우리는 제주도 대정읍에서 뼈나이테법(skeletochronology)을 사용하여 맹꽁이(Kaloula borealis)의 나이구조를 알아보았다. 조사는 번식기인 2009년 6월에 실시하였다. 측정된 개체수는 수컷이 38개체, 암컷이 58개체로 각 개체의 SVL(snout-vent length)과 체중을 측정하고 나이구조를 분석하였다. 수컷의 평균 SVL은 43.69±3.44 mm이며, 암컷은 46.32±4.23mm로 나타나고 있다. 첫 번식에 참여하는 수컷은 2살이고, 암컷은 3살로 나타났다. 가장 나이든 수컷은 8살이며 암컷은 10살이다. 그러므로 이 종의 수명은 수컷에서는 8살, 암컷에서는 10살 이상으로 볼 수 있으며 암수의 수명에서 암컷이 더 오래살고 있다. 또한 이 값은 통계적으로도 유의미하게 나타나고 있다. 성장곡선은 von Bertalanffy growth model을 사용하였으며, 성장계수(K)는 수컷이 0.56이며 암컷은 0.41이다. 최대성장크기는 수컷이 46.41 mm이며 암컷은 50.22 mm로 나타났다.

본 연구는 고라니(Hydropotes inermis argyropus)의 식이습성을 밝히기 위하여 강원도 철원지역과 전라남도 동부지역 등에서 채집된 44개체 고라니의 위내용물 샘플에 대한 먹이식물을 분석하였다. 분석 결과, 총 15목 18과 13종의 먹이식물이 확인되었다. 그중 가지과(Solanceae, 53.8%)와 벼과(Gramineae, 30.3%)의 식물이 가장 높게 나타났다. 지역별로 살펴보면, 강원도 철원지역에서는 13목 15과 10종이 확인되었는데 초본류(70.21%)와 가지과(29.18%)의 식이물이 많은 것으로 나타났다. 또한 전라남도 동부지역에서는 8목 7과 4종이 확인되었고, 벼과(78.6%), 목본식물(14.70%)이 많은 것으로 나타났다. 계절별로는 봄(80.6%)과 가을(68.8%)에는 벼과의 식물이 여름에는 콩과 (Leguminosae, 75.9%), 겨울에는 가지과(85.4%)의 식물을 선호하는 것으로 나타나 계절에 따라 먹이원이 서로 다른 양상을 보였다. 따라서 고라니는 지역별, 계절별로 선호하는 식물이 다르게 나타난 바 고라니 관리방안에 본 결과를 활용할 수 있다.

본 연구는 디자인 요소로 한글을 사용하려 할 때 다양한 한글글꼴에 대한 감성과 선호도에 대한 객관적 정보 제공하여 한글글꼴 사용에 있어서의 방향 제시를 돕고자 하는 목표를 가지고 있다. 연구의 세부 목표는 첫째, 한글글꼴의 조형 요소를 좀 더 객관적으로 분석하기 위해서 한글을 전혀 모르는 중국인을 대상으로 한글글꼴에 대한 감성 차이가 한국인하고 어느 정도 차이가 있는지 분석하였다. 둘째, 글꼴에 대하여 한국인과 중국인들의 선호 비선호를 비교 분석 하였다. 첫 번째 목표를 밝히기 위해 요인 분석을 실시한 결과, 한국인과 중국인이 모두 2가지 대표요인으로 [친숙성], [soft]가 추출되었으며 양국 모두 제 1요인과 제2요인의 소속요인 모두가 순위의 차이는 있었지만 대다수가 공통적으로 가지고 있어 한국인과 중국인들이 한글글꼴에 대하여 서로 비슷한 감성을 가지고 있음을 알 수 있다. 두 번째 한글글꼴 선호도 분석을 실시한 결과, 한국인 및 중국인의 선호도를 결정하는 가장 큰 요인은 친숙성이라고 볼 수 있고, 한글을 전혀 모르는 중국인들 또한 중국문화에 애용하여 왔던 붓글씨적인 서체를 선호하는 경향이 있었다.

