Foot-and-mouth disease (FMD), which affects cloven-hoofed animals, is economically important because of its highly contagious nature. FMD virus (FMDV), the causative agent of FMD, involves seven serotypes (O, A, Asia1, C, and SAT 1-3). Serotype Asia1 is unique to the Asian territory and is subdivided into nine genetic groups (G-I-IX) based on nucleotide variations in the VP1 sequence. Asia1 Shamir, the most representative Asia1 vaccine, is not highly protective against the Asia1/MOG/05 (G-V) lineage found in North Korea in 2007. Therefore, we investigated whether a chimeric virus strain (Asia1/MOG/Shamir), in which the VP4, VP2, and VP3 sequences of Asia1/MOG/05 were combined with the VP1 sequence of Asia1 Shamir, can simultaneously protect against both viruses. We determined the optimal viral growth conditions for the commercial utilization of this chimeric virus strain. Of the three types of cell culture media, the Cellvento medium resulted in the highest amount of antigen in the samples. The chimeric strain was proliferated in a small bioreactor to produce a test vaccine, and its immunogenicity was evaluated in pigs. The virus neutralization (VN) titer against the Asia1 Shamir virus was > 1/100 after the second immunization with the chimeric vaccine in pigs. In addition, a single dose of the test vaccine resulted in a VN titer of > 1/100 against the Asia1/MOG/05 strain. Taken together, our chimeric vaccine strain provided sufficient protection against the Asia1/MOG/05 and Asia1 Shamir viruses, suggesting its potential as a novel vaccine for both these strains.

본 연구는 주변 환경의 차이에 따른 화분매개곤충의 유입 특성을 파악하기 위하여 국립수목원 내 진화속을걷 는정원과 부추속전문전시원에 식재된 울릉산마늘의 화분매개곤충을 조사하였다. 2023년 5월 22일부터 6월 2일 까지 꽃이 70% 이상 개화하였을 때 포충망을 활용하여 8일간 곤충을 채집하였고, 각 전시원 별 식생(피도), 기후 (온도·습도·조도)를 조사하였다. 조사 결과 진화속을걷는정원에서 피도 60% 온도 26.4℃, 습도 31.5%, 조도 40953.6lx, 화분매개곤충 20과 450개체, 부추속전문전시원은 피도 90%, 온도 25.6℃, 습도 31.6%, 조도 6387lx, 화분매개곤충 15과 196개체로 나타났다. 온도와 조도가 상대적으로 높은 진화속을걷는정원이 채집된 곤충의 다양성과 방문 빈도가 높았다. 시간대별 곤충의 방문 빈도를 비교해본 결과 온도와 조도는 개체수가 증가할 때 같이 증가하는 경향을 보였으며, 습도는 반대의 경향을 보였다.

The purpose of this study was to analyze the current status and contents of color-related education programs as lifelong education and to determine the necessity of color education and directions for future development. The findings of this study, first show that the general welfare center, which is striving to develop abilities and promote welfare, was opened mainly for professional education, rather than hobbyoriented education. Accordingly, pricing was set at a lower level than the three institutions and centers, but without a variety of programs or hobby-oriented courses. Second, as the purpose of the set-up is to be established, the general welfare center should be considered an area for hobbies along with expertise, and various courses should be opened. Although the department store’s cultural center focused on programs for cultural and leisure use and promotion, it is necessary to extend the program to attempt to approach it in a short-term process rather than a one-time class. The lifelong education center established as a subsidiary of the university’ was opened as an area for of painting such as oil painting and watercolor and drawing which have much in common school should learn and start basic theory and practice, so there was much difference. Third, if it is changed to a developed form of color-oriented education according to the purpose of each center and institution presented in the results of this research, a good response will be possible to further improve the quality of life and develop professionalism and creativity.

