J. M. 싱의 시학 읽어내기: 「아란 섬」과 「바다를 타는 사람들」에 나타난 자연과 시 우리말 요약: J. M. 싱은 아마 지금까지 아일랜드에서 태어난 가장 위대한 극작가들 중의 하나이다. 그러나 그는 자주 많은 이들에 의해 폄하(조이스 포함하여)되고 있지 만 예이츠는 당대에 모든 극작가들 중 최고로 여기는 것 같다. 본 논문은 싱이 아란 을 방문하기 전에 이미 잘 준비된 위대한 극을 쓸 수 있었다고 주장하며, 「아란 섬」을 증거로 제시하는데, 그가 나중에 희곡을 쓸 때 사용하는 관찰, 느낌, 생각으로 가 득 찬 아주 시적인 위대한 저술임을 증명한다

본 연구에서는 자발적인 웃음과 인위적인 웃음을 변별하는 데 있어서 일반 사람들의 정확도와 컴퓨터를 이용한 분류 알고리즘의 정확도를 비교하였다. 실험참가자들은 단일 영상 판단 과제와 쌍비교 판단과제를 수행하였다. 단일 영상판단 과제는 웃음 영상을 한 장씩 제시하면서 이 영상의 웃음이 자발적인 것인지 인위적인 것인지를 판단하는 것이었으며, 쌍비교 판단과제는 동일한 사람에게서 얻은 두 종류의 웃음 영상을 동시에 제시하면서 자발적인 웃음 영상이 어떤 것인지 판단하는 것이었다. 분류 알고리즘의 정확도를 산출하기 위하여 웃음 영상 각각에서 8 종류의 얼굴 특성치들을 추출하였다. 약 50%의 영상을 사용하여 단계적 선형판별분석을 수행하였으며, 여기서 산출된 판별함수를 이용하여 나머지 영상을 분류하였다. 단일 영상에 대한 판단결과, 단계적 선형판별분석의 정확도가 사람들의 정확도보다 높았다. 쌍비교에 대한 판단결과도 단계적 선형판별분석의 정확도가 사람들의 정확도보다 높았다. 20명의 실험참가자 중 선형판별분석의 정확도를 넘어서는 사람은 없었다. 판별분석에 중요하게 사용된 얼굴 특성치는 눈머리의 각도로, 눈을 가늘게 뜬 정도를 나타낸다. Ekman의 FACS에 따르면, 이 특성치는 AU 6에 해당한다. 사람들의 정확도가 낮은 이유는 두 종류의 웃음을 구별할 때, 눈에 관한 정보를 충분히 사용하지 않았기 때문으로 추론되었다.

Recently, extensive research has been performed in the field of orthopedic medicine to develop cell-based therapies for the restoration of injured bone tissue. But there has been rarely reported about rehabilitaton of oral and maxillofacial bone defect using self-derived osteoblasts. Normal human osteoblast cell(NHost) was previously established into marrow-derived human mesenchymal stem cells for their capacity to proliferate and differentiate into osteoblasts under various culture conditions. The purpose of this study was to examine proliferation and differentiation of NHosts effected by growth factors with ALP activity and RT-PCR. After NHosts were cultured under basal and osteogenic medium at 37℃ and 5% CO2, they were analyzed by ALP activity and RT-PCR. BMP-2 under osteogenic medium decreased growth rate of NHosts compared to under osteogenic medium. BMP-2 under osteogenic medium induced osteoblastic differentiation in NHosts by increased ALP activity. The differentiating capacity of NHosts under osteogenic medium showed that NHosts expressed higher mRNA expression levels of OSX and OCN, while that of RUNX2 decreased after BMP-2 treatment. It suggested that NHosts having characteristics of osteoprecursor cells might be more advanced in their osteogenesis development by BMP-2, making NHosts an interesting biological tool for treatment of skeletal defects and diseases of oral and maxillofacial bone.

