난 가공식품인 구운 계란의 포장유통을 목적으로 구운 계란 제조공정 중의 미생물 오염도를 평가하였으며 미생물 살균방법으로 감마선 조사와 고온가압살균법을 비교하여 제품의 보존 안정성 및 유효 유통기한 확보를 위한 살균 기준을설정하였다. PE 포장지에 낱개 포장한 구운 계란의 미생물오염도는 일반세균 > 10 CFU/g, 대장균군 < 10 CFU/g, 곰팡이 포자 > 10 CFU/g 수준이었으며 대부분의 미생물은 제품포장과정에서의 2차 오염에 의하여 오

갈변과 미생물 증식으로 인해 저하되는 양상추의 품질을 향상시키기 위해 양상추를 최소처리하여 이들 품질저하요인을 제어하고자 하였다. 갈변억제방법으로는 다양한 침지수에 따른 화학적인 방법을 사용하였고, 미생물 증식억제방법으로는 염소처리법과 전기분해수 그리고 유기산 처리에 의한 방법을 사용하였다. 그 결과 양상추의 갈변은 색도에 있어서 ascorbic acid 1와 citric acid 1 혼합침지수로 처리하였을 때 초기 L 값이 5 수준에서 유의적으

In recent years, the consumption of livestock products were markedly decreased by awareness of world-widely occurred diseases including mad cow disease, Foot and mouth disease, Hog cholera, and Poultry Influenza virus. The consumers are also wanting to have highly safe food such as organic animal products because of concerning about residual of antibiotics in animal products. However, disease control and impairment of productivity are the major problem in organic animal production. On these points of view, the present study was undertaken to investigate the effects of 1% or 2% of dietary probiotics fortified with various minerals on improvement of egg production and egg quality in old layer feeding low quality feed mainly composed of food waste, sesame meal, and rice bran. After 4 weeks of experimental feeding, the diameter of spreading of egg white was clearly decreased from 11.2cm of control eggs to 10.5m and 10.1m in 1% and 2% treatment group eggs, respectively. The color of egg yolk was 9.3 in control eggs but remarkably increased in treatment groups showing 10.1~10.2. Egg production was 75.8% in control layers but significantly increased to 79.8% of 1% treatment group and 82.9% of 2% treatment group layers. Egg weights (C : 66.3g, 1% : 73.2g, and 2% : 76.7g) and egg shell thickness (C : 0.33㎜, 1% : 0.35㎜ and 2% : 0.36㎜) were also increased by the addition of 1% or 2% of probiotics when compared to those of control group eggs. All together, it has been suggested that dietary addition of probiotics fortified with various minerals can improve the egg quality and egg production in layer"s productivities by the recycling of organic waste resources such as food waste and agricultural by-products.

다양한 기능성을 갖는 생약재 32종을 대상으로 천연보존료 개발의 일환으로 메탄올로 추출한 항균활성 물질을 몇 종의 병원균과 식중독균, 식품과 관련이 있는 세균, 젖산균 및 효모 등 12종의 균주 B. subtilis, M. luteus, Sta aureus, Sta. epidermidis, Strep. mutans, E. coli, Pse. aeruginosa, Sal. typhimurium, V. vulnificus, Lac. brevis, L

We studied a storage of waste-brown seaweed at room temperature and degradation of alginate in seaweed by microorganism DS-02. The seaweeds, mixed with 5.0 wt% DS-02 and sealed in vinyl package without any other treatment, could be stored longer than 1 year without spoilage at room temperature. During the storage process, the alginate of seaweed was decomposed by enzyme of DS-02 and the molecular weight of alginate decreased to about 1/10 of initial quantity. DS-02 growed as fast as it had maximum weight after 24 hour culture and it's enzyme had a maximum activity of alginate degradation at 40℃. The seaweed sample became particles in DS-02 culture solution and the M.W of alginate decreased to about 1/10 of initial value after 24 hour decomposition. The effect of alginate degradation with DS-02 was similar to that of degradation with 3.0 M HCl solution for 24 hour.

This study was carried out to clarify the effects of antagonistic microorganism, Bacillus sp. JC181 isolated from the greenhouse soil grown cucumber plants on the growth inhibition of plant pathogen, fusarium wilt (Fusarium oxysporum) occurred in cucumber plants in greenhouse. Antagonistic bacterial strains were isolated and were investigated into the antifungal activity of the antagonistic microorganism against fusarium wilt. Screened fourteen bacterial strains which strongly inhibited F. oxysporum were isolated from the greenhouse soil grown cucumber plants, and the best antagonistic bacterial strain designated as JC181, was finally selected. Antagonistic bacterial strain JC181 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. JC181 showed 58.2% of antifungal activity against the plant pathogen growth of F. oxysporum. By the bacterialization of culture broth and heated filtrates of culture broth, Bacterial strain, Bacillus sp. JC181. showed 91.2% and 26.0% of antifungal activity against F. oxysporum, respectively.

