Saturnia (Rinaca) jonasii Butler, 1877 is distributed in Japan, including Tsushima Island and Taiwan, whereas S. boisduvalii Eversmann, 1846 is distributed in northern areas, such as China, Russia, and South Korea. In the present study we found that the specimens from Mt. Hallasan on Jejudo, a southern remote offshore island, were S. jonasii, rather than S. boisduvalii based on morphology, DNA barcode, and nuclear elongation factor 1 alpha (EF-1α) sequences. The major morphological differences between the two species included the shape of wing pattern elements of fore- and hindwings and male and female genitalia. A DNA barcode analysis of the sequences of the Jejudo specimens and S. boisduvalii, along with those of Saturnia species obtained from a public database showed a minimum sequence divergence of 4.26% (28 bp). A phylogenetic analysis also showed clustering of the Jejudo specimens with S. jonasii, separating S. boisduvalii (Bayesian posterior probability = 0.99). The EF-1α-based sequence and phylogenetic analyses of the two species from Jejudo Island and the Korean mainland showed the uniqueness of the Jejudo specimens from S. boisduvalii collected on the Korean mainland, indicating distribution of S. jonasii on Jejudo Island in South Korea, instead of S. boisduvalii.

The spotted-wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), originally distributed across a few Asian countries, including South Korea, has invaded North America and Europe, but is absent from Australia. In order to export the South Korean grape cultivar Campbell Early to Australia, its potential to serve as oviposition and development medium for SWD must first be determined. In this study, we determined the oviposition and development potential of SWD on Campbell Early, after elucidating the SWD life cycle and establishing an artificial diet-based mass-culturing system. An investigation of the life cycle under five temperature regimes (16, 19, 22, 25, and 28°C) showed that the durations of the egg, larval, and adult stages were shorten when temperature was increased from 16, 19, 22, 25, and 28°C, but pupal duration was shortest at 25°C and extended again at 28°C. A test of oviposition and development potential of SWD on Campbell Early grape clusters showed oviposition of 30.8 ± 6.8 eggs per cluster of injured grapes and 157.7 ± 16.2 eggs on a culture dish of artificial diet. However, in a similar experiment using uninjured grape clusters, only a single egg was deposited on the grape skin, which soon dried. In light of these results, newly harvested grapes left at vineyards during daily harvests are unlikely to serve as an oviposition and development medium for SWD, as long as the grapes remain uninjured.

Kentrochrysalis streckeri Staudinger, 1880 is distributed in Russia, Mongolia, China, and North Korea, whereas K. consimilis Rothschild & Jordan, 1903 has been known to occur South Korea and Japan. In the present study we found that the specimens from South Korea were K. streckeri, rather than K. consimilis based on morphology, DNA barcode, and nuclear elongation factor 1 alpha (EF-1α) sequences. The major morphological differences between K. streckeri and K. consimilis include the shape of wing pattern elements of fore- and hindwings and male and female genitalia. A DNA barcode analysis of the South Korean specimens and K. streckeri originated from Russia showed a maximum sequence divergence of only 0.659% (four bp), whereas the barcoding sequence of K. consimilis sequenced in this study and GenBank-registered sequence (JN678086), both of which originated from Japan showed the minimum sequence divergence of 2.965% (18 bp), indicating that the Korean specimens are, in fact, K. streckeri, instead of wrongly known K. consimilis. Phylogenetic analyses both by Bayesian Inference and maximum likelihood methods well supported the monophyly of South Korean specimens and Russian K. streckeri, excluding K. consimilis. The EF-1α-based sequence and phylogenetic analyses of the two species also supported the data from DNA barcode, indicating distribution of K. streckeri in South Korea, instead of K. consimilis.

The lycanid butterfly, Shijimiaeoides divina (Lepidoptera: Lycaenidae) is listed as the second-degree endangered wild animal in Korea from 2012. The 15,259-bp long complete mitochondrial genome of the species consisted of a typical set of genes (13 protein-coding genes, two rRNA genes and 22 tRNA genes) and one major non-coding A+T-rich region, with the typical arrangement found in majority of Lepidoptera. The 15,259-bp long S. divina mitogenome is well within the range found in Lycaenidae and has typical sets of 37 genes and a major non-coding A+T-rich region as 379 bp. As other lycanid butterflies S. divina COI also started with CGA. The gene arrangement of S. divina is identical to that of the Ditrysia in Lepidoptera that has the order trnM-trnI-trnQ (underline for inverted gene) between the A+T-rich region and ND2. Comparison of the skewness between the PCGs encoded in major and minor strand indicates a substantial difference between them in GC skewness (0.261 ~ 0.340 in minor strand vs. -0.081 ~ -0.115 in major strand). The 151-bp intergenic spacer sequence of the S. divina mitogenome is spread over 16 regions ranging in size from 1-53 bp. The longest one (53 bp) located between trnQ and ND2 shows substantially high sequence homology to neighboring ND2 may indicating the origination of the region by a partial duplication of the ND2 gene. One of the unusual features of the S. divina mitogenome is the presence of a trnK-like sequence that is encoded at the major strand of the genome in the A+T-rich region.

