Background: The current regulations regarding the storage of medicinal herbs do not define the storage temperature; instead, but the only requirement is that the plastic bags used for storing the medicinal herbs should be tightly sealed during storage. The practice of storing medicinal herbs at room temperature (28℃), causes many problems, including growth of insect pests on the stored medicinal herbs. Thus, it is necessary to understand the effect of insect pests on the quality of medicinal herbs stored at room temperature (28℃) for the improving the relevant regulations. Methods and Results: Cnidium rhizome and Angelica radix were infested with Lasioderma serricorne F. adults and incubated at 28℃ for 2.5 and 5 months. The population of L. serricorne was established rapidly, and left many holes, cadavers, and feces on the stored medicinal herbs, thereby greatly damaging the product appearance and hygiene. In addition, active ingredients of the medicinal herbs were significantly decreased, probably reducing their medicinal quality as well. Conclusions: These results indicate that the current regulations are not sufficient to prevent the establishment of storage insect pests and guarantee the quality and hygiene of stored medicinal herbs. Therefore, it is necessary to devise proper storage protocols and upgrade the current relevant regulations to maintain the quality of medicinal herbs during storage.

Background : The practice of keeping the medicinal herbs at room temperature causes many problems, but due to lack of sufficient field research and study, it is difficult to improve related regulations and safety management. Methods and Results : The Cnidium Rhizome and Angelica gigas Root were inoculated with Lasioderma serricorne F. and incubated at 28℃ for 2.5 months and 5 months. After five months, the number of Lasioderma serricorne F. in Cnidium Rhizome increased from 30 to 1,429 (about 47 times). In the same period, the number of insects in Angelica gigas Root increased from 30 to 663 (about 22 times). Due to the rapid increase in pest population, hygiene deteriorated, changes in the active ingredient and appearance quality of the herbal medicines, which greatly damaged the value of the herbal medicine. Conclusions : These results show that current regulation requiring only sealing and not specifying the storage temperature do not guarantee quality safety. Therefore, it is necessary to establish appropriate preservation standards and improve management regulations in order to preserve safety.

Background : This experiment was carried out to investigate the occurrence of disease and pest and the yield depending on cultivation methods in Cynanchum wilfordii. Methods and Results : There were damages by anthracnose, aphid, mite, thrips, Gastrophysa atrocyanea, Tropidothorax cruciger and Metcalfa pruinosa. In the case of anthracnose, the number of damaged leaf in the untreated control plot per counting area (67.5 ㎡) was 8.6 times higher than that of chemical treated plot. As a result of investigation of shoot damage and leaf juice damage by Tropidothorax cruciger, which made the most obvious damage symptom, showed the significantly lower degree of damage in the treatments of insecticides (abamectin, suloxac flour liquid wettable powder). In addition to the pest damage, the yield of roots was investigated. As a result, it was found that the yields were 3.8 ㎏, 2.6 ㎏, 1.7 ㎏ and 1.4 ㎏ per 8.5 ㎡ respectively in vine scheme cultivation with chemical control, vine scheme cultivation with non-chemical control, non-vine scheme cultivation with chemical control and non-vine scheme cultivation with non-chemical control. Conclusion : The yield per 1,000 ㎡ was 449 ㎏, which was 1.9 times higher than the national average of three years in vine scheme cultivation with chemical control.

Backgoound : This study was conducted to serve as a basis for the selection of superior lines by analyzing the content of antioxidant component and antioxidant activity in Schisandra chinensis Collections Methods and Results : In order to examine antioxidant component and antioxidant activity, 154 species of Schisandra chinensis from whole country were used. Antioxidant component was investigated by total flavonoid content and total phenolic content and antioxidant activity was evaluated by DPPH radical scavenging activity and ABTS radical scavenging activity. As a result, the total amounts of flavonoids was highest in SC-20 with 5.03 ㎎/g. However the content of polyphenols showed highest in SC-22 with 2.76 ㎎/g. In addition antioxidant activity results were also relatively high in SC-22. The IC50 value was 548 ㎍/㎖ in DPPH radical scavenging and 640 ㎍/㎖. in ABTS+ radical scavenging. Conclusion : As demand for high income crop has increased, new cultivar breeding is required to produce high quality Schisandra chinensis From this study, analyses of antioxidant component and antioxidant activity in collection can be used for new Schisandra chinensis breeding. Especially SC-22 can be superior lines with high antioxidant component and antioxidant activity.

