Background : Panax ginseng C. A. Meyer is a medicinal plant corresponding to 'raw materials for food use' in Korean food standards codex, and it is known that it has the same origin although it is classified into wild ginseng, cultured ginseng and red ginseng. It varies morphologically, molecularly, depending on the cultivation or habitat environment. In this study, we carried out experiments for the morphological differentiation of cultured ginseng (Hongcheon, Gunsan, Miryang) and adventitious roots culture of wild mountain ginseng (by company). Methods and Results : Morphological observations were made with resin sectioning method (RSM). The test method was sampled, preprocessed, embedded, segmented, slid and then observed with an optical microscope. The epidermis, cortex and vascular tissues were identified in the inner cross section of adventitious roots culture of wild mountain ginseng. Phloem was formed at the center, and xylem was formed at the side. In cultured ginseng, the organs of the vascular tissues were differentiated into two, whereas in the adventitious roots culture of wild mountain ginseng, one vascular tissue was formed due to the thin adventitious. Conclusion : Plant resources have the diversity of using the same or different origins depending on their distribution. The morphological differentiation method of ginseng and adventitious roots culture of wild mountain ginseng is expected to be based on domestic fair trade to prevent discrimination.

Background : The interest in paddy-converted fields to be used as an alternative new farming area for the stable ginseng production is increasing, and made up -32% of the total ginseng cultivation area in Korea in 2014. Therefore, this study examined how soil types and fertilizer regimes influence fatty acid (FA) and vitamin E (Vit-E) variation in 6-years-old ginseng root. Methods and Results : Ginseng variety “Jagyeongjong” was cultivated upland and paddy-converted field, and three organic fertilizers were applied to both fields, at 0, 1, 2, and 4 tons per 1,000 ㎡, prior to the ginseng seedling transplantation. The 37 FAs and 8 Vit-E were analyzed with GC-FID. We observed significant variation in both FA and Vit-E contents owing to the type and quantity of organic fertilizer used in each soil type during cultivation. Unsaturated FAs were approximately 2.7-fold higher in ginseng than in saturated FAs. Linoleic, palmitic, and oleic acids were the most abundant FAs detected in ginseng roots. Additionally, α-tocopherol was the major Vit-E detected. In particular, the increased application of rice straw compost or food waste fertilizer elevated the quantity of nutritionally desirable FAs and bioactive Vit-E in ginseng root. Partial least square-discriminant analysis (PLS-DA) score plots showed that soil type might be the main cause of differences in FA and Vit-E levels in ginseng. Specifically, the PLS-DA model indicated that palmitic acid is a suitable FA marker in determining whether ginseng plants were grown in a paddy-converted field or an upland field. Moreover, linoleic acid levels were highly correlated with α-linolenic acid (r = 0.8374) according to Pearson's correlations and hierarchical clustering analysis. Conclusion : This study investigated how soil type, as well as organic fertilizer type and amount, altered the FA and Vit-E profiles in ginseng roots. Therefore, these preliminary data should be beneficial to ginseng farmers and any industries involved in the production of high quality and nutritional ginseng products.

Background : Recently, there is a urgent demand for development of new varieties with enhanced resistance to various biotic/abiotic stresses. In order to develop ginseng varieties with such traits, systematic breeding program and comprehensive field studies are prerequisite. Methods and Results : ‘Jinwon' seeds were collected in 2003 from the farmer field of Pocheon. Physiological investigation and propagation were conducted from 2008 to 2010. It was given the name 'Eumseong No. 11 through the observed yield trial from 2011 to 2013 and local adaptability was carried out from 2014 to 2017. All phenotypes including agronomic characteristics, seed yield, and physiological response to biotic/abiotic stresses were investigated according to the ginseng GAP and UPOV guidelines. Red leaf and red berry at maturing stage were observed. The time of emergence, flowering and berry maturity of the ‘Jinwon’ were faster than those of ‘Chunpoong’. Stem length of ‘Jinwon’ was longer than that of ‘Chunpoong’, whereas stem diameter was thicker than that of ‘Chunpoong’. Main root length was similar but main root diameter is thicker than that of ‘Chunpoong’. The yield performance of this variety was 576 ㎏/10 a in local adaptability test for two years, which is 9% higher than that of ‘Chunpoong’. ‘Jinwon’ showed strong resistance at high temperatures. Conclusion : Our study demonstrated that ‘Jinwon’ is an ideal variety with heavier root weight and enhanced stress resistance and contribute will enhance biotic/abiotic stress resistance and increase the farmers' income.