The purpose of this study was to assess the influence of scapular alignment on the electromyographic (EMG) activity of the trapezius in people with a short pectoralis minor muscle. For the study, we recruited 15 volunteers who had positive results for short on a pectoralis minor muscle length test. We measured the EMG activity of the upper, middle and lower trapezius muscles. The participants lifted their dominant arm to ear level with the thumb up toward the ceiling in the prone position on a table with the shoulder at a flexion angle of 180 degrees and a horizontal abduction angle of 120 degrees. Scapula was manually aligned by an experienced physical therapist prior to arm lift for the scapular alignment condition. A paired t-test was used to compare the effects of scapular alignment on the EMG activity of the trapezius muscles. The EMG activity of the lower trapezius muscle was significantly increased during the test with the scapular alignment compared to that without scapular alignment (p<.05), while the upper trapezius and middle trapezius exhibited no significant difference between the two conditions (p>.05). The findings of this study suggest that a scapular alignment may alter the recruitment of the lower trapezius muscle during arm lifting in the prone position in people with a short pectoralis minor muscle.

Cruciferous vegetables including diindolylmethane (DIM) have been shown to have anticancer activity. Especially, DIM-pPhBr and DIM-pPhF used in this study was reported to have more effective and less toxic effects than DIM. However, there is no report presenting their anti-tumorigenic activity in oral cancer. In the present study, we examined the effects of DIM-pPhBr and DIM-pPhF on the cell proliferation and apoptosis in KB human oral cancer cells. DIM-pPhBr and DIM-pPhF decreased cell proliferation and induced apoptosis evidenced by western blot analysis, DAPI staining and sub-G1 population. This provides the first evidence that DIM-pPhBr and DIM-pPhF originating from cruciferous vegetables induce apoptotic cell death in human oral cancer cells to inhibit cancer cell proliferation.

It was conducted the experiment, divided into three groups as normal, poor and polycystic ovary syndrome, to detect the change of protein patterns in follicular fluid on ovarian response following controlled ovarian hyperstimulation for human IVF outcome. In the normal group, it was confirmed reproducible 57 spots in the detected total 81 spots. Then 1 spot was not found in the other groups. In the poor responder group, it was found reproducible 53 spots in the detected total 98 spots. 6 spots were down-regulation and 7 spots were up-regulation comparable with normal group. There were not 5 spots in poor responder group comparable with other groups. In the polycystic ovary syndrome group, it was expressed reproducible 53 spots in the detected total 80 spots and 3 spots were just expressed in this group. However, 4 spots were not found in polycystic ovary syndrome. 9 spots were up-regulation comparable with normal group. Significant up and down-regulation spots among the each groups were identified. The results were a cytosolic carboxypeptidase, a signal-induced proliferation-associated protein 1, a ceruloplasmin, a keratin(type Ⅱ cytoskeletal 1), a polypeptide N-acetylgalactosaminyltransferase 2, a serine/threonine-protein phosphatase 4 regulatory subunit 4. It was identified that 8 spots, 6 kinds of protein are corresponded with NCBInr database research, but 10 spots were failed in the identification. In conclusion, it has been confirmed change and expression of protein on the ovarian response following COH of human.

Plasminogen activators(PA) such as urokinase(uPA) and tissue type plasminogen activators(tPA), physiologically catalyze the conversion of the plasminogen to the wide spectrum proteinase plasmin. Because uPA and tPA are involved in cell growth, differentiation and migration of oral cancer, oral epithelial carcinogenesis including transformation of precancerous lesion into oral squamous cell carcinoma with PA is very interesting. It is important to prevent precancerous condition from transfoming into oral squamous cell carcinoma by the inhibitory effect of various drug. It is well known that cyclosporine A(CsA) as immunosuppressive properties exerts anti-cancer effects. Recently it is widely accepted that cultured immortalized oral keratinocyte (IHOK) is considered as an intermediate stage of oral carcinogenesis and used as precancerous condition in vitro. Thus it was thought that it might be interesting to investigate CsA effect on PA expression of IHOK. IHOK was cultured under KBM bullet kit at 37℃ under 95% CO2 incubator. Subconfluent IHOK cells was treated at different CsA concentration. uPA and tPA protein expression from cultured IHOK cell line has been detected by ELISA analysis in the CsA-treated samples. uPA expression of IHOK was higher than that of NHOK, while tPA was similar to that of NHOK. After CsA treatment, CsA might not effect the expression of uPA of IHOK, while showed a little effect on tPA of IHOK. It suggested that CsA had no effect in uPA expression of IHOK although uPA could be used as a marker for precancerous lesion.

