Sulfur is commonly used in Asia as a n herba l medicine to treat infl ammation and cancel‘. and potent chemopreventive effects have been demonstrated in various in vivo and in vitromodels for sulfur-containing compounds found in naturally occun‘ ing products. Here, we report the growth inhibitory and apoptosis-related effects of a newly developedhigh- purity eclible sulfur (ES) on immortali zecl human oral keratinocytes (IHOKs) and on oral cancer cells representing two stages of oral can cer (HN4‘ HN12) basecl on an 3-(4. 5-Dimethylt hiazol-2-yl)-2.5-cliphenyl tetrazolium bromide (MTT) a ssay, Western blotting, cell cycle analysis, ancl nuclear staining. The puri ty of the ES used in th is s tucly was verified by high performance liquid chromat ography (HPLC) , amino acid analysis and energy di spersive spectroscopy (EDS). ES inhibitecl the proliferation of immor talized and malignant oral kerati nocytes in a dose- and time-dependent manner FITC-Annexin V staining. DNA fragmentation testing. and Hoechst 33258 staining revealed that ES inhibits cell growth via apoptosis . ES blocked cell-cycle progression at the sub- Gl phase, with decreased expression 0 1' cyclins Dl, D2, and E, and t heir activating partners cdk2, cdk4, and cdk6‘ and a concomitant induction of p53 and p21/WAF1. Furthermore, ES treatment increased the cytosolic level of cytochrome c a nd resulted in caspase-3 activation‘ and thi s effect was correlated wi th Bax up- regulation and Bcl-2 down- regulation Taken together, these clata suggest that ES is a potential chemopreventive and chemotherapeutic agent for oral cancel

Heme oxygenase-l (HO-l) exhibits cyt oprotective effects in many different cell types and is induced by nicotine exposure in human gingival fibroblasts‘ However‘ therole of HO- l in cancer cells exposed to nicotine has not previously been descnbed We investigated the effects of nicotine on HO-l protein expression and cell viability in immortalized (IHOK) and malignant (HN12) human ora l keratinocyte cells using the MTT assay and Western blotting. We al so examined the involvement of t he phosphoinosit ide-3-0H- kinase (PI3K), mitogen-acti vated protein kinase (MAPK) , and nucJear factor-κ B (NF-κ B) signaling pathways in nicotine-induced cytotoxicity and HO- l levels in IHOK and HN12 cell s‘ Nicotine induced HO- l pro ducti on and had cytotoxic effects on cells in both a concentration- and time-dependent manner. Nicotine-induced cytotox icity and accumulation of HO- l were greater in JJ-IOK cells than in HN12 cells Molecular inhibitors of the ERK, p38 MAP kinase, PI3K, and NF-κ B signaling pathways blocked the cytotoxic effects and induction of J-IO-l expression by nicotine. Treatmen t with an t ioxida nts (bil irubin, N-acetyl cysteine) protected cells against nicotine-induced cytotoxicity and blocked the upregula tion of J-IO- l, the effects of which were more pronounced in II-IOK cells than in HN12 cells Collecti vely, these results suggest that J-IO- l plays a principal role in the protective response to nicotine in oral cancel and immortalized keratinocytes. Moreover, the addition of exogenous antioxidants may help to protect oral epithelial cells as chemopreventive agents against nicotine-induced oxidative stress.

The aim of t his study was to investigate the cytotoxic and ni t ric oxide (NO)-inducing effects of bismuth oxide (Bi203)-containi ng Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-l (HO-l) is involved in BPC-induced cytotox.icity in dental pulp cells Cytotoxicity and NO production induced by BPC were higher than those induced by Portland cement (PC) at 12 and 24 hours, and the former grad ua lly decreased to the level observed for PC. HO- l and inducible nitric oxide synthase (iNOS) mRNA expressions in the BPC group showed maximal increase at 24 hours. and it gradually decreased with increasing cultivation tlme Hemin treatment reversed the BPC-induced cytotoxicity ‘ whereas zinc protoporphyrin IX treatment increased the cytotoxicity. These results suggested that NO production by BPC correlates with HO-l exp1'ession in dental pulp cells Moreover ‘ BPC- induced HO-l expression in dental pulp cells plays a protective 1'ole against the cytotox.ic effects of BPC.