Background : The several studies on the characteristics of Korean ginseng cultivars and breeding lines have already been carried out the level of molecular classification analysis in Korea. In spite of where Geumsan is a representative place of Korean ginseng, Geumsan native species (breeding lines) have not yet been carry out analysis of morphological, genetic characteristics and relationship. We have plan to carry out morphological, genetic characteristics and relationship for Geumsan native species, breeding lines. Furthermore, We could be used diverse genetic resources for Ginseng breeding. Methods and Results : In this study, a total of 71 breeding lines and variety (GS97-1 - Geumwon) consisting of native ginseng collections from Geumsan was analyzed to identify for Korean ginseng variety respectively, and clustered for the selection of Geumsan native ginseng in Korea using DNA markers. We collected 71 Ginseng breeding lines from Geumsan. Analyses of the genetic characteristics of the collection were conducted for extraction gDNA using sprout. We were measured DNA concentration using QIAxpert (QIAGEN). Each DNA sample was quantified at the final DNA concentration of 5 ng/㎖ using sterilized distilled water. Korean ginseng 14 variety and 57 Ginseng breeding lines from Geumsan could be identified polymorphism using the selected 6 primer (MFGp183, MFGp130, MFGp110_E, UFGp163, MFGp108 and UFGp156). Conclusion : These finding could be used for morphological and genetic characteristics for produced native ginseng in Geumsan area. Futhermore, we could be used diverse genetic resources for Ginseng breeding.

Background : For ginseng breed development, mostly the pure-line separation method that registers excellent varieties after performing the regional adaptation test with the varieties selected through the preliminary and main productivity tests on selected excellent individuals cultivated in farms or excellent lineages for growing from collected lineages is used. Recently, the quality of ginseng is degrading while the yield decreases by 15 - 20% due to the high-temperature injury, the physiological disorder and/or the diseases caused by the climate warming so that development of disaster tolerant varieties strong against high temperatures, salts and diseases are demanded. For such reason, three lineages showing excellent overground and underground growing were selected from the gene resources collected from 1997 to 2000. And the productivity test has been conducted with these lineages having Chunpoong and Yunpoong, the existing varieties as the reference varieties. For the lineages excellent in growing, the variety registration will be performed after the regional adaptation test. Methods and Results : The results of productivity tests on the 3 lineages (GS98-3-4, GS00-58 and GS97-69) selected for excellent growing while having Chunpoong and Yunpoong as the reference varieties show that on the 2-years-old dormant roots, all of the selected 3 lineages are better in growing compared to the reference varieties. Especially, GS98-3-4 shows excellent leaf lengths and stem lengths while GS97-69 shows excellent leaf widths. On the overground growing of 3-years-old ones, GS00-58 and GS97-69 lineages are exceedingly more excellent compared to the reference varieties while the selected 3 lineages showing superb underground growing. Especially, GS98-3-4 lineage shows great root weights and diameters. Conclusion : According to the productivity tests on the 3 lineages selected for excellent growing, the selected lineages are excellent in both overground and underground growing compared to the reference varieties of Chunpoong and Yunpoong. On the 2-years-old ginsengs, GS98-3-4 shows excellent overground growing while GS97-69 shows excellent underground growing. On the 3-years-old ginsengs, GS00-58 and GS97-69 show excellent overground growing while GS98-3-4 shows excellent underground growing.

Background : Ginseng root rot is a devastating disease caused by the fungus, Ilyonectria mors-panacis that generally attacks younger roots (-2 years), leading to defects in root quality, ginsenoside accumulation and also life cycle of the plant. Hence, there is an indispensable need to develop strategies resulting in tolerance against ginseng root rot. The protective role of silicon during pathogen infestation is well documented in other plant systems and a previous study demonstrated that silica nanoparticles are absorbed and accumulated more than the bulk silica in maize. However, the role of silica in ginseng-root rot pathosystem is unknown. Methods and Results : In the present study, we evaluated the effect of silica nanoparticles (N-SiO2) in Panax ginseng during I. mors-panacis infection. Long term analysis (30 dpi) revealed a striking 50% reduction in disease severity index upon 1 mM and 2 mM treatment of N-SiO2. However, N-SiO2 did not have any direct antifungal activity against I. mors-panacis. Candidate genes and metabolites based approach revealed jasmonic acid (JA) mediated sterol accumulation and incresed ginsenside biosyntesis as the key transcriptional reprogramming events orchestrated by N-SiO2 during the fungal infection. Conclusion : In a nut shell, N-SiO2 administration induces transcriptional reprogramming in ginseng roots, leading to increased phytosterol and ginsenosides synthesis resulting in enhanced tolerance against I. mors-panacis.