Kombucha is a fermented tea manufactured by adding various microorganisms and sugars to brewed herb tea such as green tea and black tea. Its components and functions vary depending on ingredients, inoculated microorganism compositions, and fermentation conditions. Therefore, this study aims to examine which conditions affect kombucha properties and how these features are affected. Types of substrates, specifically plant-based foods, alter profiles of polyphenol, organic acids, carbohydrates, and protein amounts in kombucha. Long fermentation time raises polyphenol contents and high fermentation temperatures increase sourness in kombucha. Microbial composition of SCOBY, which is the symbiotic culture of bacteria and yeast used to inoculate microorganisms, change the kombucha microbiome that contributes to the chemical composition and functions of kombucha. Several studies have discovered that kombucha has health beneficial functions such as antioxidant activity, hepatic protective effects, antimicrobial effects, anti-diabetic effects, anti-inflammatory effects, and cholesterol reducing effects. These findings indicate that kombucha has high potential as a health functional food. However, future studies are needed to further determine the relationship of manufacturing conditions and functional properties of kombucha.

The serious emergence of chemical-mediated residual toxicity and insect resistance have been enforced the regulation of synthetic pesticides. Future decisions to select more realistic control options probably depend on the speed of technological development in chemical and biological pesticides. Now, a strategic collaboration between synthetic pesticides and biopesticides has been progressed, such as distribution and R&D in collaboration and M&A for obtaining microbial resources. Recently registered microbial pesticides are entomopathogenic fungi in pest management. A concept of e-biopesticide could be properly combined with digital agriculture and accelerate the use of biological control agents in the future farming.

This paper examines a simple one-step and catalyst-free method for synthesizing carbon nanoparticles from aliphatic alcohols and n-hexane with linear molecule formations by using a stable solution plasma process with a bipolar pulse and an external resistor. When the external resistor is adopted, it is observed that the current spikes are dramatically decreased, which induced production of a more stable discharge. Six aliphatic linear alcohols (methanol- hexanol) containing carbon with oxygen sources are studied as possible precursors for the massive production of carbon nanoparticles. Additional study is also carried out with the use of n-hexane containing many carbons without an oxygen source in order to enhance the formation of carbon nanoparticles and to eliminate unwanted oxygen effects. The obtained carbon nanoparticles are characterized with field emission-scanning electron microscopy, energy dispersive X-ray spectroscopy, and Raman spectroscopy. The results show that with increasing carbon ratios in alcohol content, the synthesis rate of carbon nanoparticles is increased, whereas the size of the carbon nanoparticles is decreased. Moreover, the degree of graphitization of the carbon nanoparticles synthesized from 1-hexanol and n-hexane with a high carbon (C)/oxygen (O) ratio and low or no oxygen is observed to be greater than that of the carbon nanoparticles synthesized from the corresponding materials with a low C/O ratio.

본 연구는 자원기반이론 관점에서 우리나라 중소기업의 기술협력 성과를 살펴보고자 하였다. 해당 이론적 관점에서는 기술협력의 동기가 기술적 자원의 보완성이 강조된다는 점을 고려하여 분석 대상의 기업군을 기술수준에 따라 하이테크(high technology), 미드테크(medium technology), 로우테크(low technology) 등 세 단계로 구 분하였으며, 기술협력 파트너를 기술적 자원 및 역량을 기준으로 대기업, 중소기업, 대학, 연구기관으로 구분하고, 각각의 협력관계에 따른 혁신성과를 분석하였다. 분석 결과, 우리나라 중소기업의 기술수준별 그리고 협력 파트너에 따라 기술협력 성과에 미치는 영향은 상이한 것으로 나타났다. 특히 하이테크 중소기업의 경우 대학과의 협력에서 긍정적으로 효과를 확인할 수 있었음에 반해 미드테크의 경우 대학과 대기업과의 협력, 로우테크의 대학과 대기업은 물론 중소기업과의 협력에서도 유의한 성과를 보였다. 비록 기술 수준별로 기술협력의 유의한 성과가 선호체계를 가진다는 흥미로운 결과에도 불구 하고 본 논문이 단년도 횡단면 자료에 근거하고 있는 점을 고려하여 해석 될 필요가 있 다

