Oral squamous cell carcinoma (SCC) is one of the leading causes of cancer mortality worldwide. It is generally thought that adjuvant chemotherapy provides modest prolongation of survival in various carcinoma. Docetaxel (Taxotere, TXT) play a significant role in the treatment of various solid tumors of epithelial origin. CsA (immunosuppressive drug) was widely used as adjunct for the treatment of cancer. Thus, it is important to pursue the apoptosis of IHOK and oral SCC induced by TXT combined with CsA related to the pathogenesis of oral SCC. But TXT combined CsA effect on IHOK and oral SCC remains unclear. After cultured IHOK and HN 22 oral squamous cell carcinoma cell line treated by 10 nM TXT and 1 μM, and caspase inhobitor, respectively, apoptosis index, cytochrome c and caspase-3 -8, -9 mRNA expression by RT-PCR, and procaspase-3 protein amount by immunoslot blotting was prepared. The purpose of this study were to examine the TXT-induced apoptosis pathway via caspase activation by CsA enhancement, and to apply these results to an effective therapeutic treatment plan for oral SCC by TXT combined CsA . 10 nM TXT showed about 60%, 55% celluar apoptosis of IHOK and HN 22, cell line, respectively, while CsA alone did not induce apoptosis in IHOK and HN 22 cell line. 1 μM CsA combined with 10 nM TXT increased apoptosis in IHOK and HN 22 cell line through caspase-3 and cytochrome c mRNA expression, while could not effect on caspase-8 and -9. Caspase inhibitor suppressed apoptosis of IHOK and HN 22 cell line induced by a combination of 1 μM CsA and 10 nM TXT. Immnoslot blotting showed procaspase-3 activation by a combination 1 μM CsA and 10 nM TXT, while caspase inhibitor inhibited activation. It suggested that a combination of CsA and TXT might induce increased apoptosis of IHOK and HN 22 oral squamous cell carcinoma cell line through caspase-3 activation. This treatment with a combination of TXT and CsA may be an effective therapeutic strategy for oral squamous cell carcinoma

Periodontal disease is a major oral disorder and comprises a group of infections that lead to inflammation of the gingiva and the destruction of periodontal tissues. PPARy plays an important role in the regulation of several metabolic pathways and has recently been implicated in inflammatory response pathways. However, its effects on periodontal inflammation have yet to be clarified. In our current study, we evaluated the anti-inflammatory effects of PPARy on periodontal disease. Human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) showed high levels of intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), matrix metalloproteinase-2 (MMP-2), and -9 (MMP-9). Moreover, these cells also showed upregulated activities for extracellular signal regulated kinase (ERK1/2), inducible nitric oxide synthase (iNOS) and cyclooxygnase-2. However, cells treated with Ad/PPARy and rosiglitazone in same culture system showed reduced ICAM-1, VCAM-1, MMP-2, -9 and COX-2. Finally, the anti-inflammatory effects of PPARy appear to be mediated via the suppression of the ERK1/2 pathway and consequent inhibition of NF-kB translocation. Our present findings thus suggest that PPARy indeed has a pivotal role in gingival inflammation and may be a putative molecular target for future therapeutic strategies to control chronic periodontal disease.

The purpose of this study was to determine the effects of heat appli˗ cation on the immune activities of the human body. To exam, further˗ more, the immune effect from the healthy volunteer(male:15, female:15) by monitoring changes of immune substances such as various leukocytes[total white blood cell(WBC), eosinophil, neutrophil, basophil, monocyte, and lymphocyte], a comparative study with warm water immersion(40.8±0.3℃) and infrared(250W) was carried out. The plasma analysis showed that the count of white blood cell, eosinophil, and neutrophil were elevated in warm water immersion- or infrared˗ stimulated group compared with control group. However, the count of basophil was decreased in both warm water immersion- and infrared-stimulated group than control group. Therefore, these results suggest that the thermostimulation improved immune activity.

