The purpose of this study was to evaluate the anti-atopic-dermatitis effect of Angelica keiskei extract using a DNFB-induced animal model of atopic-dermatitis symptoms. A. keiskei was prepared via extraction with DW, 50% ethanol, and 100% ethanol in addition to fresh juice. All the extracts reduced the thickness levels of the ears and ear epidermis against swelling by DNFB inducement, especially in the aqueous-extract-treated group. The mRNA expressions of matrix metalloproteinases (MMPs), however, were not observed, which may indicate that Angelica keiskei extract alleviates the atopic-dermatitis symptoms in an MMP-independent manner. Finally, the level of the inflammatory cytokine IL-4 was inhibited only in the juice-treated group, although the IL-13 level was inhibited in the juice-, 50%-ethanol-extract-, and 100%-ethanol-extract-treated groups in a dose-dependent manner, and was not inhibited in the aqueous-extract-treated group. Taken together, these results suggest that Angelica keiskei extract has an inhibitory effect on atopic dermatitis, and may be a useful biomaterial for the development of cosmeceuticals.

신선초 추출물의 미백활성을 확인하고 이의 소재를 개발하기 위하여, tyrosinase 저해 활성 및 B16F10 세포주를 이용한 melanogenesis 저해능을 확인하였다. 그 결과, 신선초 50% 에탄올 추출물에서 B16F10 melanoma 세포의 멜라닌 생성을 억제하였다. Melanogenesis 억제 활성에 관련이 있는 분자마커의 확인을 위하여 RT-PCR에 의한 mRNA 발현 수준을 검토한 결과, 농도의존적으로 TYR, TYRP-1.

왕겨초액은 최근 목초액을 대신할 수 있는 소재로 많은 연구가 이루어지고 있다. 본 연구는 왕겨초액과 목초액의 항산화 활성을 비교하고, 왕겨초액과 홍삼 및 로즈마리 농축액과의 발효를 통한 시료의 항산화 활성을 비교하여 보았다. DPPH와 FRAP 활성 실험을 통해 항산화 활성을 알아본 결과, 왕겨초액의 항산화 활성은 목초액 보다는 다고 미흡하나, 로즈마리와의 동시 발효를 통해 왕겨초액의 항산화 활성을 높일 수 있으므로, 이취 제거로 인한 기능성 소

화분의 수와 종류에 있어서 차이를 나타내며 K/Na 비율이 3.9-27.9 배로 토종꿀에서 증가한 반면, Fe, Zn, Al은 토종꿀에서 검출되지 않았다. 토종꿀과 양봉꿀은 각각의 구성성분에 있어서 미시적인 차이가 인정되었다. 토종꿀 특이적인 단백질의 전기영동적인 판별과 동시에 본 연구에서 확인한 화분의 종류, K/Na ratio, 및 Fe, Zn, Al의 검출여부 등으로 판별을 확증할 수 있으리라 기대되며 이와 같은 예비적인 특성 비교는 지역의

To evaluate the anti-tumor activity of Flammulina velutipes extract, we used an in vitro wound-healing assay, and an in vivo approach using a mouse melanoma model. Wound-healing activity in B16 cells was affected by the extract in a dose-dependent manner, indicating that the extract had anti-metastatic activity. The extract also exhibited strong anti-tumor activity against lung cancer when B16 cells were injected into mouse veins together with B16 melanoma cells. The results indicatethat the Flammulina velutipes extract decreased B16 cancer cell growth by inhibition of cell migration both in vitro and in vivo.

The experiment was conducted to validate anti-inflammatory effects of pinitol from bean. It was evaluated for some molecule targets by wound healing assay and RT-PCR. The results of wound healing assay was shown dose-dependent inhibition of cell migration in cancer cells and inhibited RNA expression of ICAM-1, CD 44, MMP-17, MMP-14 and ARF2. Immune suppression activity in a mouse provoked by DNFB observed that inflammatory reaction with pinitol were reduced ear swelling and inflammatory cells infiltration in mouse atopic models. The result confirmed that pinitol have the effect of dose-dependent immune suppression activity.