일부 고주파 음파 처리가 파밤나방(Spodoptera exigua)의 생리변화를 유발시킨다. 이 연구는 초음파( 20 kHz) 처리가 파밤나방 유충 섭식, 용 발육 및 성충 교미행동에 미치는 영향을 분석하였다. 초음파 처리는 5령충의 섭식 활동을 억제시켰다. 특별히 30 kHz 또는 45 kHz 초음파 처리를 받은 유충은 50% 이상의 섭식활동이 감소하였다. 이러한 초음파 처리를 받은 유충은 혈장의 주요 영양물질 함량이 변동되었다. 혈장 단백질은 처리 초음파의 주파수 증가에 따라 감소하였다. 그러나 혈당은 처리 초음파의 주파수 증가에 따라 증가하였다. 지질 함량은 30 kHz 처리까지는 증가하다가 이후 감소하였다. 파밤나방 5령의 혈구, 지방체 및 표피세포의 세 조직은 스트레스 관련 유전자들인 세 종류의 열충격단백질과 apolipophorin III를 발현시켰다. 그러나 초음파를 처리할 경우 일부 스트레스 관련 유전자들의 발현을 크게 억제시켰다. 초음파 처리는 또한 용발육을 억제시켜, 용기간을 연장시키고 성충으로 우화를 현격하게 낮추었다. 끝으로 초음파 처리는 성충의 교미행동을 억제시켜 암컷의 산란력을 뚜렷하게 낮추었다. 이러한 결과는 초음파가 파밤나방의 생리적 스트레스로 작용하고 있다는 것을 제시하고 있다.

This research is a key words analysis on Radio Frequency Identification. Key words were collected from Korean research papers in the electronic library DBpia. 700 papers published from 2001 to 2011 were included. The number of collected key words is 1460. The trend of publishing research papers was increased rapidly from 2005, reached peak at 2009 and decreased after 2010. Majority of key words were related to hardware, information technology and standardization. Selected 128 key words were analyzed and clustered by social network analysis to find a relationship among key words on RFID.

The decreased fertility is frequently thought to be problem of cattle production. However, studies figure out that number of these problems is related to bull factors especially in artificial insemination setting. Therefore, this study was designed to investigate the fertility status of bull by their estimated relative conception rate of cows that were inseminated by frozen semen from Korean proven bulls. Here we use the non-return rate (NRR) to access the bull fertility whereas, the NRR was define as the proportion of bulls that semen were used to inseminate cows and the number of cows that did not return for another service within 60 days. The data from 54,388 artificial inseminations (AI) were analyzed from 88 KPN semen. The NRRs of highest and lowest fertile bull were 83.81 and 51.33%, respectively. And mean NRR was 68.27%. In comparison to previously reported study, our data shows 17.38% higher NRR and the absolute value of difference in 50%>NRR and 50%<NRR group was 22.17 and 10.51, respectively (p< 0.001). In conclusion, the decreased fertility might consider as key aspect in achieving considerable conception of cows in existing integrated farming system at Korea.

목적: 독서능력을 평가하는 도구로 DEM검사를 활용할 수 있는지 확인하였다. 방법: 초등학교4-6학년 246명을 대상으로 독서속도를 직접 측정하는 Visagraph II검사와 DEM검사를 진행한 후, Visagraph II검사의 평균주시횟수, 평균반복횟수, 평균주시시간 및 평균독서속도가 DEM검사의 수직 및 수평보정시간, 오류갯수 및 수평/수직시간비율과 각각 상관성이 있는지 조사하였다. 결과: DEM검사의 수직보정시간은 Visagraph II의 평균주시횟수, 평균주시시간 및 평균독서속도와 각각 유의한 상관성이 있었으며, DEM검사의 수평보정시간은 평균반복 횟수만 유의한 상관성이 있었다. 그러나 DEM검사의 오류갯수 및 수평/수직시간비율은 Visagraph II결과와 유의한 상관성이 없었다. 결론: DEM검사의 중요한 요소인 수평보정시간과 수평/수직시간비율이 Visagraph II의 평균독서속도와 유의한 상관성이 없었으므로, 임상에서 독서능력을 평가하기 위해DEM검사를 활용하는 것은 추가적인 검증이 필요할 것으로 사료된다.

