본 연구는 노지채소 작물인 고추를 대상으로 이상 고온조건을 인위적으로 부여하여 화분발아력을 검정 하며, 이상 고온에서의 화분발아력을 향상시킬 수 있는 방법을 강구하기 위해 수행되었다. 고추에서 화 분 발아에 최적온도는 25℃였고, 27.5℃와 30℃에서는 화분발아율이 낮았다. 화분발아에 적합한 sucrose 농도는 10~15%였고, 30% 이상의 농도에서는 화분 발아율이 감소하였다. 화분발아 배지의 적정 pH 5.5 ~6.5였고 이들 조건에서 화분발아율이 높았고, 화분관 신장도 좋았다. 화분 채취시간에 따른 화 분 발아율은 채취 시간에 따라 차이가 있었다. 전반적으로 오전 10시에 채취한 화분에서 발아율이 가장 높았고, 반면 오후에 채취한 화분일수록 화분활력이 저하되었다. 화분발아 배지에 아미노산인 asparagine과 glutamine를 첨가하면 고온에서 화분발아율이 향상되었다. 또한 폴리아민류인 putrescine 과 spermine도 화분 발아율을 향상시켰다. 전반적으로 고온에서 고추 화분발아성을 증진시킬 수 있는 첨가물질 및 처리농도는 supermine 1 mM 처리였고, 대조구에 비해 화분발아율이 15.0% 향상되었다.

본 연구는 국내 주요 과학관 및 자연사 박물관의 지구과학 관련 전시 라벨 텍스트의 서술 특징을 분석하고자 하였다. 이를 위해, 국립 중앙 과학관과 서대문 자연사 박물관에서 자료를 수집하였고, 체계기능언어학을 일부 적용한 분석틀을 사용하였다. 연구 결과, 라벨들은 1) 대부분 평서형 문장, 2) 적절한 과학적 정보의 양, 3) '사실' 위주의 정보 제시, 4) 모두 논리적 설명 장르로 구성되어 있었다. 특히, 국립 중앙 과학관의 라벨들은 5) 전체 어휘 중 과학 용어의 비율이 높고 6) 주어의 절반 이상이 생략되거나 긴 명사화 형태였다. 결론적으로, 분석 대상 라벨들은 전시물과 관람객들 사이에서 과학 문화에 관한 양 방향적 의사소통을 이끌기보다 일 방향적인 의사소통을 일으킬 가능성이 높은 것으로 해석할 수 있었다. 이를 토대로, 과학 문화와 관람객의 일상 문화 사이의 열린 구조를 만들어 보다 교육적으로 유의미한 의사소통을 가능하게 하는 라벨 텍스트의 서술 특징들을 제안하였다.

매년 개최되는 '과학 축전'에서는 부스 운영 학생들의 안내 하에 다양한 과학 및 기술관련 워크숍, 시범 실험등의 활동이 제공된다. 본 연구는 과학축전에 참여한 부스 운영 학생들과 참여 관람 학생들의 인식을 탐색한 것이다. 연구대상은 6일 동안 축전에 참여한 부스 운영 학생 323명과 참여 관람 학생 495명이다. 본 연구에서 사용한 설문지는 안광호와 박일우(2009) 및 이선경 외(2010)의 설문 문항을 수정 보완하여 완성되었다. 연구 결과로서, 학생들의 참여 배경, 참여 유형별 및 학교급별 교육적 경험에 대한 인식, 부스 운영 학생들의 과학탐구 경험에 대한 인식 등을 살펴보았다. 연구 결과는 과학축전을 준비하는 과정에서 부스 운영 학생들이 문제를 제기하고 해결하는 탐구 과정에 참여한 것을 의미 있는 경험으로 인식했다는 것을 보여주었다. 연구의 결과를 토대로, 비형식 과학교육으로서 공동체 기반 프로그램에 대한 의미와 시사점을 제시하였다.

