This study presents a rapid and sequential radiochemical separation method for Pu and Am isotopes in radioactive waste samples from the nuclear power plant with anion exchange resin and TRU resin. After radionuclides were leached from the radioactive waste samples with concentrated HCl and HNO3, the sample was allowed to evaporate to dryness after filtering the leaching solution with 0.45 micron filter. The Pu isotopes were separated in HNO3 medium with anion exchange resin. For leaching solution passed through anion exchange column, the Am isotopes were separated with TRU resin. The purified Pu and Am isotopes were measured by alpha spectrometer, respectively, after micro-precipitation of neodymium. The sequential radiochemical separation of Pu and Am isotopes in radioactive waste samples using anion exchange resin and TRU resin was validated with ICP-MS system.

This study presents a rapid and quantitative radiochemical separation method for Nb isotopes in radioactive waste samples from the nuclear power plant with anion exchange resin after Fe coprecipitation. After radionuclides were leached from the radioactive waste samples with concentrated HCl and HNO3, the Nb isotopes were coprecipitated with Fe after filtering the leaching solution with 0.45 micron HA filter, while the Sr, Tc and Ni isotopes were in the solution. The Nb isotopes were separated in HCl medium with anion exchange resin. The purified Nb isotopes were measured using a low level liquid scintillation counter after installing quenching curve with standard Nb-94 isotopes. The separation method for Nb isotopes investigated in this study was applied to neutron dosimeter samples from the nuclear power plant after validating the Nb activity concentration with gamma spectrometry system.

Naturally occurring left ventricular hyperplasia is a rare but lethal disease. There are very few reports of this cardiac disease in captive nonhuman primates. In a colony of Macaca mulatta (Rhesus monkey) at California National Primate Research Center, a large number of rhesus macaques were diagnosed by autopsy with naturally occurring left ventricular hypertrophy without obvious underlying diseases over a 22-year period. The confirmatory diagnosis of ventricular hypertrophy was based on findings of notable left ventricular concentric hypertrophy with reduced left ventricular lumen, which is very similar to human ventricular hypertrophy cases. This report discusses an 11-year-old Macaca fascicularis monkey (Cynomolgus monkey, crab-eating macaque), weighing 2.95 kg, that was presented for enrollment in a pharmacokinetic (PK) study. During the PK experiment, the monkey died following a sudden decrease in percutaneous oxygen saturation and heart rate. Gross and histological examinations of the heart were performed. On gross pathology, the left ventricular wall was thickened, and the chamber lumen was reduced. In histopathological examination using hematoxylin- eosin and Masson-trichrome stains, fibrosis and myocyte disarray were not observed, but an increased cell density, compared to the normal heart, was confirmed. The autopsy results confirmed left ventricular hyperplasia as the major cause of death.

The detection of Mycobacterium bovis (M. bovis) in environmental samples with precision is imperative to control bovine tuberculosis (bTB) infections at the herd level, as residual M. bovis remains one of the major causes of recurring infections. In this study, a nested PCR method for the detection of M. bovis in environmental samples was applied to identify potential environmental reservoirs of the bacterium. A set of 200 environmental samples (167 fecal samples and 33 water samples) from 39 herds with a history of bTB outbreak was analyzed using a nested PCR method to detect residual M. bovis. Amplicon libraries of the IS6110 target gene fragment were amplified from M. bovis DNA using two established primer sets. A positive nested PCR result was observed in 69.5% of fecal samples and 66.7% of water samples, thus showing that residual M. bovis was present in the environmental samples of bTB-positive herds in a high proportion. This study is the first to demonstrate high levels of M. bovis DNA in environmental samples and to show that environmental reservoirs of this pathogen contribute to recurring outbreaks of bTB. Environmental monitoring of herds in which bTB outbreaks have occurred with high sensitivity and specificity is expected to help prevent the recurrence of potential bTB disease and improve the herd environment.