Background : This study aimed to determine the use of proper organic fertilizer through side dressing application in the environmental cultivation of Adenophora triphylla var. japonica Hara. to improve farming income. Methods and Results : In the cultivation of eco-friendly and environmental medicinal plant like Adenophora triphylla var. japonica Hara., it is necessary to use organic instead of chemical fertilizer. Organic fertilizers such as HL, CK, OK were applied three (3) times during the last ten days of July, August, and September. There was no significant difference between CF and NT, in terms of the number of rooting per 1㎡ which was 12.1 -12.9. There were also no significant differences in plant length, leaf length, leaf width and dry weight. The root length was 19.9 ㎝, which was the longest in CK, and 16.8 ㎝, the shortest in ST, but described as no significant difference. The root width was 14.8 – 5.5 ㎜, and the number of rootlet was 5.7 - 7.1, but was considered not a significant difference. The dry weight was 16.6 g in HL, 16.3 g in CK and ST, and 15.6 g in OK, but still there were no significant differences observed. However, it was 14.6 g in NT, which was considered the lowest, thus there was a significant difference. The number of dry root per 10 a was 149 ㎏ which is considered the most in HL, 141 ㎏ in CK, 140 ㎏ in ST, and 138kg in OK yet there were no significant differences observed. While NT only had 123 ㎏ hence, there was a significant difference on the number of dry root per 10a as compared with the group applied with the three organic fertilizers. Conclusion : In conclusion, it is proper to use Organic Fertilizer HL and CK through side dressing application in growing Adenophora triphylla var. japonica Hara.

Background : This project was conducted to determine proper seed harvest time and seed storage methods of Adenophora triphylla var. japonica Hara. Methods and Results : When Adenophora triphylla var. japonica Hara. transplanted this year, seeding can only be done the following year. Therefore, the seeds harvested in the interval of ten (10) days were investigated for seed harvest amount, germination rate, seed storage temperature and storing on container, between the period late August and mid-October. It is when the seed fructification started and ended. The seeds harvested from the first ten (10) days of October to mid October were 87.9% in whole seeds. The proper seed harvesting time was mid October which has a germination rate of 75% in whole seed harvest time. The lowest germination rate of 27.3% was at the germination temperature of 15℃. As the temperature rises, the germination rate increases. In 29℃, the germination rate was at 79% which is the highest; and from 30℃, the germination rate decreased to 71%. When the storage temperature was at 0℃ after storage of 5 months, the germination rate was at 83%. However, after storage of 12 months, it decreased by 7% that was 76%. When in 4℃, after storage of 5 months the germination rate was at 85%. However, after storage of 12 months it decreased by 8% which was 77%. In room temperature after storage of 5 months, germination rate was at 77%, but after storage of 12 months it decreased by 22% which was 55%. When seeds were stored in vinyl container, it was observed that the germination rate was at 86% after 5 months of storage, which was 9% higher than seed stored in paper which was 77%. After 12 months storage, seed in vinyl container had a germination rate of 71%, which was 3% higher than seed in paper at 68%. Conclusion : It is concluded that the proper time of seed gathering of Adenophora triphylla var. japonica Hara. is during the first ten (10) days of October to mid-October. When the long time storage is needed, below zero temperature with vinyl container is more appropriate.