Cyclosporin A(CsA) as immunosuppressive drug is used to prevent immune reactions after organ transplant. And also It is reported that the effect of CsA on osteoblast differentiation has been controversial according to dosage. The purpose of this study was to examine the effect of various CsA concentrations on osteoblast differentiation. According to different concentration o f CsA, growth curve, apoptosis index MTT assay, ALP activation and osteocalcin secretion, in cultured NHost were analyzed. Treating osteoblasts with low concentrations of CsA increased growth rate, MTT assay activity, ALP activation and osteocalcin protein levels in a dose-dependent manner, while high concentration showed opposite results. Therefore, these results showed that low concentrations of CsA increased osteoblast differentiation, while high concentrations elicited an opposite response, showing inhibition of CsA on osteoblast differentiation. It suggested that different CsA concentrations might play in regulating NHost differentiation, and its specific activation of lower concentration will represent a viable anabolic therapy for bone resorption disease in future.

In the previous molecular cloning study from human salivary gland cDNA l ibrary a novel clone (C77-091) was known as a candidate gene for antimicrobial protein by GenBank database search and RNA in situ hybridization. This study is aimed to identify the molecular characteristics of C77-091 protein, which showed an antimicrobial activity on E.coli, thereby named as salivary antimicrobial protein (SAMP). SAMP consisted of a typical hydrophobic amino acid rich domain in the N-terminus, a cluster of basic amino acids, carbohydrate attachment site, a possible transglutaminase catalyzed cross-linking site, and multiple consensus sequences of phosphorylation site in the C-terminus. Western blot analysis of human organs and tissue with the monospecific antibody to the synthetic SAMP peptide showed strong interacting protein from the extracts from submandibular gland and parotid saliva but absent in the mixed saliva, and the immunohistochemical staining detected a strong positive regions in the secretory granules in the luminal cytoplasm of interlobular ductal cells of salivary gland. The SAMP was also distributed in the human sebaceous gland and prostate. These data suggest that C77-091 named SAMP gene is a novel antimicrobial protein in human salivary gland, which may play a role for the innate immunity by protecting and stabilizing the mucosal epithelium to maintain homeostasis of oral mucosa.

Many methods have been developed for more efficient gene delivery and expression in human cells. A number of studies have been performed in achieving successful gene delivery and expression conditions. We investigated differential gene expression patterns after delivery adenoviral vector containing green fluorescent protein(GFP) gene into human cancer cell lines. We constructed recombinant adenoviral Ad-CMV-GFP containing CMV promoter and GFP gene. The efficiency of gene expression was assessed by observation GFP expressing cells using fluorescent microscopy after transfer of Ad-CMV-GFP in concentrations of 0.1μl. 1μl. 10μl. At first, we evaluated expression patterns of gene in several human cancer cell lines, gastric adenocarcinoma cell line AGS was showed high level of GFP expression compared with colorectal adenocarcinoma cell line HT-29. After transfer 0.1μl of Ad-CMV-GFP in AGS, we could found that GFP expression cells were observed in next day and highly increased 2 days. While, small number of GFP expressing cells were examined in HT-29 and SNU-C4. Therefore, these data showed that AGS was expressed the highest level of GFP and almost AGS cells seems to express GFP in concentration of 1μl of Ad-CMV-GFP. GFP expression pattern in HT-29 reveal that expression was low in next day after gene transfer but significantly increase expression level in 2 days. In case of SNU-C4, GFP expression increased with increasing concentration of Ad-CMV-GFP and t ransfer times. For examine effects of transfer times in small amount gene, we transfer in concentration of 0.1μl Ad-CMV-GFP and detected GFP expression patterns after 2 days or 4 days. As a result, expression level of GFP in AGS was increase about 2 fold after 4 days compared with 2 days, but any difference of GFP expression levels were not showed in HT-29 and SNU-C4. Our study suggested that adenovirus was very efficient gene transfer vector for gene expression in human cancer cell lines. In addition to, we also demonstrated that gene expression patterns was dependent on each human cell lines. Therefore, further studies will be needed to confirm the optimum conditions for efficient gene delivery and expression in each target cell lines with consideration to cellular properties.