Background : This study was carried out to investigate the differences of growth characteristics, photosynthetic rate and ginsenoside content of Panax ginseng C. A. Meyer according to the covering materials during house cultivation. Also the results of this study will be used as basic data for the standardization of ginseng house cultivation. Methods and Results : This experiment site was conducted from March to October, 2017 in the house located in Jinan - gun, Jeollabuk-do. The ginseng cultivar used in the experiment was a violet stem variant of 4-years-old. There are three kinds of covering materials used in the experiments: polyethylene (PE) film, scattering film, and blue-white film. PE film and the scattering film were first coated with 85% of light shielding net after the first installation, and then secondly coated with 75% and 30% of the light shielding net respectively in the middle of May when the house temperature reached 25℃. The blue-white film was cultivated after the initial installation without further treatment. The light transmittance of each covering materials were in the order of scattering film (14.5 ± 1.5%), blue-white film (10.0 ± 1.0%) and PE film (6.7 ± 1.0%). The average photosynthetic rate of each treatment was in the order of scattering film (2.94 μmol CO2/㎡/s), blue film (2.71 μmol CO2/㎡/s) and PE film (2.34 μmol CO2/㎡/s). Aboveground growth characteristics of ginseng were good in a scattering film and blue-white film. Root weights were found in the order of scattering film (47.1 g/plant) > blue-green film (41.3 g/plant) > PE film (37.6 g/plant). Conclusion : In order to select proper covering materials for ginseng house cultivation, the growth and photosynthetic characteristics of each treatment were compared with each other in order of scattering film, blue-white film and PE film. The photosynthetic rate of each treatment was measured according to the growing season. PE film was relatively low during the entire growth period. In the case of scattering film and blue-white film, the rate of photosynthesis was higher in the scattering film with high light transmittance until May, but showed a similar tendency after July. As a result, the difference in the growth characteristics of ginseng is thought to be due to differences in photosynthesis and assimilation according to the coating materials.

Background : This study was carried out to secure technology for stable production of ginseng by developing technology to reduce the high temperature in response to climate change. Methods and Results : Polyethylene film (PE film), scattering film and blue-white double-sided film (85% shading rate) were tested for ginseng rain-shielding materials. The shading materials used were a black shading net (90% shading rate) in the first, and an aluminum screen (40% shading rate) in the second. The first shading materials were installed on April 10 with PE film + black light shielding net, and scattering film + black light shielding net. As the treatment for high temperature injury relief, an aluminum screen was installed under a scattering film + black light shielding net and a blue-white double-sided film at a high temperature. The amount of transmitted light was 12.9% at scattered film, 14.5% at double sided film, and 12.5% at PE film when the primary rain-shielding material was covered. In secondary shading, scattering film + aluminum screen and blue-white double-sided film + aluminum showed 8.6% and 7.6% of light transmitting, respectively. In the case of temperature, the average temperature was lower than the outside temperature at the scattering film + aluminum screen, clear double sided film + aluminum screen and PE film recovery. The differences among the cultivars were significant in plant height, stem height, stem diameter, leaf length, leaf width and leaf area. There were significant differences in the plant height, stem height, leaf length, and leaf width between treatments, but there was no interaction effect between varieties and treatments. The interactions between treatments were found only in the number of brach root. Conclusion : High temperature damage was influenced by the amount of irradiation in the facility rather than the temperature, which was presumed to be due to the accelerated aging of the ginseng leaf as the light transmittance increased.