The genus Toxares (Braconidae: Aphidiinae) is reported for the first time in Korea. Members of Toxares are known as solitary koinobiont endoparasitoids especially specific for aphidine aphids. In this study, Toxares deltiger (Haliday, 1833) is found as new to Korea, which has a wide host range for 31 aphid species within Aphidinae. Thus, this species would be useful as natural enemy for biological control in the future. Diagnosis and host information are included.

Mycoplasma hyorhinis (M. hyorhinis) is considered an etiological agent of arthritis in suckling pigs. Recently, some M. hyorhinis strains were shown to produce pneumonia that is indistinguishable from the mycoplasmosis caused by M. hyopneumoniae. In this study, we developed a sensitive and specific PCR assay to detect M. hyorhinis and applied the developed PCR assay for detection of Mycoplasma infection in clinical piglets infected with M. hyorhinis. We developed a new PCR assay using a M. hyorhinis-specific primer pair, Mrhin-F and Mrhin-R, designed from the Mycoplasma 16S-23S rRNA internal transcribed spacer (ITS) region. The primers and probe for the assay were designed from regions in the Mycoplasma 16S-23S rRNA ITS unique to M. hyorhinis. The developed PCR assay was very specific and sensitive for the detection of M. hyorhinis. The assay could detect the equivalent of 1 pg of target template DNA, which indicates that the assay was very sensitive. In addition, M. hyorhinis PCR assay detected only M. hyorhinis and not any other Mycoplasma or bacterial spp. of other genera. The new developed PCR assay effectively detected M. hyorhinis infection in pigs. We suggest that this PCR assay using a M. hyorhinis-specific primer pair, Mrhin-F and Mrhin-R, could be useful and effective for monitoring M. hyorhinis infection in pigs.

In recent decades, there has been an increasing interest in the use of carbon fiber reinforced plastic (CFRP) in aerospace, renewable energy and other industries, due to its low weight and relatively good mechanical properties compared with traditional metals. However, due to the high cost of petroleum-based precursors and their associated processing costs, CF remains a specialty product and as such has been limited to use in high-end aerospace, sporting goods, automotive, and specialist industrial applications. The high cost of CF is a problem in various applications and the use of CFRP has been impeded by the high cost of CF in various applications. This paper presents an overview of research related to the fabrication of low cost CF using polyethylene (PE) control technology, and identifies areas requiring additional research and development. It critically reviews the results of cross-linked PE control technology studies, and the development of promising control technologies, including acid, peroxide, radiation and silane cross-linking methods.

The advancement of industry and science in modern society has facilitated the inherent growth of humanity due to the provisions of large amounts of information and knowledge. Deconstructionism originated from the desire for self-expression rooted in the inherent growth of humanity and desires to express various characteristics through deconstruction and not be classified and constrained by existing dichotomous structures and forms. Deconstructism (an idea to resist dichotomous ideas and existing structuralism) seeks androgyny as well as the deconstruction of sex vis-à-vis human desire. Human androgyny, inherent in history and ancient Greek myths (Kwon, 2002), is now being adapted by modern society, psychology, culture, and art (Han & Kim, 2012). Androgyny, adapted by literature, sculpture, and painting, is now being used by the production of various images as well as modern fashion costumes. It is necessary to study androgyny to provide an aesthetic value for modern fashion design that can also deeply analyze the ideas and philosophies of fashion designers. This study considers androgyny with a focus on deconstructive fashion, understanding the tendency of androgyny in modern fashion, and providing a reference material for future fashion designs as well as for the direction and development of various fashion accessories and hair accessories. This study uses internet resources and previous research from professional journals and books about deconstructionism and androgyny for a literature review. It adapts an empirical method based on visual images from the design collections of designers defined as deconstructive fashion designers by researchers and professional editors. The androgyny of deconstructive fashion is classified in this study as follows. The first are ambiguous designs that show no clear distinction between sexes. The designers show designs unconstrained by sexes and which seek human instinct through wearing fashion items of the opposite sex that use masks and express sexual ambiguity with makeup and costumes. The second is integration shown in designs with both masculine and feminine characteristics. The designers integrates the lines and details of the opposite sex with costumes and patterns to escape existing ideas and show a silhouette that eliminate sexual stereotypes. The third is deconstructivity as shown in designs with no sexual characteristic and no sexual concept. It is a trend that originated from the interpretative view that adapts the super realism of deconstructionism and is free from an existing aesthetic consciousness to deconstruct a sex role for existing costumes and refuse all standardized ideas; consequently, the roles of costumes and items are eliminated and a new fashion style emerges from the individual values and ideas of designers. The androgyny of modern fashion from deconstructionism diverges from traditional form, integrates with aesthetic factors, and becomes one of a modern style that also provides more creative design motifs for fashion designers.

