The importance of the immune system in the pathogenesis of periodontitis is being emphasized. To investigate the effects of different immune statuses on the progression of periodontitis, we induced periodontitis in neutrophil-deficient, lymphocytedeficient, and dual neutrophil- and lymphocyte-deficient mice. The groups included BALB/c mice (Con-lym+/neu+), periodontitisinduced BALB/c and SCID mice (Peri-lym+/neu+ and Peri-lym–/neu+), and periodontitis-induced BALB/c and SCID mice treated with anti-Ly6G antibody (Peri-lym+/neu– and Peri-lym–/neu–). Periodontitis was induced by ligation of the mandibular first molar. Mice were sacrificed on days 0 (D0), 3 (D3), and 7 (D7) after ligation, and blood and periodontal tissue were analyzed. On D3, the AB area in the Peri-lym–/neu+ group was larger than in the Peri-lym+/neu+ group. The osteoclast on D3 was lower in the Peri-lym+/neu– group compared to the Peri-lym+/neu+ group. On D7, Ly6G+ cells were lower in both blood and periodontal tissue in the Peri-lym+/neu– group compared to the Peri-lym+/neu+ group, whereas CD3+ cell counts were higher only in periodontal tissue. In the Peri-lym–/neu– group, Ly6G+ cells were lower in both blood and periodontal tissue than in the Peri-lym–/ neu+ group, whereas CD3+ cells were higher in blood only. These results suggest that lymphocyte depletion delays AB loss and neutrophil depletion influences osteoclast formation at the early stage of periodontitis. In the late stage of periodontitis, lymphocyte depletion does not increase lymphocyte infiltration into periodontal tissue, even with neutrophil depletion. Collectively, different immune states may influence distinct stages of periodontitis progression.

The role of the gut microbiota in colorectal cancer (CRC) development has garnered attention, highlighting probiotics as potential adjuncts in CRC prevention and treatment. In recent years, probiotics and their derivatives have demonstrated mechanisms that may contribute to anticancer properties. This study investigates the cytotoxic effects of Bifidobacterium bifidum KCTC 3357, Lacticaseibacillus rhamnosus KCTC 5033, Limosilactobacillus reuteri VA 103, Bacillus galactosidilyticus VA 107, and Lactococcus taiwanensis VE101 on CT-26 mouse colon carcinoma cells using live cells, heat-killed cells (paraprobiotics), and cell-free supernatants (CFS, postbiotics) through an MTT assay. The results indicate that live bacterial strains, such as KCTC 3357, VA 103, and VA 107, promoted CT-26 cell viability, while heat-killed cells and CFS exhibited dose-dependent cytotoxicity. Inactivated forms of KCTC 3357 and VE 101, as well as CFS at 10 mg/mL concentration of KCTC 5033, VA 103, and VE 101, showed the strongest antiproliferative effects. These findings suggest that non-viable probiotic derivatives, such as paraprobiotics and postbiotics, offer promising therapeutic potential for CRC, providing a safer and more stable alternative to live probiotics. However, further research is required to explore their mechanisms of action, in vivo efficacy, and potential clinical applications.

Osteoporosis, characterized by excessive osteoclast activity and disrupted bone remodeling, remains a significant health concern. This study investigated the potential of shiitake mushroom (Lentinula edodes) extracts, prepared with various drying methods, to inhibit osteoclast differentiation and promote bone formation. Mushroom stipe extracts, irrespective of the drying method, effectively suppressed osteoclast differentiation by downregulating nuclear factor of activated T-cells, cytoplasmic 1 expression in osteoclast precursor cells treated with macrophage colony-stimulating factor and receptor activator of nuclear factor kappa-Β ligand (RANKL), whereas the mushroom cap extracts exhibited minimal inhibitory effects. This disparity highlights the importance of part-specific analyses as the stipes contained higher levels of bioactive molecules, including denatonium, which was identified as a key RANKL signaling disrupter. In contrast, the caps lacked the anti-osteoclastogenic activity observed in the stipes, which were notably consistent across the freeze-drying, air-drying, and hot-air drying methods, indicating the stability of the bioactive compounds under various processing conditions. In vivo studies using an osteoporosis-induced zebrafish model further validated the therapeutic potential of the stipe extracts, which restored bone mineralization and mitigated prednisolone-induced bone loss. These findings emphasize the importance of identifying and optimizing specific mushroom parts for their unique therapeutic properties. Shiitake mushroom stipe extracts are a natural product potentially useful in osteoporosis therapy. This therapy would be a promising alternative to conventional treatments, such as bisphosphonates, which are associated with adverse side effects.