Background: The roots of Atractylodes macrocephala Koidzumi contain atractylone, which is used to suppress appetite and indigestion caused by gastrointestinal disturbance. The present study was conducted to investigate the effect of several organic compost on the growth and root yield of A. macrocephala with organic fertilizer.Methods and Results:When organic fertilizer was applied basally, the average yield of 10 a was 184.6 ㎏ in the HA (Hwanggeumjidae, organic material mix), 171.3 ㎏ in the GG (Gyunbaeyangchegreen, bacterial cuture filtrate) and 175.0 ㎏ in the CF (Customary fertilization, control) each other in practice of CF had no statistical significance. Atractylenolide I was significantly greater in the HA (0.036%) than the GG (0.034%) or CF (0.023%). With regard to the amount of organic fertilzer, 10 a yield ws the most common of 203.0 ㎏ at 2.0 times of the organic 1 (HA), conventional fertilization of 134.0 ㎏ and 173.0 ㎏ of no application was a statistically significant. Organic fertilizer 1 was 1.5 to 2.0 times, organic fertilizer was 2 to 1.5 times that were most suitable.Conclusions:The results of the present study indicated that HA and GG are the most suitable for the organic cultivation of A. macrocephala. The content of atractylone I was highest under the HA treatment and lowest under the CU (Chamjoa, oil cake), TG (Totogreen, plant oil cake) and HG (Heuksalgreen, Castor oil cake) treatment.

Background : Recently, with the growth of traditional medicine industry, management of the quality of herbal medications as a raw material for functional food and medicine becomes more important. One of the most difficult problems of quality control of medicinal plants is management in storage insects. The problem of storage insects is a matter that must be resolved in order to safely obtain raw materials in herbal sector. But the basic prevention measures as well as grasp of the exact situation in the field are not yet. In this study, we investigated to see the problems and solutions through a review of storage pest management system of medicinal plants and the relevant provisions in herbal sector. Methods and Results : For this study, we examined the Status of domestic distribution, storage pest control techniques, news, domestic and import regulations. Storage pests that occur in herbal medications have dropped commercial value and it leads to consumer mistrust. A report said that consumers who had found at least once a pest and microbial contamination when they buy a herbal medications is more than 50% (2004, Ministry of Food and Drug Safety). In the past, those who want to solve these problems had tried through the sulfur fumigation. However, due to concerns about the risk to humans nowadays it was banned in many countries. According to the latest editions of Korean Pharmacopoeia (KP), it is allowed to use a suitable fumigant. but the domestic and imported medicinal plants during distribution can not be used it. Because currently registered fumigant (aluminium phosphide) is allowed only imported medicinal plants in the quarantine process. Chemical fumigation is inappropriate for use in foods and pharmaceuticals so the introduction of eco-friendly control methods is urgent. Eco-friendly methods of pest control that is currently being developed include a cold storage, packaging, high temperature and frozen method etc. Conclusion : These methods should be considered potential cost and Feasibility and it should meet the condition that stated “"Do not change the therapeutic effects of the herbal medications.” in the KP. Also supporting policy for expanding low temperature storage facilities in distribution area will raise possibility of success.

Background : Most of cases in korea, distributed herbal medications are storaged in room temperature and it causes many insect pests during storage. Despite the fact, there is little research about storage conditions. Temperature and humidity in storehouse are highly related with pests’ overwintering, generation length and the number of occurrence in summer. Consequently, in this research, major pests’ overwintering was studied by reviewing facility condition, temperature and humidity in major regional storehouses. The results of this would help to develop how to manage pest occurrence in distributed herbal medications. Methods and Results : For this study, storage facilities was investigated at different regions including Gyeongdong Market in Seoul, the pharmaceutical company in Daegu and farmhouse in Youngcheon by monitering temperature, humidity, ventilator, air conditioning and heating on ground and underground places from June 2014 to July 2016. including Upon investigation, most of building using for storage had simple ventilators. Whereas in case of farmhouse, it is vulnerable to maintaining quality. Because most farmhouse had temporary buildings which are difficult to control temperature and humidity. In addition, among the other surveyed warehouses, Basement in the Gyeongdong Market had most favorable condition for pest overwintering. The lowest temperature of it was seldom below zero maintaining level of about 1.5℃ and average temperature was about 10℃ in underground warehouse during winter. And the ground warehouse of Daegu had similar patterns in temperature and humidity with that of Seoul in that being over 0℃ even in the winter. In Youngcheon, the temperature of farmhouse was below zero degree and the daily range of temperature and humidity was wide. It caused difficulty of overwintering of insect pests but possible to inhabit. Conclusion : According to a study by Han-Mo, Koo(1997), it was impossible to overwinter for Lasioderma serricorne F. which is dominant medicinal plants’ pest in north of the Chungcheong region’s warehouse due to low temperature. But in case of investigated warehouses, it was possible to overwinter because of the high temperature in office building or basement storage area. In order to make prediction of pest occurrence and comprehensive measures, it is urgent to take more investigation by regional groups and types of storage.

