본 연구는 한반도 유사 사례 중 하나인 1976년 판문점 도끼 사건에 대 한 한미일 3국의 관점을 역사적인 측면에서 관련 문서를 통해 고찰하였 다. 한국 정부와 국회는 북한과 공산주의에 관한 강렬한 적대감을 나타내 었다. 미국 백악관도 북한의 전적인 책임을 강조하며 북한을 비난하였다. 미국 의회는 판문점 도끼 사건을 규탄하였지만, 동시에 한국의 국내 인권 문제에 대해서도 논하였다. 이에 박정희 정권은 미국 의회의 대응이 매우 모호하다고 판단하여 불편함을 나타내며 미국 국무부에 도움을 요청하였 다. 한편, 일본 정부와 자민당은 판문점 도끼 사건에 대한 미국의 초기 대응에 공감하였다. 그러나 일본 국회에서 다른 정당은 한반도 유사시 미 국의 동맹인 일본이 개입하게 될 가능성을 매우 우려하였다. 본 연구는 한반도 유사 사례 중 하나인 1976년 판문점 도끼 사건이 발생하였던 당 시 한미일 3국의 시각이 모두 협력적이지는 않았음을 강조한다.

본 연구는 담액수경재배(Deep Flow Technique, DFT)로 재배된 잎들깨(Perilla frutescens)의 생장 및 생리 적 반응에 미치는 인산결핍의 영향을 분석하였다. 인산 결핍 처리는 야마자키 표준 배양액을 수정하여 0% (대조 구), 30%, 50%, 70%, 100%의 5 수준으로 달리 조성하였다. 처리 20일부터 80일까지 인산 결핍 수준이 증가함에 따라 초장, 엽수, 마디 수, 경직경이 유의미하게 감소하였다. 80일째에는 100% 인산 결핍 처리에서 초장이 10.1cm, 엽수는 식물체당 2.7장으로 급감하였으며, 이는 대조구의 73.5cm 및 12.8장에 비해 현저한 생장 저해를 나타냈다. 또한, 인산 결핍 조건에서 생체중과 건물중 모두 현저히 감소하였으며, 대조구의 생체중은 93.4g 이었으나 100% 인산결핍 처리구에서는 2.1g으로, 건물중은 11.8g에서 0.4g으로 크게 감소하였다. 인산 결핍 정도가 증가할수록 하 부엽에서 황화(Chlorosis) 및 괴사(Necrosis)와 같은 생리장해 증상이 뚜렷하게 나타났다. 이러한 결과는 인산이 잎 들깨의 영양생장과 건물생산량 증가에 필수적인 역할을 수행함을 나타내었다.

본 논문은 한반도 유사 사례 중 하나인 KAL 858 사건 당시 한미일의 대응을 검토하였다. 본 연구는 KAL 858 사건이 발생했던 전두환 정권 시기와 노태우가 대통령으로 취임했던 1988년 2월 25일 이후 시기를 구 분하여 한국, 미국, 일본에서 생산된 1차 자료들을 중심으로 검토하였다. KAL 858 사건 발생 당시 한국, 미국, 일본은 공통적인 반응을 나타냈 다. 우선 KAL 858 사건이 일어났던 1987년 11월부터 한국, 미국, 일본 모두 협력하여 북한을 규탄하며 제재를 가하였다. 반면, 노태우 대통령 취임 이후 한국, 미국, 일본은 북한을 규탄하면서도 군사적인 보복 등의 강력한 제재와 관련된 고려를 줄였다. 본 연구는 1987년 KAL 858 사건 에서 한국, 미국, 일본이 협력하는 모습을 보였음을 제시한다.

Gentamicin is an aminoglycoside antibiotic effective against aerobic gram-negative bacteria and is also used in veterinary medicine, particularly in the swine and bovine industries. However, no gentamicin product is currently approved for treating equine diseases in Korea. The present study aims to examine the time-dependent residue of gentamicin in horses after intravenous injection (IV) via jugular vein. The test product was injected at 6.6 mg/kg BW via jugular vein in nine horses. Blood was collected from the horse's jugular vein at 15 minutes, 30 minutes, 1, 4, 8, 12, 24 and 48 hours after injection. To purify the gentamicin in serum, 100μL of 20 mM HFBA in DW, 100 μL of 30% trichloroacetic acid and 300 μL of 20 mM heptafluorobutyric acid (HFBA) in acetonitrile (ACN) were added to 500 μL of serum and supernatant was applied to LC-MS/MS after centrifugation. LC-MS/MS-8050 analyzed the level of gentamicin in serum with Electrospray ionization (ESI) and multiple reaction monitoring (MRM) positive mode. Gentamicin C1 was 478 m/z and product ions were 322, 157 m/z. Precursor ion of Gentamicin C1a was 450 m/z and product ions were 322, 160 m/z. Precursor ion of Gentamicin C2 and C2a was 464 m/z and product ions were 322, 160 m/z. The LC column was a C18 and mobile phase composed of 20 mM HFBA in 5% ACN and 20 mM HFBA in 50% ACN. The amount of gentamicin was calculated by adding four components of gentamicin (C1, C1a, C2 and C2a). The pharmacokinetic parameters of gentamicin were calculated by the WinNonlin program. The Cmax of gentamicin in horse serum was 93 ± 17 μg/kg and the Tmax was 0.25 ± 0 hours. The T1/2 was 6.41 ± 2.32 hours and the CLt was 0.05 ± 0.01L/hr/kg. The Vd was shown as 0.44 ± 0.13 L/kg and the MRT was 1.98 ± 0.55 hours. In conclusion, our data provides useful pharmacokinetic parameters for gentamicin in horses following IV injection.