본 연구의 목적은 높이맵 기반의 액체 시뮬레이션을 이용하여 인간 친화적으로 표현하기 위한 감성 컨텐츠 제작 에 있다. 액체를 인간 친화적으로 표현하는 기존 연구들은 액체 표면을 표현하는데 몇 가지 문제점이 있다. 이러한 문 제를 해결하기 위해 본 연구는 액체의 광학적 특징을 이용한 새로운 액체의 카툰 렌더링 기법을 제안한다. 본 연구 에서 제안하는 액체의 카툰 렌더링은 반사와 굴절을 독립적으로 표현한 채색 단계와 액체의 경계선과 파형선을 표현 하는 선화 단계를 거쳐 표현된다. 그 결과 인간 친화적으로 표현된 액체의 표면을 실시간으로 감성 컨텐츠로 제작할 수 있다.

Many studies propose that dysfunction of mitochondrial proton-translocating NADH-ubiquinone oxidoreductase (complex I) is associated with neurodegenerative disorders, such as Parkinson's disease and Huntington's disease. Mammalian mitochondrial proton-translocating NADH-quinone oxidoreductase (complex I) consists of at least 46 different subunits. In contrast, the NDI1 gene of Saccharomyces cerevisiae is a single subunit rotenone-insensitive NADH-quinone oxidoreductase that is located on the matrix side of the inner mitochondrial membrane. With a recombinant adeno-associated virus vector carrying the NDI1 gene (rAAV-NDI1) as the gene delivery method, we were able to attain high transduction efficiencies even in the human epithelial cervical cancer cells that are difficult to transfect by lipofection or calcium phosphate precipitation methods. Using a rAAV-NDI1, we demonstrated that the Ndi1 enzyme is successfully expressed in HeLa cells. The expressed Ndi1 enzyme was recognized to be localized in mitochondria by confocal immunofluorescence microscopic analyses and immunoblotting. Using digitonin-permeabilized cells, it was shown that the NADH oxidase activity of the NDI1-transduced HeLa cells were not affected by rotenone which is inhibitor of complex I, but was inhibited by flavone and antimycin A. The NDI1-transduced cells were able to grow in media containing rotenone. In contrast, control cells that did not receive the NDI1 gene failed to survive. In particular, in the NDI1-transduced cells, the yeast enzyme becomes integrated into the human respiratory chain. It is concluded that the NDI1 gene provides a potentially useful tool for gene therapy of mitochondrial diseases caused by complex I deficiency.

본 연구는 터널이 갖는 다양한 특성 가운데 운전자의 심리적 안정성과 쾌적성 등 경관을 구성하는 설계요인과 운전자의 감성적 요인간의 관계를 파악하여, 터널경관에 대한 평가방법을 제시하고, 이를 위하여 터널경관을 구성하는 물리적인 구성요소와 운전자의 감성인지 변화와의 관계를 파악하여 경관적 환경을 조성할 수 있는 방안을 제시하는데 연구의 목적이 있다. 터널경관에 대한 감성인지특성을 파악하기 위한 LISREL 모형을 구축한 결과 터널경관 영향요인은 터널경관 구성요소 조화에 의한 감성 이미지인 것으로 분석되었다. 이러한 감성이미지는 설계요인과 개인속성에 의하여 설명며, 설계요인의 영향이 개인속성의 영향보다 크게 나타났다. 개인 속성과 설계요인 모두의 관계는 (+)의 관계로 나타났고 "안정성"과 "변화성"간에는 서로 밀접한 상관관계가 있는 것으로 분석되었다. 이때, "안전성" 요인이 "변화성" 요인보다 경관인지도에 좀 더 영향을 미치는 것으로 분석되어, 경관 평가 시 운전자는 기본적으로 "안전성"을 중시하나 "변화성"에 대하여도 요구하고 있는 것으로 분석되었다.