The purpose of this study was to evaluate microbiological contamination of fresh-cut produce salads and raw cabbage toward climate change. Total aerobic bacteria, coliform and Escherichia coli were monitored to get the contamination levels and E. coli O157:H7, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes and Salmonella spp. to detect pathogens with risk of foodborne disease from samples. Collection of 360 samples (180 fresh-cut produce salads and 180 raw cabbage), including 60 samples from each area after setting 3 areas depending on annual temperature and annual rainfall. As a result, total aerobic bacteria and coliform group were different was performed areas in raw cabbage but there was no difference between areas in fresh-cut produce salads. In addition foodborne pathogens including E. coli were not isolated from fresh-cut produce salads.

The bovine fatty acid binding protein 4 and 5 (FABP4 and 5) is a major positional and physiological candidate gene for the bovine marbling and carcass weight. The aim of this study was to evaluate the association between economic traits of Korean cattle (Hanwoo) and genetic variation in fatty acid binding protein 4 and 5 (FABP4 and 5) genes within carcass/meat quality traits and the before/after of fatting in breed Hanwoo. Here, we characterized the nucleotide polymorphism of FABP4 and 5 in 86 cattle. We were detected the variability of three types (GG, AG, and AA) by PCR, and economic traits were analyzed by the mixed regression model implemented in the ASReml program. As the result of statistical and supersonic analysis, FABP4 gene was highly showed significant effect (p<0.006) on marbling score (MS), in contrast FABP5 gene was lowed (p<0.084) on MS before fatting. But, FABP4 gene was highly showed significant effect (p<0.0054) on MS, in contrast FABP5 gene lowest (p<0.0899) on MS in the after of fatting. Compare to supersonic result before fatting in FABP4 gene, it was detected type GG: (p<7.18), AG: (p<8.50), and AA: (p<10.50) (n=50), showed type GG: (p<4.88), AG: (p<2.33), and AA: (p<0.00) after weed out (n=20). Futhermore, it was detected type GG: (p<9.30), AG: (p<7.95), and AA: (p<7.40) (n=50) before fatting in the FABP5 gene. It was shown type GG: (p<2.67), AG: (p<3.50), and AA: (p<5.00) after weed out (n=50). Our results indicate that FABP4 and 5 gene transcription is regulated by the environment of feeding and management, and suggest that feeding and management could be potential key in determining FABP4 and 5 genes transcription for carcass/meat quality traits in breed Hanwoo.

The synovial tissues are a valuable MSCs source for cartilage tissue engineering because these cells are easily obtainable by the intra-articular biopsy during diagnosis. In this study, we isolated and characterized the canine MSCs derived from synovial fluid of female and male donors. Synovial fluid was flushed with saline solution from pre and post-puberty male (cM1-sMSC and cM2-sMSC) and female (cF1-sMSC and cF2-sMSC) dogs, and cells were isolated and cultured in advanced-DMEM (A-DMEM) supplemented with 10% FBS in a humidified 5% atmosphere at . The cells were evaluated for the expression of the early transcriptional factors, such as Oct3/4, Nanog and Sox2 by RT-PCR. The cells were induced under conditions conductive for adipogenic, osteogenic, and chondrogenic lineages, then evaluated by specific staining (Oil red O, von Kossa, and Alcian Blue staining, respectively) and analyzed for lineage specific markers by RT-PCR. All cell types were positive for alkaline phosphatase (AP) activity and early transcriptional factors (Oct3/4 and Sox2) were also positively detected. However, Nanog were not positively detected in all cells. Further, these MSCs were observed to differentiate into mesenchymal lineages, such as adipocytes (Oil red O staining), osteocytes (von Kossa staining), and chondrocytes (Alcian Blue staining) by cell specific staining. Lineage-specific genes (osteocyte; osteonectin and Runx2, adipocytes; PRAR-, FABP and LEP, and chondrocytes; collagen type-2 and Sox9) were also detected in all cells. In this study, we successfully established synovial fluid derived mesenchymal stem cells from female and male dogs, and determined their basic biological properties and differentiation ability. These results suggested that synovial fluid is a valuable stem cell source for cartilage regeneration therapy, and it is easily accessible from osteoarthritic knee.