In order to unravel unidentified genes from human salivary gland, a cDNA library of human submandibular gland was constructed in the Uni‐ZAP XR vector by use of mRNA from human submandibular gland and ZAP‐cDNA® Gigapack® III Gold Cloning Kit. cDNA of salivary gland was subtracted with cDNA of immortalized human keratinocyte cell line, Rhim Human Epithelial Keratinocyte cell line. The phage cDNA library was converted into a pBluescript phagemid cDNA library, which was subsequently plated on LB plates with ampicillin, IPTG, and X‐gal, and white colonies were selected for sequencing. Among 200 clones analyzed, four clones containing C77‐091, C75‐014, C76‐022, and C76‐012 designated orphan genes that are intensely expressed in the interlobular ductal and serous acinar cells of human submandibular gland. Particularly C77‐091 gene expresses 46 amino acids peptide (pI=9.45). C75‐014 and C76‐022 genes were characterized as those expressing excretory basic proteins primarily consist of alanine, proline, and leucine residues, mimicking a basic proline‐rich protein (bPRP) showing helical structures and having multiple consensus sequences of phosphorylation sites. The strong expression of C76‐012 mRNA in the nuclei of salivary ductal and acinar cells suggests a role of C76‐012 gene as a DNA binding RNA/protein. These data suggest that the identification of four orphan genes from the human salivary glands may add further understanding of greater role of salivary proteins providing innate immunity by protecting and stabilizing the mucosal epithelium in the maintaining homeostasis of oral mucosa.

The purpose of this study was to evaluate the biological characteristic of deproteinized freeze dried bovine bone(DFDBB) through grafting to maxillary sinus as following time lapsed. Nine patients who were needed of sinus elevation procedure because of severe resorption of maxillary edentulous alveolar bone were selected. patients were divided into three group. Firstly sinus lifting procedure was performed and then the implantation procedure was performed after 6 months in first group, 12 months in second group and 18 months in third group and simutaneously tissues of sinus were obtained by trephine. 18 sections are made from every obtained tissue. 9 sections were stained by Masson's trichrome method and were taken a photo at 100 times of magnification. Relative area of newly formed bone were obtained by IPTK(image processing tool kit) version 5.0 program and mean values and standard deviations were produced from obtained data by using SPSS version 17 program and significance tests were conducted by ANOVA method. This study revealed that DFDBB stimulated new bone formation in maxillary sinus and did not have osteoinductive capacity but osteoconductive capacity, and DFDBB was exceedingly slowly resorbed.

The pattern of wound healing process differs markedly according to the cell types. Gingival wounds heal more rapidly without scar, however dermal wounds show collagen laid down in thick disorganized patterns and keloid formation. This h as b een s uggested t o be d ue t o the presence of d ifferent E C M components a nd c ytokines a s well a s growth factors. The purpose of this study was to examine the differential expression of genes in connection with keloid formation in gingival fibroblasts (hGFs) and dermal fibroblasts (hDFs) in response to inflammation. In this study, we investigated the differences between hGFs and hDFs in the expression and production of cyclooxygenase (COX-2), prostaglandins E2 (PGE2), transforming growth factor (TGF)-β, collagens, matrix metalloproteinases (MMPs), and tissue inhibitors of matrix metalloproteinases (TIMPs) which play important roles in collagen deposition in wound healing. The hGFs and hDFs were primary cultured and allocated to arachidonic acid (AA) treatment group and control group. Protein and mRNA were extracted right after (0 hr) and 24 hr after AA treatment. At a defined concentration of AA in hGFs and hDFs, MTT assay was performed. The mRNA and protein expression levels of COX-2, TGF-β, collagen 1 and 3, MMP 1 and TIMP 1 were examined by Real-time PCR and Western blots. The amounts of PGE2 were measured by enzyme-linked immunosorbent assay (ELISA).The expression of COX-2 and TGF-β exhibited reduced levels in hGFs , but were increased in hDFs at 24 hr after AA treatment. Production of PGE2 was increased in hGFs and hDFs at right after AA treatment but, not changed at 24 hr after AA treatment. The protein and mRNA expression of collagen 1 and 3 were decreased in hGFs , whereas increased in hDFs at 24 hr AA treatment. Expression of MMP-1 protein was increased in hGFs at 24 hr but, was decreased in hDFs at 24 hr compared with that of control. The protein expression of TIMP-1 was decreased in hGFs but, was increased in hDFs at 24 hr compared with that of control. These observations demonstrate differential expression between gingival and dermal fibroblasts in regulation of collagenolytic capacity by extracellular matrix-associated genes in keloid formation associated with wound repair.