Background : This experiment was conducted to study the characteristics of Korean ginseng (Panax ginseng C. A. Meyer) growth by LED artificial light source in the plastic house using multi-stage cultivation facility and artificial soil. Methods and Results : Seedlings of Korean ginseng cultivar cultivated from 6:00 am to 6:00 pm by adjusting the ratio of 1 : 1, 2 : 1 and 3 : 1 for red light and blue light for the LED light quality test. Controls were tested by treating the white light (fluorescent lamps) for the same time. The average temperature inside the plastic house remained above 25℃, then below 25℃ after mid-August and below 20℃ after mid-September. The temperature near the roots of the artificial soils was higher than 26℃ in late July and early August and lowered to 20℃ or lower in all treatments in September. The pH of the soil was in the standard range, but the EC value was 2.89 in the soil before the test and 5.83 in the white light treatment, higher than the standard range of 1.0. The light intensities were 95.3 μmol and 94.9 μmol in the 3 : 1 and 2 : 1 ratios of red and blue light, respectively. The photosynthetic rate was 1.72 μmole CO2/㎡/s in 3 : 1 treatment and 1.9 times higher than white light treatment. As the red light ratio increased, the light amount and photosynthetic rate increased. Growth characteristics of aerial parts (plant height, leaf length etc.) by LED irradiation were longer than that of white light treatment in the 2 : 1 or 3 : 1 treatment of red light and blue light. The root length was the longest at 13.7 ㎝ in the 3 : 1 treatment of red and blue light, taproot length were 6.9 ㎝ and 6.6 ㎝ in the 2 : 1 treatment and 3 : 1 treatment, respectively, longer than 4.3 ㎝ in white light. Root fresh weight was the heaviest (3.6 g/plant) in 3 : 1 treatment for red and green lights. Conclusion : It was confirmed that the rate of photosynthesis of Korean ginseng was higher than that of white light treatment and the growth characteristics of aerial and root parts were excellent at the ratio of 3 : 1 of LED red light and blue light in plastic house using multi-stage cultivation facility and artificial soil.

Background : This study was conducted to investigate the effect of inorganic compounds (germanium etc) on the characteristics of growth and ginsenoside contents in Korean ginseng (Panax ginseng C. A. Meyer) cultivated in plastic houses. Methods and Results : The experiments were performed in the Jinan (elevation: 300 meters above sea level). Seedlings (n = 72 per 3.3㎡) of Korean ginseng cultivar were planted on March 23, 2015. Shading material of plastic house was blue-white vinyl. From May to September of each year, 500 and 1,000 times diluted solution of germanium and silicate and these mixed solutions were drenched once a month. The chemical properties of the soil are as follows : pH, EC, organic content, and P2O5 of the soil were within the proper range, and the Mg content of the Ex-cations was lower than the standard value in 3 treatments, including 500 times solution of silicate. The rate of photosynthesis in Korean ginseng leaves were 2.2 - 2.5 μmole CO2/㎡/s in germanium and silicate treatment than in the non-treatment (1.5 μmole CO2/㎡/s). In the mixed treatment of silicate and germanium, the growth characteristics of the aerial parts (leaf and stem) of the Korean ginseng plants tended to be higher and longer than the other treatments. In a mixed germanium and silicate treatment, the root length was 33.3 ㎝, diameter was 23.5 ㎜, and the weight was the best at 53.8 g. The ginsenoside content was 16.8 ㎎/g in germanium treatment, 16.3 ㎎/g in germanium + silicate treatment. These were 37% and 33% higher than the control, respectively. The incidence of disease such as Alternaria panax was less in the inorganic treatment than in the non-treatment, and the incidence of disease was significantly decreased in the treatment with germanium + silicate. Conclusion : By soil drench of inorganic ingredients in Korean ginseng plastic house cultivation, the growth of the aerial and root parts in ginseng were more improved than non-treatment and ginsenoside content was increased and decreased the incidence of disease.