Probiotics have been evaluated as therapeutic agents for cancer treatment in an increasing number of studies. This study investigated the inhibitory and cytotoxic effects of specific Lactobacillus strains on a human colorectal adenocarcinoma cell line (HT-29). The strains assessed were Limosilactobacillus (L.) reuteri VA 102, Ligilactobacillus (L.) animalis VA 105, and Limosilactobacillus (L.) reuteri KCTC 3594 (ATCC 23272). The viability of HT-29 cells was evaluated using the MTT assay. The findings revealed that cell-free supernatants (CFS) exhibited significant anticancer effects. Heat-inactivated L. reuteri VA 105 and L. reuteri KCTC 3594 induced a pronounced reduction in cell viability. Furthermore, live cultures of L. reuteri VA 105 and L. reuteri VA 102 also showed reduced cell viability compared to the control group. These results suggest that CFS and heat-inactivated cells may be more suitable for therapeutic applications than live bacteria owing to their improved safety profiles and reduced potential for adverse effects. Our findings also emphasize the potential anticancer benefits of these LAB strains.

Drought is one of the environmental factors inhibiting plant productivity and growth, leading to oxidative damage. This study aims to identify the role of sodium hydrosulfide (NaHS) as a hydrogen sulfide (H2S) donor in drought stress tolerance in Brassica napus. Drought-induced stress symptoms appeared eight days after treatment, showing wilted leaves and a significant reduction of leaf water potential. Drought-induced increase of lipid peroxidation was significantly reduced by NaHS application. NaHS-treated plants mitigated stress symptoms under drought conditions by reducing hydrogen peroxide (H2O2) content, confirmed with H2O2 localization in situ. Furthermore, NaHS promotes photosynthetic activity by maintaining chlorophyll and carotenoid content, thereby supporting plant growth under drought conditions. Pyrroline-5-carboxylate and proline contents were significantly increased by drought but further enhanced by NaHS treatment, indicating the important roles of proline accumulation in drought stress tolerance. In conclusion, this study provides valuable insight into the roles of NaHS in alleviating drought stress by reducing oxidative stress and promoting proline accumulation. Therefore, NaHS may serve as an effective strategy to enhance crop production under drought-stress conditions.

Probiotic lactic acid bacteria are live microorganisms that provide health benefits when administered in adequate amounts and may exhibit antiproliferative effects on various cancer cell lines, including colon cancer. This study investigates the cytotoxic effects of three Lactobacillus strains - Limosilactobacillus (L.) reuteri VA 102, Ligilactobacillus (L.) animalis VA 105, and Limosilactobacillus (L.) reuteri KCTC 3594 (ATCC 23272) - on mouse colon carcinoma cells (CT-26). Live cells, heat-killed cells, and cell-free supernatant (CFS) of Lactobacillus sp. were prepared and used to treat CT-26 cells at different concentrations. The cytotoxic effect was assessed using the MTT assay. The results indicated that the CFS of all strains significantly reduced the viability of CT-26 cells in a dose-dependent manner, with the VA 102 strain showing the most pronounced effect. Heat-killed cells of L. reuteri VA 102 and L. reuteri KCTC 3594 (ATCC 23272) also reduced cell viability. These findings suggest the potential anticancer properties of these Lactobacillus strains and indicate that CFS and heat-killed cells may offer a safer and more effective alternative to live bacteria for therapeutic applications. Our study contributes to the understanding of the potential of Lactobacillus strains, particularly L. reuteri VA 102, L. reuteri KCTC 3594 (ATCC 23272), and L. animalis VA 105, as possible candidates for cancer treatment and control.