가상 환경 또는 실세계에서 지능적인 NPC를 위한 연구가 하드웨어나 소프트웨어 분야에서 활발히 진행되고 있다. 하드웨어 분야로는 로봇 시스템이 주를 이루고 있으며, 이는 물리적인 제약점과 노이즈의 문제 해결에 초점을 맞추고 있다는 점에서 단순 소프트웨어 분야와는 차이점이 있다. 그러나 공통적인 목표는 사람과 같은 환경에서 사람과 같이 행동하는 지능형 NPC 기술 개발이다. 본 논문은 최근 기존 연구 중에서 소프트웨어 분야와 아키텍쳐 설계를 통해 지능적인 NPC를 연구한 사례를 중점으로 조사한다. 사람과 같은 행동 결정을 유도하는 NPC에는 신경망, 유전자 알고리즘, 사례기반 추론, 학습과 같은 여러 분야에 연구가 진행되고 있다. 다양한 인공지능 알고리즘 중에서 현재 환경과 자신의 상태를 고려하여 가장 적합한 행동 결정을 유도하는 계획 기법을 기반으로 하는 연구를 조사한다. 이러한 연구는 기존의 실세계의 로봇 시스템, 가상 환경, 가상 시뮬레이션 그리고 게임의 여러 캐릭터 위주의 장르에서도 활용범위를 넓힐 수 있다.

수집한 83개의 뽕나무버섯속 균주의 rDNA의 ITS 영역을 증폭하여 염기서열을 분석한 결과 수집한 균주목록과 종이 다르게 동정된 균주가 52%였으며. 같은 종으로 분류한 균주들 간에도 균사 및 균사체의 배양 특성에 많은 차이를 보였다. 수집 균주간의 유전적인 유연관계 분석 결과 A. tabescens, A. mellea, A. novae-zelandia, A. gallica, A. ostoyae 등으로 분류되었고, A. gallica, A, cepistipes, A, gemina는 매우 가까운 유연관계를 보여 ITS 유전자 수준에서 종을 동정하기는 어려웠다. ASI10104 등 12균주는 A. gallica, A, cepistipes, A, gemina와 매우 가까운 유연관계를 보였으며, ASI10017과 ASI10114는 A. sinapina, ASI10045는 A. borealis, ASI10002와 ASI10025는 A . ostoyae와 같은 그룹으로 분류되었다. 따라서 뽕나무버섯속 균주에 대한 보다 정확한 동정을 위해서는 보다 많은 종류의 유전적인 분석이 필요할 것으로 판단된다.

This study was conducted to evaluate the characteristics of exposure to asbestos for ship repair workers in Korea by a systematic review. The number of articles studying asbestos exposure levels from ship repair workplaces was found to be 4. All asbestos concentration reported as either geometric mean and geometric standard deviation or ranges were transformed to arithmetic mean to estimate exposure level. In addition, weighted arithmetic means(WAMs) were calculated by weighing of the different number of samples. The WAM concentrations were 2.746 f/cc during asbestos dismantling work, 0.034 f/cc before asbestos dismantling work and 0.065 f/cc after working respectively. The maximum airborne concentration of asbestos during asbestos removal work was 7.02 f/cc which was 70 times higher than the occupational exposure limit of Korea, 0.1 f/cc. This study recommends that retrospective exposure to asbestos based on various ship types and operations should be assessed.

Recently, the breakdown of online banking servers and the leakage of customer information give rise to much concern about the security of information systems in financial and banking companies in Korea. The enforcement of security for information system becomes much more important issue than earlier. However, the security reinforcement of information system is restricted by a budget. In addition, the activities' cost to secure information system from threatening are under uncertain circumstances and should be established by a human decision maker who is basically uncertain and vague. Thus, making the budget for information system is exposed to any extent of the risk for these reasons. First, we introduce brief fuzzy set theory and fuzzy AHP (Analytic Hierarchy Process) methodology. Then, the cost elements that comprise yearly budget are presented and the priorities among the cost elements are calculated by fuzzy AHP. The cost elements that are exposed to risk are evaluated from the both perspectives of the risk impact and risk occurrence possibility which are expressed as linguistic terms. To get information on the risk profiles-pessimistic, most likely, and optimistic-for each cost element, the evaluation is accomplished and the result is presented. At last, the budget ranges-minimum, mode, maximum-for each cost element are estimated with the consideration of the risk profiles.