탐구는 과학교육의 본질을 설명해주는 결정적인 특징이다. 최근에는 스스로 문제를 제기하여 탐구하고 그것을 의사 소통하는 능력을 포함한 논증이 강조되고 있는데, 학교 탐구학습 맥락에서는 자율적이고 활발한 상호작용의 측면에서 그 한계가 지적되고 있다. 그래서 대안으로 비형식 맥락에서의 탐구학습이 제안되고 있으며, 이에 본 연구는 국립과천과학관 내 자연사관을 자유 관람하는 중학생 소집단의 대화적 탐구에서 나타나는 상호작용 유형을 밝히고자 하였다. 이를 위해 중학생 13그룹의 탐구적 대화 42사례를 대상으로 탐구요소 및 상호작용 유형을 분석하였다. 탐구요소는 문제제기, 자료수집, 설명의 세 가지로 구분하였고, 상호작용은 대화의 흐름을 화살표로 도식화하여 나타내었다. 문제제기로부터 탐구가 시작된다고 보았으며, 문제제기 이후 후속적인 반응에 따라 유형이 구분되었는데, 총 7가지 유형이 발견되었다. 그 유형은 문제제기 공유, 문제제기-단순응답, 문제제기-단순설명, 문제제기-단순설명-(자료수집)-자료기반 설명, 문제제기-자료수집-자료기반 설명의 5가지로 크게 나누어지고, 가장 많은 빈도를 보인 유형 2는 다시 소극 회피적 응답, 부정확한 응답, 패턴의 반복으로 세분화되었다. 본 연구는 비형식 탐구학습에서 학교 탐구학습과 차별화되는 상호작용 패턴을 파악하였다는 점에서 기존 연구들과 차별성을 가지며, 비형식 탐구학습에서 상호작용 유형을 기술하고 있다는 점에서 비형식 탐구학습 연구를 위한 기초를 제공하였다는 의의를 가진다.

This study was conducted to investigate changes in seed vigor based on temperature of dry heat and duration treatment of watermelon seeds and examine the effect on percent of emergence and seedling vigor. When the upper limit temperature of dry heat treatment was raised to 80℃, the percent of the germination decreased. Moreover, T50 was delayed as the upper limit temperature of dry heat treatment increased. The higher the upper limit temperature of dry heat treatment and the longer the treatment period, the higher the percentage of abnormal seedlings. The optimum upper limit temperature for dry heat treatment was 72℃, and the treatment period was five days. Seed vigor was better maintained at 30℃, 45℃, and 52℃, followed by stepwise exposure to high temperatures of 72℃, the upper limit of dry heat treatment, rather than dry heat treatment at a high temperature of 72℃ for 5 days from the initial stage of treatment. When the fungicide was added during the dry heat treatment process, the germination percentage decreased and the percent of the abnormal seedling percentage increased. However, the addition of 10 mg/kg fungicide did not significantly reduce seed vigor.

This study investigated the applicability of horticultural media with recycled coir substrates the growth of Chinese cabbage (Brassica campestris L. ssp. Pekinensis) and lettuce (Lactuca sativa L.) crop. The six different types of coir based substrates were A, Coir 45: Perlite 35: Vermiculite 12: Zeolite 8 (%), B, Coir 55: Perlite 25: Vermiculite 12: Zeolite 8 (%), C, Coir 65: Perlite 15: Vermiculite 12: Zeolite 8 (%), D, Coir 75: Perlite 5: Vermiculite 12: Zeolite 8 (%), E, Coir 85: Perlite 5: Vermiculite 5: Zeolite 5 (%) and F, nursery media (control). The pH and Electric conductivity of the horticultural nursery media were 6.06–7.00 and 0.45–1.10 dS/m-1, respectively. The nursery media containing coir substrates had higher level of Total N, Ca, K, Mg and P than those without coir. Additionally, it was observed that the growth of Chinese cabbage was the best on D (containing 75% coir) while that of lettuce was the best on E (containing 85% coir). In general, when substrates containg a higher percentage of coir were used, the growth of Chinese cabbage and lettuce was ideal. Additionally, the P, Ca, and Mg content in both plants was not significantly altered by the amount of coir present in the media. However, with an increase in the amount of coir substrate, the chlorophyll, N, and K content was increased. After harvesting, there was no significant difference in the chemical properties of the horticultural nursery media of both plants. Thus, it can be suggested that, coir substrate after a single use could be recycled as horticulture nursery media.