Previous in vi tro studies demonstrated that H202 or carbamide peroxide cou ld penetrate i nto pul p chambers through enamel and dentin (Benetti et a l., 2004; G okay et a l. , 2004‘ Suli eman et al .. 2005) ‘ Recently. Lee et al.(2006) demonstrated that H20Z enhanced the diffe rentiation of odontoblast like cell line, whereas it inhibited osteogenic diffe rentiation in pre 。steobl astic cell line, as seen by its efl"ecLs on an early difï"erentiation marker. ALP activity. I-lowever. the effects of HZ02 have not been well elucidated in primary cultured human pulp cells ln th is study‘ we investigated whether HO- 1 is involved in H20 2-induced cytotoxicity and examined the production 0 1" dent in sia lophosphoprotein (DSPP) and other minera li zation markers, in human pulp cells H20Z dec1'eased cell viabili ty. but increased HO-l and DSPP expression in a concentra t ion and time dependent manner. Inhibitors of guanylate cyclase, PI3K. ERK, and p38 MAP kinase blocked J-!?,0 2- induced cytot oxicity and the expression of HO-1 and DSPP mRNAs in pulp cells. These data suggest that t he induction of HO-l by H202 in pu lp cells plays a protective role against the cytotoxic effects of H202 and stimulates DSPP expression. resulting in prematu re oclontoblast differentiation th rough pathways t hat involve cGMP. p38. ancl ERK

Although substance P (SP). a potent pro-inflammatory peptide, is involved in inflammation and immune responses, the effect of SP 011 the expression of macl'ophage inJlammatol'Y protein 3a (MIP-3a. CCL20) in periodontal ligament (PDL) cells a l'e unknown Equally as enigmatic is the link between SP. the stress protein heme oxygenase-l (HO-l) , and CCL20 product ion. We investigated whether SP induces the release of chemokine CCL20 from irrunortalized POL (IPDL) cells. and further claif’y SP mediated pathways . We also exarnined the relationship between HO-l and CCL20 by treating POL cells with SP Incubating IPOL cells with SP incl'eased ex pl'ession of CCL20 mRNA and CCL20 protein in a dose-time dependent manner. Highly selective p38 and ERKl/2 inhibitors abl'ogated SP-induced expression of CCL20 lD IPOL cells SP is also responsible fo l' ini tiating phosphorylation of I/( B‘ degl'adation of IK B. and activation of NF-/( B. SP induced expression of HO-l in both a concentration- and time-dependent manner. and CCL20 refl ected similal' patterns. The inductive effects of SP on HO-l and CCL20 were enhanced by HO- l inducer hemin and the membrane-permea ble cGMP analog 8-bromo-cGMP Conversely, this pathway was inhi bited by the HO-l inhibitor zinc Pl'otoporphyrin IX (ZnPP IX) and the selective inhibitor of guanylate cyclase‘ 1H- [1. 2. 4]uxad iazole[4, 3-alquinoxal i n- 1-one (ODQ) We report hel'ein the pathway that connects SP a long with other modulators 0 1' neuroimmunoregulationto the induction of HO-1 and the inflanunatol'y mediatol' MIP- 3a /CCL20 in IPDL cel ls. which play an impol'tant role in the development 0 1' pe- I'iodontitis or inflammation during ol'thodontic tooth movement

Recovery 01' original form ancl function from c1amaged organs 01' tissues is olltmost goal of regenera tive meclicine. Va riolls methods such as moleclll ar biology. drug c1elivery system, biomaterials. tissue engineering have been s tllcliecl and appl iecl in that field . 1'he core factor of all 01' these kinds of efforts might be the cells including stem cells 01' progenitor cell s . AclllJt progenitor 01' stem cells have many advantages for therapeutic meclicine, inclucling free form ethi cal probl em. easiness in collection and clllture. Bone marrow‘ fat tissue, peripheral blood. placenta‘ ancl umbilicaJ cord bloocl a re preferable source 01' acllllt stem cells 01' progenitors. ]-]uman umbilical cord bloocl. taken form vein of corcl after baby c1elivery‘ are known to contain many progenitor cells. Since Boyse et al reportecl bone marrow transplantation with hllman cord blood CD34+ cells f'or leukemia. functional cells in human cord blood have been the cells of great interest. 1n this study‘ the a u thors i s이 ated peripheral bloocl mononuciear celJs. endothelial progenitors‘ late outgrowth vascular endothelial-like cells‘ ancl mesenchymal stem cell- like cells from human umbilical cord blood and a pplied in bony defects, myocardiac infarction and limb ischemic lesl0n Al I of these fllnctional cells showed favorable healing capacity and their effects primarily based on enhanced anglOgenesls Conclllsively. a lthollgb the precise cha racteristics are not well-described‘ the current stucly reveals that various types of functional cell s of human umbilical cord blood have some stem cell or progenitors features and play an important r ole in ti ssue regeneration