An attempt is given to the problem of analyzing the two-way binary attribute data using the logistic regression model in order to find a sound statistical methodology. It is demonstrated that the analysis of variance (ANOVA) may not be good enough, especi

In this paper to prevent human errors analyzed the causes of railway accidents and human error in last 5 years(2007~2011). The 2nd Railway Comprehensive Safety Plan currently being implemented in the safety business for prevention of human error. The accidents are often resulted from multiple causes with hardware failure and human errors. And prevention of human error associated with the implementation details of the priority projects, 14 projects were selected by draw. Then Analytic Hierarchy Process(AHP) methodology was used to select what projects were effective to human error.

A product family design has received much attention over the last several decades, since a product family-based development shortens lead-times and reduces cost, as well as increases efficiency and effectiveness of the product realization process. It is c

게임 개발은 다른 소프트웨어와 비교해서 다양한 분야의 전문가들이 참여하여 창의적인 과정을 거쳐서 생산물을 만들어낸다. 여기에서 가장 중요시 되고 어려운 분야가 시나리오에 대한 공통되고 동일한 상황 인식이다. 기존의 소프트웨어와 비교해서 게임 개발 방법은 통일된 형태의 개발 및 정형화된 표현 방법이 미흡하다. 본 논문은 시나리오에 대한 개발 참여자들간의 정보를 공유하고 개발 후에 발생할 수 있는 잠재적인 오류 및 개발 비용을 최소화하기 위한 시나리오 표현 방법을 개발한다. 이를 위해 사건-상태-행동 그래프에 기반한 시나리오 표현 방법을 제안한다. 그리고, 게임 시나리오에 대한 충분한 토의와 정보 공유는 게임의 애매하고 창의적인 개발 특성상 유익한 과정이다.. 제안하는 방법을 Age of Empire 게임 시나리오로 표현하여 그 유용성을 보인다.

It is commonly recognized that railway is one of the representative transportation and it offers public service based on strategies for being rapid, automation, safety. Since the opening of high speed railway, 3-hundred-million people have used it and acknowledged its efficiency. However, derailed accident at Kwangmyeong station in February, 2011, frequent malfunction of KTX-Sancheon, and accidents by engineer's careless fault damaged on credibility of safety, Especially, spreaded accidents through social networking service by cell phones amplified anxiety of public, being criticized by the press. This study analyzed statistics of past accident and cases of passing-by accident, and surveyed 152 KTX captain engineers about their recognition of the accident by careless fault and experiences of possibility of occurrence for preventing engineer's careless fault and restoring trust According to the analysis, engineers worry about responsibility and disadvantages related to the accidents for the most, and they are nervous about malfunction for the second most. This study presents prevention methods regarding the result. First, it is required to improve mental stability and concentration on their work, secondly, advanced ability to cope with malfunction or error through repetitive education and training are required to increase confidence, and for the last, improvement of operational supporting system such as ATP, GPS to prevent errors by human factors. Improvement of the system is expected to lead engineers to prevent careless fault and regain the reputation of railway.

본 연구의 목적은 노인 환자를 대상으로 음악 게임을 활용한 치료에서 음악 게임이 어떻게 노인 환자의 우울감, 자존감의 상승에 관여했는지 알아보는 데 있다. 이를 위해 경기도 노인 전문 용양병원 양원에 입원중인 16명의 노인을 대상으로 각각 8명씩 실험집단과 통제집단을 구성한 후, 실험집단을 대상으로 음악 게임을 활용한 치료를 10회기에 걸쳐 시행하였다. 실험집단과 통제집단에게 사전-사후에 인지기능, 우울감, 자아존중감 수준을 측정하였고, 실험집단에게 10회기 동안의 게임실험 과정을 기록하고 사후 몰입도를 측정하였다. 연구 결과 게임이 제공하는 청각, 시각, 촉각 등의 감각자극과 상호소통적 활동이 노인의 우울감과 자존감 상승에 기능하였을 뿐만 아니라 손기능 향상에도 긍정적 영향을 미친 것을 알 수 있었다.