The carbon anode material for lithium-ion battery was prepared by pyrolysis fuel oil and waste polyethylene terephthalate (PET) additive. The pitch was synthesized as a medium material for carbon anode by heat treatment. The waste PET additive improved the softening point and thermal stability of the pitch. La and Lc of the anode material (heat-treated pitch) increased at higher treatment temperature but decreased by waste PET additive. The electric capacity was evaluated based on effects of defective cavity and developed graphite interlayer, respectively. When the La and Lc of the anode material decreased, the electric capacity by cavity increased based on defective graphite structure. Therefore, the addition of waste PET causes the improved capacity by the cavity. The anode material which has a high efficiency (over 95%) and C-rate (95%, 2 C/0.1 C) was obtained by controlling the process of heat treatment and PET addition. The mechanism of lithium-ion insertion was discussed based on effects of defective cavity and developed graphite interlayer.

Supply chain management can be defined as an information system that connects the inside and outside of a company. Its purpose is to systematically and strategically manage the flow of information, resources and services to improve the long-term performance of the entire organization, including individual companies connected to the supply chain, and the quality of service provided to customers. The ultimate goal of SCM is to create synergy through organic integration of supply and demand based on cooperation and collaboration with stakeholders in the supply chain. This study is based on the hypothesis that the company's management performance will improve as the level of SCM improves. Most of the previous studies dealt with the relationship between corporate performance and SCM in the IT area. In this study, research was conducted through human capacity with IT system. The causal relationship was demonstrated, and there was a difference in the perception of the results of this study depending on whether or not smart factories were consulted in the era of the 4th Industrial Revolution. There is a need to examine the links between management's value chain and its causal relationship.

This study investigated the efficacy of four Brucella (B.) abortus recombinant proteins, namely adenylate kinase (Adk), nucleoside diphosphate kinase (Ndk), 50S ribosomal protein (L7/L12) and preprotein translocase subunit (SecB), as a combined subunit vaccine (CSV) against B. abortus infection in BALB/c mice. Immunoblotting assay showed that these four recombinant proteins as well as pcold-TF vector reacted individually with Brucella-positive serum, but not with Brucella-negative serum. The peripheral blood CD4+ T cell population was increased in CSV-immunized mice compared to PBS and pcold-TF vector groups. In addition, CSV and pcold-TF groups displayed induced IgG1 and IgG2a antibodies production compared to PBS and RB51 group, whereas IgG2a titer was higher than IgG1 titer in CSV group. The secretion profiles of IgG1 and IgG2a production together with an enhancement of CD4+ T cell population suggested that CSV did not only induce T helper 1 (Th1) T cell immunity but also humoral immunity. Therein, Th1 T cell immunity is more predominant in eliminating intracellular bacteria B. abortus. Furthermore, CSV immunization significantly reduced the bacterial burden in the spleen as well as the spleen weight in comparison to PBS and pcold-TF groups. Altogether, combination of these antigens could be potential to induce protective immunity against B. abortus infection in animals.

A new discrete bis-dithiolene complex, [ PPh4]2[Zn(DMED)2] (1; DMED = 1,2-dicarbomethoxy-1,2-dithiolate) with sulfurbased radical character was synthesized and structurally characterized. Complex 1 is stable and exhibits a square planar geometry around the zinc metal. 1 forms nanospheres through a one-pot water-induced self-assembly in a mixture of solvents (acetonitrile–water). These nanospheres were further decorated with water-soluble carbon nanotubes (wsCNTs) through hydrogen bonding between the peripheral –COOCH3 groups of 1 and surfacial carboxyl groups of wsCNTs to assemble into a spherical nanocomposite. The as-prepared nanocomposite showed fluorescence emissions in visible region due to the separation of energy states of the nanospheres assisted by wsCNTs, suggesting the future possibilities of these new materials for use in biomedical application.

목적 : 이 설문 연구는 의료민영화와 안경원의 법인화 문제에 대한 일반 소비자의 인식도를 조사하고 그 결과를 안경사 집단에 대한 선행연구와 비교·분석하는 것을 목적으로 하였다. 방법 : 2018년 5월부터 6월까지 서울·경기 지역에서 150명의 일반 소비자를 대상으로 의료민영화와 안경원의 법인화 문제에 대하여 대면설문조사를 실시하고 응답 결과를 통계분석 하였다. 결과 : 일반 소비자 집단은 의료민영화 문제에 대하여 일관되게 부정적인 인식을 나타내었으나, 안경원의 법인화 문제에 대해서는 부정적인 인식이 많지 않고 중립적인 응답의 비율이 매우 높았다. 안경사 집단에 대한 유사한 설문 조사 결과와 비교했을 때, 의료민영화에 대한 인식은 두 집단이 비슷했으나 안경원 법인화에 대한 인식은 큰 차이를 보였다. 결론 : 반 소비자 집단의 안경원 법인화 문제에 대한 부정적인 인식도가 높지 않으므로, 안경사 집단은 일반 소비자 집단을 대상으로 안경원 법인화 문제에 대한 적극적인 홍보를 강화할 필요가 있다.

BALB/c mice were vaccinated with Brucella (B.) abortus recombinant protein L27 (50S ribosomal protein L27) cloned into a pMal vector system. L27 was induced, purified and injected intraperitoneally (IP). Mice were vaccinated on 0-, 15- and 35-day. Serum cytokines were evaluated on 36- and 49-day from first vaccination. Mice were intraperitoneally infected with 5×104 CFU of virulent B. abortus 544 on day-50 and sacrificed after two weeks from infection. Bacterial burden from the spleen was quantified and showed a 0.7- and 0.9-log reduction in vaccinated mice in comparison to PBS and MBP (maltose binding protein) groups respectively. Cytokines in the serum demonstrated increased interferon-gamma (IFN-γ) and other pro-inflammatory cytokines such as macrophage chemoattractant protein-1 (MCP-1) and interleukin 6 (IL-6). On the other hand, interleukin 10 (IL-10) was attenuated in the sera of vaccinated mice. This cytokine profile is indicative of a cell-mediated type of immune response which is favorable for the eradication of intracellular infections. The current study showed the potential of another B. abortus ribosomal protein in inducing protective immunity against B. abortus infection.