3BK21 program for Veterinary Science, College of Veterinary Medicine‘ Seoul National University. Seoul. Korea Human Co1'd blood has been used for the alternatives of bone marrow transplantation for more 10 years. Recently Mesenchymal s tem cell s , ES-like cells and endothelial stem cells has been successfuly isolated from huam co1'd blood Presentl y. it has been reported that a bout 70 incurable ans tractible di sease was possibly cured by umbili cal cord blood-deri ved s tem cells in the clinit;al test s‘ However‘ isolation and expansion of s tem cells from human umbilical cord blood(UCB) have been very difficult and an obstrucle for the clinical use This study showed that effi cient s iolat iona and expans ion of mesenchymal stem cells from UCB Full term UCB samples were obtained from the umbi lical vein after vaginal deli ve ry with the informed consent 0 1' the mothe1' approved by Borame Hospital Institutional Review Broad (IRB). And a lso. t his work was also a pproved by Seoul National University IRB. Recently, we isolated a population of s tem cells from human corcl bloocl (UCB)‘ which expressed embryo stage specific maker. SSEA-4. ancl the multi-potential stem cell marker‘ 。c t4 And we have sucessfully developed culture methods to expand ancl subculture these cells up to 1.000 billion from one single clone. Subsequently. we were a ble to transclifferente theses stem cells into insulin- producing is let- like structures. which co-express in sulin andC-pepticle, adipocyte, neuron‘ bone and cartilage. In acldition. the isola tion rate of MSC from UCB is about 70 % from the cord blood units. This isolation rate were not affected by maternal ages. the sex of baby, isolation time from the deli very. for example. 12 hrs. 24 hrs ‘ even 48 hrs from delivery Taken together. these findings might have a s ignificant potential to aclvance human UCB clerivecl stem- cell -basecl ther apeutics fOI' clinical use in near future

Human gingival fibroblasts are necessary for oral homeostaslS These cells are fundamental in tissue healing and tissuc remodeling processes under a response to physiological actions such as mastication, Collagen and elastin, that are extracell ular glycoprotein of gingival fibroblast, are found in all animals, '1γpe 1 collagen is most dominant protein found in human gingival fibroblasts , Matrix metalloproteinase-1(MMP-1) has a role play in destruction of metabolism of extracellular matrix(ECM) and MMP-1 can destroy many ECMs as well as non-ECM molecules MMP-1’s local activation is conytolled by tissue inhibitor of metalloproteinase-1(TIMP-1) , Therefore, it is important to have a balance between in both s ituations MMPs and TIMPs of increased 0 1' decreased extracelluar matrix molecules, The purpose of trus study is to find out the effect of physical stimulus to human gingival fibroblast on mRNA, proteins of collagen 1, elastin, MMP-1, and TIMP-1 Healthy human gingival fibroblasts were separated and cultur때 in DMEM(Dulbeco’s Modified Eagle’s Medium) , When the sample reached to confluence state, it was separated with 0,25% t rypsin and 0‘ 53mM ethylendiaminetetraacetic aCld Separated cells were centrifuged in a cell culturing flask at 1000rpm for 30, 60, 120 mmutes Then it was forced by 35g/cm' continuously, The obtained results that expression of mRNA using histological study and Reverse Transcription Polymerase Chain Reaction (RT-PCR) , expression of protein using Enzyme-Linked Immunosorbent Assay(ELISA) for this study, At 30minutes after cen trifuging, there were s pindl e shaped gingival fibroblasts with long processes parallel to other cells in the control group , However the cell density was simil ar to compared group, At 60minutes after centrifuging, spindle shaped human gingival fibroblast with relatively long process, less densely packed, At 120minutes after centrifuging, cell processes were lengthened 2-3 times‘ and cell density was lower, At 30-60 minutes after centrifuging, it was increased by 1,3-1,7 times in expressoin of collagen 1 mRNA as compared with comparison group, However, there was no change in elastin, TIMP-l, and MMP-1, At 120 minutes after centrifuging, The revealed collagen 1 mRNA was increased 3 times as compared with comparison group, It was increased 2 times in elastin , 12 times in TIMP-1 as compared with comparison group, However, there was no change in MMP-l. At 30-60 minutes after centrifuging, it was increased by 1.1 times in revealing of protein revealing in collagen 1, TIMP-1 But there was no cha nge in elastin, MMP-1 At 120 minutes after centrifuging, it was increased by 1,2 times in revealing collagen 1 protein, 11 times in elastin, 12 times in TIMP-l, but there was no change in MMP-l. ln conclu s ion, it increased in revelation of collagen 1 ,elastin and TIMP-1 by continous stimulus in human gi ngival fibroblast, But there was no change in revelation of MMP-l Therefore, th is type of pressu re is one of the components for healing of gingiva l fibroblast