Background : In previous studies, adlay seeds showed a prevalence of diversified fungal flora with the predominant fungal genera being Fusarium (45.6%) and In vitro test showed that fungal toxins like Fumonisin and Zearalenone were produced by Fusarium fujikuroi, F. asiaticum and F. graminearum. Because of this we performed experiments to selecting disinfective chemicals for controlling the Fusarium contamination in the adlay seed. Methods and Results : We carried out the chemical efficacy test such as seed disinfectants selection test appling before planting and pesticides selection test using in the earing season. In the present study, eleven different commercially available seed disinfectant were applied to the adlay seeds. Among 11 seed disinfectants, Hexaconazole+Prochloraz emulsifiable concentrate (EC) and Prochloraz emulsifiable concentrate (EC) had control value of 80% or above against Fusarium species tested. In the pesticides selection test, seven different commercially available pesticides for Fusarium blight (Scab) control were applied and we observed that Metconazole suspension concentrate(SC) strongly inhibited the mycelial growth of 10 Fusarium species all. Conclusion : From the above results, we selected Hexaconazole+Prochloraz EC and Prochloraz EC as a seed disinfectants and Metconazole SC as a pesticide using in the earing season for Scab control.

Background : This experiment was conducted to select GAP applying seed disinfectants in Astragalus membranaceus and Platycodon grandiflorum. Methods and Results : We carried out the chemical efficacy and injury test. For the efficacy test, we investigated fungal detection rate by seed disinfectants and for the chemical injury, we investigated germination rate and emergence rate by seed disinfectants in reference amount and fold amount. These experiments carried out two times. The results obtained are as follows. In the experiment for seed disinfectants selection of Astragalus membranaceus, all tested chemicals such as Tebuconazole emulsifiable concentrate(EC), Thiophanate-methyl + Triflumizole wettable powder(WP), Prochloraz copper chloride complex+Tebuconazole suspension concentrate(SC), Prochloraz emulsifiable concentrate(EC), Fludioxonil wetting liquid(WL) and Hexaconazol+Prochloraz emulsifiable concentrate(EC) had control value of 80% or above against seed contaminated fungi. However two chemicals such as Tebuconazole EC and Prochloraz copper chloride complex+Tebuconazole SC and two chemicals such as Prochloraz EC and Hexaconazol+Prochloraz EC exhibited chemical injury significantly in reference amount and in fold amount respectively, compared to non treated control. In the case of seed disinfectants selection of Platycodon grandiflorum, Prochloraz copper chloride complex+Tebuconazole SC, Prochloraz EC and Hexaconazol+Prochloraz EC had control value of above 80% against seed contaminated fungi except Thiophanate-methyl+Triflumizole WP and Fludioxonil WL. However Hexaconazol+Prochloraz EC and Prochloraz copper chloride complex+Tebuconazole SC exhibited chemical injury significantly in reference amount and in fold amount respectively, compared to non treated control. Conclusion : From the above results, we finally selected three items of seed disinfectants including Thiophanate-methyl+Triflumizole WP and Fludioxonil WL in Astragalus membranaceus and Prochloraz emulsion in Platycodon grandiflorum.