The aim of the present study was to investigate the differences in volatile profiles among different Korean chrysanthemum cultivars grown as cut flowers. To optimize a method for sampling and comparing scents, the scents were evaluated by electronic nose (E-nose) at different flowering stages and in different organs and cultivars. The values of maximum resistance changes of metal oxide semiconductor sensors and relative aroma intensity were highest at flowering stage III (full flower stage of ray florets and initial opening disc florets) among different stages and in disc florets among different floral organs of cut chrysanthemums. Among the 12 chrysanthemum cultivars, the highest values for response change and relative aroma intensity were observed in the flowers of the ‘Ilweol’ cultivar. To compare scent patterns among the cultivars, E-nose sensor data was subjected to multivariate statistical analysis. Principal component analysis and discriminant function analysis of the volatile metabolic profile data indicated that chrysanthemum samples were clearly discriminated in a cultivar-dependent manner. These results show that different cultivars are characterized by distinct volatile profiles. Hierarchical cluster analysis showed that the 12 chrysanthemum cultivars were separated into 3 main groups according to the relationship of volatile profiles; however, chrysanthemum cultivars were not clustered according to the flower shape. We propose that these results could be used as the basis on which to improve the breeding of cut chrysanthemums based on volatile characteristics.

한국 땅나리와 섬말나리의 수요는 최근까지도 늘어났지만 종자 상태에서 개화에 이르기까지 확률은 낮은 편으로 자생지에서의 개체수는 해마다 감소하였다. 본 연구는 땅나리(Lilium callosum Siebold & Zucc.)와 섬말나리(L. hansonii Leichtlin ex D.D.T. Moore)의 대량증식을 도모하기 위하여 종자 발아에 유리한 주·야간 온도 조건과 적합한 GA3 농도를 알아보는 것을 목적으로 수행되었다. 땅나리 종자는 2017년 10월 24일 강원도 춘천히 둔산면 원창리(37°47’10.00”N, 127°44’31.00”E), 섬말나리 종자는 2017년 10월 2일 울릉도 울릉군 북면 나리 (37°31’27.06”N, 130°52’25.08”E)에서 채종하였다. 땅나리와 섬 말나리 종자들을 주·야간 15/5, 20/10, 25/15, 20/20°C의 환경 조절상에서 처리구 당 30립씩 3반복으로 치상하여 온도 처리 실험을 진행한 후, 발아율 결과가 가장 우세했던 온도 조건의 환경조절상에서 섬말나리 GA3 처리 실험을 추가로 진행하였다. 온도 처리 실험결과 땅나리는 20/20°C의 온도 조건에서 발아율이 95.0%로 가장 높았으며 평균발아소요일수 또한 10.1일로 가장 낮았다. 섬말나리는 20/20°C의 온도 조건에서 발아율이 78.8%로 가장 높았으며 평균발아소요일수 또한 40.1일로 가장 낮았다. 이를 토대로 20/20°C에서 실시한 섬말나리 GA3 처리 실험은 증류수를 대조구로하여 200, 400, 800mg･L-1의 농도로 상온에서 24시간 침지한 후 처리구 당 20립씩 3반복으로 치상하였다. 그 결과 대조구에서 81.6%로 가장 높은 발아율을 나타냈으며 평균발아소요일수는 200mg･L-1처리구에서 37.1일로 가장 낮았다. 이러한 실험 결과를 바탕으로 땅나리와 섬말나리의 주·야간 발아 최적 온도는 20/20°C이며 GA3 24 시간 처리는 섬말나리 종자 발아에 효과가 없는 것으로 판단 되었다.

This study is investigated the growth characteristics(number of available stipe, pileus diameter, pileus thickness, stipe length, stipe thickness, object weight, comparison yield ability of 1 cycle) and storage characteristics of ‘Sanjo 701’ (S7) cultivars according to relative humidity. The S7 growth characteristics were investigated by quantifying the growth and the characteristics according to the relative humidity, The storage stability was investigated every 5 days and freshness was measured by ‘Minamide Method’. S7 pileus diameter is The higher the relative humidity was confirmed becomes larger and the more increased relative humidity also increase the comparison yield ability of 1 cycle. However, pileus diameter or stipe length This could not see the big difference in the three treatment groups, the plieus thickness was no significant difference in the treatment of 80% and 95%. The fresh weight of S7 decreased significantly at 80% and 95% relative humidity after 10 days of storage, but decreased continuity in 65% humiditiry. The elongation percentage of S7 pileus was observe in 95% relative humidity, values of L, a and b (SCI), showed the highest L value in 65% and the a value in 95%. b values were similar in 65%, 80% and 95% treatments. On the ‘Minamide Method’ measure freshness was changed from the 10th day of storage at relative humidity of 95%, but humidity of 65% and 80% treatments, it changed after 15 days of storage. In this study growth characteristics and yield were increased at higher relative humidity, but storage stability was decreased. Therefore, it is deemed necessary to change the relative humidity to produce high quality mushrooms.