This study examined the effects of red light generated from a light emitting diode (LED) upon proliferation and mitochondrial stress in human gingival fibroblasts (hGFs). Cells were exposed to LED-generated red light at a clinically relevant intensity and distance with a 610-630 nm wavelength for various times (0-48 min). At different exposure times, cells were processed for the analysis of succinate dehydrogenase (SDH) activity, proliferation, mitochondrial membrane potential (MMP) and cytotoxicity. Cell cycle progression was also investigated by flow cytometry after staining with propidium iodide. Red light exposure was found to inhibit SDH activity and DNA synthesis in hGFs in a time-dependent manner. Light exposure also reduced the MMP levels in these cells and this was closely associated with a G0/G1 arrest. In contrast, exposure of hGFs to red light for 48 min led to a dramatic loss of MMP with an attendant increase in cytotoxicity. These findings demonstrate that LED-generated red light may cause mitochondrial stress and growth inhibition in hGFs during tooth whitening therapy, depending on the length of the exposure.

Human mesenchymal stem cell (hMSCs) isolated from human adult bone marrow have self-renewal capacity and can differentiate into multiple cell types in vitro and in vivo. A number of studies have now demonstrated that MSCs can differentiate into various neuronal populations. Due to their autologous characteristics, replacement therapy using MSCs is considered to be safe and does not involve immunological complications. The basic helix-loop-helix (bHLH) transcription factor Olig2 is necessary for the specification of both oligodendrocytes and motor neurons during vertebrate embryogenesis. To develop an efficient method for inducing neuronal differentiation from MSCs, we attempted to optimize the culture conditions and combination with Olig2 gene overexpression. We observed neuron-like morphological changes in the hMSCs under these induction conditions and examined neuronal marker expression in these cells by RTPCR and immunocytochemistry. Our data demonstrate that the combination of Olig2 overexpression and neuron-specific conditioned medium facilitates the neuronal differentiation of hMSCs in vitro. These results will advance the development of an efficient stem cell-mediated cell therapy for human neurodegenerative diseases.

HCI 분야에서 사용성은 시스템의 객관적인 사용성에 초점을 둔 것에서 점차 사용자들이 시스템을 사용하면서 느끼는 주관적인 경험을 중시하는 개념으로 확장, 변화하고 있다. 오늘날 대부분의 사람들은 휴대전화를 소지하고 사용한다. 휴대전화와 같이 인간과의 상호작용 빈도가 높은 인터페이스에서 보다 긍정적인 사용자의 주관적 경험을 유발하는 것은 중요하다고 볼 수 있다. 본 연구에서는 감정을 표현하는 인터페이스가 인간에게 보다 긍정적인 사용자 경험을 유발할 것이라는 가설 하에 감정을 표현하는 인간 목소리를 통해 청각적 피드백을 제공하는 휴대전화 프로토타입(prototype)을 제작한 후, 감정을 표현하기에 적절하지 않은 기계음을 통해 청각피드백을 제공하는 휴대전화 조건과의 비교를 통해 어떠한 조건에서 사용자들이 보다 더 긍정적인 사용자 경험을 느끼는지, 어떠한 조건을 더 선호하는지에 대해 알아보았다. 구체적으로, 참가자들은 4가지 종류의 휴대전화 프로토타입(청각적 피드백이 없는 무음 조건, 사람의 목소리를 통해 청각적 피드백을 제시하는 조건, 기계음을 통해 청각적 피드백을 제시하는 조건, 기계음과 사람의 목소리를 모두 제공하는 혼합음 조건)을 경험한 후, 그에 대한 경험적 사용성(재미, 흥미, 불쾌감), 유희적 측면(HQ) 그리고 선호도를 평정하였다. 결과적으로, 사용자들은 사람의 목소리를 통해 정서를 표현하는 조건의 휴대전화에 대해 청각적 피드백을 제공하지 않는 휴대전화나 기계음을 통해 청각적 피드백을 제시하는 휴대전화와 비교해서 상대적으로 높은 지각적 재미와 유희(HQ)를 느끼는 것으로 드러났다. 하지만 선호도는 다른 조건에 비해 낮은 수준의 평정치를 보이는 것으로 나타났다.