본 연구에서는 국내에서 판매되는 24개 건어포 및 조미 건어포 제품의 종 판별 및 표시사항 준수 여부를 평가했 다. 이러한 수산가공품에 사용된 원재료의 종판별을 위해 cytochrome c oxidase subunit I 및 cytochrome b 유전자 의 염기서열을 분석하여 NCBI GenBank 및 BOLD 데이 터베이스에 등록되어 있는 생물종의 염기서열과 비교 후 계통 분석을 수행했다. 분석 결과 13개 종(Hyporhamphus quoyi, Gadus chalcogrammus, Lophius litulon, Conger myriaster, Paramonacanthus pusillus, Hyporhamphus sajori, Gadus macrocephalus, Hoplobrotula armata, Callionymus meridionalis, Liparis tanakae, Dosidicus gigas, Lagocephalus cheesemanii, and Takifugu vermicularis)이 확인되었다. 일반 명(장어, 오징어 등)을 포함할 경우 16개 제품(66.7%)에서 표시사항과 판별된 종 간에 불일치가 확인되었으며, 일반 명을 제외할 경우 불일치 비율은 41.7%로 감소했다. 식품 유형별로는 조미건어포 제품(n=20, 70%)에서 건어포 제품 (n=4, 50%) 보다 높은 비율의 불일치 비율이 관찰되었다. 원산지별 분석 결과 특정 국가와 불일치 비율과의 상관성 은 확인할 수 없었다. 이러한 연구 결과는 건포류 제품의 주기적 모니터링 수행 및 수산물의 국명 표시 선을 위한 기초자료로 쓰일 수 있을 것이다.

Moringa oleifera, a versatile plant, has been traditionally used to treat various ailments and is gaining scientific attention due to its potential as a medicine. Native to the Indian subcontinent, it is widely grown in tropical and subtropical regions, thriving in Asia, Africa, and South America, especially in arid climates. This study explores the antioxidant potential of Moringa oleifera leaf extract (MOLE), employing a comprehensive screening approach with various solvents to identify the most effective extraction method. Initial experiments assessed antioxidant efficacy and yield using distilled water (D.W.), 95% ethanol, and 95% methanol. Among these, 95% ethanol extract demonstrated superior antioxidant activity, confirmed through assays such as 2,2-diphenyl-1-14 picrylhydrazyl (DPPH) radical scavenging assay, total polyphenol content analysis, and reducing power assay. In addition, with the 95% ethanol MOLE, a higher extraction efficiency was yielded compared to other solvents, making it the most effective for large-scale preparation. HPLC analysis revealed the presence of key bioactive compounds, including ellagic acid, rutin, Q-3-O, quercetin, and kaempferol. Results revealed that MOLE, prepared using 95% ethanol, exhibited remarkable antioxidant properties, attributed to its rich polyphenolic content. This research underscores the therapeutic potential of MOLE as a natural antioxidant source and highlights the importance of solvent optimization in phytochemical extractions.

In this study, an simultaneous LC-MS/MS multi-residue analytical method was developed and validated for the residues of six neonicotinoid insecticides (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam) in honey. Sample preparation included a combination of QuEChERS extraction kit and liquid-liquid extraction method to effectively extract pesticide components from the honey matrix and optimized analytical conditions to achieve high sensitivity and selectivity. The limits of detection (LOD) and the limits of quantitation (LOQ) were set in the range of 6-15 ng/mL and 19-44 ng/mL, respectively and the correlation coefficient (R²) was greater than 0.99, confirming good linearity. In addition, the intra-day recoveries for each pesticide were 75-104%, and the coefficient of variation (CV) was less than 20%, which met the guideline recommended by the Ministry of Food and Drug Safety. The LC-MS/MS method developed in this study is expected to be used as a multi-residue analysis method for 6 neonicotinoid pesticides in honey.