우리나라 재래종벼 6품종의 벼 흰잎마름병 저항성 유전자를 동정하고자 저항성 유전자 Xa1를 갖는 IR-BB 101과의 교배조합을 양성하고 일본균주 ⅠA(T7174)를 접종한 결과 청군벼의 벼 흰잎마름병 저항성 유전자는 Xa1 이었고, 육성재래, 아국도, 흑피 및 이천7일찰은 Xa1 및 다른 우성유전자 1개를 보유하였으며, 긴까락샤레는 Xa1 및 다른 우성유전자 2개를 갖고 있었고 이 중 2개는 저항성 발현에 보족적으로 작용하는 것으로 추정되었다.

미얀마는 국토면적 676,577㎞2로 한반도 전체 면적의 3배에 달하여, 동남아시아 대륙 국가 중에서는 가장 큰 국가이다. 이러한 지형을 바탕으로 남북 및 동서로 다양한 기후대가 나타나 특유의 농업생태환경이 조성되어 있다. 미얀마에서 농업은 가장 중요한 산업이며 그중 벼농사가 차지하는 비중이 제일 크다. 쌀 생산량은 꾸준히 증가하여 최근 2009/10년도 약 3268만 톤 생산되었다. 최근 미얀마에서 가장 많이 수출되는 품목은 콩이며, 그 중 생산량이 가장 많은 Black gram은 2009/10년도 약 151만 톤 생산되었다. 주요 생산하고 있는 기타 작물로는 밀, 옥수수, 수수가 있으며 밀과 옥수수의 재배면적과 생산량은 꾸준히 증가하고 있다. 미얀마에서 중요한 농산물중 하나인 참깨는 유료작물 중 가장 넓은 면적에 재배되어지고 있으며 미얀마의 주요 수출입국으로는 태국, 인도, 중국 등이다.

The increase in the consumption of herb medicines have made their use a public health problem due to the potential fungal contamination and the risk of the presence of mycotoxins. 360 samples of herb medicines were evaluated for the aflatoxin contamination. The natural occurrence of aflatoxins in these samples were determined using immunoaffinity column clean up and high performance liquid chromatography (HPLC) with post-column derivatization. For samples analyzed, mean levels (incidence) of AFB1, AFB2, AFG1 and AFG2 in positive samples were 1.4 μg/kg (46.4%), 0.4 μg/kg (25.4%), 1.1 μg/kg (37.8%) and 0.9 μg/kg (24.3%), respectively. Recoveries of the full analytical procedure were 71.7~99.7% for AFB1, 88.1~99.2% for AFB2, 82.8~95.5% for AFG1 and 77.9~90.0%for AFG2. The excess cancer risk estimated using the cancer potency of aflatoxin B1 (7 (mg/kg/day)−1 for HBsAg− and 230 (mg/kg/day)−1 for HBsAg+) were 1.30 × 10^(−5) ~ 1.22 × 10^(−7) for hepatits B surface antigen negative (HBsAg−) and 3.31 × 10^(−4)~ 3.12 × 10^(−6) for hepatits B surface antigen positive (HBsAg+) respectively. In conclusion, although the contamination levels of samples used in the study were low, further actions are also required to undertake a program of herbal surveys in order to access mycotoxin contamination overall so that the safety of public will be protected.

