A twelve-year-old, spayed female Siamese cat was presented for health screening. Abdominal radiography revealed a large amount of mineral opacity substances of various sizes and shapes, along with mild gaseous dilation proximal to the lesion. Ultrasonography showed hyperechoic surface with acoustic shadowing at the ileum and wall thickening of the ileum. Enterotomy was performed to remove the foreign bodies, which were numerous hairball- and stone-like objects. Analysis of the enteroliths revealed a composition of calcium phosphate. Although enterolithiasis is considered rare in cats, it should be included in the differential diagnosis when numerous pebble-like mineral opacity lesions are observed.

Background: Somatic cell nuclear transfer (SCNT) is a prominent technology that can preserve superior genetic traits of animals and expand the population in a short time. Hematological characters and endocrine profiles are important elements that demonstrate the stability of the physiological state of cloned animals. To date, several studies regarding cloned camels with superior genes have been conducted. However, detailed hemato-physiological assessments to prove that cloned camels are physiologically normal are limited. In this study, We evaluated the hemato-physiological characteristics of cloned male and female dromedary camels (Camelus dromedaries). Methods: Therefore, we analyzed variations in hematological characteristics and endocrine profiles between cloned and non-cloned age-matched male and female dromedary camels (Camelus dromedaries ). Two groups each of male and female cloned and non-cloned camels were monitored to investigate the differences in hemato-physiological characteristics. Results: All the animals were evaluated by performing complete blood count (CBC), serum chemistry, and endocrinological tests. We found no significant difference between the cloned and non-cloned camels. Furthermore, the blood chemistry and endocrine profiles in male and female camels before maturity were similar. Conclusions: These results suggest that cloned and non-cloned camels have similar hematological characteristics and endocrine parameters.

A 9-year-old, intact female, Pungsan dog was referred for purulent vaginal discharge and depression. Abdominal radiographs revealed gas-filled and soft tissue opacity tubular structures in the mid to caudal abdomen. On ultrasonography, fluid-filled uterus and cervix accompanied by hyperechoic foci and reverberation artifacts were identified. Multiple hyperechoic foci were found within the uterine wall, indicating gas content. Escherichia coli was isolated from aerobic and anaerobic bacterial cultures. Radiological differential diagnosis of tubular shaped, dilated gas-filled structure, and gas in the wall of the structure should consider emphysematous pyometra with endometrial pneumatosis in intact female dogs with vulvar discharge.

As the 4th industrial revolution emerges, the implementation of smart factories are essential in the manufacturing industry. However, 80% of small and medium-sized enterprises that have introduced smart factories remain at the basic level. In addition, in root industries such as injection molding, PLC and HMI software are used to implement functions that simply show operation data aggregated by facilities in real time. This has limitations for managers to make decisions related to product production other than viewing data. This study presents a method for upgrading the level of smart factories to suit the reality of small and medium-sized enterprises. By monitoring the data collected from the facility, it is possible to determine whether there is an abnormal situation by proposing an appropriate algorithm for meaningful decision-making, and an alarm sounds when the process is out of control. In this study, the function of HMI has been expanded to check the failure frequency rate, facility time operation rate, average time between failures, and average time between failures based on facility operation signals. For the injection molding industry, an HMI prototype including the extended function proposed in this study was implemented. This is expected to provide a foundation for SMEs that do not have sufficient IT capabilities to advance to the middle level of smart factories without making large investments.

Cryopreservation of porcine ovarian tissue by vitrification method is a promising approach to preserve genetic materials for future use. However, information is not enough and technology still remains in a challenge stage in pig. Therefore, the objective of present study was to determine possibility of vitrification method to cryopreserve porcine ovarian tissue and to confirm an occurrence of cryoinjuries. Briefly, cryoinjuries and apoptosis patterns in vitrified-warmed ovarian tissue were examined by histological evaluation and TUNEL assay respectively. In results, a damaged morphology of oocytes was detected among groups and the rate was significantly (p < 0.05) lower in vitrification group (25.8%) than freezing control group (67.7%), while fresh control group (6.6%) showed significantly (p < 0.05) lower than both groups. In addition, cryoinjury that form a wave pattern of tissues around follicles was found in the frozen control group, but not in the fresh control group as well as in the vitrification group. Apoptotic cells in follicle was observed only in freezing control group while no apoptotic cell was found in both fresh control and vitrification. Similarly, apoptotic patterns of tissues not in follicle were comparable between fresh control and vitrification groups while freezing control group showed increased tendency. Conclusively, it was confirmed that vitrification method has a prevention effect against cryoinjury and this method could be an alternative approach for cryopreservation of genetic material in pigs. Further study is needed to examine the viability of oocytes derived from vitrified-warmed ovarian tissue.