Clove (Syzygium aromaticum) is a highly valued medicinal plant native to Aisa. Widely used as a spice, renowned for its medicinal properties, particularly in Ayurveda and traditional Chinese medicine. In this study, clove bud extract (CBE) was prepared at different ethanol concentrations of 50%, 80%, and 90%, respectively. The antioxidant activity of the CBE was evaluated through DPPH, polyphenol, and reducing power assays, revealing its strong antioxidant potential, with 90% ethanol being the most effective extract. HPLC analysis identified eugenol (8.7 mg/g) as the major active compound, known to possess anti-inflammatory and antioxidant properties. Given the role of oxidative stress and inflammation in atopic dermatitis (AD), the therapeutic potential of CBE was explored using a 1-chloro-2, 4-dinitrobenzene (DNCB)-induced AD mouse model. Five-week-old BALB/c mice were induced with AD by topical application of DNCB. CBE was administered topically to the affected skin (back and ear) areas for 4 weeks. The treatment of CBE significantly reduced the severity of clinical dermatitis, decreased epidermal thickness, and lowered mast cell and eosinophil infiltration in skin tissue, as observed through hematoxylin eosin staining and toluidine blue staining. The results demonstrated CBE as a promising therapeutic agent for managing AD through its regulation of skin inflammation and oxidative stress, making it a potential candidate for future treatments of inflammatory skin disorders.

Following the previous study, which investigated the pharmacological properties of the Technekitty injection (Tc-99m), the toxicity of a single intravenous administration of the Technekitty injection (Tc-99m) and the side effects that may occur at the diagnostic dose were confirmed. The Technekitty injection (Tc-99m) was administered intravenously once at a dose of 0, 0.67, 2.0, and 6.0 mCi/kg to 5 male and female rats per group. Mortality, general symptom observation, and weight measurement were performed for 2 weeks, followed by observation of autopsy findings. There were no deaths, and no statistically significant weight change was observed. No abnormal systemic signs related to the Technekitty injection (Tc-99m) were observed. These results confirmed that Technekitty injection (Tc-99m) can be safely administered intravenously at doses up to 6.0 mCi/kg. Additionally, technetium-99m at an average dose of 2 mCi (74 MBq) has been verified as a diagnostic dose without adverse effects, allowing the Technekitty injection (Tc-99m) to be used safely without side effects at this dosage. This study demonstrates that the Technekitty injection (Tc-99m) has a wide safety margin, supporting its potential for clinical application. Moreover, these findings align with the nonclinical safety standards for radiopharmaceuticals, reinforcing its utility in veterinary medicine. The Technekitty injection (Tc-99m) is expected to be applicable for clinical diagnosis as a veterinary drug in Korea.

Thyroid scanning using technetium-99m (99mTc) is the gold standard for diagnosing feline hyperthyroidism. In cats with an overactive thyroid, a thyroid scan is the most appropriate imaging technique to detect and localize any hyperfunctional adenomatous thyroid tissue. In this study, the pharmacological properties of the Technekitty injection (Tc-99m), developed as a diagnostic agent for feline hyperthyroidism using 99mTc as an active ingredient, were tested in FRTL-5 thyroid follicular cell line and ICR mice. The percentage of cell uptake of the Tc-99m in FRTL-5 thyroid cells was 0.182 ± 0.018%, which was about 6 times higher compared to Clone 9 hepatocytes. This uptake decreased by 38.2% due to competitive inhibition by iodine (sodium iodide). In tissue distribution tests by using ICR mice, the highest distribution was observed in the liver, kidneys, spleen, lungs, and femur at 0.083 hours after administration, and this distribution decreased as the compound was excreted through the kidneys, the primary excretory organ. Maximum distribution was confirmed at 1 hour in the small intestine, 6 hours in the large intestine, and 2 hours in the thyroid gland. Additionally, the total amount excreted through urine and feces over 48 hours (2 days) was 78.80% of the injected dose, with 37.70% (47.84% of the total excretion) excreted through urine and 41.10% (52.16% of the total excretion) through feces. In conclusion, the Tc-99m has the same mechanism of action, potency, absorption, distribution, metabolism, and excretion characteristics as 99mTc used for feline hyperthyroidism in the United States, Europe, and other countries, because the Technekitty injection (Tc-99m) contains 99mTc as its sole active ingredient. Based on these results, the Technekitty injection (Tc-99m) is expected to be safely used in the clinical diagnosis of feline hyperthyroidism.