저장조건이 딸기 “미녀봉”의 저장 중 품질 및 미생물학적 변화에 미치는 영향을 연구하기 위하여 저장 중 중량감소율, 부패율, pH 및 적정산도, 총균수 및 곰팡이와 효모 수의 변화가 측정되었다. 딸기 시료는 LDPE로 포장하여 4와 2에서 각각 저장하였다. LDPE포장은 대조구에 비해 중량감소율 및 부패율을 감소시켰다. 또한 포장방법은 저장중 미생물학적 변화에 영향을 끼쳤는데 저장온도와 상관없이 대조구와 비교해서 효모와 곰팡이뿐만 아니라 총균수의

This study was conducted to screen the antagonistic bacteria which inhibit the growth of plant pathogen, fusarium wilt(Fusarium oxysporum) occurred in tomato plants in greenhouse. We isolated an effective bacterial strains and investigated into the antifungal activity of the antagonistic microorganism and it"s identification. Ten bacterial strains which strongly inhibited Fusarium oxysporum were isolated from the nature, and the best antagonistic bacterial strain designated as KC175, was selected. The antagonistic strain KC175 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. The Bacillus sp. KC175 showed 58.2% of antifungal activity against the growth of Fusarium oxysporum. By the bacterialization of the culture broth and the heat bacterialization culture filtrate of it, Bacillus sp. KC175 showed 91% and 18% of antifungal activity, respectively.

도체표면에서 분리한 E. coli O157;H7 CDF1, A. sobria CDF3, S. aureus CDF2의 성장을 억제하기 위한 방안을 검토하였다. E. coli O157:H7 CDF1와 A. sobria CDF3는 NaCl 4% 이상 농도에서 성장이 억제된 반면 lactic acid 0.1%는 성장에 영향을 미치지 않았다. S. aureus CDF2은 NaCl 농도 4%에 의해 성장이 뚜렷하게 억제되지 않았으나 lactic acid 0

분만후 젖소의 자궁내 미생물상을 조사하고 미생물로부터 분리한 Lipopolysaccharide를 적용하여 소의 번식효율 증진에 기여하고자 분만후 젖소의 도축장 유래 자궁을 채취하여 혐기적 상태에서 균분리 동정을 실시하였다. 균분리 동정을 위하여, 시료를 1cm1cm로 채취하여 혐기상태에서 거품이 생길 때까지 vortexing한 후 균액 300를 뽑아 혐기배지에 도말하였고 도말한 plate는 혐기chamber에서 24시간 배양하였다. 혐기배지에서 자란 균

This study was conducted to investigate the effect of microbial liquid manure(MLM) on the growth and yield characteristics of Altari radish ( Raphanus sativus L.). MLM 1,000㎎ㆍL⁻¹, MLM 2,000㎎ㆍL⁻¹ and MLM 1,000㎎ㆍL⁻¹+microbial fermentation compost(MFC) 600㎏/10a were treated. MLM was sprayed on leaves at 10, 20, and 40 days after planting. Leaf length and width of radish in all microbial fertilizer treatments were more vigorous than that of control significantly. Number of leaves was great in order of 1,000 ㎎ㆍL⁻¹ MLM+600㎏/10a MFC ＞ 2,000㎎ㆍL⁻¹ MLM ＞ 1,000㎎ㆍL⁻¹ MLM. SPAD reading value was increased with treatments of 2,000㎎ㆍL⁻¹ MLM and 1,000㎎ㆍL⁻¹ MLM+600㎏/10a MFC. Root length and weight of all the microbial fertilizer treatments tended to increase compared with control, and 1,000㎎ㆍL⁻¹ MLM+600㎏/10a MFC treatment was the most effective.

It was investigated the possibility to use charcoal powder as beneficial soil conditioner, which used frequently in environmentally friendly agricultural farming system. For this purpose, the effects of charcoal powder on the growth of red pepper and chemical and microbiological properties of soil were also determined. The application of charcoal powder resulted in no significant differences of pH and EC in the soil compared to those of control. However, small particle size of charcoal powder increased yield of red pepper while large charcoal powder resulted in decrease of root growth of red pepper. Furthermore, the application of charcoal powder resulted in changes of soil microflora relating to plant growth stage. The number of the nitrogen fixing bacteria and fungi increased at the early growth stage, while phosphate releasing fungi in the soil increased at the late stage of growth by charcoal powder application. These beneficial effect of charcoal powder on the soil microbial properties was larger by the use of smaller particle size of charcoal powder. Therefore, it indicated that the small size of charcoal powder might be more influential on the red pepper yield and soil microbial properties may be due to large capacity of nutrients uptake for the plant and microorganisms. Additionally the optimal application amount of charcoal powder for the red pepper could be suggested as much as 300㎏ 10a⁻¹ for the both purposes of improvement of crop yield and retardation of the nutrients accumulation by excess charcoal application.

일반적으로 실제 저장 유통되는 조건 하에서 생선의 품질 변화를 예측하기 위하여 대표적 생선인 고등어, 조기, 꽁치를 대상으로 미생물과 pH의 변화를 측정하였다. 고둥어의 저장조건으로는 실제 유통되고 있는 조건인 실온 얼음상과 에 저장하면서 그 변화를 관찰하였으며 조기와 꽁치는 와 4에 각각 저장하면서 저장온도에 따른 변화를 관찰하였다 고등어의 초기 총균수는 3.2CFU/g 이었으며 실온 얼음상에서는 저장 6일 후 8.4까지 증가하였다. 반면,