Cereals are the main raw material for sunsik manufacture. As the fundamental processing step, it is very important to confirm the level of the microorganism contamination in the cereals. This study was carried out a micrbiological screening of cereal samples for sunsik from 19 companies located in South Korea. Ten kinds of cereals which were glutinous rice, barley, brownrice, blackbean, blackrice, blacksesame, sorghum, millet, perilla seed, and adlay were investigated. As the results, the contaminations of general bacterial were 3.1~8.6 log(CFU/g). The results of Escherichia coli were 1.0~3.4 log(CFU/g). There was no contamination of Salmonella. spp in any cereal samples except black sesame and mold was detected in barley. The experiment for microbiological contamination during sunsik processing was also investigated in this study. The results of general bacteria were detected as 5.1~8.5, 4.4~7.5,1.0~2.3, 2.4~4.2, 1.0~4.0, 3.4~4.2, 4.3~5.2, and 3.3~5.5 log(CFU/g) during environment of warehousing, washing,steaming, 1st cooling, drying, 2nd cooling, grinding, and packaging process, respectively. The results of coliform were 1.0~2.0 log(CFU/g) during warehousing respectively. Mold was found in warehousing. In case of the instruments, the contaminations of general bacterial were 4.2~7.5, 0.1~2.0, 0.1~3.2, 3.7~4.0, 2.5~3.0, and 3.8~5.2 log(CFU/g) in cereals tanks, washing machines, grinding machines, packaging machines, and workrooms. The results of coliform were 2.4~4.0, 0.0~4.1 log(CFU/g) in cereals tanks and grinding machines, respectively. Mold were only found in cereals tanks, grinding machines, and workings. Therefore, the risk of hazard microorganisms contmination might be decrased as the exhaustive management is applied to the whole sunsik process.

In this study, the contamination levels of total aerobic bacteria, E. coli, total coliform and S. aureus of seasoned dried fishes (SDF) in Korea were investigated. A total of 81 SDF samples were purchased randomly from 28 stores. Contamination range of total aerobic bacteria, total coliform and S. aureus were 150~1,700,000, 10~31,000and 10~220 CFU/g, respectively. E. coli was detected in only one samples in the qualitative test. We have analyzed quantitatively Staphylococcal enterotoxins (SE-type A, C and D) produced by S. aureus contaminated in SDF using a TECRA kit and standard curve. The curve equation was Y = 0.1499 * X + 0.1183 and maximum amount of SEs in SDF was 0.71 ng/ml. Reduction speed of S. aureus in SDF stored at 37 ℃ was the highest among the samples stored for 8 days at different temperature of 7, 18 and 37℃ . On the basis of the results, SDF in Korea can be contaminated by a variety of pathogenic bacteria. Therefore, precautionary measures are necessary for consumer protection, including the improvement of sanitary conditions in the processing plants in Korea.

This study surveyed and compared the temperatures established in display stands and food surfaces for cold and frozen foods in large discount stores in Korea. The temperatures established in display stands for cold food ranged with 3.5 ± 1.8 ℃ as mean, minimum and maximum were 0 ℃ and 7 ℃ . However, the surface temperatures of cold food on sale ranged with 10.7 ± 2.9 ℃ as a mean, minimum 4.6 ℃ and maximum 18.4 ℃ . Totally, the surface temperature of cold food on sale was 7.2 ℃ , as a mean, higher than established in display stands for cold food in large discount stores in Korea. 53% of the surveyed cold foods were more than 10 ℃ in surface temperature and only 47% was less than 10 ℃ . The differences between temperatures were lowest in fruits, salads and vegetables, but highest in milk products. On the other hand, the temperatures established in display stands for frozen food showed a range with −20.7 ± 1 ℃ as a mean. However, the surface temperatures of frozen food on sale showed a range with −15.4 ±5 ℃ as a mean, minimum −28 ℃ and maximum −4.6 ℃ (included defrosting). The surface temperatures of frozen food,frozen meats, frozen processed foods and ice creams were −13.8 ℃ , −15.9 ℃ , and −16.8 ℃ , respectively. Only 32.3%of surveyed frozen foods showed less than −18 ℃ in surface temperature. In conclusion, the temperatures established on cold and frozen food display stands were less than those of cold and frozen food surfaces on sale. There was also much variation in food surface temperatures during cold and frozen food storage and sales. Therefore, a temperature management system technology use at the distribution level for cold and frozen foods will be developed.

One of the most important objectives of post-marketing monitoring of dietary supplements is the early detection of unknown and unexpected adverse events (AEs). Several causality algorithms, such as the Naranjo scale, the RUCAM scale, and the M&V scale are available for the estimation of the likelihood of causation between a product and an AE. Based on the existing algorithms, the Korea Food & Drug Administration has developed a new algorithm tool to reflect the characteristics of dietary supplements in the causality analysis. However, additional work will be required to confirm if the newly developed algorithm tool has reasonable sensitivity and not to generate an unacceptable number of false positives signals.