Laser is used to prevent the early dental caries in dental f ield and to apply for treatment of stomatitis and hyper sens it ivity , and laser mass Recently it is reported that laser i1'r adiation affect on soft tissue treatment and bone 1'emodelling after dental implantation. The purpose of this study was to examine laser irradia ti on effect on activity of normal human osteoblast on titanium plate in vitro by various laser wave length, and to observe morphologic change of NHost on LiLa nium plate and to a nalysis concentration of Ca"++ , IP and ALP NHost were cultured in DMEM containing 10% FBS, and observed by in verted microscope 1"or attatchment to the surface of titanium plate. Ca ++, I.P. , and a lkaline phosphat ase(ALP) concent ration in medium was calculated during 4 weeks, which was treated with Wilcoxon rank, Anova test and linear regression. The obtained results were as follows Morphologic changes showed rapid growth rate of NHost ++ at 3 days of laser lrradiatlOn ln spite of laser wave type, Ca" and P concentration was decreased at 2 weeks and was the hig hest at 3 weeks, but decreased at 4 weeks In spite of laser wave type, ALP concentration was decreased at 2 weeks but was increased at 3-4 weeks, From the aboving results, in spite of laser wave type, there were rapid growth rat e of NHost a nd no significant of Ca"++ , IP and P concen tr ation but these concentration showed predominant change than that of control

Many researchers are interested in wound healing in the t reatment of burns, prevention of post surgical adhesions and cosmetic s urgery by excess collagen production and scar formatlOn Synthetic epidermal substi tutes with cultured epi thelial cells seem to be an attractive strategy since keratinocytes have been demonstrated to modulate fibroblast growth and collagen synthesis. Bioa bsorbable and biocompatible chitosan structurally mimics hyaluronic acid. Recently, a bio compatible synthesi zecl ch itosa n-PVP(polyvinyl pyrrolidone) hydrogels demonstrated in vitro biocompat ibi li ty for bio medical applications . However. there is no re port on this hydrogeJ"s ability to modulate human gingival fibroblast growth. The purpose of this study were to investigate different growth modulation between human gingival fibroblast and normal human oral keratinocyte by chitosan- PVP hydrogel, and to apply this biocompatible synthetic polymer to oral and maxillofacial wound healing. We have synthesized a hydrogel from chitosan-PVP and examined its effect on human gingival fibroblast growth modulation in vitro. Non-toxic and biocompatible hydrogel with human gingival fi broblasts and epithelial cells was tested by MTT assay. HGF showed a higher growth proliferation than that of NHOK after cell seeding. In MTT assay, 30% hydrogel leach out products showed a higher cellular viability in NHOK than that of any other products. In MTT assay, 30% hyclrogel leach out products showed relatively lower cellular viability of HGF ln growth profile, NHOK showed about 7 fo lcls higher than HGF after 1 day, while about 2 fo lds higher after 5 days. And also NHOK showed above about 70% cell ular via bility from 1 to 7 days. It suggested that Chitosan-PVP hydrogel would inhibit relatively the growth of HGF and s timulate the growth of NHOK_ This phenomenon may prove to be of use in wound management 0 1' oral and maxillofacial area as epitheli al substitutes.

본 논문의 목적은 인간작업모델(The Model of Human Occupation: MOHO)을 이론적 틀로 하여 개발된 근로 자역할인식 면담도구(Worker Role Interview: WRI)를 소개하고, 향후 국내에서의 적극적인 사용을 제안하기 위한 것이다. WRI를 이용한 면담조사를 준비하기 위하여 2006년 9월부터 12월까지 WRI Version 9.0의 매뉴얼을 한국어로 1차 번역을 실시하였고, 감수와 수정 과정을 통하여 면담조사 권장질문지와 점수표, 판정기준을 마련하였다. 면담조사를 위하여 작업치료실이 있는 3곳의 산재전문병원을 방문하여 연구의 취지를 설명하였고, 그 중 한 곳에서 직업복귀 가능성이 있고 연구의 취지에 동의하는 산재 환자를 소개받아 2007년 2월 27일 면담조사를 실시하였다. WRI의 평가결과는 최소 17점에서 최대 68점으로 점수화 할 수 있는 데, 클라이언트의 평가 결과 총점 48점으로 전체적으로 직업복귀에 부정적인 측면 보다는 긍정적인 측면이 더 큰 것으로 나타났다. 특히 개인적 사유나 자신의 직업에 대한 가치, 흥미와 관련되어 있는 의지 영역에서 직업복귀를 강하게 지지하는 반응을 보였는데, 이는 클라이언트의 직업복귀에 대한 동기가 강하므로 직업복귀에 성공할 가능성이 높다는 전제 하에 재활서비스가 제공되어야 함을 의미한다. 인간작업모델이라는 새로운 작업치료의 패러다임을 임상에서 적용할 수 있는 간편한 면담평가도구로써 WRI는 심리적, 사회적, 환경적 변인을 통한 직업복귀의 가능성을 예측한다는 점에서 의미가 있으며, 작업치료사가 환자의 기능적 손상이나 장해에만 관심을 두는 것이 아니라 보다 폭넓은 관점에서 환자를 이해하고 접근하여 성공적인 재활프로그램을 계획하는데 도움이 되리라 기대된 다.