In this study, the layered structures of immiscible Fe and Cu metals were employed to investigate the interface evolution through solid-state mixing. The pure Fe and Cu powders were cold-consolidated by high-pressure torsion (HPT) to fabricate a layered Cu-Fe-Cu structure. The microstructural evolutions and flow of immiscible Fe and Cu metals were investigated following different iterations of HPT processing. The results indicate that the HPTprocessed sample following four iterations showed a sharp chemical boundary between the Fe and Cu layers. In addition, the Cu powders exhibited perfect consolidation through HPT processing. However, the Fe layer contained many microcracks. After 20 iterations of HPT, the shear strain generated by HPT produced interface instability, which caused the initial layered structure to disappear.

연중생산이 가능하고 특히, 저온에서 화색발현이 우수하며 볼륨감 있는 스프레이국화 신품종을 개발하기 위하여 충청남 도농업기술원 화훼연구소에서 2010년에 모본으로 황색의 홑꽃 ‘SP09-251-01’를 부본으로 ‘SP08-010-16’계통을 인공수분 하였다. 종자 파종은 2011년에 실시하였고, 실생개체 중 화색과 화형이 우수한 281번째 개체를 선발하여 ‘SP11-103-05’로 계통명을 부여하였다. 주년생산성 검정을 위하여 2011년부터 2013년까지 자연일장, 전조재배, 차광재배를 각각 실시하였고, ‘Yes Holic’과 화형, 화색, 초형이 비슷한 ‘Jazz’ 품종을 대조품 종으로 사용하였다. ‘Yes Holic’ 품종은 2013년에 국립종자원에 품종보호 출원을 하였다. ‘Yes Holic’은 황색(Yellow 7A)의 꽃잎과 녹색(Green 1B)의 화심색으로 조사되었고 자연일장 작형의 개화기는 10월 25일로 ‘Jazz’의 10월 26일에 비해 빨랐다. ‘Yes Holic’의 초장과 줄기굵기는 각각 80.2cm와 6.1mm 로 ‘Jazz’의 78.6cm와 5.3mm보다 컸다. ‘Yes Holic’의 꽃 직경은 4.4cm로 ‘Jazz’의 4.1cm보다 컸으며, 착화수도 ‘Yes Holic’ 가 29.4개로 ‘Jazz’의 22.1개보다 많았다. 흰녹병저항성은 ‘Yes Holic’이 10%이하의 발병율인 3단계로 흰녹병이 저항성이 높은 것으로 확인되었다. 재배상 유의사항은 ‘Yes Holic’은 소등 후 야간온도를 18℃로 가온하면 고유화색 발현과 적당한 착화수 확보 및 균일한 개화가 가능하다. 그리고 여름철 고온기에는 ‘Yea Holic’의 총포에 잿빛곰팡이병이 주로 발생되므로 스크린과 포그를 활용하여 주간온도를 낮추고 봉오리 착색기에 전용 농약을 살포해 주어야 한다.

This study was conducted to evaluate the effect of rumen origin lactate-utilizing bacteria (LUB) as one of the potential treatments on subacute ruminal acidosis (SARA) during in vitro challenge compared to buffering agents (NaHCO3, sea minerals, MgO) and direct-fed microorganism (yeast). We hypothesized that rumen LUB (RLUB) could be a potential treatment to treat ruminal acidosis. The supplementation level of other treatments was determined by referring to previous studies in the literature. The 108 CFU/g freeze-dried RLUB isolated from Hanwoo cattle were compared with 0.1% NaHCO3, 0.8% of MgO, 0.5% sea mineral and 0.4% yeast during in vitro SARA challenge. Rumen fluid collected from one cannulated Holstein and one Hanwoo steer fed by maize silage was mixed with 0.5g feed consisted of 0.05g forage and 0.45g concentrate. These mixtures were incubated in triplication for 3, 6, 12 and 24h. After 6h of incubation, along with MgO and sea minerals, RLUB treatment showed higher (p<0.05) ㏗ values than control with no significant differences in total volatile fatty acid concentration. However, in the same period, the propionate concentration and A:P ratio were higher in RLUB than in the other treatment (p<0.05), which might alter the fermentation pattern. On the other hand, the RLUB treatment produced a higher (p<0.05) ammonia-N concentration. Based on these results, we can conclude that RLUB might have the potential to alleviate SARA. Nonetheless, further study on its mechanism in SARA is required, especially with live animals.

A pregnancy diagnosis is an important standard for control of livestock’s reproduction in paricular dairy cattle. High reproductive performance in dairy animals is a essential condition to realize of high life-time production. Pregnancy diagnosis is crucial to shortening the calving interval by enabling the farmer to identify open animals so as to treat or re-breed them at the earliest opportunity. MicroRNAs are short RNA molecules which are critically involved in regulating gene expression during both health and disease. This study is sought to establish the feasible of circulating miRNAs as biomarkers of early pregnancy in cattle. We applied Illumina small-RNA sequencing to profile miRNAs in plasma samples collected from 12 non-pregnant cows (“open” cows: samples were collected before insemination (non-pregnant state) and after pregnancy check at the indicated time points) on weeks 0, 4, 8, 12 and 16. Using small RNA sequencing we identified a total of 115 miRNAs that were differentially expressed weeks 16 relative to non-pregnancy (“open” cows). Weeks 8, 12 and 16 of pregnancy commonly showed a distinct increase in circulating levels of miR-221 and miR-320a. Through genome-wide analyses we have successfully profiled plasma miRNA populations associated with pregnancy in cattle. Their application in the field of reproductive biology has opened up opportunities for research communities to look for pregnancy biomarker molecules in dairy cattle.