We surveyed the sanitary conditions for 17 cold and frozen food storage warehouses in Korea,using the following 5 inspections items: “putting into warehouse (A)”, “prevention of cross-contamination (B)”, “storage management (C)”, “temperature control (D)”, and “management of records and documents (E)”, We included 20detailed items. The results of distribution for frequency by five major inspection items showed that “(E)” was the highest,the next “(D)”, “(C)”; and “(B)” was the lowest. In the correlation of inspection scores between total scores, “(B)”and “(C)” were highly related to the total score, therefore, the higher score of “(B)” or “(C)”, the higher for the total score. In details of inspection items, “the management of cross-contamination upon taking product out of the warehouse”had the lowest score with a mean, of 2.67 ± 1.80, and also ranked as first of the 20 items.

목 적 : 안정피로 개선을 위해 고안된 조절이완장치를 사용하여 VDT 작업과 관련한 임상성능을 분석하 고자 한다. 방 법 : 성인 30명(26.5±4.8세)을 대상으로 조절이완장치(EK-075/30, 에스테크(주), 한국)를 사용하지 않은 상태에서 VDT 작업 전과 후 그리고 조절이완 장치를 착용 상태에서 VDT 작업 후에 굴절이상도, 원거 리와 근거리의 시력 및 수평사위도, 폭주근점, 조절근점, 양안 및 단안 조절용이성, 융합버전스를 비교분석 하였으며, 총 4단계의 설문을 통해 대상자의 전반적인 건강상태와 VDT 작업 관련 기본사항, VDT 작업 중 느끼는 자각증상, 기기착용과 관련한 사항을 설문하였다. 결 과 : 조절이완장치를 사용하지 않은 상태에서 VDT 작업 후 굴절이상도가 유의하게 증가되었고 근거 리 시력이 감소하는 것으로 나타났으며, 원거리 사위도와 근거리 사위도는 내사위 방향으로 유의하게 증가 하였고 조절용이성은 양안과 단안 모두 유의하게 감소하였다. 조절이완장치를 사용한 상태에서의 VDT 작업 후 굴절이상도, 원거리 시력, 근거리 시력, 원거리 사위도는 통계적으로 유의한 차이를 보이지 않았으나 근 거리 사위도는 내사위 방향으로 증가하였고 단안 조절용이성도 유의하게 증가하였다. 결 론 : 조절이완장치의 착용으로 VDT 작업 후의 일시적인 근시화와 내사위의 증가가 감소되고 조절용 이성이 증가된 결과로 보아 조절과 조절이완의 훈련 효과가 있는 것으로 사료된다. 조절이완장치의 착용이 근거리 작업과 관련한 안정피로를 경감시키고 근거리 작업효율을 향상시키며 나아가 삶의 질 향상에도 기여 할 것이라고 생각된다.

이 논문에서는 부트스트래핑 DEA 모형을 이용하여 품목농협의 효율성 분석 값의 통계적 유의성을 분석 하였다. 분석결과, 첫째, 일반적 DEA모형에 의한 기술효율성은 0.878로 추정된 반면, 부트스트래핑 기 법을 적용하면 0.804로 추정되었다. 그러나 두 값의 차이는 신뢰구간 범위 내에 있기 때문에 통계적으로는 유의하지 않다. 또한 95% 유의수준하에서 기술효율성의 통계적 신뢰수준은 0.726에서 0.874로 분석되었다. 둘째, 일반적 DEA모형에서 효율적인 품목농협으로 추정된 19개 농협 모두 부트스트래핑 기법을 적용한 경우 비효율적인 것으로 추정되었다. 이는 일반적 DEA모형의 경우 비효율적인 품목농협이 효율적인 것으로 추정될 수 있다는 것이다.