The present study aimed to investigate the effects of low temperature on the growth, yield, quality, and biologically active compounds of strawberry and obtain basic information for developing a technology for stable growth of strawberry in greenhouses. Growth of strawberry, including leaf number, area, and length, plant height, and dry weight was better at the optimum growth temperature of 20℃ than at a lower temperature of 15°C. At the low temperature of 15°C, the cultivar 'Maehyang' was more tolerant and displayed better growth rate than 'Seolhyang'. At 15°C, the fruit production per week and fruit weight was lower than that at 20°C. In contrast, fruit length and diameter were not significantly different between the two growth temperatures. Growth temperature also did not affect the fruit color index, Hunter L, a, b value, or fruit firmness. However, the sugar content of strawberries grown at 15 was higher by 0.8 and 1.5 Brix for 'Seolhyang' and 'Maehyang', respectively, than of those grown at 20°C. There was no difference in the content of fisetin, a biologically active compound, for 'Seolhyang' at both growth temperatures, however, the fisetin content of 'Maehyang' was higher at 20°C than at 15 . Cinchonine and ellagic acid content of 'Seolhyang' was higher at 20°C than at 15 , whereas that of 'Maehyang' was higher at 15°C than at 20℃ . Quercetin content showed no significant differences with respect to growth temperature, however, it tended to increase at 20°C. The cinnamic acid content of 'Seolhyang' was higher at 15°C than at 20℃ , whereas that of 'Maehyang' increased at 20°C. Collectively, the biologically active compounds of strawberry were affected by growth temperature. Moreover, the content of these compounds tended to increase at 20°C, the optimum growth temperature, rather than at the sub-optimal growth temperature of 15°C.

In the present study, we investigated the effects of artificial removal of corolla and calyx lobes from open flowers on fruit development and fruit quality of strawberry (Fragaria × ananassa Duch.). Removing the corolla had no significant effect on fruit weight, hardness, length, and diameter, Hunter L, a, b values, sugar content and acidity. However, removing the calyx lobes significantly inhibited longitudinal and transverse growth of the fruits, resulting in diminished fruit size. In addition, merchantable fruit quality significantly decreased upon removing the calyx lobes, and this tendency was increased as more calyx lobes were removed. Nonetheless, removal of the calyx lobes had no effects on fruit color, sugar content, or acidity, similar to the effect of corolla removal.

The present study was investigated the effect of recycled coir organic substrates on the growth of different vegetable crops. The recycled coir had better physical and chemical properties than the new coir. The growth of tomato plant was better on the coir substrate that had been used for 2 years than that on the new coir substrate. The average number of tomato fruits was 108 on the new coir substrate, while it was 179 and 165 on the coir substrate used for 1 and 2 years, respectively. The growth of cherry tomato plant was also better on the coir substrate used for 2 years than that on the new coir substrate. The average number of cherry tomato fruits was 43 on the new coir substrate, while it was 206 and 164 on the coir substrate used for 1 and 2 years, respectively. The growth of brussel sprout was better on the coir substrate used for 3 years than that on the new coir substrate and the average number of brussel sprout leaves was 26.8 on the new coir substrate, while it was 34.3 on the coir substrate used for 3 years. The growth of Korean cabbage improved on the coir substrate used for 1 years compared to the new coir substrate and the number of leaves was 15.1 on the new coir substrate, while it was 24.3 on the coir substrate used for 1 year. Thus, used coir can be recycled to improve vegetable yields compared to using new coirs.