Background : Adenophora triphylla var. japonica Hara. is one of the important oriental medicinal herbs being used to cure lung/respiratory diseases. It belongs to the five Sam (ginseng) including Panax ginseng, Salvia miltiorrhiza, Sophora flavescens, and Scrophularia buergeriana. In 2015, Korea produced 72 tons of medicinal herbs in a 14-hectare area and imported 24 tons valued at US$95,000. This study was carried out to investigate the effect of several biopesticides to control leaf spot of A. triphylla var. japonica Hara. Methods and Results : One year plants at the Department of Herbal Crop Research, NIHHS, RDA were used. The treatments used biopesticides such as plant extracts (BP), microbial agent (DM), microbial agent (PJ), sulfur powder (HS), and controlled chemicals (ST), while non treatment (NT) was used as control. After the early part of disease outbreak, the plants were treated four times with 7 days interval. The incidence of leaf spot, efficacy, phytotoxicity and control value were investigated. Based on the efficacy test, incidence of leaf spot is lowest at 15.6% in plant extracts (BP). Other biopesticides showed higher incidence - 16.7% for microbial agent (DM), 17.8% for microbial agent (PJ), 18.9% for sulfur powder (HS) and 71.1% for non treatment (NT), compared with 11.1% in controlled chemicals (ST). The microbial agent (PJ), sulfur powder (HS), plant extracts (BP) and microbial agent (DM) were not damaged in the phytotoxicity test. In the test of application dose, time and methods, the control value was highest in plant extracts (BP) at 78.1%, followed by microbial agent (DM) at 76.6%, microbial agent (PJ) at 75.0%, and sulfur powder (HS) at 73.4% , compared with controlled chemicals (ST) at 84.4%. Conclusion : It is possible to use biopesticides at the proper time to prevent leaf spot in Adenophora triphylla var. japonica Hara.

Background : Adenophora triphylla var. japonica Hara. is used to cure respiratory/ lung diseases, which makes it an important oriental medicinal herb. It is one of the five Sam (ginseng), which includes Panax ginseng, Salvia miltiorrhiza, Sophora flavescens, and Scrophularia buergeriana. In 2015, Korea imported 24 tons of medicinal herbs worth US$95,000. This study was carried out to investigate the effect of several mulching materials on the growth and root yield of A. triphylla var. japonica using organic fertilizer. Methods and Result : Seeds of A. triphylla var. japonica were collected at the nursery of the Department of Herbal Crop Research, NIHHS, RDA in 2015. Mulching materials used are black plastic, black woven fabric, and biodegradable plastic. Hand weeding was done to serve as control. The soil was fertilized two months before planting. Seeds of A. triphylla var. japonica were sowed in tray in early March and were allowed to grow for two months. It was planted in early May with planting density of 30 ㎝ x 15 ㎝ and was covered with various mulching materials. Parameters investigated are survival rate, aerial part and root growth characteristics, and yield at harvest time. Results showed that biodegradable plastic is most favorable for plant growth with height of 17.2cm, leaf length of 13.4cm and leaf width of 5.6 ㎝. Dry plant weight was heaviest in biodegradable plastic treatment at 2.8 g, and lightest at 2.5 g when planted without mulch. Biodegradable plastic has the highest dry root weight of 19.3 g, followed by black vinyl (18.4 g), woven fabric (18.3 g) and without mulch (17.9 g). The yield obtained per 10 a was highest in biodegradable plastic treatment at 174 ㎏, while black vinyl and woven fabric treatments produced 169 ㎏ and 160 ㎏, respectively. Plants without mulch had the lowest yield of 157 ㎏. Conclusion : In the above results, biodegradable plastic and woven fabric are considered suitable mulching materials for the cultivation of A. triphylla var. japonica.

Background : There are about 30 species of Adenophora triphylla which grow wild in Korea. but they are very similar so that it is difficult to distinguish with the naked eye. Therefore, it is separated by conformational differences such as leaf shape and flower size. However, these morphological distinction is not useful because even in the same type there are differences by a growth phase or the environment. It is difficult to identify and classify Adenophora triphylla because the phenotypes between plant individuals are diverse greatly even within the same type. For this reason, we will investigate the flowering characteristics of Adenophora triphylla and this information will be used as the basis for the breeding line. Methods and Results : We used the second year Adenophora triphylla in Herbal Crop Research Division, Department of Herbal Crop Research in Eumseong. We Investigated flowering characteristics from June 2015 to November 2015. The 34 individuals of 134 individuals were died by pests and diseases. Depending on the flowering and fruition characteristics were classified into 5 populations, except for the early and late maturing individuals. Conclusions : The flowering period of Adenophora triphylla is 1 month from mid-July to mid-August, and it takes 15 days from the flowering season to the 70% of flowering. It usually takes about 60 days from flowering to fruition, and the fruition is finished in mid-October.