A rapid, simple and reliable LC-MS/MS method, which can be used on a routine basis, was developed for the simultaneous detection of 8 penicillin antibiotics (amoxicillin, ampicillin, penicillin-G, penicillin-V, oxacillin, cloxacillin, nafcillin and dicloxacillin) in swine muscle and kidney. The antibiotics were extracted from samples with water and methanol. The extract was centrifuged, filtered and analyzed by liquid chromatography coupled with a tandem mass spectrometer (LC-MS/MS), using a C18 reversed phase column with water/acetonitrile gradient containing 0.05 % formic acid. Mass spectral acquisition was achieved in an electrospray positive ion mode by applying multiple reaction monitoring (MRM) of 2 fragment ion transitions to provide a high degree of sensitivity and specificity. The ion rations were consistent and could be used for confirmation of identity of the penicillin antibiotics. Recoveries of eight penicillins at three fortification levels (10, 20 and 40㎍/㎏) ranged from 79.8 to 102.4% and 72.8 to 103.4% in swine muscle and kidney, respectively. The coefficient of variation was than 9% in all samples. The estimated limits of quantification (LOQs) ranged from 1.0 to 3.2㎍/㎏ in swine muscle and kidney, respectively. The LOQs of this method are below the MRLs of penicillin antibiotics in animal tissues established in Korea and other countries.

목 적: 초등학교 고학년을 대상으로 생활습관에 따른 근시 정도를 분석하여 근시의 관련 요인을 알아보 고자 하였다. 방 법: 2009년 9월부터 2010년 5월까지 서울과 아산에 위치하고 있는 8개 초등학교의 남녀 학생 중 안 질환과 사시나 약시가 없는 257명을 대상으로 자동굴절력계를 사용하여 굴절이상도를 검사하였다. 시력관리 에 대한 항목으로 시력교정 상태, 현재 착용하는 안경의 눈 검사 시기와 안경의 착용 실태에 대해서 조사하 였고, 생활습관 관련 항목은 컴퓨터 사용시간, 독서 시간과 독서 자세, TV 시청 자세, 1일 실외 생활시간 그 리고 부모의 근시 여부를 포함하였다. 대상자의 등가구면굴절력은 -9.38D~+0.38D로 평균연령은 12.74±0.44세(12~13세), 남녀대상자는 남 99명(40.7%)와 여 144명(59.3%) 이었다. 결 과: 전체 대상자들의 평균 등가구면굴절력은 -2.01±1.86D로 지역별 차이는 없었다(서울: -1.87± 0.49D, 충남: 평균 -2.09±1.86D, p=0.374). 전체 대상자 중 안경을 착용하지 않는 그룹에서 72.5%가 근 시였고, 안경착용 그룹에서는 눈 검사를 받은 지 6개월이 지난 학생이 34.6%였다. 성별, 1일 2시간 이상의 독서 시간과 소파, 바닥, 거실바닥에 엎드려 보는 나쁜 독서 자세, TV 시청 자세, 1일 실외에서의 생활시간 에 따라서 근시의 정도는 통계적으로 유의한 차이가 없었다. 1일 컴퓨터 사용시간이 2시간 이상(2시간 미만: -1.80±1.68D, 2시간 이상: -2.36±2.07D, p=0.026)일 경우에는 근시의 경향이 높았고, 부모 모두 근시가 있을 때 자녀의 굴절이상도는 평균 -2.78±2.36D, 둘 중 한명만 근시일 경우는 -1.95±1.75D, 둘 다 근시 가 없을 때는 -1.60±1.44D를 보였다(p=0.001). 결 론: 1일 컴퓨터 사용시간과 부모의 근시 여부에 따라 초등학교 고학년 학생의 근시 정도가 높아졌다.

In order to ascertain the extent to which Korean college students are familiar with the proper use of the English comma, this study examined all the instances of the comma used in a learner corpus consisting of 787 compositions written by Korean college students. All in all, 5,869 tokens of comma errors were identified and subsequently grouped into five categories of errors: (a) omission, (b) addition, (c) wrong substitution, (d) wrong position, and (e) others. A careful examination of these five types of comma errors revealed that the number of tokens in omission was the highest among the five categories, accounting for over half of all the comma errors, and that the students who received higher scores on their essays were just as likely to make comma errors as those who scored lower, a fact which suggests that students with a higher procifiency of English also need instruction on using the comma properly. These results lead to the conclusion that most Korean students are not familiar with contextual uses of the comma and that teaching the comma should be incorporated into English education both at the secondary and at the tertiary level.