Curcumin (diferuloylmethane), a constituent of turmeric powder derived from the rhizome of Curcuma longa, has been shown to inhibit the growth of various types of cancer cells by regulating cell proliferation and apoptosis. However, a need exists to design more effective analogs because of curcumin's poor intestinal absorption. EF-24 (diphenyl difluoroketone), the monoketone analog of curcumin, has shown good efficacy in anticancer screens. However, the effects of curcumin and EF-24 on salivary gland epidermoid carcinoma cells are not clearly established. The main goal of this study was to investigate the effects of curcumin and EF-24 on cell growth and induction of apoptosis in human salivary gland epidermoid carcinoma cells. Our studies showed that curcumin and EF-24 inhibited the growth of HTB-41 cells in a dose- and time-dependent manner, and the potency of EF-24 was > 34-fold that of curcumin. Treatment with curcumin or EF-24 resulted in nuclear condensation and fragmentation in HTB-41 cells, whereas the control HTB-41 cell nuclei retained their normal regular and oval shape. Curcumin and EF-24 promoted proteolytic cleavages of procaspase-3/-7/-9, resulting in an increase in the amount of cleaved caspase-3/-7/-9 in the HTB-41 cells. Caspase-3 and -7 activities were detected in viable HTB-41 cells treated with curcumin or EF-24. These results suggest that the curcumin and EF-24 inhibit cell proliferation and induce apoptosis in HTB-41 human salivary gland epidermoid carcinoma cells, and that they may have potential properties as an anti-cancer drug therapy.

Genetic polymorphisms within immunity-related candidate genes in pigs have been identified to control variations in immune functions and/or disease resistance. It has become necessary to evaluate the effects of other genetic markers of economically important traits prior to introducing them into marker-assisted selection programs. In this study, polymorphisms of porcine genes coding Interferon-induced Gunylate binding protein 1 (GBP1), GBP2, CD163, and CD169 were investigated for their association with growth and meat quality traits in a Korean native pig breed -Yorkshire inter-crossed F2 pig population (KY-F2). KY-F2 animals (n=346) have been successfully used for linkage mapping to identify quantitative loci that control meat quality, growth, and immunity traits. In our results, polymorphisms in genes GBP1 and GBP2 showed association with pig growth rate as well as meat quality traits such as crude fat, drip loss, and meat color (yellowness) in the KY-F2 population. The polymorphism in gene CD163 only showed association with crude fat, as a meat quality trait. CD169 gene was associated with pork tenderness. In conclusion, four immune-related genetic markers were validated for their association with growth and meat quality traits to gauge their potential use in a swine selection program. The results warrant further studies in other commercial pig populations.

Osteoclasts originated from hematopoietic stem cells are multi-nucleated cells that can resorb the bone matrix. Receptor activator of nuclear factor kappa-B (RANK)/RANK ligand (RANKL) signaling pathway is crucial for the differentiation and activation of osteoclasts. In this study, we investigated for the first time whether or not RANKL induced mitogen- and stress-activated kinase 1 (MSK1) phosphorylation at Ser 376. Activation of MSK1 was detected as soon as 5 min after RANKL stimulation and sparsely detected at 30 min after stimulation. RANKL-induced MSK1 phosphorylation occurred in a dose-dependent manner. MSK1 is known as a downstream signaling molecule of cAMP-dependent protein kinase (PKA). Treatment with the PKA inhibitor H89 significantly suppressed c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) induction upon RANKL stimulation. In addition, cAMP response element-binding protein (CREB) phosphorylation was extremely inhibited by H89 treatment. Mitogen-activated protein kinases (MAPKs) have been investigated for induction of MSK1 phosphorylation. Specific signaling pathway inhibitors for p38 and extracellular signal-regulated kinases (ERKs) significantly blocked RANKL-induced MSK1 activation. Finally, as a downstream effector of the p38-MSK1 pathway, c-Fos transcriptional activity was determined. RANKL-mediated elevation of c-Fos transcriptional activity was significantly suppressed by p38 inhibitor. Moreover, a dominant negative form of CREB suppressed activation of NFATc1. In conclusion, RANKL-stimulated MSK1 phosphorylation could play a role in induction of NFATc1 through CREB and c-Fos activation as a downstream molecule of p38, ERK MAPKs, and PKA. Our results support basic information for the development of osteoclast specific inhibitors.