전 세계적으로 생물다양성 감소가 가속화 되고 있으며, 특히 외래생물의 유입은 생물다양성을 위협하는 가장 심각한 요인 중 하나이다. 우리나라 또한 외래생물의 유입이 증가하고 있는데, 환경부 기준 외래생물 은 2011년 1,109종에서 2013년 2,167종으로 두 배 가까이 증가되었다. 2,167종 중 곤충류는 136종으로 많은 외래곤충의 유입이 확인되었고, 이외에 공식적으로 집계되지 않은 종을 포함하면 그 수는 훨씬 늘어날 것이다. 2014년부터 국립생태원의 외래생물 안전관리 연구는 위해우려종 발굴, 외래생물 전국 서식실태조사, 외래생물 정밀조사, 생태계 위해성 평가, 생태계교란 생물 모니터링 등 크게 5가지로 구분된다. 위해우려종 발굴 및 지정건의를 통해 유입 전 단계부터 생태계교란 생물의 지정 건의 및 모니터링을 통해 유입 후 관리까지 5가지의 과제가 톱니바퀴처럼 물려가며 수행되고 있다. 국립생태원 외래곤충 분야의 주요 연구 성과는 260여종의 위해우려종 후보종 발굴, 5종의 외래생물 정밀조사, 8종의 생태계 위해성평가를 수행하였다. 그 중 4종을 생태계교란 생물로 지정 건의하여 1종(붉은불개미)이 생태계교란 생물로 지정되었다. 또한, 꽃매미를 포함한 2종의 생태계교란 생물을 모니터링 하고 있으며, 국내 유입될 경우 생물다양성에 미치는 영향을 널리 알리고자 ’위해우려 외래곤충 100종’ 책자를 발간하여 배포하기도 하였다.

In this study, we examined the protective immunity of a combination of seven Brucella abortus recombinant proteins; superoxide dismutase (rSodC), riboflavin synthase subunit beta (rRibH), 50S ribosomal protein (50s rL7/L12), nucleoside diphosphate kinase (rNdk), malate dehydrogenase (rMDH), arginase (rRocF), and elongation factor (rTsf) cloned in a pMal vector system and expressed in DH5α. Mice groups were immunized thrice with a combined subunit vaccine (CSV-7) at 0, 2, and 5 weeks and subsequently challenged with B. abortus at 5 × 104 CFU at 6 weeks. At four weeks post-infection, the mice were sacrificed and the bacterial burden in their spleens was quantified. Results revealed bacterial log reductions of 0.63 and 0.34 in comparison to PBS and maltose-binding protein (MBP), respectively. Cytokine profiling revealed a marked increase in IFN-γ (interferon-gamma), MCP-1 (macrophage chemoattractant protein-1) and IL-6 (interleukin 6) cytokines at 5-weeks post-immunization. On the other hand, only TNF was heightened at 7-weeks post-immunization. In general, this cytokine profile is consistently reflective of a Th1 immune response, which is beneficial for host immunoresistance.

Cryopreservation is mainly used for preservation of boar sperm. However, this method stresses the sperm by reactive oxygen species (ROS), and the conception rate and the litter size are not more efficient than the liquid preservation of spermatozoa. Therefore, we use chitosan which is a natural product derived antioxidant compound. We used GnHA (chitosan+hyaluronic acid) and GnHG (chitosan hydrogel) as chitosan complexes to cryopreserve boar sperm for improve sperm metabolism and function. Sperm parameter (sperm motility, progressive motility, path velocity, straight-line velocity, curvilinear velocity) is measured by computer-assisted sperm analysis (CASA) using frozen sperm with GnHA or GnHG (0, 0.25, 0.5, 1 mg/mL), respectively. Also, lipid peroxidation analysis using malondialdehyde (MDA) is performed to confirm the antioxidative effect of chitosan in frozen spermatozoa. CASA analysis showed GnHA and GnHG are effective against cryopreserved boar sperm. And antioxidant effect is measured by lipid peroxidation analysis. GnHA and GnHG, which is chitosan complex are effective for boar sperm cryopreservation by antioxidant effect.

This study evaluated the protective effects of a combination of eight B. abortus recombinant proteins that were cloned and expressed into a pMal vector system and DH5α: nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12), malate dehydrogenase (rMDH), DNA starvation/stationary phase protection protein (rDps), elongation factor (rTsf), arginase (rRocF), superoxide dismutase (rSodC), and riboflavin synthase subunit beta (rRibH). The proteins were induced, purified, and administered intraperitoneally into BALB/c mice. The mice were immunized three times at weeks 0, 2, and 5 and then infected intraperitoneally (IP) with 5×104 CFU of virulent B. abortus 544 one week after the last immunization. The spleens were collected and the bacterial burden was evaluated at four weeks post-infection. The results showed that this combination produced a significant reduction of the bacterial burden in the spleen with a log reduction of 1.01 compared to the PBS group. Cytokine analysis revealed induction of the cell-mediated immune response in that TNF (tumor necrosis factor) and proinflammatory cytokines IL-6 (Interleukin 6) and MCP-1 (macrophage chemoattractant protein-1) were elevated significantly. In summary, vaccination with a combination of eight different proteins induced a significant protective effect indicative of a cell mediated immune response.