Background: Hanwoo cattle farmers aim to improve calf production and reproductive efficiency. Recovery of the reproductive tract postpartum is a critical factor influencing the postpartum period and conception of breeding cows. This study aimed to precisely analyze the recovery process of the reproductive tract in primiparous Hanwoo postpartum and to establish recovery criteria. Methods: Ten primiparous Hanwoo cows were used in this study. After parturition, estrus was examined daily using visual observations and estrus detection patches. Ovarian recovery, cervical diameter, and uterine horn diameter were examined using ultrasonography four times per week. Results: The analysis revealed that the first estrus occurred at 19.1 ± 6.5 days postpartum, the first ovulation at 27.1 ± 4.5 days, and the first normal estrus cycle at 39.2 ± 6.4 days. The ovulation rate during the first estrus was 40%. A normal estrus cycle occurred in 11.1% of patients at the first ovulation. The cervix diameter recovered to 42.0 ± 3.5 mm and the uterine horn diameter to 34.4 ± 7.1 mm by 24 days postpartum, with the difference in uterine horn diameter recovering to 2.6 ± 1.2 mm by 31 days postpartum. Conclusions: This study can aid in determining the optimal breeding time for postpartum primiparous Hanwoo cow and provide foundational data for Hanwoo breeding studies.

대한민국의 해양레저산업은 세일링을 비롯한 다양한 형태의 수상레저 활동이 증가하는 추세에 따라 세계적으로 신흥시장으로 주목받고 있어 세일링 요트에 대한 수요가 높아질 것으로 추정된다. 이에 따라 2022년 현재 우리나라 요트 문화의 부흥과 보급을 위해 해 양수산부의 지원을 받아 28피트 세일 요트의 설계 및 개발이 착수되었다. 개발 초기 단계에서의 성능을 확인하고 이를 통해 설계 인자를 결정하기 위하여 속도 예측 프로그램 VPP(Velocity Prediction Program) 분석이 수행되었다. 본 연구에서 사용된 소프트웨어는 University of Southampton의 Wolfson Unit社의 WinDesign으로, 속도 예측에 적용된 유체역학적 데이터 모델은 Wolfson Unit社자체적인 조선공학 및 요트 연구 분야에서 수십 년간의 예인 수조 시험 데이터를 회귀 분석한 방법으로 대부분의 현대식 요트에 대해 신뢰할 수 있는 추정치를 제공 하는 것으로 여겨진다. 하지만 예인 수조 시험이나 CFD 수치해석 등을 통한 실험 결과적인 유체역학적 정보가 없기 때문에 소프트웨어에 서 제공하는 유체역학적 데이터 회귀 모델의 저항 값은 다소 차이가 있을 것으로 예상된다. 또한, 아직 미완료된 무게 중심 추정에 의한 VCG 값은 속도 예측의 입력 변수 중 하나로, 성능 결과에 어느 정도 영향을 미칠 수 있을 것으로 예상된다. 개발 세일 요트에 대한 최적 화된 보트 속도는 풍속 4, 8, 12, 16 및 20노트의 집세일 조합(최대 120° TWA) 및 스피네커 세일 조합(80° TWA부터) 모두에서 확인되었으 며, VPP를 활용하여 얻어진 최적화된 속도는 국제적으로 유사한 등급의 요트와 견줄수 있는 수준으로 확인되었다.

Background: Platelet-derived growth factor receptor alpha (PDGFRα) is essential for various biological processes, including fetal Leydig cell differentiation. The PDGFRαEGFP mouse model, which expresses an eGFP fusion gene under the native Pdgfrα promoter, serves as a valuable resource for exploring PDGFRα’s expression and function in vivo. This study investigates PDGFRα expression in adult testicular cells using PDGFRαEGFP mouse model. Methods: Genotyping PCR and gel electrophoresis were used to confirm the zygosity of PDGFRαEGFP mice. Histological examination and fluorescence imaging were used to identify PDGFRα expression within testicular tissue. Immunohistochemical analysis assessed the co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 in testicular cells. Results: Genotyping confirmed the heterozygous status of the mice, which is crucial for studies due to the embryonic lethal phenotype observed in homozygotes. Histological and fluorescence imaging revealed that PDGFRα+ cells were primarily located in the interstitial spaces of the testis, specifically within Leydig cells and peritubular myoid cells (PMCs). Immunohistochemical results showed PDGFRα co-localization with c-Kit and ANO-1 in Leydig cells and a complete co-localization with TASK-1 in both Leydig cells and PMCs. Conclusions: The findings demonstrate specific expression of PDGFRα in Leydig cells and PMCs in adult testicular tissue. The co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 suggests complex regulatory mechanisms, possibly influencing testicular function and broader physiological processes.