Background : Angelica gigas N., one of the herbs has been used most frequently with Glycyrrhiza uralensis. It is usually cultivated in semi-alpine of more than 400m in North Central province of South Korea. However its production is becoming more unstable due to the climate change especially abnormally high temperature. In particular, the environmental change may cause not only a change in production but also a change in active ingredient of Angelica gigas N. This study was performed to investigate these problems. Methods and Results : This study was carried out in temperature gradient tunnel(TGT) in Pyeongchang, Gangwon province. Temperature conditions were maintained the ambient+0~2℃ (T1), ambient+2~4℃(T2), ambient+4~5℃(T3) throughout the year. We planted and grown one year old seedlings of Angelica gigas N. from mid-April to early November. As a result, there was no difference in early growth of Angelica gigas N. among the temperature range but there was a difference between each treatment region from the survey in June. Average length of soot in T1, T2, T3 was the largest(36cm), medium(27cm), smallest(19cm) respectively. This trend was similar to a survey in September and November. Each of death rate was 0%(T1), 19%(T2), 71%(T3). There was no bolting according to the temperature. The yield of a plant of Angelica gigas N. was 476g(T1), 76g(T2), 26g(T3), respectively. Production of Angelica gigas N. is foreseen 84.7% and 94.5% reduction if the temperature rises to 3±1℃ and 4±1℃ more than it is at present based on temperature in Pyeongchang 2015. Total amount of Decursin was reduced at higher temperatures. Conclusion : The results of this study show that quality and production of Angelica gigas N. is expected to decline significantly if the temperature rise than the current. Therefore development of various techniques is required to response to climate change.

Background : The roots of Curcuma longa Radix is used to make herbal medicine, which is registrated in the Korean Pharmacopoeia. This species is a perennial herb, and is distributed throughout tropical and/or sub tropical regions. High temperature and humidity with small difference between daytime and nighttime is the finest environment condition in this species. Recently, demand for C. longa has been expanded to cultivation area as central region from southern region. Therefore, this study aimed to investigate the effect of harvest time on the growth, yield characteristics and main composition contents in C. longa. Methods and Results : Curcuma longa rhizomes were harvested from farmland in Jindo, Jeollanam-do in end October, early November, middle November, end November, early December, middle December and end December 2015. Harvested rhizome were dried in hot air drier, and it was investigated as growth characteristics and yield. Contents of curcumin, demethoxycurcumin, bisdemethoxycurcumin was analyzed from dried powder of rhizome. The analysis column was used to Phenomenex, Kinetex 2.6um F5 100A 100*2.1mm. 10 mg of standard was taken, 80% MeOH was added to dissolve in 10 ㎖ flask. 0.5, 1, 2, 4 ㎖ was taken from dissolved solution and then was added to 10 ㎖ of 80% MeOH. It was filtered through 0.45 ㎛ filter and then 5 μℓ was taken to make standard solution. Analytical calibration curve was measured to diluted solution within sample concentration. 2 g of ground sample was taken, 40㎖ of 80% MeOH was added to extract by ultrasound sonication during 60 minutes and then it was filtered through 0.2 ㎛ filter to analyze component content. Conclusion : The root weight significantly increased from 376.1 g in end October to 695.9 g in end December as 1.8 double. Curcumin contents differed significantly among harvest times in end November and early December. Demethoxycurcumin increased from early November to end November, and Bisdemethoxycurcumin increased to the early November.