Background : The purpose of this study was to establish the optimum organic compost composition and fertilizer application for nutrient management during the cultivation of Korean ginseng (Panax ginseng C. A. Meyer) in the greenhouse. Methods and Results : Wood chips, popped rice hulls, and mixed expeller cake were mixed with certain contents, fermented microorganisms were added, and fermented for about 100 days to produce organic composts. Organic compost was applied to the greenhouse soils at a rate of 1,500 – 3,000 ㎏/10 a, and the Korean ginseng seedlings were planted (2015) and cultivated. The growth and quality characteristics of 4-years-old Korean ginseng were investigated as follows. In all treatments, the emergence date were April 17, and the emergence rate were 70 - 74%. Soil chemical properties such as pH (6.7 - 6.8) and trace element Ca (7.9 - 9.2 cmol+/㎏) were higher than the standard values, and EC, P2O5 and OM were appropriate. Disease incidence rate were less than 0.2% in the control (treatments of livestock manure compost 1,500 ㎏/10 a) and treatment 3 (wood chips 60% + popped rice hulls 40%), but there were no disease in other treatments. Plant growth such as plant height, leaf length, and stem diameter were superior to treatment 1 (wood chips 20% + popped rice hulls 80%), and growth of underground part such as root length were excellent in treatment 1 as a whole. In particular, in the application of 1,500 - 2,000 ㎏ of treatment 1, root weight (39.9 g, 42.3 g/plant, respectively) were superior to the other treatments. In general, as woodchip content and usage amount increase, root weight tended to decrease. Conclusion : It is considered appropriate to apply 1,500 - 2,000 ㎏/10 a of fermented compost of organic material which is mixed with wood chips (20%) and popped rice hulls (80%) with mixed expeller cake when growing Korean ginseng in the plastic house covered with blue-white vinyl.

Background : Currently sprout ginsengs are produced in extensive variations of cultivation times, methods and specifications that are different in each farm in Korea. Also, regardless the effective ingredient content of ginseng, overgrown sprout ginseng are distributed so that the quality standardization of sprout ginseng is urgently required. The sprout ginseng market is only a segment of the Korean ginseng market still. However, If development of processed products utilizing the functionalities is connected, the market may exponentially grow. Also, if the use of sprout ginseng is extended to productions of drinks, cosmetics, facial masks and others in the cosmetics and health functional food industries, the export competitiveness to the global market is expected to increase. Therefore, this study aims the quality standardization of sprout ginseng to secure the market competitiveness in Korea and overseas by examining the property change following the cultivation phases. Methods and Results : For the overground growing in green houses, the sprout ginsengs planted in early April and early June show to be better in growing than the ones planted in early August. For the underground growing, while no significance is found among the cultivation periods, the sprout ginsengs planted in early June are excellent in terms of the root length and the weight of overground portion. Also, according to the examination on the stem properties of 2-year grown sprout ginsengs in each cultivation time, a trend that, as the growing period is longer from 25 days to 35 days, 45 days and 95 days, the hardness, gumminess, chewingness and springiness of stem are higher. And, on the hardness, gumminess, chewingness and springiness of leaf, while no statistically significance is found in the growing periods from 25 days to 45 days, the 95-day sprout ginsengs show high figures referring that the leaves becomes tougher. Conclusion : In case of cultivating sprout ginsengs in a green house, cultivating in April - June is advantageous for the overground and underground growing. And, it is determined that cultivating sprout ginsengs for 35 - 45 days produces leaves and stems with the most excellent chewing textures felt by consumers when chewing.

Background : The first planted field is decreasing due to replant failure and climate change. As a result, there is an increasing number of cases where new soil is covered in agricultural land or clearing forest to cultivate ginsengs. In this case, it is essential to improve the chemical properties of the soil before ginseng site management. This study was carried out to investigate growth, quality of ginseng and soil chemical properties to set the concentrations of nitrogen. Methods and Results : 0, 1, 4, 8, 16, 32 ㎏/10 a of urea were each treated at 3.3 ㎡ of the field and ginseng cultivar 'Gumpoong' was transplanted. Growth characteristics were investigated by growth period and soil chemical properties were investigated every 3 months. In 2-years-old ginseng, the root weight was the highest at 4 ㎏ treatment group of nitrogen while it showed the rate of increase in root weight is increased at 1 ㎏ treatment of nitrogen in 3-years-old ginseng. Physiological disorder and root rot symptom are increased at 8 ㎏ treatment group of nitrogen in 2- and 3-years-old ginseng. In case of soil properties, EC and Nitrate-N concentration exceeded 0.5 dS/m and 50 ㎎/㎏ respectively from 16 ㎏ treatment group of nitrogen after 14 months. The saponin content tended to decrease with increasing nitrogen treatment concentration at the 2-years-old ginseng. In the case of 3-years-old, the highest value was 0.88% at 1 ㎏ nitrogen treatment group. Conclusion : These results indicate that the application levels of nitrogen influence growth, quality of ginseng and soil chemical properties. These data can be used to set the concentrations of nitrogen when new soil is covered in agricultural land or clearing forest to cultivate ginseng.

