본 연구는 국내 차나무 품종을 대상으로 연평균 기온이 낮은 봉화군에 식재하여, 봉화지역 차나무 재배 가능성을 검토하고자 연구를 수행하였다. 봉화군 시험지 토양 조사 결과, 시험지별 토양의 pH, EC, 치환성양이온, 유효인산은 적정수준이었으며, 유기물 함량은 대체로 낮은 수치로 조사되었 다. 봉화군 시험지 기온 조사 결과, 최저 기온은 소천면이 -14.1℃로 가장 낮았고, 다음으로 춘양면 -12.6℃, 명호면 -12.6℃, -10.2℃ 순으로 모든 시험지는 -10℃ 이하의 낮은 온도로 조사되었다. 차나무 재배 생육평가 결과, 명호면 시험지에서 생육 정도가 가장 우수하였고, 다음으로 소천면, 온실, 춘양면 순으로 조사되었다. 또한, 재배품종 보향과 대조품종 다산은 시험지 생장량과 광합성량이 다른 수종에 비해 높은 것을 확인하였 다. 봉화지역과 하동지역에 식재된 참녹의 주요 생리활성물질인 catechin과 caffeine 함량을 조사한 결과, Catechin 함량은 EGC와 EC, EGCG 함량은 하동이 높게 조사되었고, 반면 ECG는 봉화지역의 참녹이 월등히 높았다. 총 catechin 함량은 봉화 5.23%, 하동 5.22%로 비슷하게 나타났다. Caffeine 함량은 봉화 0.05%, 하동 0.38%로 하동지역의 참녹이 높게 나타났다. 본 연구에서 봉화군 차나무 재배 생육평가 결과 명호면, 소천면이 차나무 재배에 유리할 것이며, 봉화지역 차나무 재배 시 재배품종 보향과 대조품종 다산은 재배 적응성이 높을 것으로 판단된다.

Since last year, the government has enforced the 'Act on the Punishment of Severe Accidents, Etc.' (hereafter referred to as the 'Serious Accident Punishment Act'), which punishes business owners and business managers who fail to fulfill their duty of safety measures with 'imprisonment of one year or more' and the Occupational Safety and Health Act. Based on this, various occupational safety and health policies were developed, including the operation of a system related to entrusting the work of safety managers. Despite these efforts, the effect of implementing the Severe Accident Punishment Act is a groundbreaking change in the current disaster prevention policy, which has increased by 0.02%P and 0.03‱P, respectively, from the previous year to 0.65% of the total accident rate and 1.10‱ of the death rate per 10,000 people as of 2022. As the need emerged, attention was paid to 'collaboration and governance with safety management institutions' in the 'Severe Disaster Reduction Roadmap' announced by the Ministry of Employment and Labor in November 2022. In this study, a meaningful result was derived by comparing and analyzing the industrial accident status of workplaces entrusted by “A” safety management institutions with the national average based on the industrial accident survey table, and the types of industrial accidents that occurred in consigned workplaces were selected as intensive management targets. The policy direction for industrial accident prevention was established. It is necessary to develop safety management work manuals based on the results of this study, expertise, discover best cases of risk assessment and develop guides, and educate and train consigned workers. In addition, it suggests that the government's guidance and supervision are needed to advance the professionalism of safety management entrusted tasks, and that safety management institutions should strengthen their roles and functions for preventing and reducing industrial accidents. However, due to difficulties in disclosing information of specialized safety management institutions, the limitation of the provision, collection, and viewing of research-related data to “A” specialized safety management institutions remains a limitation of the research. It seems likely that more thorough research will be conducted.

