The antioxidant activity and whitening effect of the distilled water (DW) and ethanol extracts of the Prunus persica flower and calyx were studied. In the oxygen radical absorption capacity (ORAC) assay for antioxidant activity measurement, it was confirmed that the flower extract was stronger than the calyx extract, and that the ethanol extract was relatively stronger than the DW extract. To define the whitening effect, an experiment was conducted involving tyrosinase inhibitory assay and measurement of the melanin content of B16F10. As a result of the use of tyrosinase, the DW extract of calyx showed 53% inhibition as the highest activity. The melanin content inhibitory rates were defined as 57% for the ethanol flower extract and 63% of the ethanol calyx extract, based on a 10 μg/mL concentration. Based on these results, mixture with the whitening effect in the extract of P. persica and another compounds should be researched for development as a cosmetic ingredient.

The purpose of this study was to evaluate the anti-atopic-dermatitis effect of Angelica keiskei extract using a DNFB-induced animal model of atopic-dermatitis symptoms. A. keiskei was prepared via extraction with DW, 50% ethanol, and 100% ethanol in addition to fresh juice. All the extracts reduced the thickness levels of the ears and ear epidermis against swelling by DNFB inducement, especially in the aqueous-extract-treated group. The mRNA expressions of matrix metalloproteinases (MMPs), however, were not observed, which may indicate that Angelica keiskei extract alleviates the atopic-dermatitis symptoms in an MMP-independent manner. Finally, the level of the inflammatory cytokine IL-4 was inhibited only in the juice-treated group, although the IL-13 level was inhibited in the juice-, 50%-ethanol-extract-, and 100%-ethanol-extract-treated groups in a dose-dependent manner, and was not inhibited in the aqueous-extract-treated group. Taken together, these results suggest that Angelica keiskei extract has an inhibitory effect on atopic dermatitis, and may be a useful biomaterial for the development of cosmeceuticals.

To investigate the anti-diabetic activity of ethanol and aqueous peel and seed extracts obtained from three different grape species (Cambell Early, MBA, and Kyoho), alpha-glucosidase inhibitory activity was examined. All extracts showed anti-diabetic activity, especially aqueous extract exhibited inhibitory effect above 70%. Thus, we used aqueous extract to check the potential hypoglycemic effects in a streptozotocin (STZ)-induced diabetic mice model. The results showed that the blood glucose level of STZ-induced diabetic mice decreased drastically after 3 hr when the aqueous extract of Cambell Early seed was treated to the mice model. The aqueous extract of Kyoho seed lessened blood glucose level by 60%. Together, these data indicate that extracts of grape peel and seed (aqueous or ethanol) may have potential in improving hypoglycemic effects in the diabetic symptoms, suggesting that further investigation on biomarker expressions should be rewarding.

신선초 추출물의 미백활성을 확인하고 이의 소재를 개발하기 위하여, tyrosinase 저해 활성 및 B16F10 세포주를 이용한 melanogenesis 저해능을 확인하였다. 그 결과, 신선초 50% 에탄올 추출물에서 B16F10 melanoma 세포의 멜라닌 생성을 억제하였다. Melanogenesis 억제 활성에 관련이 있는 분자마커의 확인을 위하여 RT-PCR에 의한 mRNA 발현 수준을 검토한 결과, 농도의존적으로 TYR, TYRP-1.

본 연구에서는 초액 4종인 2차 정제초액 (2nd refined liquid smoke, 2 CV), 3차 정제초액 (3'rd refined chaff liquid smoke, 3 CV), 쑥 정제초액(Artemisia plus refined chaff liquid smoke, A CV), 자소엽 정제초액 (Perillae folium plus refined chaff liquid smoke, Pf CV)의 항산화 능력과 항아토피 효과를 연구하였

In order to compare what kinds of transcription factors are associated with the inhibition of preadipocyte cell proliferation, we prepared several grape extracts and tested the expression patterns by reverse transcription-polymerase chain reaction. As a result, 50% ethanol extract of Campbell early seed inhibited adipogenesis derived from the MDI solution. Extract of Campbell early seed was significantly inhibited lipid droplet formation and expression of molecular factors C/EBP-alpha and delta in 3T3-L1 cells. It is suggested that grape extracts of fractions would be a good candidate for the development of regional skin fat modulator.

자소는 여러 질환을 치료하는 식물로 알려져 있는데, 본 연구는 자소 추출물을 이용하여 항산화 활성 및 항염증 활성을 알아보았다. 자소 추출물을 이용한 DPPH, FRAP 실험 결과, 농도에 따른 항산화 활성을 확인할 수 있었으며, 자소자 추출물의 경우 고온/고압 추출시 활성이 증가하는 것을 알 수 있었다. 마우스 동물모델을 이용한 항아토피활성 결과 귀와 상피의 비후를 감소시키고, 면역세포의 침투현상을 억제하는 것으로 나타났다. 이상의 결과로, 자

나비류 추출물은 전통적으로 다양한 활성을 보유하여 의약용으로 사용되어졌다. 5종의 나비(산제비나비[Papilio maackii], 호랑나비[Papilio xuthus], 배추흰나비[Pieris rapae], 남방호랑나비[Eurema hecabe], 왕오색나비[Sasakia charonda])를 이용하여 물, dimethly sulfoxide (DMSO), ehtanol 및 methanol로 추출한 추출물로 항산화 활성 및 Cox-2 promote

화분의 수와 종류에 있어서 차이를 나타내며 K/Na 비율이 3.9-27.9 배로 토종꿀에서 증가한 반면, Fe, Zn, Al은 토종꿀에서 검출되지 않았다. 토종꿀과 양봉꿀은 각각의 구성성분에 있어서 미시적인 차이가 인정되었다. 토종꿀 특이적인 단백질의 전기영동적인 판별과 동시에 본 연구에서 확인한 화분의 종류, K/Na ratio, 및 Fe, Zn, Al의 검출여부 등으로 판별을 확증할 수 있으리라 기대되며 이와 같은 예비적인 특성 비교는 지역의