In this study, soybean oil, which is used in a large variety of processed foods, is used as a carbon source. Soybean oil is successfully coated onto the surface of LiNi1/ 3Co1/3Mn1/3O2 (NCM) by a simple method. The physical and electrochemical properties of NCM/C hybrid materials are determined. As a result, a 5 nm thickness carbon coating layer is formed on the surface of the NCM, resulting in improved capability and cyclic performance in the battery. The NCM/C battery shows an initial discharge capacity of 159 mAh g−1 and 95% capacity retention after 100 cycles (a discharge capacity of 120 mAh g−1 and 94% retention are observed after 100 cycles for the NCM cathode).

Iron and copper are practically immiscible in the equilibrium state, even though their atomic radii are similar. As non-equilibrium solid solutions, the metastable Fe-Cu alloys can be synthesized using special methods, such as rapid quenching, vapor deposition, sputtering, ion-beam mixing, and mechanical alloying. The complexity of these methods (multiple steps, low productivity, high cost, and non-eco-friendliness) is a hinderance for their industrial applications. Electrical explosion of wire (EEW) is a well-known and effective method for the synthesis of metallic and alloy nanoparticles, and fabrication using the EEW is a simple and economic process. Therefore, it can be potentially employed to circumvent this problem. In this work, we propose the synthesis of Fe-Cu nanoparticles using EEW in a suitable solution. The powder shape, size distribution, and alloying state are analyzed and discussed according to the conditions of the EEW.

We investigated the cytotoxic potential of three different commercially available absorbent feminine hygiene products and one transdermal patch using direct contact and extract exposure methods. Two different cell lines were used – mouse fibroblast L929 and normal human skin fibroblast CCD-986sk cell lines. The test samples were extracted using three different methods in accordance to International Organization for Standardization (ISO). Viability of cells was analyzed using MTT assay and morphology of the cells were also observed using phase contrast microscopy. Overall, the direct contact method using L929 cells showed that all the test samples had no toxic effect when exposed to extracts for 1 h. For the exposure method, no toxic effect was observed in both L929 and CCD986sk cells incubated with all the test samples regardless of the extraction methods used.

Recently there was an incident that military radars, coastal CCTVs and other surveillance equipment captured a small rubber boat smuggling a group of illegal immigrants into South Korea, but guards on duty failed to notice it until after they reached the shore and fled. After that, the detection of such vessels before it reach to the Korean shore has emerged as an important issue to be solved. In the fields of marine navigation, Automatic Identification System (AIS) is widely equipped in vessels, and the vessels incessantly transmits its position information. In this paper, we propose a method of automatically identifying abnormally behaving vessels with AIS using convolutional autoencoder (CAE). Vessel anomaly detection can be referred to as the process of detecting its trajectory that significantly deviated from the majority of the trajectories. In this method, the normal vessel trajectory is gridded as an image, and CAE are trained with images from historical normal vessel trajectories to reconstruct the input image. Features of normal trajectories are captured into weights in CAE. As a result, images of the trajectories of abnormal behaving vessels are poorly reconstructed and end up with large reconstruction errors. We show how correctly the model detects simulated abnormal trajectories shifted a few pixel from normal trajectories. Since the proposed model identifies abnormally behaving ships using actual AIS data, it is expected to contribute to the strengthening of security level when it is applied to various maritime surveillance systems.

Many parrots are considered endangered species due to threats from human activities. Gender determination is of great importance for biological studies and the conservation of endangered parrots. However, like other birds, gender determination in parrots is hindered due to the lack of external dimorphism between males and females. A molecular approach using the chromo-helicase-DNA binding protein 1 (CHD1) gene is commonly used for sexing birds. This study aimed to determine the gender of parrots from Korean zoos based on amplification and visualization of the partial CHD1 gene. The samples of 13 parrot species were collected from three different zoos in Korea and the extracted DNA templates were amplified using CHD1 gene primers. The gender of 27 samples of 13 species was determined by visualizing the PCR products on an agarose gel. While male parrots were indicated by a single band, female parrots were indicated by double bands. The findings provide additional information, which might be helpful for the management and care of parrots in Korean zoos.

These experiments were to investigate the variations of rye on forage quality, productivity and β-carotene concentration affected by maturity in Pyeongchang region. Limited information are available about how forage quality and β-carotene content are affected by various factors. Samples were collected from rye harvested every 5 days, from April 25 to May 31 (April 25, April 30, May 4, May 9, May 15, May 21, May 25 and May 31). Dry matter (DM) content, plant height, DM yield and total digestible nutrient (TDN) yield increased continuously with the progressed maturity. However, crude protein (CP) content, in vitro dry matter digestibility (IVDMD) and relative feed value (RFV) decreased markedly with the delay of harvesting, while TDN content decreased from April 25 till May 15, then followed by a stable fluctuation. Conversely, acid detergent fiber (ADF) and neutral detergent fiber (NDF) value increased and then fluctuated slightly after blooming stage. For quality of plant parts, stem contained the lowest CP content and RFV value, and the highest ADF and NDF contents compared with other parts, while the grain showed the higher CP, IVDMD, RFV and lower fiber contents than others. With the plant matured, leaf proportion decreased while stem and grain proportion increased, and feed value of all the three parts decreased till blooming stage and followed by a stable phase. β-carotene concentration showed its highest on jointing stage, and then fell down sharply on the sequential stages. In conclusion, harvest around May 15 (blooming) is proper for forage rye if directly consumed by livestock as green chop in Pyeongchang under the consideration of both nutritive yield and forage quality.