In nine commercial watermelon cultivars, the effects of osmotic seed priming were evaluated for seed viability and seedling growth at different germination temperatures and field conditions. Generally, primed seeds showed improved germination rate and reduced time for emergence at different germination temperatures, as compared to untreated seeds. In particular, priming effect on seed germination was significantly high at 15℃, which is an environment normally resulting in poor germination. Depending on the watermelon cultivar, germination rate under low temperature (15℃) was improved by up to 15~66% in primed seeds, as compared to untreated seeds. However, priming effect on germination was gradually reduced as the temperature reached to its optimum level for seed germination. Seed priming tended to improve the viability, fresh weight, and dry weight of watermelon seedlings, but its effect on seedling stage was not large, as compared to untreated seeds. Primed watermelon seeds showed improved emergence rate and facilitated germination in the field, but their seedling growth after 30 days from germination was not significantly affected. Our results indicated that seed priming can greatly improve the seed germination at poor temperature conditions in the watermelon.

This study was conducted to investigate days after anthesis (DAA) and fruit after-ripening period (FAP) for seed-harvesting of high quality watermelon seeds. Fruit weight and number of seed per fruit increased according to DAA, while those did not significant about FAP. Ratio of cotyledon at whole seed was higher about 2 to 4% compared to seed coat irrespective of DAA and FAP. Germinability of watermelon was not a significant effect by DAA, however, it had differences by FAP. Percent of germination (PB) was below 50%, when 30 days maturated fruits after anthesis was omitted ripening, while PB was increased to 92% by ripening. In addition, seeds at DAA 40 and FAP 20 were higher general seedling vigors (hypocotyl length, diameter etc.) in BP test. Results indicated that considering seed productivity, it had maximized seed viability at DAA 40 and FAP 20.

This study was conducted to investigate the effects of fruit set internode on the germination of watermelon seeds. Generally fruits setted in higher than 20 internode were high percent of germination, fruit setted in low internode(5-10) is low. The higher fruit-set internode, fruit weight was significantly increased, but 1,000 seed weight was decreased the more fruit set internode. However number of seed was about 300 seeds irrespective of fruit-set internode. Investigating viability of seed by BP test, general percent germination was recorded highest at 5 flower cluster above 20 internode of fruit-set internode. In contrast, condition of fruit setted on 3, 4, 6 flower cluster were high percent of germination, regarded as unnormal germination not to be as normal seedling by 2~3%. Seed harvested at 5 flower-cluster had high viability on hypocotyl height and diameter of seedling through early growth test, but were not significant. Therefore 5 flower-cluster was optimum fruit-set internode to obtain high-quality seed.

The present study was conducted to find a way to recycle the coir substrate by investigating changes in its physical and chemical properties based on the number of use year. Specific gravity of unused coir substrate was 0.212 g/cm3 , while it was higher for the substrate used for 2 years. Porosity was different depending on the number of use year. The porosity of unused substrate was 51.9%, but it increased to 68.6% after used for 2 years. In general, physical and chemical properties were better in the coir substrate used for 2 years than in unused one. The number of leaves, leaf area, flesh weight and dry weight of oriental cabbage and lettuce were higher in coir substrate used for 2 years than those in unused one. Whereas, no significant difference was observed between the substrates used for one year and 2 years, indicating that the one time-used wast substrate could be recycled for cultivating vegetables. Growth of the vegetables was improved when organic fertilizer composed of complex organics with different mixing ratios was provided to the coir substrate, compared to untreated plot. The optimum mixing ratio of the wast substrate and complex organics was 2:8(v/v) for fertilization using wast coir substrate. Therefore, coir substrate generally wasted after being used for one time was reuseable by supplying organic fertilizer.