본 연구에서는 다기준 의사결정분석모델을 사용하여 우리나라 전체 풍력에너지의 잠재량을 연산하였다. 이 모델은 육상과 해상의 풍력터빈 2 MW과 3 MW를 기준설비에 대해 용량밀도 및 이용률을 연산하여 이론적, 기술적 잠재량을 산정한 후 환경규제 및 시장지원정책에 따른 환경성과 경제성과 고려하여 시장잠재량을 산정되는데 풍력 자원, 기술, 그리고 입지환경의 영향단계별 설 비용량 및 발전량을 산정하도록 구성된 잠재량은 최종 의사결정 시나리오를 분석하여 정책적 현안을 고려할 수 있도록 확대 구축하 였다. 의사결정 시나리오의 정책적 현안은 도로 및 주거밀집지역으로 부터의 이격거리를 시나리오로 포함하였다. 전문가 협의에 의 해 도출된 2016년 신재생에너지백서 기준의 풍력잠재량의 발전량 110 TWh/년과 설비용량 39 GW는 도로나 주거건물로부터의 이격 거리에 따라 –33% ~ 8%까지 변화가 있었다. 풍력에너지 보급은 도로와 이격거리 강도에 크게 달라질 수 있으며, 잠재량 변화에 대 한 본 연구 결과는 정부의 보급목표 수립과 적정성을 검토하는데 중요하게 활용될 것이다.

Laver aquaculture, which occupies a large proportion in the aquaculture industry in Korea, is still highly dependent on human labor. Therefore, it is necessary to study the development of an automatic system to improve the working environment and increase the efficiency of aquaculture production systems. The purpose of this study is to evaluate the economic feasibility of an improved system in a study for the loading-unloading and automatic weighing systems in laver aquaculture industry. Economic analysis of the developed unloading and automatic weighing system were implemented under various conditions to calculate more accurate benefits and costs. As a result of this study, the economic feasibility was found to be very high in the three models: net present value (NPV), benefit-cost ratio (B/C), internal rate of return (IRR). Moreover, the results of sensitivity analysis showed that the economical efficiency of the automatic loading, unloading, and weighing system in laver aquaculture was very high.

The swimming behavior of pacific bluefin tuna (Thunnus orientalis) in the offshore sea cage of the brass fishing net was observed and analyzed by imaging sonar techniques. The cultured fish spent most of the time swimming a circular path along the circular cage wall and continued to swim only clockwise direction without completely changing the swimming direction during the 23-hour observation time. In addition, changed swimming behaviors were divided into four categories: (a) the behavior of a large group temporarily swimming in the opposite (counter clockwise) direction, (b) the behavior of a small group temporarily swimming in a small circular path, (c) the behavior swimming small circular path in the center of the cage, and (d) the behavior of a large group swimming across the center of the cage. The maximum swimming speed of the cultured fish was from 3.5 to 3.8 TL/s, the mode was from 1.2 to 1.4 TL/s and the swimming speed during the day time was faster than at night time. It was confirmed the cultured fish swam not only on the surface but also near the bottom net of the cage during the day, but swam mainly at the upper part of the cage at night.

산화적 스트레스는 세포 및 조직 손상을 통해 피부의 탄력 및 보습 기능 저하, 피부 노화 촉진 을 비롯한 다양한 피부질환을 일으킨다. 본 연구의 목적은 인간 피부각질세포 (HaCaT keratinocyte)에서 산화적 스트레스에 대한 붉은 토끼풀 추출물의 효능을 검토하여, 피부에 효과적으로 사용할 수 있는 기능 성 소재로서의 활용 여부를 확인하고자 하였다. 본 연구에서는 붉은 토끼풀 추출물이 인간 피부각질세포에 서 산화적 스트레스에 따른 세포사를 억제시키는 것을 확인하여, 이를 조절하는 보호기전을 규명하였다. 이는 붉은 토끼풀 추출물이 Caspase-3 비활성, 세포사 촉진단백질 Bax 발현 억제, 세포생존 촉진단백질 Bcl-2 발현 증가 및 MAPK 신호전달계 단백질의 인산화 억제를 통해 H2O2에 의해 유도된 산화적 스트레 스를 보호할 수 있다는 것을 확인하였다. 따라서 붉은 토끼풀 추출물은 피부의 산화적 손상을 감소시키는 유용한 소재로 평가되며, 이는 피부보호 및 미용을 위한 다양한 제품 및 산업에 활용 가능성이 높은 것으로 판단된다.