Background : This study researches on the microclimate, photosynthesis and growth characteristics for the development of shading materials proper for the wide and inclined ginseng cultivation facility which can respond to climate change and save labor. Methods and Results : The wide shading facilities were installed on the area of 1,000 ㎡ in 2014 and 4 facilities were installed on the test ginseng cultivation area. On Mar. 29, 2017, 2 blue shading nets [with the sun blocking rate of 85% (200 g/㎡) and 90% (220 g/㎡)] were installed each for 4 facilities. On June 26, 2017, the aluminum screen and black shading net (with the sun blocking rate of 40% each) were installed during the period of high temperature (30℃ or higher) for each facility. The maximum light intensity under the shading facility was high with blue shading net 85% and PE black shading net 40%, or blue shading net 85% and aluminum screen 40%, higher than other treatments. They were higher by 4.8 - 7.3%, 5.3 - 7.8% each in July and August. Among the coating materials for reducing the high temperature, the aluminum screen coating had less water leakage in early July, late July, mid-August and late August when the precipitation was more than 100 ㎜. The death of the aerial part of ginseng occurred less until October. The growth of the aerial part of 4-year ginseng was better in blue shading net 85% and PE black shading net 40%, blue shading net 85% and aluminum screen 40% or blue shading net 90% and PE black shading net 40% than in blue shading net 90% and aluminum screen 40% The photosynthesis rate was the highest in June with 3.67 μmol CO2/㎡/s under the blue shading net 90% and aluminum screen 40% and with 3.55 μmol CO2/㎡/s under blue shading net 85% and PE black shading net 40% for K-1. As for the land races ginseng, it was the highest with 3.55 μmol CO2/㎡/s under the blue shading net 85% and PE black shading net 40%. For the growth of the underground part of the 4-year ginseng, the blue shading net 85% + PE black shading net 40% or the blue shading net 85% + aluminum screen 40% was the best with respect to the growth of the ground part of the ginseng such as the length of root, the length of main root, diameter of root and weight of root than other treatment. Conclusion : Best coating materials for the wide shading facilities are the blue shading net 85% and aluminum screen 40%.

Background : Replant failure of ginseng is caused by soil-born pathogens causing root rot such as Cylindrocarpon destructans and Fusarium solani. Dazomet are widely used as soil fumigant to solve soilborne problems, and the degradation intermediates are toxic to nematodes, fungi, bacteria, insects and weeds. Methods and Results : The effects of cultivation of green manure crop, maize before and after soil fumigation on the control of ginseng root rot disease were compared using soil of field where 6-years-old ginseng was harvested. Fumigant (dazomet) were used for soil fumigation in May and September, respectively. Maize was grown for soil management before and after soil fumigation. Maize cultivation after May fumigation was delayed the sowing day by 15 days, and the fresh and dry weight decreased significantly. Maize cultivation after May fumigation increased pH but decreased EC, NO3, P2O5, and K significantly. Maize cultivation after May fumigation decreased fungi population and ratio of fungi and bacteria. Growth of 2-years-old ginseng was improved and the incidence of ginseng root rot was significantly decreased by maize cultivation after May fumigation. After harvesting 2-years-old ginseng, the population of Cylindrocarpon destructans was not different among treatments, but Fusarium solani showed a significant increase in September fumigation after maize cultivation. Conclusion : Maize cultivation after soil fumigation was effective in inhibiting ginseng root rot by improvement of mineral composition and microorganism in soils.

Background: The cultivation of ginseng (Panax ginseng C. A. Meyer) in greenhouses could reduce the use of pesticides and result in higher yield; however, construction costs are problematic. The adaptation of direct-sowing culture in greenhouses could reduce the cost of ginseng production. Methods and Results: To improve seedling establishment in direct-sowing culture, effects of sowing density (SD), number of seeds sown per hole (SN), and thinning (TH) treatment on the root yield were investigated after 3 years of seeding. The emergence rate was significantly influenced by SD, but not by SN or TH. Damping-off and rusty roots increased with an increase in SN with diminishing effects of SN on seedling establishment. Root weight and diameter were affected by SD, SN, and TH, however, there were no statistical significances. The total number of roots harvested per unit area increased with increasing SD and SN, and the weight of roots was affected by SD, but not by SN or TH. Conclusions: Multi-seed sowing per hole and/or thinning might not be an efficient method for the direct-sowing culture of ginseng. The SD for direct seeding culture in greenhouses should be approximately 33 - 42 seeds/㎡ for an optimum yield of 3-year-old ginseng.