The Maillard reaction is a complex reaction that occurs between carbonyl and amine groups during food pro-cessing and storage. In addition, it produces a large number of Maillard reaction products (MRPs), which have an importantrole in determining food characteristics including aroma, color, flavor, and texture. Importantly, recent studies have beenconducted that the Maillard reaction products and their ferments with lactic acid bacteria (LAB) induced specific biologicalcharacteristics including antimicrobial, antioxidative, and antihypertensive activities as well as improved their physiologicalfeatures such as the heat stability and emulsifying properties. Therefore, we described on new insights for enhanced physi-ologic and biologic functions of MRPs through LAB fermentation.

Proper oral hygiene is required to maintain oral health and prevent oral disease. Toothbrushing is central to proper oral hygiene. Mechanical tooth cleaning is the mainstay of plaque control. A variety of toothbrushing techniques have been developed and evaluated for their efficacy. However, these evaluations are subjective. To adequately evaluate toothbrushing objectively, a novel toothbrushing monitoring system was developed. The system involves user-monitored brushing patterns including toothbrush motions using a 3-axis gyroscope, 3-axis accelerometer, 3-axis magnetic sensor, one load-cell and Bluetooth devices. To confirm the efficacy of this toothbrushing monitoring system, eight periodontist performed tooth brushing in a dental model, and their brushing motion was monitored and recorded, and evaluated by statistical means. The proposed monitoring system can be used to aid dental care personnel in toothbrushing instruction.

In this paper, the forward and inverse kinematics equations for demolition fire vehicle with 6-DOF were developed. This was done through the development of an interface that allows co-simulation using MATHEMATICA and ADAMS. The implementation of this project was done entirely in MATHEMATICA by calculating the kinematics equations to demonstrate the usefulness of this product. The forward and inverse kinematics of the demolition fire vehicle represents the position and orientation of the end-effector as any desired location. This research was particularly conducted using the simulator, and it showed good approximation results in terms of solving a reaching task problem.

We quantified temporal and spatial changes in the habitat for fish populations, the distribution of mandarin fish(Siniperca scherzeri) and an introduced species, largemouth bass(Micropterus salmoides) in Lake Paro and inflowing streams. The number of fish species identified in Lake Paro and the tributary streams included 10 families, 24 species and 10 families 30 species, respectively. The dominant fish species in Lake Paro were Zacco platypus, Hemibarbus labeo, Squalidus gracilis majimae, S. scherzeri and Tridentiger brevispinis, Z. platypus, Z. koreanus, and S. gracilis majimae in the inflowing streams. Although the habitat segregation for S. scherzeri and M. salmoides occurs, these two species showed the use of the fishes of the family Gobiidae as an important prey item based on IRI analysis. S. scherzeri and M. salmoides preyed mainly on T. brevispinis(67.4 %) and R. brunneus(84.0 %), respectively. The species preyed on by S. scherzeri and M. salmoides were benthic fishes that inhabit shallow water depths around the lake and have little swimming ability.

This study was conducted to investigate the distributional characteristics of ground beetles and to provide basis information for biodiversity management including the ground beetles in the Naejangsan National Park area. Pitfall traps were installed throughout 20 sites within Naejangsan National Park during 2008 to 2011 to collect ground beetles. A total of 2,409 collected ground beetles were identified with 35 species belonging to 19 genera of 8 subfamilies. Coptolabrus jankowskii jankowskii, Eucarabus sternbergi sternbergi, and Pterosticus audax were dominant at the core area, while Pheropsophus jessoensis, Synuchus nitidus, Synuchus cycloderus, and Chlaenius naeviger were dominant at the border of the National Park and adjacent to the road or grassland. These differences of dominant species also affected to the similarity of species composition between core and border area, and caused increasing dissimilarities between sites with cluster analysis. Although the result of the present study was a case study using ground beetles, it will be helpful to develop a management strategy of biodiversity conservation in Naejangsan National Park and its surroundings.

본 연구는 만다린 감귤에 대한 썩덩나무노린재 흡즙이 낙과 및 저장 중 부패에 미치는 영향을 검토하기 위하여 실시되었다. 과실 착색 전 흡즙 은 심한 낙과를 유발하였으나 착색 후 흡즙은 낙과율이 낮았다. 하지만, 부패유발 측면에서는 수확과실 및 착색전후 과실에 대한 썩덩나무노린재 흡즙이 저장 중에 부패를 유발하지 않았다. 많은 감귤농가에서는 썩덩나무노린재로 인한 낙과와 부패를 방지하기 위하여 생육후기 살충제를 자주 살포하고 있기 때문에 본 결과는 생육후기 이 해충 관리에 유용한 정보를 제공할 것이다.