Thrips worms and root-knot nematodes occur in a variety of crops, and have shown a great deal of damage to farm income every year, and the damage is increasing every year. In order to solve these problems, a variety of biological materials are used in Korea to develop a control agent. However, there are very few products available that can satisfy the consumer's satisfactory control effect, efficacy, formulation stability and pesticide compatibility. In order to propose a biological control solution to these problems, this study was conducted to develop the optimal bioprocess technology and formulations suitable for the material by transferring the Aspergillus nigerF22 strain, which is effective for root-knot nematodes, at Chonnam National University. This study was conducted to investigate the efficacy of Aspergillus niger F22 20% suspension concentrate (Productname:NEMAFREE), which has excellent efficacy on root nematodes. The packing test result showed about 70-90% control effect. Soil fumigation and disinfection treatments after 4 days of planting were effective. In addition, we have developed a product to control the under powder pupa using Beauveria bassiana ERL836, an insect pathogenic microorganism, which has excellent control effect against resistant insect pupa. The main purpose of this study was to investigate the effect of insect pests on the under poor control of the pupa in the soil. In the pavement test, more than 70%(GR) formulation, which can be treated withch emical pesticides, and it is confirmed that synergy effect is in the control of Thrips worm.

Recent research has suggested that the dietary protein:carbohydrate (P:C) balance is a critical determinant of fitness in insects. In this study, we examined the effects of dietary P:C balance on life-time reproductive success in the mealworm beetle, Tenebrio molitor. Both males and females lived the longest when fed on P:C 1:1 diet. Throughout their adult lives, females fed on P:C 1:1 diet laid significantly more eggs than those on nutritionally imbalanced diets (P:C 1:5 or 5:1). When given a choice, beetles regulated their intake of protein and carbohydrate to a ratio close to 1:1. Taken together, our results indicate the balanced intake of protein and carbohydrate maximizes life-time reproductive success in this species.

Coffee is one of the most popular beverages all over the world. As the great demand of this product, adulteration coffee are distributed in market mixing fraud materials such as soybean, coffee husk, wheat, rice, corn, malt and barley. But there are no analytical methods for detecting the adulterated coffee. Monosaccharides(mannose, rhamnose, glucose, galactose, xylose, arabinose), trigonelline, nicotinic acid as representative components of coffees can be used as chemical indices to qualitatively and quantitatively assess coffee authenticity. This study carried out on new analytical resources for detection of ground roasted coffee adulteration, investigating model system with roasted soybean, coffee husk, wheat, rice, corn, malt and barley as materials of fraud. It could be concluded that this method was efficient in discriminating different matrixes. Most monosaccharides, trigonelline, nicotinic acids were significant in 5%(w/w) mixed fraud material (p<0.05). These results correspond to monosaccharides, trigonelline, nicotinic acids of pure raw materials, confirming this use as a usefuldetection method of food adulterants in ground roasted coffee.

Effectiveness of Beauveria bassiana ERL as entomopathogenic fungi against thrips pupae were evaluated on greenhouse cucumber(bumnong backdadagi), rose(Affinity) respectively. Control efficacy was calculated by the formula, (Dcontrol - Dtreatment)/Dcontrol x 100, where Dcontrol is the average density of thrips on the plots in which any pesticides and entomopathogenic fungi was not relesed and Dtreatment is the average density of thrips on the plots in which entomopathogenic fungi were released respectively. As a result, control efficacies of Beauveria bassiana ERL 10^7 cfu/g treatment against thrips on cucumber was 89.4~91.3%, respectively. In addition, control efficacy of Beauveria bassiana ERL 10^8 treatment against thrips on cucumber was 92.0~93.2%. Also, control efficacies of Beauveria bassiana ERL treatment against thrips on cucumber was 61.5-69.2%. Thus, even thrips control flow that occurs in the solanaceae vegetables thrips current period is officially Beauveria bassiana ERL 10 ^ 7 to effective treatment cfu / g, pepper and tomato cucumber and rose to occur will be effective. These results demonstrate the promise of entomopathogenic fungi, especially Beauveria bassiana ERL for management of thrips pupae. In addition to thrips control that is occurring in the solanaceae vegetables such as peppers and tomatoes will be effective.