목적 : 본 연구는 무릎관절 치환술 환자를 위한 작업치료 임상 가이드라인을 체계적으로 개발하고자 하였다. 전 세계적으로 무릎관절 치환술을 받은 환자들의 회복과 지역사회 복귀에 있어 작업치료의 역할이 매우 중요하다는 문헌이 보고되고 있으나, 우리나라에서는 관절 치환술 환자를 위한 작업치료가 거의 제공되지 않는다는 현실이 있다. 본 연구에서는 무릎관절 치환술 환자를 위한 근거기반 작업치료 중재를 확인하고 이것을 가이드라인을 통해 제안하고자 한다. 연구방법 : 가이드라인 개발 방법은 영국 작업치료사협회의 ｢Practice guideline development manual(4th edition)｣에서 소개한 절차를 따랐다. Patient/Population, Intervention, Comparison and Outcomes(PICO) 임상 질문 설정은 이해관계자의 의견을 수렴하여 한국의 보건·의료제도를 고려하여 설정하고 이를 바탕으로 체계적 고찰을 진행하였다. 데이터베이스에서 총 7,241건의 논문을 추출하였으며 논문의 질 평가 및 비평적 읽기를 통해 최종 66편의 근거 논문을 선정하였다. 논문의 중재를 바탕으로 가이드라인 내용을 구성하고 권고 강도는 Grading of Recommendations, Assessment, Development, and Evaluations(GRADE) approach 체계를 활용하여 [권고]와 [제안]으로 구분하였다. 결과 : 무릎관절 치환술 환자를 위한 작업치료 임상 가이드라인 권고 내용은 ‘환자 중심’과 ‘서비스 제공 중심’으로 범주화하였다. ‘환자 중심’범주에는 ‘일상생활 활동기능 향상’, ‘정신건강의 안정(불안, 우울 등)’, ‘환자 만족도 증가’, ‘지역사회복귀 향상’을, ‘서비스 제공 중심’범주에는 ‘작업치료사를 포함한 다학제 재활팀 구성’과 ‘작업치료 를 통한 병원 입원 기간 단축’을 위한 내용을 포함하였다. 결론 : 무릎관절 치환술 환자를 위한 작업치료 임상 가이드라인은 수술환자들의 안정적인 지역사회 복귀와 입원 기간 단축에 기여할 것이다. 또한 정형외과 작업치료사 역량 강화를 위한 교육자료로 활용될 수 있으며 의료비 용 감소를 위한 정책 마련에 근거 자료가 될 것이다.

Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria’s early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

Environmental DNA (eDNA) can exist in both intracellular and extracellular forms in natural ecosystems. When targeting harmful cyanobacteria, extracellular eDNA indicates the presence of traces of cyanobacteria, while intracellular eDNA indicates the potential for cyanobacteria to occur. However, identifying the “actual” potential for harmful cyanobacteria to occur is difficult using the existing sediment eDNA analysis method, which uses silica beads and cannot distinguish between these two forms of eDNA. This study analyzes the applicability of a density gradient centrifugation method (Ludox method) that can selectively analyze intracellular eDNA in sediment to overcome the limitations of conventional sediment eDNA analysis. PCR was used to amplify the extracted eDNA based on the two different methods, and the relative amount of gene amplification was compared using electrophoresis and Image J application. While the conventional bead beating method uses sediment as it is to extract eDNA, it is unknown whether the mic gene amplified from eDNA exists in the cyanobacterial cell or only outside of the cell. However, since the Ludox method concentrates the intracellular eDNA of the sediment through filtration and density gradient, only the mic gene present in the cyanobacteria cells could be amplified. Furthermore, the bead beating method can analyze up to 1 g of sediment at a time, whereas the Ludox method can analyze 5 g to 30 g at a time. This gram of sediments makes it possible to search for even a small amount of mic gene that cannot be searched by conventional bead beating method. In this study, the Ludox method secured sufficient intracellular gene concentration and clearly distinguished intracellular and extracellular eDNA, enabling more accurate and detailed potential analysis. By using the Ludox method for environmental RNA expression and next-generation sequencing (NGS) of harmful cyanobacteria in the sediment, it will be possible to analyze the potential more realistically.