Rabies is a zoonotic disease that causes severe destruction to the central nerve system which is usually fatal. It is one of the most important disease around the world and particular in Asia because of the high costs of prevention and post-exposure treatment. After the recurrence of sylvatic rabies in 1993, the number of raccoon dog mediating rabies cases in Korea has maintained annually until 2011. To better understand the current rabies epidemics in Korea, Korean rabies isolate (SKRBV0601GY) from Gyeonggi province in 2006 was compared with previous isolates in Korea and with isolates originating from the North-East Asia, such as Japan, China and Russia, based on complete nucleoprotein (N) gene sequences. By comparison of the N genes among these viruses, SKRBV0601GY revealed that nucleotide similarity ranged from 97.7 to 99.7%, 96.4 to 97.5%, 91.4 to 96.3%, 89.2 to 90% and 86.1 to 88.1% with Korean isolates, "Arctic-like-2" viruses, "Arctic" viruses, Russian group C - E and Chinese isolates, respectively. Phylogenetic analyses of the isolates revealed that the Korean isolate in 2006 belonged to Korean group B. The topology of the phylogenetic tree of Korean isolates related not the species and year of isolation but the geological location of the virus isolates. All of the Korean isolates showed close relationship to the "Arctic-like-2" virus (Russian group B) more than the "Arctic" virus (Russian group A) and all of the Chinese isolates (Chinese group A, B and C). The "Arctic-like-2" virus group contains the Japanese isolate and Russian group B viruses, originating from the south of East Siberia and Far East in Russia. These molecular data demonstrated that the current rabies epizootic in Korea developed independently of Chinese groups and originated from the "arctic-like-2" viruses in detail.

Random amplified polymorphic DNA (RAPD) and fluorescent amplified fragment length polymorphism (FAFLP) analyses were executed on a total of 28 Salmonella spp., including 6 ATCC reference strains, 2 isolates from outbreaks of food poisoning in Gwangju, and 20 isolates from carcasses. For RAPD analysis, four primers, named P1254, 23L, OPA-4, OPB-17 were used producing amplification fragments ranged from 0.18kb to 2.6kb. As a result, 5 types using P1254, 5 types using 23L, 3 types using OPA-4, and 6 types using OPB-17 and a total of 18 RAPD types were achieved. For FAFLP analysis, bacterial genomic DNA was digested with endonucleases EcoRⅠ and MseⅠ, site-specific adaptors were ligated, and PCR amplification was carried out with an EcoR1 adaptor-specific primer labelled with fluorescent dye. Amplified fragments, which were separated on a polyacrylamide sequencing gel ranged from 35bp to 300bp were analysed. Results were displayed as a dendrogram with genetic distance. Twenty two Salmonella isolates and 6 reference strains were divided into 14 groups in a level of 0.136 genetic distance. In conclusion, Salmonella isolates of chicken carcasses have different genetic properties when compared to reference strains and isolates from outbreak of food poisoning.

The purpose of this study is to describe foreign students' Korean language pragmatic competence in relation to speech exchange and communicative strategy exposed in the process by which a communicative task was effectively completed. This study designed and applied ‘information-gab tasks' like ‘picture-description tasks' for the realization of ‘referential communication' in which two speakers give and take the explanation about a specific object in a state where neither of them mutually share adequate information about it. As a result of observing the dialog data elicited by the task, it was found that learners were arriving at the co-construction of ‘identification of referent' and ‘role-taking' in various communication modes. At this point, the introductory/intermediate learners had difficulty carrying out such an interactive component in performance of the task. Even the type of feedback varied between the introductory/intermediate learners and the advanced learners. The utterance clarifying the difference in the photos was generally not revealed in a verbal sequence. The linguistic form of ‘-in geot gatda(seem to)' was frequently used with the effect of assumptive judgment strategy.