본 연구의 목적은 무선 통신 사업자 간에 휴대폰에서 제공되는 휴대폰 벨소리 사운드의 음질차이가 있는지, 있다면 어느 요소에서 발생하는지를 조사하는 것이었다. 선두 무선 통신 사업자나 휴대폰 제조사가 가지는 브랜드의 영향 요소를 배재하고 음질의 차이가 생기는 요소들을 찾아내기 위한 일련의 실험이 계획되었다. 무선 통신 사업자, 휴대폰 제조사, 휴대폰 모델, 특정 장르, 음원 콘텐츠 제작자 등 음질의 차이가 발생 할 수 있는 요소들을 테스트 하였다. 연구결과 휴대폰을 통해 제공되는 벨소리 사운드는 무선 통신 사업자별로 음질 차이가 존재하는 것으로 조사되었다. 음질의 차이는 콘텐츠 및 휴대폰의 차이에서 발생하며, 동일휴대폰 모델의 경우에도 무선 통신 사업자 모델별로 음질의 차이가 발생하는 것을 알 수 있었다. 또한 콘텐츠를 제공하는 음원 제작사 별 음질의 차이도 있는 것으로 나타났다. 음악 장르에 대한 음질의 차이는 명확하게 나타나지 않았다. 제한된 샘플을 이용한 실험의 한계를 보완하기 위하여 휴대폰 제조업체의 사운드 전문가를 통한 인터뷰가 시행되었다. 인터뷰 결과 제품 가격의 차이에서 오는 스피커 단품 및 미디, 앰프 등 하드웨어적 차이가 음질에 영향을 끼치는 것으로 조사되었고, 부품 및 기구의 튜닝 또한 중요도를 갖는 것으로 조사되었다.

As the human mixed saliva plays important roles for the protection, regeneration, immunity, and molecular transfer/ signaling in the oral and gastro-intestinal mucosa, the salivary contents have great implications for the general health of human body. Nevertheless, the analysis method of human saliva has not been well developed up to date, because the proteins of mixed saliva are rapidly interacted with each other and easily degraded by proteolytic enzymes and microorganisms. This study aims to develop an immunoprecipitation-based high performance liquid chromatography (IP-HPLC) for the analysis of human mixed saliva. The representative IP-HPLC analyses were performed to compare among different subjects in variable general conditions. Compared to the normal control the subjects suffered from bacterial infections of gastro-intestinal enteritis, chronic periodontitis, and acute necrotizing gingivo-stomatitis showed dramatic increase of LL-37 level depending on the severity of diseases, while the subject suffered from Herpes stomatitis, a viral infection showed great increase of β-defensin 2. These data indicate that LL-37 in human mixed saliva is more responsible to the bacterial infections of gastro-intestinal enteritis, chronic periodontitis, and acute necrotizing gingivo-stomatitis, while β-defensin 2 is more responsible to the viral infection of Herpes stomatitis. This study also suggeststhat the IP-HPLC be easily applicable to the wide range of biological samples for the quantitative analysis of an objective protein.

The human embryonic-lethal abnormal vision-like protein, HuR, stabilizes mRNA containing adenine- and uridine- rich elements in their 3’untranslated region. Because cyclooxygenase-2 (COX-2) mRNA is a cellular transcript that contains an adenine- and uridine-rich element, it can be regulated by the HuR protein. In this study, we examined the relationship between COX-2, HuR, MVD, and the clinicopathological parameters. Nineteen out of 43 cases of HNSCC showed high level of COX-2expression, and 68% of these patients showed high COX-2 immuno-reactivity indicating the strong expression of the cytoplasmic HuR protein. Also, MVD expression in the cases with high COX-2 expression was higher than in the cases with low COX-2 expression. These results suggest a strong correlation between the overexpression of cytoplasmic HuR and COX-2 expression in HNSCC, and that COX-2 is associated with MVD in HNSCC. In conclusion, COX-2 regulated by cytoplasmic HuR may be a good tumor angiogenic factor in HNSCC.