HCI 분야에서 사용성은 시스템의 객관적인 사용성에 초점을 둔 것에서 점차 사용자들이 시스템을 사용하면서 느끼는 주관적인 경험을 중시하는 개념으로 확장, 변화하고 있다. 오늘날 대부분의 사람들은 휴대전화를 소지하고 사용한다. 휴대전화와 같이 인간과의 상호작용 빈도가 높은 인터페이스에서 보다 긍정적인 사용자의 주관적 경험을 유발하는 것은 중요하다고 볼 수 있다. 본 연구에서는 감정을 표현하는 인터페이스가 인간에게 보다 긍정적인 사용자 경험을 유발할 것이라는 가설 하에 감정을 표현하는 인간 목소리를 통해 청각적 피드백을 제공하는 휴대전화 프로토타입(prototype)을 제작한 후, 감정을 표현하기에 적절하지 않은 기계음을 통해 청각피드백을 제공하는 휴대전화 조건과의 비교를 통해 어떠한 조건에서 사용자들이 보다 더 긍정적인 사용자 경험을 느끼는지, 어떠한 조건을 더 선호하는지에 대해 알아보았다. 구체적으로, 참가자들은 4가지 종류의 휴대전화 프로토타입(청각적 피드백이 없는 무음 조건, 사람의 목소리를 통해 청각적 피드백을 제시하는 조건, 기계음을 통해 청각적 피드백을 제시하는 조건, 기계음과 사람의 목소리를 모두 제공하는 혼합음 조건)을 경험한 후, 그에 대한 경험적 사용성(재미, 흥미, 불쾌감), 유희적 측면(HQ) 그리고 선호도를 평정하였다. 결과적으로, 사용자들은 사람의 목소리를 통해 정서를 표현하는 조건의 휴대전화에 대해 청각적 피드백을 제공하지 않는 휴대전화나 기계음을 통해 청각적 피드백을 제시하는 휴대전화와 비교해서 상대적으로 높은 지각적 재미와 유희(HQ)를 느끼는 것으로 드러났다. 하지만 선호도는 다른 조건에 비해 낮은 수준의 평정치를 보이는 것으로 나타났다.

The human embryonic-lethal abnormal vision-like protein, HuR, stabilizes mRNA containing adenine- and uridine- rich elements in their 3’untranslated region. Because cyclooxygenase-2 (COX-2) mRNA is a cellular transcript that contains an adenine- and uridine-rich element, it can be regulated by the HuR protein. In this study, we examined the relationship between COX-2, HuR, MVD, and the clinicopathological parameters. Nineteen out of 43 cases of HNSCC showed high level of COX-2expression, and 68% of these patients showed high COX-2 immuno-reactivity indicating the strong expression of the cytoplasmic HuR protein. Also, MVD expression in the cases with high COX-2 expression was higher than in the cases with low COX-2 expression. These results suggest a strong correlation between the overexpression of cytoplasmic HuR and COX-2 expression in HNSCC, and that COX-2 is associated with MVD in HNSCC. In conclusion, COX-2 regulated by cytoplasmic HuR may be a good tumor angiogenic factor in HNSCC.

Numerous bone cell culture models have been presented by the development of isolation and culturing techniques of cells. The culture of osteoblast-like cells of human origin with a specific osteoblastic phenotype has become an important experimental model in bone biology. Recently, it has become increasingly popular to utilize bone marrow cultures because these cultures are therefore thought to represent earlier stages of the osteoblast differentiation pathway. There is no report about culturing normal human osteoblast from oral and maxillofacial area. Primary cultured cells from oral and maxillofacial cancellous bone were analyzed by morphologic features, total DNA contents, ALP, osteocalcin and von Kossa staining positivity. The purpose of this study were to culture the cell population from oral and maxillofacial cancellous bone and to analyze the phenotypic expression of cultured normal human osteoblast by the bone marrow isolation technique. Growth curve of NHost showed about 45hrs of doubling time and about 70μ g/well of total DNA content. NHost showed spindle shaped cytoplasm with ovoid nucleus under preconfluency and after cellular differentiation, they formed irregular numerous nodules from stratified cellular layers under D medium. ALP activity was about 2 folds higher under control medium with 10nM 1,25(OH)2D3 than that under control medium. Osteocalcin expression was about seven folds higher under control medium with 100nM 1,25(OH)2D3 than that under control medium. Scattered mineralized nodules stained by von Kossa method were seen on the cellular layer under D medium. It suggested that NHost might be established from oral and maxillofacial area by characteristic cellular shape, ALP activity, osteocalcin expression and numerous mineralized nodules.