This study explores the development of English textbooks in North Korea through corpus-based analysis aimed at illuminating the differences between materials produced during the Kim Jong-il and Kim Jong-un regimes. In the context of educational reforms and changing political ideology, this study investigates BNC/COCA-based lexical coverage and the key lexical features of North Korean middle school English textbooks, highlighting the complexity, vocabulary, and readability of the learning materials. The findings revealed that the Kim Jong-un regime had implemented reforms to improve English language education, with increased lexical diversity, textual complexity, and vocabulary exposure. Although no significant differences were found between the two regimes regarding the lexical coverage of textbooks, the Kim Jong-un regime’s textbooks exhibited improvements in diversity, readability, and complexity. This study contributes to a broader understanding of the interplay between political ideology and English language education in North Korea, offering insights that have implications beyond the North Korean context and encouraging reflection on the nation-driven educational reform.

Climate change has led to increased insect pests and pest distribution changes. Traditionally, chemical control using synthetic pesticides has been the main method for pest management, but the emergence of pesticide-resistant pests has become a problem. There is a need to develop new pest control agents to overcome these issues. Entomopathogenic fungi used in pest management have minimal environmental side effects and possess a mechanism of action distinct from that of synthetic pesticides. However, there is a need for the development of technologies to maximize the insecticidal effects of fungi against pests, and expressing and releasing dsRNA within the fungi can preemptively knock out the activation of the insect’s defense system, thereby enhancing the insecticidal effect. Controlling insect defense genes and using entomopathogenic fungi as bio-carriers forms a new pest management strategy. This approach, described as a “microbial insecticide agents development strategy of cassette concept, ” can versatilely modify genes and microbes. It is expected to overcome the limitations of synthetic pesticides.

Synthesis of extremely competent materials is of great interest in addressing the energy storage concerns. Manganese oxide nanowires ( MnO2 NWs) are prepared in situ with multiwall carbon nanotubes (MWCNT) and graphene oxide (GO) using a simple and effective hydrothermal method. Powder XRD, Raman and XPS analysis are utilized to examine the structural characteristics and chemical state of composites. The initial specific discharge capacity of pure MnO2 NWs, MnO2 NWs/ MWCNT and MnO2 NWs/rGO composites are 1225, 1589 and 1685 mAh/g, respectively. The MnO2 NWs/MWCNT and MnO2 NWs/rGO composites showed stable behavior with a specific capacity of 957 and 1108 mAh/g, respectively, after 60 cycles. Moreover, MnO2 NWs/rGO composite sustained a specific capacity of 784 mAh/g, even after 250 cycles at a current density of 1 A/g showing outstanding cycling stability.

강원특별자치도 18개 시군을 대상으로 감나무와 콩에 발생하는 미국선녀벌레, 썩덩나무노린재, 담배거세미 나방의 발생을 조사하였다. 돌발해충인 미국선녀벌레는 약충기인 6월과 성충기인 10월에 발생조사를 진행하고 남방계해충인 담배거세미나방과 썩덩나무노린재는 7월과 9월 콩 재배기에 발생조사를 진행하였다. 미국선녀 벌레는 강원지역에 있는 감나무에서 발생을 확인할 수 없었고. 감나무 주변 기타 수목에서 가지당 평균 6.7마리 발생하였다. 담배거세미나방은 7월, 속초를 제외한 모든 시군에서 발생이 되었으며, 춘천, 횡성에서 트랩당 60마 리가 넘는 발생밀도를 보였으며, 원주, 강릉, 화천 등 에서는 트랩당 1마리로 낮은 발생밀도를 나타냈다. 썩덩나무 노린재는 7월 춘천, 속초, 홍천 등 7개 시군을 제외한 11개 시군에서 트랩에 포획되는 것을 확인하였으며, 화천에 서 트랩당 26마리로 최고발생밀도를 보였다.