The present study was carried out to investigate the physicochemical properties of hot water extracts of red ginseng powder prepared by two-stage hot air drying method using steamed ginseng and steaming liquid for 2.5 h under high-temperature and high-pressure autoclave condition. The total polyphenols, total flavonoids, total sugar, acid polysaccharides and crude saponin in hot water extracts from red ginseng powder were analyzed and determined, and the flavor components of ginseng were measured using color difference meter and an electronic tongue. The total polyphenol, total flavonoid, total polysaccharide, and acid polysaccharide of the red ginseng hot water extract obtained by autoclaving (ARG) were 9.06 mg GAE/g, 3.38 mg NE/g, 35.22 g/100 g, and 10.90 g/100 g, respectively. The final contents of the total polyphenols, total flavonoids, crude saponin were higher than those determined using other red ginseng methods; the time required for steamed red ginseng production reduced. The total ginsenoside content of ginseng including Rb1 was 10.69 mg/g, which is the lowest ARG. The processing conditions affected the conversion to ginsenosides unique to red ginseng. Red ginseng and steaming liquid obtained from the autoclave are expected to be in need for non-food materials and products as well as foods by improving the flavor components through conversion of red ginseng components into low molecular weight.

Background: This study was conducted to acquire basic information on the phenotypic and genotypic characteristics of the germplasm of Panax ginseng C. A. Meyer collected from China and Korea, and identify the variations that can be utilized in ginseng breeding programs. Methods and Results: Quantitative parameters were evaluated, and used to compare and analyze on genetic polymorphisms in the germplasm. The genetic characteristics and classifications were compared and analyzed for each character. Stem length followed a normal frequency distribution ranging from 15.5 ㎝ to 40.5 ㎝, with showing approximately 40% having a stem length of 20 - 30 ㎜. Stem diameters ranged from 2.7 ㎜ to 11.3 ㎜. Stem number per plant ranged from 1 to 3; approximately 50% had a single stem, and 45% had two stems. A non-normal frequency distribution was observed for petiole number, with approximately 60% of the germplasm having 3 - 5 petioles. Petiole length exhibited a normal frequency distribution, raging from 4.5 to 10.6. Petiole angle in the germplasm ranged from 28° to 89° and seedstalk length ranged from 5.6 ㎝ to 27.3 ㎝. Conclusions: The genetic polymorphisms identified by complete linkage clustering based on the quantitative characteristics of Panax ginseng C. A. Meyer collected from Korea and China were classified to 6 groups, namely I, II, III, IV, V, and VI with frequencies of 6.7%, 20.0%, 31.7%, 8.3%, 6.7%, and 26.7%, respectively.

재배지 부족과 기후변화로 인하여 인삼재배의 생산량이 적 어지고 있어 추가적인 방법이 필요하다. 따라서 기존에는 환경 산업 및 식품포장, 가공에 활용되고 있는 파인버블을 우리나라 대표 작물인 인삼재배에 적용하여 변화되는 생리적, 형태적 특성을 분석하였다. 2년 근 인삼에 파인버블수를 적용한 결과 Table 2에서 보듯이 줄기 길이와 잎이 증가되었고 뿌리에서는 주근이 커지면서 무게가 증가되었다. 특히 잎이 커지면서 뿌리의 무게가 증가됨을 보이고 있는데 이는 총 엽록소 함량을 확인한 결과 높게 나와 광합성 효율 증대와 연계되었다고 판단된다. 본 연구결과에서는 파인버블수를 사용한 인삼 재배시 인삼의 성장 저해가 나타나지 않았음을 확인하였으며, 생리적 특성 및 세분화된 결과를 통해 인삼의 전체적인 생육이 약 10%에서 15% 생장이 증가되는 것을 확인할 수가 있다. 따라서 인삼 재배시 파인버블수를 적용함으로써 인삼이 생육 증대가 이루어지고 있다는 것을 의미할 수 있다. 추후 파인 버블수를 적용된 3-5년 근의 고년 근 인삼에서도 특징을 유지하는지 추가적인 연구가 필요하다고 사료된다.