본 연구는 역량기반 인적자원관리와 역량개발 프로세스 및 인적자원관리 성과에 대한 기존의 연구와 실제 사례를 고찰한 후, 인적자원의 전략적 활용이 특히 중시되는 해운․항만 물류기업을 대상으로 이들의 관계를 실증분석하였다. 역량기반 인적자원관리 활동을 통해 조직내 인적자원관리 성과가 향상될 것이라는 관점에서 역량기반 인적자원관리와 인적자원관리 성과와의 관계를 규명하고, 이들의 관계에 대해 역량기반 인적자원관리 활동과의 전략적 연계의 정도를 나타내는 핵심역량

Cardiovascular diseases (CVDs) are one of the most cause of death around the world and fields of interest for cardiac stem cells. Also, current use of terminally differentiated adult cardiomyocytes for CVDs has limited regenerative capacity therefore any significant cell loss may result in the development of progressive heart failure. Human embryonic stem cells (hESCs) derived from blastocyst‐stage embryos spontaneously have ability to differentiate via embryo‐like aggregates (endoderm, ectoderm and mesoderm) in vitro into various cell types including cardiomyocyte. However, most effective molecule or optimized condition which can induce cardiac differentiation of hESCs is rarely studied. In this study, we developed both spontaneous and inductive cardiomyocyte‐like cells differentiation from hESCs by treatment of induced‐factors, 5‐azacytidine, BMP‐4 and cardiogenol C. On the one hand, spontaneous and inductive cardiomyocyte‐like cells showed that cardiac markers are expressed for further analysis by RT‐PCR and immunocytochemistry. Interestingly, BMP‐4 greatly improved mogeneous population of the cardiomyocyte‐like cells from hESCs CHA15 and H09. In conclusion, we verified that spontaneously differentiated cells showed cardiac specific markers which characterize cardiac cells, treated extrinsic factors can manage cellular signals and found that hESCs can undergo differentiation into cardiomyocytes better than spontaneous group. This finding offers an insight into the inductive factor of differentiated cardiomyocytes and provides some helpful information that may offer the potential of cardiomyocytes derived from hESCs using extrinsic factors.

Cordycepin (3’-deoxyadenosin), a polyadenylation specific inhibitor, is the main functional component in Cordyceps militaris which is one of the top three famous traditional Chinese medicine. It has been shown to possess many pharmacological activities including immunologically stimulating, anti-cancer, anti-bacterial, and anti-virus, anti-infection effects. However, its anti-cancer molecular mechanisms are poorly understood. In this study, the apoptotic effects by cordycepin were investigates in human leukemia cells. Treatment of cordycepin significantly inhibited cells growth in a concentrationdependent manner by inducing apoptosis, as evidenced by morphological change and apoptotic cell death such as formation of apoptotic bodies, DNA fragmentation and increased populations of sub-G1. Induction of apoptosis by cordycepin was associated with modulation of Bcl-2 and inhibitor of apoptosis proteins (IAP) family expression. Cordycepin also increased reactive oxygen species (ROS) generation, activation of casepase-3, caspase-8, caspase-9, cleavage of poly(ADP-ribose) polymerase (PARP), β-catenin and phospholipase C (PLC)-γ1 protein. The quenching of ROS generation by N-acetyl-L-cysteine administration, a scavenger of ROS, reversed the cordycepin-induced apoptosis effects. Theresults suggested that cordycepin may be a potential chemotherapeutic agent for the treatment of leukemia patients [This work was supported by Blue-Bio Industry RIC at Dong-Eui University as a RIC (08-06-07) program of ITEP under Ministry of Knowledge Economy].

The objective of this study is to develop 3D anthropometrical data acquisition method of human back surface and to recognise and extract its morphological characteristics. This technique is very useful in describing the sitter's back contour and the interaction between the sitter's back and the seat. The back surfaces of subjects in sitting posture are measured by using martine-type anthropometer and sliding gauge. These basic anthropometrical data are reconstructed into 3D image.