁치를 대상으로 미생물과 pH의 변화를 측정하였다. 고둥어의 저장조건으로는 실제 유통되고 있는 조건인 실온 얼음상과 에 저장하면서 그 변화를 관찰하였으며 조기와 꽁치는 와 4에 각각 저장하면서 저장온도에 따른 변화를 관찰하였다 고등어의 초기 총균수는 3.2CFU/g 이었으며 실온 얼음상에서는 저장 6일 후 8.4까지 증가하였다. 반면,

대두단백 가수분해 산물의 맛과 향을 개선하기 위해 효소에 의한 가수분해 system을 확립하기 위하여 단백질 가수분해 패턴이 서로 다른 효소로 생산된 단백 분해산물의 가수분해도와 표면 소수도 등을 측정하였다. 이들 분해물의 pattern을 SDS 전기영동으로 조사하였고, 효소반응에 의한 단백질분해물의 관능검사를 실시하였다. 각 균주가 생산한 단백질 분해효소의 pH 변화에 따른 효소의 활성은 No. 16 효소(Bacillu megarerium Bl