This study was carried out to investigate insect community structure from different habitats. We performed day and night collection at three different habitats (mountain, coast and rural area) of island Deokjeok, island Soya and island Mungap from May to September in 2014. A total of 3,482 individuals of 725 species, 119 families belonging to 10 orders were collected and identified. A dominant species was Corymbia rubra (Cerambycidae) despite a very low percentage of the species among the catches. Results of ANOVA test showed a significant effect of habitats typeon species diversity. Also, combination of seasons and habitats types were significantly influential with species abundance and species diversity. Indicator species analysis (ISA) result identified 121 significant (p < 0.05) indicator species; one species for the habitats cluster, 93 species for the season cluster and 27species for combination of habitats with seasons.

Epidermoid cyst of the oral and maxillofacial region is a soft tissue cyst lined with keratinized stratified squamous epithelium and most commonly arises in the superficial area of the mouth floor. Uncommonly, the epidermoid cyst may arise deeply in the submandibular region and should be differentially diagnosed with cystic salivary gland tumors, developmental cysts and other cystic lesions. We report a rare case of an epidermoid cyst near the submandibular gland, clinically and radiographically mistaken as a salivary gland tumor.

An insect faunal survey was carried out to investigate insect community structure along the vegetation community to monitor insect species in forest ecosystem. We performed day and night collections from June to August along three vegetation communities of Is. Nam-hae in 2014: the first stand with Pinus thunbergii, the second P. thunbergii with Quercus serrata and the third P. thunbergii with various Quercus species. In total 2,259 individuals of 532 species, 99 families, 13 orders are identified. Cluster Analyses (CA) showed that all three vegetation communities were relatively similar between vegetation community types. According to indicator species analysis (ISA) result, nine significant indicator species were identified (p < 0.05); five species were found to be affected by the vegetation cluster and four species the month cluster.

Intravascular papillary endothelial hyperplasia (IPEH) is a rare benign vascular lesion that rarely occurs in the oral cavity. Its clinical features are similar to neoplasms, which are easily identified in the oral cavity, and it can be misdiagnosed as an angiosarcoma. Therefore, it is important to recognize the characteristics of the lesion in order to both diagnose and treat it properly. We report a case of IPEH in a young Korean female patient, as well as discuss its differential diagnosis and treatment using a review of the related literature.

The silkworm (Bombyx mori), as an industrial insect, possesses a high economic value. Casual discrimination and accumulated genetic information of silkworm varieties are essential ground for the practical utilization and long-term conservation. In this study, nine available microsatellite loci were successfully genotyped from ~50 silkworm strains preserved in Korea. According to genotyping analysis, we obtained 3 ~ 16 alleles per locus, with an average of 7.4, the observed heterozygosity ranging from 0.04 to 0.98, and the polymorphic information content (PIC) ranging from 0.06 to 0.88, revealing that some loci are highly variable. Among 54 strains 13 strains were casually identified by the presence of 17 strain-specific apomorphic alleles. Furthermore, 30 among remaining strains contained strain-specific allele combinations that are also apomorphic to each strain, allowing us to discriminate each of these from other strains by genotyping of multiple loci. These results collectively suggest that the silkworm microsatellite DNA is actually and potentially important molecular marker for the discrimination of the silkworm strains that are preserved as hundreds in Korea, as more loci are genotyped.

본 연구는 온시디움에 있어서 particle bombardment 및 아그로박테리움 매개 형질전환 최적 조건을 구명하 고자 실시하였다. 실험재료는 ‘Sharry Baby’의 PLBs를 사용하였으며, 유전자는 pCAMBIA3301 vector에 삽입 되어 있는 bar와 intron-gus를 사용하였다. Particle bombardment 법에 있어서는 직경이 0.6 μm인 금 입자 에 유전자를 혼합하여 9 cm의 거리에서 1,100 psi 헬륨 가스 압으로 발사하는 것이 유전자 도입에 효과적이었 다. 아그로박테리움 매개법에 있어서는 미성숙 PLBs를 O.D 0.6 정도인 균주에 접종 후 5일간 광조건 없이 공동 배양하는 것이 유전자 도입에 효과적이었다.