Background : Adenophora triphylla var. japonica Hara. is used to cure internal organ, which is called to important one of the five “Sam (ginseng)” oriental medicinal herbs included Panax ginseng, Salvia miltiorrhiza, Sophora flavescens, Scrophularia buergeriana. Korea imported 24 ton 129 thousand dollar for medicinal herbs and 23 ton 29 thousand dollar for food at 2013. This study was carried out to investigate the effect of several organic compost on the growth and root yield of A. triphylla var japonica with organic fertilizer. Methods and Results : Seeds were collected from Adenophora triphylla var. japonica Hara. at last year in nursery of Department of Herbal Crop Research, NIHHS, RDA. Four types organic fertilizer (germiculture (GG), granular mix oil cake (CJ), mixed organic (DJ), plant oil cake (TG)), chemical fertilizer (CF) and non-fertilizer (NF) were treated. It was fertilized on soil before 2 month of planting. Seeds A. triphylla var. japonica were sowed in tray at early March and then were grown during 2 month. It were planted in furrowed nursery at early with 30 × 10 cm planting distance and black vinyl covering condition. Soil chemical property, survival rate, aerial part and root growth characteristics and yield were investigated at harvest time. For main composition analysis, 10 mg of standard (b-sitosterol, lupenone) was taken, 80% MeOH was added to dissolve in 10 ㎖ flask. 0.5, 1, 2, 4 ㎖ was taken from dissolved solution and then was added to 10 ㎖ of 80% MeOH. It was filtered through 0.45 ㎛ filter and then 5 μℓ was taken to make standard solution. Analytical calibration curve was measured to diluted solution within sample concentration. 2 g of ground sample was taken, 40㎖ of 80% MeOH was added to extract by ultrasound sonication during 60 minutes and then it was filtered through 0.2 ㎛ filter to analyze component content. Conclusion : GG and DJ were the most suitable for organic cultivation of denophora triphylla var. japonica. Content of beta-sitosterol showed the highest value in DJ treatment and the lowest value in CF. Content of lupenone showed the highest value in DJ and the lowest value in CJ.

Background: This study aimed to investigate the effect of harvest time on the growth, yield characteristics and loganin content in Dipsacus asperoides Wall. Methods and Results: Dipsacus asperoides seedlings were planted within a nursery environment in early May 2015 and harvested in early, middle and late October 2015, and early November 2015. Harvest time did not result significant differences in the plant height, stem diameter, branch length, leaf width and aboveground dry weight moreover, no significant differences were observed in root length, number of roots and root diameter. However, the diameter of lateral roots was greater in the harvests from the late October and period thereafter. The highest values of root dry weight and yield were recorded in early November. Specifically, the yield significantly increased from 205 ㎏/10 a (index: 100) in early October to 358 ㎏/10 a (index: 175) in early November, in terms of root part weight. Loganin contents of D. asperoides differed significantly among harvest times raging from 0.0766% in early October to 0.1704% in late November, thereby showing an increasing trend in later harvest times. Conclusions: These results suggest that the optimum harvest time for D. asperoides is early November, when the yield is the highest. Harvest time significantly affected loganin contents, which constantly increased from early October until early November.

Background : The aim of the present study was to identify an effective physicochemical control method to reduce Fusarium species infestation in adlay (Coix lacryma-jobi L.) before and after harvesting. Methods and Results : We observed that prochloraz emusifiable concentrate and hexaconazol prochloraz emusifiable concentrate strongly inhibited the mycelial growth of 10 Fusarium species. Strong growth inhibitions and cell lysis were observed following treatment with 4% NaOCl solution. The total number of fungi detected were lower follwing treatment with thiophanatemethyl triflumizole wettable powder (1.1 × 104 CFU/g), hexaconazol prochloraz emulsifiable concentrate (1.2 × 104 CFU/g), carboxin thiram dustable powder (1.6 × 104 CFU/g) and prochloraz emulsifiable concentrate (1.7 × 104 CFU/g) than in the non-treated control (7.7 × 104 CFU/g). The reduction of Fusarium fungi varies with the concentration and soaking time of NaOCl solution. Fungal detection was not observed after soaking in NaOCl solution for 24 h and harmful effects were not observed for plant growth by NaOCl after soacking for 6 - 12 h. Conclusion : Soaking seed for 6 - 12 h in 4% NaOCl could be an effective method of disinfectant treatment for the control of Fusarium fungi in adlay seeds.