시들음병은 진균성 병원균인 Fusarium oxysporum f. sp. conglutinans에 의해 양배추(Brassica oleracea var. capitata)에 발병하는 심각한 병해이다. 최근, 양배추의 시들음병 저항성 유전자, FocBo1, 과 연관된 분자 마커 (MTK-C)에 대한 보고가 있었다. 본 연구에서는, MTK-C를 이용하여, 국내 시들음병 저항성 육종을 위한 양배추 육종 계통이 FocBo1 유전자와 연관된 것인지 판별하고자 하였다. 상업적으로 판매되고 있는 ‘대박나’를 대조군으로 하여 6가지의 양배추 육종계통을 통제된 실험실 조건에서, 시들음병을 처리하고, 생물검정에 따른 발병도와 식물체내 병원균의 생체량을 확인하여 육종계통의 시들음병에 대한 반응성을 정확히 평가 하였다. 그결과, KR-518, OK-517은 반복 실험을 통해 병징 뿐만 아니라 병원균의 바이오 매스도 거의 검출되지 않는 강한 저항성을 나타냈고, RK-P6-1, MT-624는 실험실내 병 처리 환경에 따라 그 저항성과 감수성의 정도가 달라지는 각각 중도 저항성, 감수성으로 분류 할 수 있으며, JK-2, HY-164는 병징과 바이오매스 모두에서 병에 대한 감수성을 보임을 확인 하였다. FocBo1 간의 상관관계를 알기 위하여, 6개 양배추 계통의 genomic DNA를 추출하고, MTK-C 마커를 이용하여 PCR을 수행한 결과, 흥미롭게도 감수성 유전자원인 HY-164 식물체에서도 FocBo1 밴드가 관찰 되었다. 염기서열의 차이를 확인하고자 증폭된 밴드를 클로닝하여 sequencing 한 결과 감수성 HY-164와 저항성 KR-518 간의 차이는 없었다. 하지만, HY-164의 식물체에서는 FocBo1 유전자의 발현이 없는 것을 cDNA를 이용한 RT-PCR 결과로 확인 하였으며, 더하여, FocBo1 유전자의 발현 정도가 완전 저항성, 중도 저항성을 결정하는 중요 요인이라는 것을 실험을 통해 확인 할 수 있었다. 더하여, 확인된 MTK-C 밴드의 염기서열을 이용하여 단일밴드로 정확히 FocBo1 발현을 확인할 수 있는 프라이머, FocBo1-C를 만들 수 있었다. 본 실험을 통하여, 국내에서 보유한 6개 시들음병 관련 양배추 육종계통의 정확한 병 반응성을 정리 하였으며, 또한 이들의 시들음병 저항성이 FocBo1 유전자 및 발현과 밀접한 관련이 있다는 것을 확인할 수 있었다. 따라서, FocBo1을 이용한 분자육종에서 저항성과 감수성 판별을 위하여 genome 상의 FocBo1 존재뿐만 아니라 그 발현의 정도도 같이 확인 하여야 한다는 것을 확인하였다.

The present study was conducted to development mass production methods for peanut sprouts that is considered as a field of blue ocean among the agricultural products. 'Jopyeong' was the best as a major cultivar for peanut sprouts production. The manual for the production of high-quality peanut sprouts is as following. Germination temperature appropriate for production of high-quality peanut sprouts was 27℃. Peanut sprouts at the growth stage of 8th day, and older plants with advanced growth showed deteriorated merchantable and eating quality. Resveratrol compound was not found in the seeds, but its highest amount was detected from 9-day old sprouts. The best water temperature applicable to high quality peanut sprout production was 25℃. The growth of peanut sprout was inhibited by the high temperatures above 35℃ and low temperatures below 15℃.

The present study was conducted to develop seed treatment for the production of healthy and clean peanut sprout. Dry heat treatment of peanut seeds reduced the incidence of the rot. The seed treatment condition at 52℃ for 10 h. was the most efficient without inhibiting seed viability significantly. Seeds were dark cultured at 27℃ for up to 9 days. The treatment of Indole-B and gibberellic acid influenced germination, T50, fresh, dry weight, hypocotyl length, hypocotyl length diameter, root length, number of lateral root and epicotyl of peanut sprout. There were no differences in the germinability of peanut seeds between gibberellic acid treatment methods but higher fresh weight was observed in the GA3 solution spray after 2 hour water soaking. The general growth and lateral root development of peanut sprouts were suppressed by Indole-B which is used for inhibiting root formation and promoting hypocotyls. The treatment of gibberellic acid promoted hypocotyl elongation, but it did not influence on the growth of hypocotyls and root system.